جستجوی مقالات مرتبط با کلیدواژه « testosterone » در نشریات گروه « زیست شناسی »

Glycyrrhiza Glabra is a Mediterranean perennial plant that its extract has glucose, asparagine, albumin materials, flavonoids, antioxidants, resins and so on, due to the presence of important medical and nutritional compounds, it has been considered by pharmaceutical and food industries. On the other hand, the existence of numerous therapeutic effects of licorice, as well as the identification of the potential protective role of licorice against Covid-19 disease and the increase in its consumption, increases the importance of investigating the effects of licorice consumption on other body systems. This study aimed to investigate the effect of Glycyrrhiza glabra extract on sperm parameters and blood testosterone levels in male rate. Twenty-eight adult male rats, ranging in weight from 180 to 200 g were divided into four groups include control and three treatment groups which every group received 100, 200 or 300 doses of alcoholic extract by gavage daily for 10 days. The results of this study showed that the oral consumption of alcoholic extract of licorice plant at the rate 100 and 200 mg per body weight had no significant effect on sperm parameters and serum testosterone levels (p > 0.05) and only consumption of 300 mg/kg of body weight caused a significant decrease (p < 0.05) in the average number of live sperms and their motility, as well as a significant decrease in the serum level of testosterone was observed in this dose (p < 0.05). But there was no significant effect on the weight of the testicles and the weight of the rats (p > 0.05). It seems that consuming high amounts of licorice extract has fertility-reducing effects in male rats, and this effect can be caused by the reduction of plasma testosterone.

Considering the progress of science and space exploration and the importance of maintaining the health of astronauts both during the space mission and after returning to earth after carrying out space research, investigating the effects of weightlessness on the hormone testosterone and maintaining the fertility of astronauts is very important. is On the other hand, due to the use of carbon nanotubes in the spaceship equipment sector as well as protective shields, astronauts will be exposed to high concentrations of this nanomaterial, hence the investigation of the effects of this nanomaterial on the testosterone level of male astronauts in the conditions Weightlessness is very important in order to maintain their reproductive power. In this research, 30 male rats were used. The animals were randomly divided into five groups of six including control, sham and three experimental groups. The control group received normal food and water for 30 days. The sham group was placed in weightless conditions (in a weightless cage) for 30 days. Experimental group 1 received 20 mg/kg of multi-walled carbon nanotube intraperitoneally for 30 days. Experimental group 2 was placed in a weightless cage for 30 days and received 20 mg/kg of multi-walled carbon nanotube intraperitoneally. Experimental group 3 received 3 cc of solution (2.5 cc of water, 0.5 cc of tween) intraperitoneally for 30 days. The results showed that in the sham group and experimental groups 1 and 2, weight loss was observed. In the examination of blood serum samples, the level of testosterone had decreased significantly. Exposure to multi-walled carbon nanotubes in weightless conditions causes a significant decrease in the amount of testosterone hormone.

In addition to their therapeutic purposes, steroids have been abused a lot and have had many side effects. Therefore, this study aims at investigating the effect of eight weeks of aerobic exercise on structural and biochemical changes in the heart tissue of male Wistar rats receiving therapeutic dosages and abuse of Boldenone steroid. Therefore, 49 male rats with 12 weeks of age and 190 g mean weight were divided into 7 groups: control group, sham, therapeutic dosage (2 mg / kg), abuse dosage (5 mg / kg), aerobic exercise, aerobic exercise + therapeutic dosage and aerobic exercise + abuse dosage. Injections were given in the same day of the week on the back of rats with a graduated insulin syringe and placebo was used. The aerobic protocol included running five days a week for eight weeks on a rodent treadmill at a speed of 30 meters per minute and 60 minutes training. The samples were first anesthetized and blood and tissue samples were transferred to the relevant laboratories. The results were also analyzed by SPSS software and one-way analysis of variance and Bonferroni post hoc test with a significance level of 0.05. The results showed that there was no significant difference between the groups in final weight gain and cardiac troponin (p <0.05). Also, plasma testosterone levels in steroid-receiving rats decreased significantly in both therapeutic and abusive dosages compared to the exercise groups (p <0.05). On the other hand, the amount of running distance in rodent aerobic function test in steroid and exercise groups was significantly higher than other groups (p <0.05). Generally, data showed that aerobic exercise can prevent the reduction of endogenous testosterone but it has no effect on cardiac troponin levels and rat weight. In addition, Boldenone steroid appears to be effective on increasing the aerobic function of rats.

