مقالات رزومه دکتر عبدالغفار اونق

The killed preparation of microorganisms in combination with alum does not generally produce strong cellular immune responses. Glycyrrhizic acid is a triterpenoid saponin with immunomodulating properties. The present study was conducted to investigate the synergism of glycyrrhizic acid (GL) and alum liposome on immune responses against the killed form of Salmonella Typhimurium (HKST).

In this experimental study, male Balb/c mice in 5 groups of 15 were immunized with HKST vaccine alone or in combination with alum, GL or alum-GL combination twice with two weeks’ interval. Fourteen days after the last vaccination, the immune responses against Salmonella Typhimurium and the protective potential of the vaccines were evaluated. Survival rate was monitored by Kaplan-Meier analysis. Other findings were analyzed using one-way analysis of variance and Tukey's post hoc test. A level of P<0.05 was considered significant.

Delayed type hypersensitivity reaction, lymphocyte proliferation, IgG2a antibody titer and the rate of improvement of survival against challenge with live were significantly increased in the group receiving combined adjuvant and HKST compared to other groups. In the group receiving combined adjuvant, the level of IFN-γ significantly increased and IL-4 significantly decreased more profound than other groups.

Alum-GL combination as an adjuvant synergistically increased cellular and humoral immunity after immunization with the HKST vaccine.

Thymus vulgaris and silver Nanoparticles has been demonstrated. Each of these agents for maximum impact requires a high concentration of substances affecting microorganisms. Assuming an anti-microbial agent with low concentration and in combination with other anti-microbial agents as the initial impact or more, A study to evaluate the effect of ethanol extract of propolis interference, Thymus vulgaris essential oil and silver nanoparticles on gram-positive bacteria (Staphylococcus aureus, Streptococcus pneumoniae, Listeria monocytogenes) and Gram-negative bacteria (Proteus mirabilis, Klebsiella pneumonia and Pseudomonas aeruginosa) and on Candida albicans was performed. Microbial used in this study are taken from Iranian Research Organization for Science and Technology. Propolis is collected from different area of west Azerbaijan beehives. The ethanolic extract of propolis have prepared after 3 days. The interfering effects of these agents are evaluated by Micro-broth dilution method on Muller Hinton Broth in the presence of gentamicin as positive control for bacteria and Nistatin against Candida albicans. Acquired Minimum Inhibitory Concentration (MIC) show the effective interfering of these agents. The results of the Minimum Inhibitory Concentration (MIC) of propolis extract with silver nano particle on Klebsiella pneumonia was 25 µgr/ml, on Streptococcus pneumonia, Pseudomonas aeruginosa and Candida albicans was 50µgr/ml and on Staphylococcus aureus, Listeria monocytogenes and Proteus mirabilis was 100 micrograms per milliliter. MIC of Thymus essential oil with silver nanoparticles on Klebsiella pneumonia and Pseudomonas aeruginosa was 50µgr/ml, on Staphylococcus aureus, Streptococcus pneumonia and Candida albicans was 100 µgr/ml and on Listeria monocytogenes and proteus mirabilis was 200 micrograms per milliliter.

The issue of antibiotic resistance is currently being pursued very seriously. One of the drug-resistant bacteria is Pseudomonas aeruginosa. As an opportunistic and important pathogen in human and livestock, this bacterium can be resistant to various antibiotics in different ways. For thisreason, research to obtain antimicrobials from alternative sources such as plants and plant essential oils is considered by many researchers. This study investigated the antimicrobial potentials and therapeutic effects of pulegone in experimental cutaneous pseudomoniasis in goats clinically.

Four two-year-old female goats weighing approximately 45 kg were used. To prepare the P. aeruginosa inoculation site, the right and left chest and the flank on both sides of the animal were shaved to an area of 30 cm2 at each site. After supressing the immune system by injection of dexamethasone, in each place at five points and at each point 0.4 ml of P. aeruginosa suspension, was injected intradermally. After cutaneous pseudomoniasis induction, lesions in the right thoracic region were treated with glycerol-soluble pulegone, lesions in the left thoracic region were treated with gentamicin ointment as standard treatment, and lesions in the right thoracic region were treated daily with glycerol. Lesions in the left flank were not treated as a control group.

The results of this study showed that pulegone, just like gentamicin, treats goat cutaneous pseudomoniasis wounds in nine days and heals them effectively.

