Fabrication and characterization of liposomal silibinin for wounds healing

This study aimed to develop a liposomal silibinin formulation that would reduce healing time and improve wound healing.

A thin film method was used to prepare liposomal silibinin. They were characterized for their size, ζ-potential, drug encapsulation efficiency, and silibinin release kinetics in vitro. By using a scanning electron microscope, lipid nanoparticle morphology was determined. A MTT assay was then used to determine the cytotoxicity of liposomal silibinin for human skin fibroblasts. SPSS version 18 software was used to analyze the results using one-way analysis of variance (ANOVA).

The optimized liposomal silibinin with a size of 112 ± 9.07 nm, and ζ-potential value of -14.5 ± 2.2 mV exhibited a high silibinin encapsulation efficiency (84± 5.3%). Results of the MTT assay indicated that the liposomal silibinin formulation does not have cytotoxicity besides they promote the growth of fibroblast cells.

Liposomal silibinin promotes the therapeutic effect of silibinin for wound healing.