Determination of median lethal concentration (LC50) of Vista fungicide in Amur fish (Ctenopharyngodon idella)

The use of pesticides has always been associated with environmental concerns. Vista is a new and widely used pesticide in rice fields for blast disease. The present study investigated the lethal range and median lethal concentration of Vista fungicide in Ctenopharyngodon idella, for the first time. In this study, to determine the lethal range fish were first exposed to the concentrations of 0,10, 25, 50, 100, 250 and 500 mg per liter of Vista, and fish mortality were recorded in each group for 24, 48, 72 and 96 hours. Then, in order to determine median lethal concentration, fishes in 5 groups received concentrations of 0, 30, 35, 40 and 45 mg per liter Vista with three replication, and the number of fish mortality was recorded at 24, 48, 72 and 96 hours. The results were evaluated by probit statistical analysis. In this study, the median lethal concentration of Vista were 60.5, 40.1, 34.58 and 31.28 mg per liter at 24, 48, 72 and 96 hours, respectively. The Maximum Allowable Toxicant Concentration of Vista fungicide was 3.128 mg per liter, and its Lowest Observed Effect Concentration was estimated to be 28.42 mg per liter. The results of this study showed that the lethal concentration of Vista fungicide decreased with increasing test time, so that as the test time increased, a lower concentration of Vista fungicide caused fish mortality.