The studies have shown that sex steroids affected cancer cells at the cellular and molecular level. The aim of this study was to investigate the anti-proliferation effects of testosterone on colorectal cancer (HCT) cell lines and caspase-3, -8 and -9 activity level.

In this laboratory-experimental study, cells were randomly divided into control group (no exposure to hormone) and groups exposed to 0.001, 0.01, 0.1, 1 and 10 mg/ml of testosterone. The cytotoxic effect of the extract was measured using MTT assay method. Caspase-3, -8 and -9 activity level was evaluated by ELISA method. The data were statistically analyzed between groups using student’s t-test and ANOVA (Tukey).

0.1, 1 and 10 mg/ml of testosterone significantly reduced the viability of HCT cells compared to the control group (P

Steroid hormones and many plant extracts can modulate pain response. The aim of this study was to investigate the effects of Portulaca oleracea seed hydroalcoholic extract on pain induced by tail flick test in male mice.

In this laboratory experimental study, 150 male mice were randomly divided to control and groups receiving low, moderate and high doses of Portulaca oleracea seed extract and of testosterone and groups receiving “testosterone + extract” of different doses. Following intraperitoneally administration of hormone and extract, pain threshold was measured using tail flick test and data were analyzed using ANOVA.

Increased pain threshold was observed 30 minutes and 1 hour after administration of moderate dose of extract .

Varicocele is one of the most important causes of infertility in men, which in addition to negatively affecting spermatogenesis, can also affect the endocrine system. The link between varicocele and testosterone production is unclear. The liver is one of the most effective organs in the metabolism of substances and hormones. This study aimed to evaluate serum testosterone levels and their association with liver enzymatic changes. Fifty male Wistar rats were divided into 5 groups, including sham control, varicocele induction, varicocele induction (50 mg/kg curcumin), and varicocele induction (100 mg / kg curcumin). After the treatment period, iron (Fe2 +), testosterone, direct bilirubin, total bilirubin and serum concentrations of ALT, and AST were assessed and liver tissue was isolated and examined. Varicocele caused a significant increase in iron levels (p ≤ 0.05) and a non-significant decrease in testosterone levels (p = 0.242) and total bilirubin (T) (p = 0.639). The treatment resulted in a significant decrease in the levels of iron (p = 0.056) and total bilirubin (p = 0.639), as well as a significant increase in testosterone levels (p < 0.05) compared to the varicocele. While the level of direct bilirubin (p ≥ 0.05) in the varicocele showed a significant increase compared to the sham and control. Using curcumin significantly reduced it compared to the varicocele (p = 0.03). Evaluation of ALT (p = 0.762) and AST (p = 0.367) levels did not show a significant difference among the groups. The levels of iron, testosterone, bilirubin, and liver enzymes are negatively affected by varicocele. Curcumin may lessen the harmful effects of varicocele on biochemical components linked with liver tissue in addition to preventing varicocele damage to testicular tissue. In varicocele rats, greater dosages of curcumin may lower the liver enzyme AST.

Many studies have shown the relationship between ultrasound and the function of the reproductive system. However, the mechanism of ultrasound waves action on sex hormones and testicular tissue is not perfectly clear. Accordingly, the present study investigated the effects of ultrasound waves on serum levels of DHEA-SO4, testosterone and testicular tissue in male rats.

Male wistar rats were divided into three groups: control group which was not exposed to ultrasound waves, rats that were exposed to ultrasound waves for 1h/day, and rats that were exposed to ultrasound waves for 6h/day. The serum levels of testosterone and DHEA-SO4 were measured using ELISA. The testicular tissue slides were prepared and analyzed microscopically.Data were analyzed using one-way ANOVA.

