hoda shahiri tabarestani

Production of probiotic (PRO) cereal-based products is an important strategy to increase consumption amounts of these beneficial microorganisms in our daily diet worldwide. In the present study, textural features, sensory properties and viability of an adjunct PRO culture were determined in supplemented cupcake with fermented black rice (FBR) and coated with malt. Based on the obtained results, survival of the PRO yeast in malt edible coating reached to 106 CFU/g after 6 days of storage in cupcake samples supplemented with FBR. In addition, crumb hardness (1529.77 g) and gumminess (962.14) of the produced cupcake supplemented with FBR were significantly (P<0.05) higher than those of the control sample. Meanwhile, proper porosity and overall acceptability were observed for the aforementioned FBR added cupcake. Accordingly, application of a potential PRO yeast via malt coating as a proper carrier can maintain viability of this adjunct culture during shelf-life period of the product in the recommended dose for PRO products. Considering the health-promoting potentials of PRO and consumed amounts of cupcake, it is a good choice to consume a high dose of PRO in our today’s diet style.

Soy whey (SW) is a byproduct from tofu and soy protein isolate (SPI) production that contains various nutrients such as protein, amino acids, minerals, carbohydrates, isoflavones. In this study, SW was fermented with lactic acid bacteria (LAB) with the aim to enhance total phenolic contents (TPC), Gamma amino butyric acid (GABA) and antioxidant activity. Eight different LAB strains were selected and the activity and cell counts of the most potent strains were investigated during fermentation. The results showed that all the isolates were able to grow in SW and the increase in incubation time led to significantly (p<0.05) decrease the pH of all samples from 5.75 to 4.5. Among eight LAB isolates, Lactiplantibacillus plantarum MCM4 and Weissella confusa MDM8 showed higher activity in terms of acid production, increase in TPC content and proteolytic activity. The sample fermented by L. plantarum MCM4 had the highest content of free amino acids (1.73 mg/ml) and the unfermented sample with 0.9 mg/ml had the lowest content. GABA concentration varied from 6.15 mg/mL (unfermented) to 24.175 mg/100 mL (SW fermented with L. plantarum MCM4). In this research, it was found that fermentation increased the antioxidant capacity of SW in such a way that the highest amount was observed in sample fermented with Lactiplantibacillus plantarum MCM4. A positive correlation (R2= +0.72) was found between viable cell counts and proteolysis. It can be concluded that, fermentation with L. plantarum MCM4 and W. confusa MDM8 can be applied as an approach to valorize SW.

Astaxanthin, an orange-red carotenoid pigment belonging to the xanthophyll group, is characterized by the presence of carbon, hydrogen, and oxygen atoms in its structure. Within oxygenated carotenoid derivatives, it holds the highest concentration. Astaxanthin sources can be categorized into two main groups: natural and synthetic. Extensive research has focused on the recovery of carotenoids, including astaxanthin, from solid by-products of shrimp and crustaceans. This interest stems from its potential applications in pharmaceuticals, chemicals, food, and animal feed industries, attributed to its notable coloring and antioxidant properties. This study aims to compare the extraction of astaxanthin from the shells of shrimp (Fenneropenaeus merguiensis) and Gammarus (Pontogammarus maeoticus). Two methods, the traditional soaking method and an ultrasound method utilizing a microemulsion of ionic liquid in water, were employed for the extraction process

The initial step involved the preparation of sample powder using a freeze dryer. Subsequently, the extraction process was initiated by combining the prepared samples with an ionic liquid microemulsion in water, maintaining a 5:1 ratio of solvent to sample. Two distinct methods were employed for extraction: the traditional soaking method conducted at room temperature for 24 hours and the ultrasound method, carried out at ambient temperature, utilizing a power of 60 watts for a duration of 30 minutes. The assessment of astaxanthin extraction was conducted through a spectrophotometer. Various parameters, including astaxanthin extraction amount, total carotenoid amount, extraction efficiency percentage, and antioxidant activity, were scrutinized. The antioxidant activity was measured using the DPPH method

The ultrasound method proved to be highly effective in extracting astaxanthin from shrimp (Fenneropenaeus merguiensis), yielding a maximum amount of 76.30 ± 1.09 mg/ml. This result highlighted shrimp (Fenneropenaeus merguiensis) as a superior source for astaxanthin extraction compared to Gammarus (Pontogammarus maeoticus). Moreover, the study demonstrated the superior efficiency of the ultrasound method over the soaking method, with extraction efficiencies for shrimp (Fenneropenaeus merguiensis) calculated at 95% and 59%, respectively.The total carotenoid content for shrimp (Fenneropenaeus merguiensis) was measured at 77.98±1.33 ml/g and 79.77±0.46 ml/g using the soaking and ultrasound methods, respectively. Notably, the antioxidant activity of astaxanthin extracted through the traditional soaking method surpassed that of the ultrasound method. However, when compared to the synthetic antioxidant BHT, astaxanthin exhibited lower antioxidant activity at increasing concentrations in both methods.In summary, the ultrasound method demonstrated its superiority in astaxanthin extraction, with shrimp (Fenneropenaeus merguiensis) emerging as a more favorable source. Despite its lower antioxidant activity compared to BHT, astaxanthin's natural origin and extraction efficiency make it a promising candidate for various applications.

