Effect of plant growth promoting bacteria on Seed germination and seedling emergence of alfalfa (Medicago sativa L.) hamedani ecotype in the laboratory, morphological characteristics and pigment contents in greenhouse conditions
In order to comparison the effects of growth promoting bacteria on germination of hamadani alfalfa seeds, a randomized complete block design was conducted with 4 replications and 8 levels of bacterial inoculation (include non-inoculation, application of Acinetrobacter calcoaceticus PTCC 1318, Bacillus megaterium PTCC 1250, Enterobacter aerogenes PTCC 1221 lonely and their pairwise and triple combination of them(. Another CRD factorial experiment was also conducted with 3 replications in greenhouse conditions. Mentioned bacterial treatments were as the first factor and injection of bacteria into the soil of pots as the second factor. The results showed that application of bacteria increased the vigor index of seedling, percentage and germination rate, but did not affect other alfalfa traits in the laboratory. Inoculation of seeds with bacteria in the pots had positive effects on alfalfa growth, percentage and rate of emergence, dry weight, root volume and length, chlorophyll a, b and total chlorophyll content. Injection of bacteria to the soil also affected some traits, which could be due to better placement and/or a larger population of bacteria in the rhizosphere. Application of bacteria lonely had better results than their combinations. It may be due to antagonistic effects of bacteria on each other, which may reduce positive effects.
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