جستجوی مقالات مرتبط با کلیدواژه « Vibrio cholerae » در نشریات گروه « دامپزشکی »

Vibrio cholerae is one of the most studied bacterial toxins in the AB5 family and is widely used as a potent mucosal adjuvant because of its ability to enhance the immune response to the antigen injected with it. This toxin consists of two subunits, a pentameric subunit called subunit B, and a subunit A. This toxin enters the target cell by binding to the GM1 ganglionic receptor . In this study Vibrio cholerae were cultured in 100 ml of AKI and Craig's medium in two groups. One group was then incubated at 37 ° C and the other group was incubated at 30 ° C for 72 h. All media were sampled on a regular basis from the beginning of the incubation period to evaluate the toxin production. Finally, the amount of toxin production was evaluated by ELISA GM1 test. The results showed that the bacteria in both culture medium were capable of producing relatively high levels of toxin. The highest toxin production was observed in Craig's medium at 30 ° C after 50 h. The results of ELISA GM1 test showed that the bacterium had the highest production in Craig's medium after 50 hours at 30 ° C, while the highest toxin production in AKI medium after 24 hours at 37° C.

The aim of the present study was to evaluate the antimicrobial effects of aqueous, ethanol, methanol, acetone, chloroform and ethyl acetate extracts of Aloe vera leaves on two fish infectious bacteria, Vibrio cholerae and Listeria monocytogenes. The Aloe vera extracts obtained through rotary apparatus and V. cholerae and L. monocytogenes provided from Iranian fungi and bacterial collection. Minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of the extracts were determined using dilution in well method. Moreover, the diameters of inhibition zones was determined through disk diffusion method. Results of this study showed while aqueous extract of Aloe vera had inhibitory and lethal effects on L. monocytogenes, but have no influence on V. cholerae. The most inhibitory and lethal characteristics of studied extracts on both bacteria was due to chloroform solvent, while had more influence on L. monocytogenes. Chloroform extract tend its inhibitory property even in the most diluted form. Like inhibitory properties, the biggest diameters of inhibition zones was from chloroform extract against L. monocytogenes. Acording to edibility of Aloe vera, its availability and reasonable value, it is possible to use Aloe vera as an alternative for conventional antibiotics and chemical disinfectants in aquaculture rearing pools.