In general, the results of this research showed the importance of waste and new methods in extracting valuable compounds. In the context of extracting astaxanthin from two selected sources, shrimp (Fenneropenaeus merguiensis) emerged as a superior source compared to Gammarus (Pontogammarus maeoticus). Furthermore, the study highlighted the ultrasound method as a promising alternative to traditional extraction methods, exhibiting superior performance and efficiency. These findings contribute to the broader understanding of sustainable practices and advanced techniques in maximizing the extraction of valuable compounds from natural sources.

 Astaxanthin is a widely used carotenoid pigment in the food industry which is extracted from various natural and synthetic sources. Nowadays, due to the adverse effects of organic solvents green solvents which are non-toxic, non-volatile and environmentally friendly have been proposed. Therefore, this study focuses on comparison of the extraction of astaxanthin from shrimp (Fenneropenaeus merguiensis) and Gammarus (Pontogammarus maeoticus) under soaking conditions for 24 hours with organic solvent (combination of ethanol with ethyl acetate), green solvent (microemulsion of ionic liquid in water) and vegetable oil (sunflower oil). Ionic liquid microemulsion in water is considered a newnovel solvent for astaxanthin extraction. Determination of density, conductivity and diameter were the characteristics of microemulsion test. In extraction,Solvent to sample ratios of 5x, 12.5x and 20x were used for the extraction and compared with the control sample.

merguiensis and P. maeoticus With species approval were procured from Persian Gulf Ecology Research Institute (Iran). Commercial astaxanthin (>98 % purity), α-diphenyl-β-picrylhydrazyl (DPPH), and butylated hydroxytoluene (BHT) were procured from Sigma-Aldrich (USA). The HPLC grade ethanol, propanol, ethyl acetate,, tributyl octyl phosphonium bromide, Triton X-100, and n-butanol were obtained from Merck Chemicals Co. (Germany). Refined sunflower oil which was antioxidant-free, was also purchased from Hayat Company (Iran). The shell of F. merguiensis and P. maeoticus were carefully washed with distilled water, then freeze-dried (Christ-Alpha 1–4, LD freeze dryer, Germany) for 48 h at -50 °C. After sieving the powders with a laboratory sieve with a mesh smaller than 15 µm. The obtained powders were kept at Refrigerator. All experiments were done in the Food and Drug Administration Department of Hormozgan University of Medical Sciences.

 According to the results, the density of the microemulsion was determined in the range of 0.97151 g/cm3, its diameter was 15.8 nanometers and the conductivity was 312 microsiemens at 27.1°C. The results of astaxanthin extraction with different solvents in the comparison with control solvent were statistically significant (p< 0.05). According to the results obtained from the extraction of astaxanthin from two sources of shrimp and gammarus, shrimp was selected as the source with the highest amount of extracted astaxanthin. The use of green solvent (ionic liquid microemulsion in water) in a ratio of 12.5 times solvent to sample was also chosen as the optimal method. The amount of astaxanthin extracted under optimal conditions was 77.44 ± 1.09 mg/ml. The results of DPPH radical inhibition by extracted astaxanthin using ionic, oily and organic solvents compared to synthetic antioxidant BHT showed that the antioxidant activity increased with increasing the concentration of astaxanthin, but this increase was always lower than BHT.

In general, the results of this research show that the use of microemulsion based on ionic liquids is a suitable alternative to conventional methods in extracting and recovering astaxanthin from natural biological sources.

Seeds and nuts have received increasing attention due to their nutritional value and the high therapeutic properties of their bioactive compounds. Most of the seeds are used as nuts, and some of them are considered agricultural waste. Pumpkin seeds have a high content of protein (30–40% in terms of dry matter). Proteins are among the vital health-giving components that provide nitrogen, essential amino acids and energy necessary for normal cells. Pumpkin seeds are a good source of amino acids such as valine, histidine, isoleucine, leucine, threonine and methionine. Protein hydrolysate is a mixture of peptides and amino acids that can show antioxidant, antimicrobial, anticancer, antidiabetic and antihypertensive properties. During hydrolysis, proteins are broken into small peptides and amino acids. Since enzymatic hydrolysis is performed in relatively mild conditions and no amino acid damage occurs, this type of hydrolysis is preferred over acid and alkaline hydrolysis. Hydrolysates obtained from pumpkin seed protein have bioactive properties, especially antioxidant activity. Pretreatment of proteins before enzymatic hydrolysis acts to improve the release of bioactive peptides from different proteins. Pretreatment can facilitate the unfolding the structure of proteins and thus increase the access of enzymes to peptide bonds. The main properties of microwaves usually show three characteristics: penetration, reflection and absorption. Microwave assisted enzymatic hydrolysis can shorten the time  and improve the speed of the reaction. The purpose of this research was to investigate the antioxidant activity of pumpkin seed protein hydrolysates (Cucurbita maxima L.) by alcalase enzyme in two conditions: without pretreatment and using microwave pretreatment.

