Identification and phylogenetic analysis based on Newcastle disease virus gene F isolated from broiler poultry farms in Markazi province

Newcastle Disease Virus (NDV)is one of the most contagious viral diseases of poultry with rapid spread around the world. It identified by respiratory symptoms, nervous and digestive disorders, and high mortality. NDv causes significant economic losses in poultry industry throughout the world. Avian paramixovirus serotype 1(APMV-1) is a member of family paramyxoviridae, the genus Avulavirus, Newcastle disease virus belongs to APMV-1. The aim of this study to recognize and phylogenic analysis of Newcastle disease Virus isolated from poultry farms in Markazi Province by RT-PCR. In this research, recognition and phylogenic analysis was performed based on F gene of Newcastle disease virus. About 30 samples including brain, trachea, lung and spleen were obtained from suspected farms. Then, They were inoculated to 9 - 11 days age SPF embryonic eggs. After that 2-5 days, The Allantoic fluid harvested, the HA test was performed and genetically analysis using specific primer for F gene was done by RT PCR. In this study, Among the samples 13 of them was confirmed as Newcastle disease virus. Phylogeny tree was constructed for F gene using MEGA 6 software. The results showed that all isolates NDV belonged to genotype VII and subgenotype VII-j. These obtained data can use to improve the efficacy of the vaccine and disease prevention program, as well as its use for vaccine production against the Newcastle-disease causative agents.