The Effect of Hydroalcoholic Garlic Extract on Hormonal, Clinical Pathology and Histopathological Components in Roosters

The use of garlic as a sexual promotor is common in traditional medicine. Garlic has many antioxidant compounds. Therefore, the aim of this study was to evaluate the effect of garlic hydroalcoholic extract on metabolic and testosterone level, and testicular histology in roosters.

In this experimental study, 108 roosters of Golpayegani breed were used. Roosters were divided into 4 groups including control and treated with doses of 500, 1000 and 1500 mg hydroalcoholic garlic extract in litter of drinking water. The extract was administered orally for 14 days. At the end of the experiment, serum testosterone, T3 and T4 levels, as well as liver enzymes and lipid profiles were measured and testicular tissue was examined for histological changes.

Serum levels of testosterone, T3 and T4 were significantly increased in the group treated with 1500 mg garlic extract in litter of drinking water (P <0.05). Although the amount of liver enzymes in different groups did not show a significant difference, but the amount of triglycerides and cholesterol in the groups receiving different concentrations of garlic extract decreased. In the treated groups with 1000 and 500 mg of garlic extract, the number of seminiferous tubules and the number of normal sperm were higher than the other groups

The biological compounds in garlic with physiological and hormonal changes are able to increase fertility by increasing the density of seminiferous tubules and increasing the normal sperm population.