In this study, Pumpkin (Cucurbita maxima L.) was purchased from the local market of Astane Ashrafieh in Gilan province. The seeds were scooped manuallyand then dried in an oven at 50°C for 72 hours. After the production of protein concentrate from pumpkin seeds, the chemical properties of the concentrate, such as the amount of fat, protein, ash and moisture, were measured. The isolated pumpkin seed solution was exposed to microwave energy with a power of 450-900 watts for 30–90 seconds and was used as a substrate solution in enzymatic hydrolysis experiments. It should be noted that after measuring the total antioxidantactivityr for different powers and times of microwave pretreatment, the power of 600 watts for 60 seconds was selected  and applied before enzymatic hydrolysis. Enzymatic hydrolysis was done by alcalase enzyme with a concentration of 0.5 to 2.5% compared to the protein substrate during 20 to 190 minutes, and the optimum temperature and pH of alcalase were determined in order to produce hydrolysates with antioxidant activity. Antioxidant activity was measured by using DPPH free radical inhibition, total antioxidant activity and iron chelation activity methods.

Bioactive peptides produced by the enzymatic hydrolysis of proteins have significant antioxidant properties. Pumpkin seeds can be used as a rich source of nutrients and bioactive compounds in various food industries. The results showed that the maximum amount of antioxidant activity without pre-treatment was achieved in 165 minutes with a 2.2% ratio of E/S by using DPPH free radical scavenging activity (40.5%), total antioxidant power (0.79), and iron chelation activity (96.2%) methods. By using microwave pre-treatment, the maximum amount of antioxidant activity was achieved in a shorter time and with less enzyme (105 minutes and E/S ratio 1.5%) using DPPH free radical scavenging (52%), total antioxidant power (0.711), and iron chelation activity (93%). Therefore, it can be concluded that using microwave assisted enzymatic hydrolysis , in addition to achieving hydrolysates with proper antioxidant activity, is a suitable method to save time and reduce enzyme concentrations used in enzymatic hydrolysis.

Gelatin, a protein-based hydrocolloid widely used in food and drug industries, faces limitations when derived from cold-water fish species due to low gel strength and functional properties. In this regard, this study explores the potential of oak (Q.castaneifolia) phenolic extract as a natural modifier of commercial cold-water fish gelatin (CW-FG). The use of natural crosslinking additives especially oxidized forms of phenolic extract may be suggested as the modification strategy of gelatin hydrogel. Therefore, the objective of this study was to evaluate the potential of oak (Q.castaneifolia) extract as chemical and rheological modifier of commercial cold-water fish gelatin.

The scope of the current work was to investigate the effects of concentration of oxidized/unoxidized oak phenolic extract, reaction pH values (7 and 9), and different concentrations ranged from 0.1 to 0.9 (mg/g protein) on gel strength, crosslinking degree, color parameters and swelling ratio of commercial cold-water fish gelatin. Further characterisation of the properties of different gelatin hydogels was also conducted by their conformational changes using fourier transform infrared (FTIR) analyses and scanning electron microscopy (SEM).

The modified CW-FGs demonstrated higher gel strength, reduced swelling ratio and free amino group, and a more compact structure compared to uncross-linked samples and those containing unoxidized oak phenolic extract at neutral pH. The addition of both types of oak acorn extracts at increasing pH levels led to a decrease in L*-value and an increase in a* and b*-values. FTIR analysis revealed hydrogen bond formation as the primary molecular interaction, particularly in CW-FGs modified with oxidized oak extract at pH 9. Additionally, free amino group could be controlled by adjusting the concentration of oak extracts. SEM analysis revealed that CW-FG gel containing Ox-OPP exhibited a non-uniform surface with varying pore sizes, exhibiting morphological differences from CW-FG gel containing OPP, particularly characterized by the creation of large empty spaces and fine filaments. These findings collectively demonstrate the potential for improving CW-FG gel properties through the judicious use of oak acorn phenolic extracts at specific concentrations and pH levels, with Ox-OPP showing promising effects on the gel's chemical and functional characteristics.

Both oxidized and unoxidized oak phenolic extract showed high potential for modification of physico-chemical and functional properties of gelatin hydrogel. Also, crosslinking degree was controlled by concentration changes. Formation of higher molecular weight structures and hydrogen bonds between gelatin and phenolic compounds were determined as important factors for the observed results. These improved physicochemical properties of gelatin could lead to the development of products in the food industry that meet consumer demands.