Serum levels of testosterone and DHEA-SO4 and spermatogonia, spermatocytes, sertoli cell count and seminiferous tubules morphology did not significantly changed in groups exposed to ultrasound waves compared to control animals.

Our findings show that ultrasound waves do not significantly influence male reproductive system function and histology.

Studies have shown that sex steroids affect the proliferation of cancer cells at cellular and molecular levels. The present study investigated the apoptotic effects of testosterone on lung cancer (A549) cells in cell culture medium. In this experimental-laboratory study, the cytotoxic effects of testosterone on Hek293 and A549 cells were assayed using MTT method. Real time PCR was used to evaluate the expression levels of Bax and Bcl2 genes in A549 cells exposed to IC50 dose of testosterone. The data were statistically analyzed between groups using one-way ANOVA. Exposure of Hek293 and A549 cells to higher dose of testosterone (1000 microg/ml) of testosterone resulted in significant decrease in cell viability (P<0.001). IC50 dose of testosterone significantly decreased the anti-apoptotic Bcl-2 gene expression level (P<0.001) in A549 cells, however, did not significantly change the expression level of apoptotic Bax gene. The cytotoxic concentration of testosterone induces apoptosis in lung cancer cells by its inhibitory effect on anti-apoptotic Bcl-2 gene expression level. Accordingly, appropriate dose of testosterone has anti-cancer effects against lung cancer cells.

The problem of infertility in the world today has become a social concern and can cause severe psychological trauma to couples. One of the most important ovarian cells are granulosa cells that affect the growth and maturation of oocytes. Exosomes are a special class of extracellular vesicles that can trigger cellular responses by signaling to cells. The aim of this experimental study was to evaluate the secretion of estradiol and testosterone in granulosa cells treated with exosomes secreted from bone marrow stem cells. In this study, bone marrow stem cells were cultured and the culture medium of cells containing exosomes was collected. Subsequently, the presence and diameter of exosomes were examined using atomic force microscopy and electron microscopy. Finally, the concentration of hormones secreted from granulosa cells was assessed by ELISA method. The results obtained by atomic force microscopy and electron microscopy confirmed the presence of exosomes with an approximate diameter of 70 nm and also treatment of mice ovarian granulosa cells with exosomes derived from bone marrow mesenchymal stem cells increased the secretion of estradiol and testosterone by these cells. The results of this study confirm the effect of exosomes derived from bone marrow on the secretion of sex hormones and the effect on fertility

Busulfan disrupts testicular function. Given the importance of the effects of leech saliva in the treatment of various diseases, this study aimed to investigate the effect of leech saliva on spermatogenesis and secretion of LH, FSH and testosterone following administration of busulfan in rats. Experimental 60 male Wistar rats aged 2.5 to 3 months with a weight of 200-220 g were randomly divided into 4 groups of 15 (control, leech saliva, busulfan, and busulfan + leech saliva). The busulfan group received intraperitoneal injection of busulfan at a dose of 10 mg / kg twice daily for 21 days. The leech saliva group received 1000 µg / kg leech saliva after 56 days from the first day of the experiment for 56 days. Subcutaneously injected, and the busulfan + saliva group received 10 mg / kg of busulfan saline at 1000 µg / kg saline, subcutaneously after 35 days of the second day of busulfan administration for 56 days. Five rats in each group were anesthetized on day 14, 28, 56 after injection and blood samples were taken from the heart to measure testosterone, LH, and FSH hormones. Statistical analysis of variance (ANOVA) and t-test were considered significant at P

The use of garlic as a sexual promotor is common in traditional medicine. Garlic has many antioxidant compounds. Therefore, the aim of this study was to evaluate the effect of garlic hydroalcoholic extract on metabolic and testosterone level, and testicular histology in roosters.

