Association analysis between germination traits and AFLP markers in rice (Oryza sativa L.)

Rice (Oryza sativa L.) is a staple food for more than half of the world's population. Globally, rice is grown on 161 million hectares, with an average annual production of 678.7 million tonnes. Germination is one of the important stage in rice life. In order to investigate the relationships among AFLP markers and germination traits in rice plants, 192 Rice lines were evaluated. Lines were used in the form of an augument design with 100 seeds per line. The purpose of this study was to identify linked markers for gene controlling plumule length, root length, coleoptile length and germination percentage. 21 primer combinations of EcoRI and MseI a total of 376 bands were produced, of which, 303 bands were polymorphic and had an average of 75.03 percent polymorphism. The most variation in plumule length were determined by E080-M140-11 (19.55%), E060-M160-11 (17.92%) and E080-M150-11 (17.87%).060-M160-11, E080-M150-11, E110-M150-8 and E120-M150-5 Markers were associated with both shoot and root length, which represents an extremely close association of the traits together, or possibly are affected multi-effect genes. Using linked markers with germination traits in this study, we can use markers to help them improve them.