Breast cancer is the second leading cause of cancer-related death among women in the world. To date, chemotherapy has been the most common treatment for breast cancer. However, some natural compounds have been used as chemical alternative treatments for breast cancer due to their wide range of bioactive properties and low toxicity in human and animal models. Curcumin is a natural polyphenol with anti oxidant, anti-inflammatory, anti-microbial and anti-cancer properties. The therapeutic capability of Curcumin is remarkably restricted by its low solubility in water and low bioavailability. Therefore, it needs suitable carriers. Mastic gum is a natural resin obtained from broad-leaved variety of Pistacia lentiscus. The purpose of this research is to investigate the morphological characteristics, zeta potential, particle size distribution, release mode in the simulated oral environment, and the effects of modified Mastic gum microcapsule containing curcumin on MCF-7 cell line.

This study was performed by an in vitro assay and the anticancer effects of Mastic gum microcapsule containing Curcumin were determined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide). The surface morphology, zeta potential and particle size distribution of modified Mastic gum microcapsule containing curcumin was investigated by FE-SEM, Zetasizer respectively.

The images obtained from the scanning electron microscope showed that the modified Mastic Gum Microcapsule containing Curcumin had a smooth surface, without pores and a spherical appearance. The microcapsule particle size was in the range of 2093-5050 nm, the average size of the particles (equivalent radius) was 3093 nm. Zeta potential was measured -4.66±0.2(mv). Correlation coefficient in the Korsmeyer-Peppas mathematical model to evaluate the release pattern of curcumin from the modified Mastic Gum Microcapsule containing Curcumin had the highest value. Furthermore, results of MTT analysis demonstrated that modified Mastic gum microcapsule containing Curcumin had more cytotoxic effect (reduction effect) on breast cancer MCF-7 cell line, after 48 and 72 h of incubation compare free Curcumin in same dosage. The IC50 of modified Mastic gum microcapsule containing Curcumin was found to be 3.830 µg ml-1 after 48 h of incubation, while that of free Curcumin was calculated to be about 6.460 µg ml-1. The IC50 of modified Mastic Gum microcapsule containing Curcumin after 72 h incubation was found to be 2.014 µg ml-1, while that of free Curcumin calculated to be about 4.528 µg ml-1.

Our findings indicated that modified Mastic gum microcapsule containing curcumin had significan reduction effect on human breast cancer cell MCF-7 cell line in compare free Curcumin. Furthermore, our results showed that the surface morphology, zeta potential and particle size distribution of modified Mastic gum microcapsule containing Curcumin were suitable for using in food and drug product basis mouth releasing.

The seeds of Cucurbitaceae plants (pumpkin, melon, etc.) are one of the rich sources of protein. Proteins are one of the most important nutritional sources for humans. Enzymatic hydrolysis of pumpkin seed protein by using microwave pretreatment leads to the production of hydrolyzates with bioactive properties, including antioxidant activity. The use of microwave pretreatment causes changes in the 3D structure of proteins, It opens the 3-dimensional structure of the protein and accelerates the access of the enzyme to the peptide bonds. Therefore, the use of microwave pretreatment is a suitable method to save time and enzyme concentration which are used in enzymatic hydrolysis. In this study, The solution of pumpkin seed protein concentrate was exposed to microwave energy with a power of 500-900 W for 30-90 seconds and it was used as a substrate solution in enzymatic hydrolysis experiments. Enzymatic hydrolysis by pancreatin, with the concentration of 0.5 to 2.5% compared to the protein substrate, was performed in the time from 20 to 190 minutes, at the optimum temperature and pH of pancreatin, in order to produce hydrolysates with antioxidant potential. Antioxidant power was measured by using DPPH radical scavenging activity methods, total antioxidant activity (Absorbance at 695 nm) and iron chelating activity. The highest amount of antioxidant activity by using microwave pretreatment was in 105 minutes and the ratio of 1.5% E/S and the optimal conditions that provided by the software to achieve the maximum iron chelating activity (95.5%), DPPH radical scavenging activity (51.5%) and total antioxidant (Absorbance at 695 nm) (0.976), was in 102 minutes and the ratio of 1.5% E/S of which 89.5% corresponded with the obtained results.

In recent years, due to concerns about the safety and health of synthetic antioxidants, there has been widespread attention to the use of natural antioxidants. Bioactive peptides are one of these types of natural antioxidants. Turkmen melon seeds are a rich source of protein. The purpose of this study, was to perform enzymatic hydrolysis of Turkmen melon seed protein with pancreatin and alcalase enzymes in the ratio of enzyme to substrate of 1% and at intervals of 20-200 minutes. The degree of hydrolysis and antioxidant properties (DPPH radical scavengingactivity, Fe3+ reducing power, and total antioxidant activity) of the obtained hydrolysates were investigated. Based on the results obtained from the effect of time on hydrolysis for alcalase-derived hydrolysates, the treatment was 20 to 120 minutes longer than pancreatin, while the treatment of pancreatin-derived hydrolysates for 140 to 200 minutes was higher than that of alcalase. The results showed that there was no significant difference between most of the hydrolysates from pancreatin and alcalase at 180 minutes compared to 200 minutes (about the free radical scavenging activity of DPPH hydrolyzes from alcalase, 160 minutes was determined as the best treatment). The highest scavenging activity of DPPH was total antioxidant by pancreatin enzyme after 160 minutes and 180 minutes of hydrolysis and respectively 72.49% and 1.27 (absorption at 659 nm). The results showed that the produced hydrolysates have a high ability to prevent food oxidation or be used as a medicinal compound.

