Callus induction in Burdock (Arctium lappa L.)

Burdock (Arctium lappa L.) is an important medicinal plant containing arctiin and arctigenin، which are important secondary metabolits used as anticancer reagents. Due to the existence of important secondary metabolites in this plant، development of a reliable in vitro tissue culture and regeneration methods to facilitate genetic transformation in this plant is important. In the current study، for optimization of the callus induction، leaf، petiole and root explants of A. lappa were cultured in solid MS medium، which was supplemented with 0. 5% PVP (as an anti-oxidant) and different concentrations and combinations of BA and 2،4-D hormones. This study revealed that the petiole explants induced highest callus rate comparing to the other tested explants. In addition، two hormones combined concentrations of 0. 5 mg/L BA + 1. 0 mg/L 2،4-D and 1. 0 mg/L BA + 2. 0 mg/L 2،4-D، produced the highest amount of callus and were the most suitable treatments for the callus induction.