Study of Quantitative and Qualitative of B1, B2, G1 and G2 Aflatoxins in Two Species of Aspergillus With HPLC Method

Aflatoxins are the fungal toxins produced by Aspergillus spp. These fungal metabolites in food and oil seed have a big lifetime risk for acute and chronic disease in human and animals. In this survey for production levels of B1, B2, G1 and G2 aflatoxins in two species of Aspergillus was namely A. Alaus and A. Foctides has been investigated. These fungi were grown in Potato Dextrose Broth (PDB) medium and aflatoxins were determined using HPLC method. These species were cultured in 50 ml flask at 26ºc, with 80% humidity and dark place for 32 days. 25 ml of broth cultures were filtered and then extracted with 10 ml chloroform and evaporated the organic solvent and bring the organic solvent in 3ml methanol. After filtration, 20 µl of final solvent was injected to HPLC instrument. The result has shown that A. foetidus produced the maximum amount of B1, B2 and G1 with 8.71, 2.3 & 3.1 mg after 24 days post-inoculation and the maximum amount of G2 with 0.02 after 20 days post-inoculation per 50 ml of PDB. A. flavus produced the maximum amount of B1, B2 and G2 with 487.92, 18.46 &8.57mg after 20 days post-inoculation and the maximum amount of G1 with 126.49mg after 24 days post-inoculation per 50 ml of PDB. Overall the B1 is maximum afltoxin in A. flavus and G2 is the minimum aflatoxin in A. foetidus.