Probiotic and antifungal properties of the yeasts isolated from fermented pseudo cereals have been rarely studied, yet. In the present study, after isolation of predominant yeasts isolated from fermented black rice, the yeast with the highest survival in simulated gastrointestinal conditions was selected for further studies. Then, the selected isolate was identified by PCR, and subsequently, its probiotic properties including antibacterial effects, antibiotic and antimycotic susceptibilities, aggregation, hydrophobicity and hemolytic potentials were also studied. Antifungal activity of the selected yeast was investigated against some of the foodborne fungi. Sequencing of the PCR products led to the identification of Rhodotorula mucilaginosa as the selected yeast isolate. The isolate maintained 84.39% of its survival in continues acid and bile treatment. Also, the inhibitory effect of the yeast isolate on Salmonella enterica was significantly (p<0.05) higher than the other foodborne bacteria. Yeast isolate had proper aggregation and hydrophobicity capabilities, and it was resistant to the antibiotics tested. Furthermore, the most inhibition of the yeast growth was observed in the presence of ketoconazole, while the selected yeast was resistant to propionate, sorbate, itroconazole and fluconazole. The studied yeast isolate had no hemolytic activity. The antifungal effect of the yeast isolate against Aspergillus flavus was also verified. In accordance with these results, the selected yeast isolate has a proper potential to use as a probiotic or protective culture in production of fermented foods.

There has been an increasing demand for functional analog meat products due to environmental, human health, and animal welfare concerns. Burger analogs are plant-based products that are designed to mimic the taste, texture, and appearance of meat burgers. They are typically made from a combination of plant-based ingredients such as textured vegetable protein, legumes, grains, and vegetables. The goal of burger analogs is to provide a meat-like experience without using animal-based products.These formulations aim to provide a healthier and sustainable alternative to conventional meat products. The organoleptic properties of burger analogs, including texture, taste, and aroma, are crucial for their acceptance by consumers.Prickly pear (Opuntia stricta) is a fruit from the Cactaceae family that contains various beneficial components, including natural pigments, proteins, fibers, and polysaccharides. Pectic polysaccharides and arabinogalactans are two types of polysaccharides found in prickly pear that have thickening properties and can improve the texture of food products. Moreover, Prickly pear is a nutritious and functional fruit that can provide various benefits when incorporated into the diet or used as an ingredient in food products. To date, no health benefit analog burgers incorporating Opuntia fruit have been developed.This study investigated the effects of adding Prickly pear pulp powder at levels of 0.5-2.5% on the physicochemical, sensory, and textural properties of analog burgers.

Analog burgers were formulated according to Iranian national standards using common ingredients (texturized soy protein, water, canola oil, garlic, dehydrated onion, soy sauce, and guar gum) as a control sample. Mature prickly pear fruits (Opuntia stricta) were collected from west of Mazandaran province in February. The  fruits were washed, peeled, and dried in a forced oven dryer at a temperature of 45 °C. The dried samples were then ground into a powder and stored at 4 °C until further physico-chemical parameters of the  including moisture, pH, ash, protein, lipid, color and total phenolic content. For developing new formulation of analog burgers, the roasted flour was substituted with prickly pear pulp powder at 0.5%, 1.5%, and 2.5% of the base recipe. The average moisture, ash, fat, carbohydrate content, pH, holding capacity, and color of each raw packed burger were measured. The hardness, springiness, cohesiveness, and chewiness of cooked analog burgers were evaluated using a texturometer instrument. Sensory analysis was performed by 10 panelists who judged discrimination scales of color, odor, taste, and texture characteristics. Analysis and sample treatments were repeated at least three times. Statistical analysis was performed using SPSS (version 19.0), and data were expressed as means ± standard deviation (SD).

The lowest and highest cooking losses were observed in analog burgers with 2.5% pulp powder (21.03 ±0.47%) and the control (22.2 ± 0.63%), respectively. However, moisture retention and juiciness did not show significant differences (p > 0.05) between analog burgers with prickly pear pulp powder and the control. The results indicated that increasing prickly pear pulp powder levels significantly decreased the redness (+a*) parameter and yellowness (+b*) of raw analog burgers. Moreover, a reduction in cooking loss and shrinkage were observed for cooked soy burger samples using prickly pear pulp powder. However, cooked analog burgers with added prickly pear pulp powder showed significantly higher juiciness. The elasticity of the produced analog burgers significantly decreased with an increasing percentage of pulp powder (p <0.05).

