Reputability of a molecular marker linked with rhizomania resistance gene and screening for resistant homozygote gene in sugar beet

In the present study selected indicator from previous studies (non-placental markers AM2), on DNA of single plants of modification mass obtained by cross breeding wild species Beta maritima (having resistance gene Rz2) with sugar beet were called to F2BC1 mass were investigate. To do this first about 1160 seeds of target mass were planted and after one month plants preparation, about 100 Seedling applied to evaluate the phenotypic resistance to the rhizomania contaminated soil was moved to the greenhouse and two months were maintained in this soil. During this period, DNA of plant leaf samples was extracted. Then the quality and quantity of extracted DNA spectrophotometrically was determined. Next, AM2 markers related- primer on single plant DNA with PCR-RAPD method was used to amplify fragments of DNA template. After electrophoresis, RAPD-PCR reaction products in one percent agarose gel and staining gel and observation of the band pattern, presence or absence of markers in a single plant was marked. Then the resistance level of plants cultivated in soil contaminated by the virus concentration in plant roots with Elisa serological tests in the laboratory was determined. Thus the resistant and susceptible plants were identified. The relationship and degree of correlation and agreement between markers (based on molecular data) and resistance (based on ELISA data) in desired single plants was found. By comparing the mean and variance analysis for non-balanced completely randomized design, it was found that the average values of absorbed ELISA of dominant heterozygous and homozygous genotypes has no significant difference. Also comparison of the results for the existence percentage of markers traits as a balanced completely randomized design with four genotypes, including mass F2BC1 (having resistance gene Rz2), Regina (Rhizomania susceptible control varieties), Paulleta (Rhizomania resistant control varieties) and breeding mass S1 (with Resistance gene Rz1) showed that there is no significant differences between genotypes and reproducibility of non-placental markers AM2 in different genotypes was confirmed.