Relationship between the presence of Helicobacter pylori glmM gene and serum fluctuations of IgG and IgA antibodies in infected individuals

The high prevalence of Helicobacter pylori (H.pylori) infection worldwide, especially in developing countries, and its role in gastric malignancies and the emergence of antibiotic resistance have led to proposing the different treatment and prevention methods against infection. HP associated GlmM gene is coding for Phosphoglucosamine mutase that considered for molecular detection of HP.

In this case-control study, blood and stool samples were obtained from studied population and the titers of IgG & IgA were measured by ELISA kits. DNA was extracted from stool samples and PCR was used to detect glmM gene. The results were analyzed by SPSS software.

In initial study regarding of antibodies level in the blood serum, 86% of the subjects in the group showed antimicrobial IgA and IgG at the same time, while in the control group this amount was 59.9%. The results of PCR showed that in the case group, 20 out of 42 cases had glmM gene and in the control group there was no positive sample for this gene.

In this attempt, we tried to find a linkage between HP-associated virulence glmM gene and antibodies concentration through which probability of developing acute and severe state of disease in infected patients. The PCR and Ab titers associated results showed no significant relation between glmM gene of HP originated stool sample and serum concentration of IgG and IgA in patients who suffered from HP infection.