The incorporation of prickly pear pulp powder in analog burger formulation resulted in a significant decrease in cooking loss and shrinkage of the cooked burgers, while not significantly affecting moisture retention and juiciness. An increase in prickly pear pulp powder levels in analog burger formulation led to a significant decrease in the redness and yellowness of the raw analog burgers, as well as a decrease in their elasticity. Based on the sensory evaluation and consumers' overall tendency to consume burgers, it is recommended to use 1.5% prickly pear pulp powder in analog burger formulation.

 High levels of free radicals can damage biomolecules and eventually cause oxidative stress. Bioactive peptides produced during enzymatic hydrolysis keep high health properties, such as antioxidant properties. The production of antioxidant peptides has received much attention as a new generation of natural antioxidants. Plants are one of the most abundant sources of biopolymers, especially protein. As long as the protein structure is intact, its amino acid sequence is inactive; however, during proteolysis, fermentation, and gastrointestinal digestion, these amino acids are released as oligopeptides ordinally with less than 20 amino acids and below 10 kDa in molecular weight. These peptides are more digestible and can exhibit specific bioactive properties such antioxidant properties. In this regard, the use of food waste containing protein to produce bioactive peptides and increase their value has received increasing attention. Enzymatic hydrolysis can increase their functional properties by converting proteins into peptides without affecting their nutritional value. Pomegranate seed protein is a by-product of the pomegranate seed oil industry and can be a good source of bioactive peptides with antioxidant properties. According to our knowledge, there isn’t any data about the enzymatic hydrolysis of pomegranate seed protein for antioxidant peptides production. In this study, the optimal conditions for enzymatic hydrolysis of pomegranate seed protein with trypsin using the responses surface method and the effect of hydrolysis on protein structure were investigated.

 In this study, the protein was extracted from pomegranate seed, and using trypsin the optimization of enzymatic hydrolysis conditions of protein was determined by Face-Centered Central Composite design, which is one of the responses surface design methods. The effect of independent variables including temperature (30 to 45 °C), time (30 to 180 minutes), and enzyme to substrate ratio (1 to 3 w/w) on DPPH free radical scavenging activity and Fe+3 reducing power as responses, was evaluated. Validation tests were performed for confirmation of the proposed values by software and the degree of hydrolysis of the samples was determined. In the next step, the unhydrolyzed and hydrolyzed protein was evaluated for molecular weight distribution and their surface hydrophobicity was compared. Finally, scanning electron microscopy images were used to confirm the hydrolysis process.

 Under optimal conditions obtained from the response surface method (temperature: 37.6 °C, time: 136.55 minutes, and enzyme to substrate ratio: 2.2%), trypsin-derived hydrolyzate, showed DPPH free radical scavenging power: 87±0.89% and Fe+3 reduction power: 0.293±0.44. Under these conditions, the degree of hydrolysis was equal to 30.1±1%. The optimum conditions of hydrolysis were validated by RSM. The increase in the surface hydrophobicity of the protein after the hydrolysis process indicated the unfolding of the pomegranate seed protein chain and the exposure of its structure during the reaction. The electrophoretic profile of denatured pomegranate seed protein showed smaller peptide bands and lower band intensity, along with losing some of the peptide fractions after hydrolysis. so the efficacy of trypsin at cleaving the protein was confirmed. Evaluation of images obtained by scanning electron microscopy showed that unhydrolyzed protein had complex structures comprised of random sheets of different sizes and shapes and the protein degraded into small fragments and looser structure with many folds after enzyme hydrolysis, resulting in smaller particles compared with untreated samples with the same SEM parameters

Considering the consumer’s tendency toward functional foods and present concerns about the application of synthetic additives and according to the results, the hydrolyzed pomegranate seed protein prepared by trypsin shows good antioxidant capacity. In addition, there will be a reduction in waste generated by the pomegranate processing industry. Further studies will need for the isolation and identification of the specific peptides and amino acid sequences and the evaluation of their possible incorporation in food matrices.

Enzymatic hydrolysis of protein sources is a common method in food processing. Hydrolyzed protein is a mixture of peptides and amino acids that have many health properties including anti-hypertensive, anti-cholesterol and antioxidant. Turkmen melon seed is one of the fruit by-product which can be a cost-effective source for the production of bioactive peptides. In this study, enzymatic hydrolysis of Turkmen melon seed protein with pancreatin and trypsin enzymes in enzyme to substrate ratio of 1% and time intervals of 20-200 minutes was performed. The degree of hydrolysis and antioxidant properties (DPPH radical scavenging activity, Fe3+ reducing power, total antioxidant activity and hydroxyl radical scavenging activity) of the obtained hydrolysates were investigated. The results showed that with increasing the hydrolysis time up to 180 minutes, the degree of hydrolysis increased significantly and after that no significant differences was observed in this factor. The maximum DPPH scavenging activity, Fe3+ reducing power and hydroxyl radical scavenging activity were obtained by hydrolysis with pancreatin enzyme and after 180 minutes of hydrolysis and were 72.49%, 0.55 (adsorption at 700 nm) and 59%, respectively. The maximum total antioxidant activity of pancreatin and trypsin hydrolysates was obtained after hydrolysis at 180 minutes and there was no significant difference between them. According to the results, compared to trypsin, pancreatin enzyme showed a better performance in the production of protein hydrolysate with maximum antioxidant properties. As a result, the resulting hydrolysates, can be used in food formulation as a natural preservative and the production of functional products.

