The comparison of Bioavailability of Iranian oral suspension of closantel with reference product in sheep

In the present study, the bioavailability of closantel, a single oral dose of test closantel, 10 mg/kg, was administered to a group of 10 sheep and the same dose was performed by the reference drug (Jansson Company) to another group. Blood samples were collected before administration and 4,8,12,24,48,96,246,432 and 624 hours after administration in both groups of sheep. The serum levels of closantel were determined by liquid phase and C18 cartridges extraction by using a high-performance liquid chromatographic procedure with UV detector. To measures the concentrations, the areas under the peaks were used and pharmacokinetics parameters obtained using non-compartmental analysis. Statistical investigations by ttest showed non significant differences (P≤0.05) in the area under the concentration-time curve, peak serum concentration, time of the peak serum concentration, elimination rate constant, and elimination half-life between test and reference drug. The results showed that the closantel suspension produced by Iranian Company and the reference product was bioequivalent.