Candida albicans is a natural opportunistic flora on the skin and mucous membranes, growth in good conditions and causes clinical symptoms of candidiasis. Prophylactic and therapeutic administration of some antifungal agents has been caused drug resistance, so nowadays there is a need to introduce newer drugs for the treatment of candidiasis. In this study, 12 heads of 8 months old goats with a weight of 20 kg were used. After induction of vaginal candidiasis with Candida albicans (PTCC: 5027), animals were randomly divided into four groups: treated with ethanolic extract of propolis, treated with nystatin ointment, treated with glycerin and non-treated group. In this study, mucosal injuries occurred within 5 days. Ethanol extract of propolis during 5 days and nystatin within 7 days resolved the clinical signs of vaginal candidiasis. Mucosal injuries created in non-treated and glycerin-treated groups worsened over time by up to 3 weeks later. In the histopathological examination, the anti-inflammatory effects, the repair of sweat glands, sebaceous and hair follicles, and the removal of abscess from the dermis, in the treatment groups with ethanolic extract of propolis and nystatin, were significantly different from that of the non-treated and glycerin-treated groups. The results of this study showed that the ethanolic extract of propolis can repair vaginal candidiasis lesions in a shorter period compared to nystatin in goat.

Pseudomonas aeruginosa is one of the most important opportunistic pathogens. Due to the increased use of antibiotics and the risk of their resistance, studies on the detection of natural antibacterial compounds have increased. Hence, the aim of the present study was to determine and evaluate the antibacterial effect of propolis nanomaterials, ethanosybenic anodic anodomic extracts of propolis, ciprofloxacin.

In the present experimental study, which was conducted at the University of Urmia in 2018, high-energy propolis nanoparticles were prepared using ultrasonic waves. Then the antimicrobial effect of propolis ethanolic extract, propolis nanoamulsion alone, ciprofloxacin alone and their combined combination (propolis + ciprofloxacin, propolis cyanide + ciprofloxacin nanoparticles) by microenvironmental microenvironmental microenvironmental and microbial methods Pseudomonas aeruginosa was diagnosed. Data were analyzed using one-way variance test. In this study, high-energy propellant nanoemulsion was prepared using ultrasound waves. Antibacterial effect of ethanolic extract of propolis, propolis nanoemulsion, Ciprofloxacin alone and their combinations (EEP+Ciprofloxacin and propolis nanoemultion + ciprofloxacin), were determined. For determination of MIC, MBC and FIC of each substance, Broth microdilution and Disk diffusion test were used. Data Statistical analysis was performed using SPSS software and one-way ANOVA test.                                                                                                                                                                                                                                                                                                                                                                                    

First, 8,156 nm of propolis extract was prepared from propolis nanoparticles. The results of MIC and MBC experiments indicated that the minimum inhibitory concentration of alcoholic propolis extract extract and propolis nanomaterials against Pseudomonas aeruginosa was 1000 and 46.88 micrograms per milliliter, respectively. Moreover, the evaluation of the combined effect of propolis extract and propolis nanomaterials with ciprofloxacin is 9.46 2 2.44, 7.3 22 1.32 μg / ml, respectively. Furthermore, platelets containing combined propolis nanoamulsion dissociation disks with ciprofloxacin with concentrations of 1.32 7 3.7, micrograms per milliliter of halo growth, respectively, caused a growth of 15 mm in diameter, while single-use nanograms per 6 milli6 milligrams of propolis The growth control halo was 15 mm larger.

In the present study, for the first time, nanoamulsion was prepared from the ethanolic extract of propolis, which alone and in combination with ciprofloxacin has the effects of inhibiting growth inhibition and high elongation on Pseudomonas aeruginosa. It had a synergistic effect and as a result, the dose of each of the compounds and the time required to kill the bacterium was reduced by comparing the use of each of them alone.

During the history, researchers studied the healing effects of traditional medicine against many infectious diseases. In order to explore therapeutic role of Propolis Ethanolic Extract (EEP), an experimental cutaneous candidiasis was induced in goat model as InVivo study.

Four young healthy goats without any skin fungal infection were selected from different herds. The skin on both sides of the lateral thoracic and flank areas of the studied animals were shaved. The Candida albicans PTCC 5027 suspension were inoculated on each site. On day 8th, 64 large abscesses and pimples were appeared on all inoculated sites. All treatments begun on 8th day. Wideness of Cutaneous Candidiasis lesions on all sites of all goats scaled by a ruler during this study in centimeter and monitored planimetrically and analyzed statistically. Biopsy were collected on particular days under local anesthesia after intradermal inoculation date. Sections were stained with hematoxylin and eosin (H & E) and special stain of G.M.S. for fungal infection.

Comparison of the values for different treatments revealed a significant reduction in abscess size and inflammatory signs in EEP treated group comparing to Nystatin treated , Glycerin treated and untreated groups (P≤0/05). Histological findings showed anti-inflammatory results, with better cutaneous repair, sebaceous, sweat gland, and hair follicle regeneration in EEP group, better than other treatment groups (P≤0.05).

EEP has a considerable anti-inflammatory and antifungal effects even more better than Nystatin ,without its side effects (scar formation, shedding of infected crust, "treatment dependent hypersensitivity").