Today, Pickering emulsions, as the most stable type of emulsion, have been widely accepted for use in various food, pharmaceutical, and cosmetic products. The common use of biopolymers such as proteins and polysaccharides as stabilizing or thickening agents in the formulation of these emulsion systems, due to their inherent functional characteristics, easy preparation, nutritional value, biodegradability, and biocompatibility, encourages researchers to design new hybrid Pickering stabilizers with enhanced functional properties. Therefore, in the present study, the fabrication of sodium caseinate/soy soluble polysaccharides conjugate nanoparticles and the potential of these novel emulsifiers to stabilize the Pickering emulsion was investigated.

Materials and methods:

First, sodium caseinate/soy soluble polysaccharides conjugates (NaCS/SSPS) with a protein/polysaccharide ratio of 9 to 1 were produced through the Maillard reaction using the dry heating method under optimal conditions (temperature of 60 oC and 75% relative humidity ), and after confirming the formation of covalent binding between NaCS and SSPS via attenuated total reflectance-Fourier transform infrared spectroscopy, the conjugate capability to create a stable emulsion was studied, and compared to native biopolymers. Then, to produce NaCS/SSPS conjugate nanoparticles (Pickering stabilizer), ultrasonic pulse treatment was applied (400 W- 28 minute) and two important features of particle size and their wettability were measured. In the following, the potential of Pickering emulsion formation using NaCS/SSPS conjugate nanoparticles was characterized and the stability of obtained emulsions was compared to that of untreated NaCS/SSPS conjugates.

The formation of covalent binding between NaCS and SSPS and structural changes of NaCS during conjugation reaction were detected through attenuated total reflectance-Fourier transform infrared spectroscopy. Increased physical stability against creaming was observed for emulsions stabilized by conjugates when compared with those produced by the native biopolymers. In addition, differential scanning calorimetry results showed that the thermal stability of NaCS was significantly increased by glycosylation reaction. Based on the dynamic light scattering and contact angle measurements, the ultrasonic treatment led to a significantly decreased particle size and an increase in their wettability, which allowed the creation of a high stable Pickering emulsion. The enhanced wettability of the NaCS/SSPS nanoparticles was achieved by exposing the hydrophobic sections hidden within the conjugate structure.

Owing to the successful fabrication of high stable Pickering emulsion using the hybrid nanoparticles produced by combining two techniques of conjugation and ultrasonic method, it can be suggested to employ NaCS/SSPS conjugate nanoparticles for the manufacture of Pickering emulsion delivery systems for bioactive compounds.

The aim of this study was to extract and encapsulate the phenolic and flavonoid compounds of wild dill (Grammosciadium Platycarpum Boiss) in sodium alginate hydrogel. In this study, phenol and flavonoid compounds were extracted from Grammosciadium Platycarpum Boiss and then used for encapsulation at three concentrations of 1, 2 and 3%) in sodium alginate with three concentrations of 1.5, 2 and 3%. In the next step, antioxidant activity (total antioxidant, DPPH free radical scavenging capacity, OH radical scavenging, reducing power), loading capacity of phenolic compounds of Grammosciadium Platycarpum Boiss extract in alginate hydrogel, encapsulation efficiency and process efficiency, were evaluated. Also, structural properties of encapsulated material including chemical structure using infrared spectroscopy were measured. The ability to preserve phenolic compounds and antioxidant properties of the product during 30 days of storage in different environmental conditions (light and dark, and temperature 25 ° C and 4-7 °C) were also evaluated. The results showed the maximum loading capacity of 1.178 mg as gallic acid per gram of extract was obtained for alginate hydrogel containing 3% extract. In addition, it was found that by using this method, a high coating efficiency of about 75% was obtained. The results also revealed a significant difference (P<0.05) in the ability to maintain antioxidant activity against environmental conditions, for hydrogels containing different amounts of extract during 30 days of storage. Similar results obtained with respect to the ability of phenolic compounds retention during storage in hydrogels containing the extract, the maximum retention of total antioxidant properties, free radical scavenging of DPPH, free radical scavenging of OH and reducing power under different storage conditions, were observed for hydrogels containing 2% extract. The greatest reduction in antioxidant properties was observed for hydrogels containing 1% extract under artificial light conditions. The ability to maintain the antioxidant property of the was observed in the artificial light condition about 55/18% and in the dark conditions about 74/15%.the rate of free radical DPPH in artificial lighting conditions accounted for about 36/42 %, and about 84/15 % in the dark conditions. The rate of free radical OH in artificial lighting conditions accounted about 44/11% and about 68/34% in the dark conditions. The rate of power regenerative in artificial lighting conditions was 63/53 % and was estimated at 75/83% in the dark conditions. In this study it was found that 2% alginate containing 2% extract has the best encapsulation and has the highest retention of phenolic compounds and antioxidant properties during storage.

