Comparison the Effect of 810 nm and 940 nm Diode Lasers on Solobacterium Moorei in Vitro

Given the role of Solobacterium moorei in bacteremia, septicemia, oral infections, halitosis, and periodontal diseases and previous research defects considering the important role of laser in comparison with the application of antimicrobial agents, the purpose of this research was to compare the effect of 810nm and 940nm lasers on Solobacterium moorei in vitro situation.

The research has employed an experimental method. The standard strain of solobacterium moorei bacteria was cultured in a liquid medium (BHI), incubated for 48 hours at 37°C and 10% CO2, and then, the number of bacteria in the liquid medium was diluted to a standard concentration of 1McFarland. The colonies randomly assigned to five groups. In the first group, the control of sterile absorbent paper points was prepared in 50μl bacterial suspension. Initially, it was immersed for 2 minutes, placed on an empty sterile plate, and immersed in sterile physiologic serum. After 30 minutes, all paper points were removed from the plate, immersed in 7ml of Broth letheen medium, and incubated at 37°C for 1 hour. The 810nm diode laser was expose to the second experimental group and the 940nm diode laser was expose to the third experimental group for 10 seconds with 200mv power. Two types of antimicrobials including Chlorhexidine 0.2% and sodium hypochlorite 2.5% were used in the fourth and fifth groups, respectively. Finally, CFU rates were set through standard and bacterial counting methods. The collected data were analyzed by SPSS-20 software and Kruskal-Wallis and Wilcoxon tests.

The CFU of solobacterium moorei was significantly reduced in all the interventional groups (p> 0.05). The reduction of solobacterium moorei in the sodium hypochlorite group, chlorhexidine group, 810nm diode laser group, and 940nm diode laser group were 93%, 92%, 80%, and 60% respectively. 

According to the research results among different interventions, sodium hypochlorite had the highest effect and 940nm diode laser had the least effect on reducing of Solobacterium moorei