The effect of eight weeks of crocetin consumption and aerobic exercise on the expression of Myod in the heart tissue of elderly prediabetic rats

Diabetic cardiomyopathy refers to changes in the heart as a result of impaired glucose homeostasis, leading to ventricular dysfunction and associated with mitochondrial dysfunction. Since sports activity is known to protect the heart, the aim of the present study was to investigate aerobic exercise and the effective substance crocetin on the expression of Myod gene in the heart tissue of an experimental model of elderly prediabetic rats.

In this experimental trial, 25 elderly female c57bl6 male mice with prediabetes (20 mg/kg peritoneal injection) aged 14-16 weeks and weighing 30-35 grams were divided into 5 healthy control groups, prediabetic, prediabetic + aerobic exercise, pre-diabetic + crocetin, pre-diabetic + aerobic exercise + crocetin were divided. aerobic training was performed for eight weeks, five sessions per week. Mice received crocetin 30 mg/kg/day by intraperitoneal injection. Myod expression was measured by Real Time PCR method. To analyze the data, one-way analysis of variance and Tukey's post hoc test were used (P≥0.05).

Myod gene expression was significantly decreased in the prediabetic group compared to the healthy group (P=0.01). Myod gene expression was significantly higher in the pre-diabetes group + aerobic exercise + crocetin supplement and diabetes group + aerobic exercise + crocetin supplement than other groups (P=0.01).

It seems that intermittent aerobic exercise and crocetin both alone and simultaneously are effective in improving Myod gene expression in prediabetic heart tissue. Therefore, the use of periodic aerobics and crocetin n is recommended in pre-diabetes conditions of old age