In this experimental study, 108 roosters of Golpayegani breed were used. Roosters were divided into 4 groups including control and treated with doses of 500, 1000 and 1500 mg hydroalcoholic garlic extract in litter of drinking water. The extract was administered orally for 14 days. At the end of the experiment, serum testosterone, T3 and T4 levels, as well as liver enzymes and lipid profiles were measured and testicular tissue was examined for histological changes.

Serum levels of testosterone, T3 and T4 were significantly increased in the group treated with 1500 mg garlic extract in litter of drinking water (P <0.05). Although the amount of liver enzymes in different groups did not show a significant difference, but the amount of triglycerides and cholesterol in the groups receiving different concentrations of garlic extract decreased. In the treated groups with 1000 and 500 mg of garlic extract, the number of seminiferous tubules and the number of normal sperm were higher than the other groups

The biological compounds in garlic with physiological and hormonal changes are able to increase fertility by increasing the density of seminiferous tubules and increasing the normal sperm population.

In recent years, several studies have been conducted on the properties of medicinal plants. One of the native plants of Iran, which has been highly regarded by researchers, is called wild pistachia. Recent internal studies have shown that the leaf extract of this plant can lower blood sugar in diabetic mice. Therefore, the aim of this study was to investigate the effect of the extract of this fruit on testicular tissue.

Male Wistar rats of approximately equal weight were purchased and housed in the University Animal Room. Animals were divided into 5 groups of 5 in 3 experimental groups, one control group and witness group. Experimental groups received Pistacia atlantica extract at doses of 100, 200 and 400 mg orally and the control group did not receive anything during the experiment.

In the control and control group of sperm-producing tubes with high density, short and very regular distance was seen in testicular tissue and no significant difference was observed. While in the experimental group receiving a dose of 400 mg / kg, the number of sperm tubes was reduced. The amount of testosterone in the 400 mg / kg group was lower than in other groups except control.

The results of this study showed that hydroalcoholic extract of Pistacia atlanticaf in mice reduces testosterone levels and loss of normal state in testicular tissue of these mice.

Alzheimer's disease is a common form of progressive dementia and usually occurs between the sixth and ninth decades of life, given that there is a direct relationship between steroid hormones and neurodegenerative diseases, so the present study was designed to explore effect of testosterone in memory impairment induced by intra-cerebroventricular (icv) injection of streptozptocin (STZ) as a model of sporadic Alzheimer disease (AD). Study was carried out on male Wistar rats. Adult male Wistar rats were intracerebroventricularly (icv) infused with STZ (3mg/kg/icv) on d 1 and d 3, and a passive avoidance task was assessed 2 weeks after the first injection of STZ. Castration surgery was performed in another group of rats, and the passive avoidance task was assessed 4 weeks after the operation. Testosterone (1 mg/kg.d, SC), the androgen receptor antagonist flutamide (10 mg/kg.d, ip), the estrogen receptor antagonist tamoxifen (1 mg/kg.d, ip) or the aromatase inhibitor letrozole (4 mg/kg.d, ip) were administered for 6 d after the first injection of STZ.  STZ administration and castration markedly decreased both STL1 (the short memory) and STL2 (the long memory) in passive avoidance tests. Testosterone replacement almost restored the STL1 and STL2 in castrated rats, and significantly prolonged the STL1 and STL2 in STZ-treated rats. Administration of flutamide, letrozole or tamoxifen significantly impaired the memory in intact rats, and significantly attenuated the testosterone replacement in improving STZ- and castration-induced memory impairment. Testosterone administration ameliorates STZ- and castration-induced memory impairment in male Wistar rats.

The present study aimed to assess the tissue structure and secretions of the male gonads in Arabian carpet shark, Chiloscyllium arabicum Gubanov, 1980 from the Persian Gulf during the spring and autumn. For this purpose, the tissue structure of the testis and the plasma level of testosterone were evaluated during autumn (August-November) and spring (April-June) seasons in C. arabicum. For this purpose, a total of 30 specimen of C. arabicum were caught at the Bahrakan Port, located at the Hendijan in the north of the Persian Gulf, seasonally. Fish were euthanized and blood samples were taken from caudal vein after biometrical tests and then, centrifuged for 10 min. Fish were dissected after bleeding and samples were taken from the testis and fixed in Bouin solution for 48h. Tissue sections were prepared using routine histological techniques and stained with hematoxylin and eosin. Stained tissue sections were studied under light microscope. The amount of testosterone was also measured in plasma samples. The results showed that, the testis of C. arabicum was lobulated and each lobol consisted of golobolar units called spermatocyst with various spermatogenic cells in different developmental stages such as spermatogonia, spermaytocytes and spermatids. Spermatocysts in seven developmental stages were observed in fish testis during spring season. Only spermatocysts in the first five stages of development were detected in the male fish during autumn.

