فهرست مطالب محمدرضا دیهیم

Red blood cell (RBC) storage lesions may dramatically reduce the function of these cells. One of these complications is the gradual increase in oxidative stress, which causes the destruction of RBCs during their storage. This study was designed to investigate the effect of glutathione additive as an antioxidant on the biochemical changes of leukoreduced-RBC (LR-RBC) bags during storage.

The study was experimental. In 2016, it was done in Tehran Blood Transfusion Organization. Ten bags of LR-RBC were collected, processed and stored according to standard procedures. Each unit was divided into two equal parts treated with glutathione or normal saline (control). Sampling of all bags was done during the time points of 3, 14, 21, 35 and 42 days after storage. Used the ELISA method to investigate changes in glutathione and 2,3-diphosphoglycerate (2,3-DPG) and the enzymatic method to measure biochemical parameters such as lactate-dehydrogenase (LDH), lactate concentration and glucose-6-phosphate-dehydrogenase (G6PD) enzyme activity.

The amount of glutathione in the treated group and the control group decreased by 70 % and 60% of the initial value, respectively. With the decrease of glutathione level in the last week of storage, a significant decrease in G6PD activity was observed. The amount of 2,3-DPG decreased to almost zero after 42 days of storage. LDH and lactate increased during storage.

Our results showed that, except for the amount of 2,3-DPG, the rest of the studied parameters were affected by glutathione and glutathione antioxidant was effective in reducing oxidative stress

Oxidative damage is one of the main causes of Red blood cell (RBC) storage lesion which can reduce RBC survival during RBC storage in blood bank condition. In this study, we evaluate the effect of N-acetyl cysteine (NAC) as an anti-oxidant compound on RBC oxidative damage and RBC metabolism during storage of this product.

In this experimental study, 10 bag of packed RBC were provided to the Iranian Blood Transfusion Organization's Innovation Center were randomly selected and effect of NAC was investigated on metabolism, oxidative status and hematologic variables of RBC during RBC storage. The results of this study were compared between two groups of NAC treated RBC and untreated RBC (without NAC). All of the data were analyzed with SPSS statistical program (version 22).

In this study, the concentration of lactate and LDH enzyme activity in the NAC treated RBC were lower than the control group (without NAC). The concentration of malondialdehyde (MDA) as a lipid peroxidation marker in the NAC treated RBC was lower increase than the untreated RBC. Also antioxidant capacity was so higher in the NAC - RBC than in the control group, especially in the 28th day of RBC storage (Pday28 <0.05).

The results of this study indicated that the use of NAC as an additive solution could decrease oxidative damage via maintaining of the RBC oxidant capacity during RBC storage. In the future NAC may be used as an additive for maintaining of the RBC survival and RBC quality during storage of RBC in blood bank condition.

Platelets undergo a series of biochemical, functional, and morphological changes during storage that are collectively called platelet storage damage, which can reduce platelet function and survival. Oxidative damage is one of the leading causes of platelet storage damage. Therefore, in the present study, an attempt has been made to determine the effect of oxidative stress on platelets during storage. This research was conducted in 2017 at the Research Center of the Iranian Blood Transfusion Organization.

In the current experimental study, 10 platelet concentrate bags prepared by platelet-rich plasma (PRP) were examined. Research parameters include measurement of mitochondrail reactive oxygen species (ROS), concentration of malondialdehyde (MDA), concentration of nitric oxide metabolites (nitrate / nitrite), activity of lactate dehydrogenase enzyme (LDH) and platelet count on days 1 ,3 ,5 and 7 of platelet storage. Statistical analysis of variance was used to describe the obtained data

According to the results, ROS (16.22±37), MDA (11.6±22.2) concentration and LDH enzyme activity (310±3680) increased significantly (P<0.001) while PLT count was significantly decreased (P<0.001).

It seems that oxidative damage occurred in platelet with increasing radical oxygen species and lipid peroxidation which can reduce platelet count and platelet viability during platelet storage. Using antioxidant as an additive in platelet concentrate may decrease oxidative damage and increase platelet viability and quality during storage.

There is a circadian rhythm and diurnal variation in the redox capacity in blood plasma and there is a decrease in antioxidant capacity (TAC) during the RBCs storage in blood banking. So it is possible that donated blood, based on donation time, has a different antioxidant capacity and as a result a different biochemical response during storage. This study attempts to uncover this issue.
 

In this experimental study, Twenty RBC units were collected from two donor groups in the morning (8-11am) and evening (5-8pm) and were stored for 3 to 42 days at 4 to 8 degrees Celsius. Glucose, Sodium, potassium, lactate, lactate dehydrogenase (LDH), pH, malondialdehyde (MDA), and total antioxidant capacity were measured by a commercial kit. Nitrate/nitrite was measured by Griess method.
 

The levels of nitrate/nitrite, MDA, and LDH in the evening group increased significantly when compared to the morning group (p < 0.05). However, a decreasing trend in TAC, pH, Glucose, and sodium levels (p < 0.05) versus an increasing trend in potassium, lactate, LDH, and MDA was seen in both groups during storage (p < 0.05).

This study showed that oxidative damage in donated blood in the evening was higher than in the morning during storage, which may reduce RBC survival. Therefore, more studies are required with more sample sizes to examine the effect of donation time on the RBC storage lesion.