Anti Streptococcus mutans non fluoride and fluoride containing sealants after adding nano-silver particles

Since recurrent caries are one of the major causes of failure in resin restorations، the production of antibacterial resin composites was always under investigation. The aim of this study was to evaluate the efficacy of fissure sealants containing nanosilver particles against the Streptococcus mutans. In this experimental study، the antibacterial properties of two sealants (with fluoride (Clinpro 3M) and without fluoride (Concise 3M) was investigated with 0، 0. 01، 0. 02، 0. 03، 0. 04، 0. 05% w/w after adding nano-silver using direct contact test. Sealants formed on the walls of 500ml micro tube and after curing، they left in contact with bacterial suspension. In periods of 3، 24، 48h، a 10 µl volume of liquid medium was placed in blood agar culture and after 24 h incubation at 37ºC، the number of S. mutans colony was counted by colony counter. Data were analyzed using ANOVA and T-test. Results reported sealants with fluoride comparing to non fluoride ones had significant effect on inhibition of S. mutans growth (P<0. 001). The direct contact test demonstrated that by increasing the amount of nano particles، the bacterial growth was significantly diminished (P<0. 001). While sealants with fluoride demonstrated antibacterial effect، sealants with incorporation of higher weight percentage of nanosilver particles، had stronger and more significant antibacterial effect in direct contact test.