L-carnitine is an essential nutrient. It has vitamin-like qualities and it is considered essential in helping to transport fatty acids into mitochondria. The benefit effect of L-carnitine is proposed for treatment of obesity as long time periods. L-carnitine has a beneficial impact on fertility. Its androgenic activity may by increasing testosterone level that promotes spermatogenic activity. So, the objective of this study was to determine the effects of supplementation of L-carnitine on serum FSH, LH and testosterone levels in adult male rats. Rats were treated different doses of L-carnitine tartarate daily for 16 days, interperitoneally. The control group was treated saline as vehicle. After 16 days, animals were anesthetized with ether and blood specimens were obtained from heart. Serum FSH, LH and testosterone were measured by radioimmunoassay. The results showed that L-carnitine increased serum FSH, LH and testosterone levels in treated animals, significantly. The present study indicated that L-carnitine could effect on sex hormones and reproduction system and also could be an appropriate candidate for improving male reproductive function.

Aim and Curcumin is the yellow pigment derived from rhizome of turmeric. Curcuminoids are natural phenols that used in natural medicine for thousands of years. Currently antioxidant and anti-inflammatory properties of this material are considered in clinical practice. However, a little study that has been done on the effect of curcumin on a reproductive system.
This study was an experimental study, 40 male NMRI mice weighing 20 to 30 grams were investigated. The rats were randomly assigned to four groups: control, treated with 10mg / kg / Day DEHP, DEHP treatment group with curcumin and curcumin group. After two weeks body weight of mice were measured. Right and left testis was weighed separately. The genital pore distance (AGD) was measured. The blood was drived from the heart, testosterone, and dehydroepiandrosterone performed by ELISA Mehod on mice serum. Testicular samples were cutting 5 microns and stained with hematoxilina-eosina method, ultimately were evaluated.
The study demonstrated protective effects of Curcumin on testis of mice. So that daily administration DEHP10mg / kg / day with 5 ml curcumin can improve the parameters of body weight (p = 0.000), testicular right and left (p = 0.000) and serum testosterone (p = 0.026) compared to mice treated with DEHP was alone. Also microscopic pathological waste, including demolition of Sertoli cells and Leydig cell necrosis in the group treated with DEHP was found in the group treated with curcumin DEHP were improved.
Curcumin protects testicular tissue from free radicals induced of phthalates by antioxidant properties. It also modulates the testoster hormone as a regulator of reproduction, weight and growth process.

Inroduction & Sertraline is one of the most widely used antidepressant drug;however ,it has some side effect on different parts of body like endocrine axis.the research have studied the effect of sertraline on the concentration of LH, FSH,Testosterone and Dihydrotetosterone and testicular tissue changes.
Material and In This experimental study 40 adult male rats of wistar were divided in to 4 groups of ten: The control group received normal diet,shame group took group received normal saline as a solvent and experimental groups, received 10, 50 mg per kg body weight serteraline. At the end of 21th days, blood samples and levels of FSH, LH, testosterone and dihydrotestosterone were measured. also preparation of tissue sections of testis tissue. The collected data were analyzed by the SPSS software using ANOVA and ttest.
The levels of LH, FSH, Testosterone ,and Dihydrotestosterone in the experimental groups receiving 50, 10 mg/kg Sertraline was significantly decrease compared to the control and sham groups(P Sertraline causes male sexual imblances by changing in LH,FSH,testosterone and dihydrotestosterone hormone concentration.