Nowadays, replacing gluten is one of the challenging issues in cereal field. Since gluten-free products do not have desirable textural and sensory characteristics, using compounds such as proteins and gums can help to improve the quality of them. Proteins and hydrocolloids can mimic the viscoelastic properties of gluten to some extent. Legumes are important and inexpensive food resources rich in protein and can compensate for the lack of lysine amino acid in cereals. Thus, they are considered as a good food supplement for cereals. Broad bean is one of the legumes rich in protein (25-32% protein) and is considered as a suitable source for the production of protein concentrate. Xanthan gum is also one of the most effective hydrocolloids used in gluten-free products. The aim of this research work is to evaluate the effect of broad bean protein concentrate (BPC) and xanthan gum on physicochemical properties of rice batter and cake.

In the present study, BPC was extracted by acidic-alkaline method and was then added to the batter formulation at three levels of 0, 7.5, 15 % with xanthan gum at three levels of 0, 0.15, 0.3%. Physicochemical properties of the batter including specific gravity and viscosity were evaluated. Physicochemical and sensory properties of cake including porosity, volume, baking loss, hardness and sensory properties were evaluated.

Results of the batter analysis showed that viscosity was reduced by increasing the amount of BPC and xanthan and no significant difference was observed in specific gravity of samples. Results of the cake analysis revealed that hardness was increased and porosity, volume and baking loss were reduced by increasing the amounts of BPC and xanthan. Results of the sensory evaluation showed that there was no significant difference between the treatments in the parameters of crust and crumb color, hardness and porosity; and the panelists assigned lower score to the treatments containing 15% of bean protein concentrate (BPC) regarding the texture and flavor. In terms of total acceptance, the panelists assigned the highest score to the control sample and the sample containing 7.5% broad bean protein concentrate (BPC) and 0.3% xanthan.

In general, high amounts of broad bean protein concentrate (BPC) and xanthan gum led to a significant increase in batter viscosity which resulted in an increase in hardness and a decrease in volume and porosity of the cake by creating high water absorption, but led to a decrease in baking loss of the cakes, which is economically favorable. According to the obtained results, cake with 7.5% BPC and 0.3 % xanthan was the best treatment in terms of sensory and quality characteristics.

Free radicals and hydroperoxides originate from oxidation reactions could reduce food quality and also promote incidence of various diseases such as cancer. In this regard, the use of natural compounds with antioxidant properties, such as bioactive peptides, is of interest to many researchers. Demand for natural and safe antioxidants such as bioactive peptides has increased dramatically due to concerns about the safety and long-term health implications of synthetic antioxidants such as BHT and BHA. Bioactive peptides are defined as hydrolyzed proteins that, after entering the body, show potential ability to stimulate desirable and health promoting activities.

The aim of this study was to optimize the hydrolysis conditions of edible mushroom (Agaricus bisporus) powder protein with alcalase enzyme in order to produce hydrolyzed protein with potential antioxidant properties. To conduct this research, first, edible mushrooms were turned into powder after purchase from the market and conducting related processes. Then, in order to optimize the production of hydrolyzed proteins with maximum antioxidant activity using response surface methodology, the independent variables selected as time, temperature and ratio of enzyme to substrate and dependent variables was selected as the antioxidant capacity of the hydrolyzed sample (using DPPH free radical scavenging methods, iron ion reduction power and total antioxidant capacity). The temperatures used were in the range of 40-55 °C, the hydrolysis time between 30 to 210 minutes and the enzyme to substrate ratio 1 to 3%, which was optimized by the surface response methodology. In this research, Design Expert statistical software was used to apply the response level of statistical design. The statistical design was prepared with a central composite design with 20 experimental treatments that included a central point with six replications.

The obtained results showed that all the studied parameters (enzyme concentration, temperature and time of hydrolysis) had a significant effect on the antioxidant activity of the hydrolyzed product. The results showed that for the production of hydrolyzed protein of edible mushroom with high antioxidant activity (DPPH free radical scavenging methods, reducing power of Fe3+ and total antioxidant capacity) by the alcalase enzyme, the optimal conditions include temperature of 47.5 ° C, the hydrolysis time of 197 minutes and the enzyme-to-substrate ratio 2.1%, which with desirability of 100% was equal to DPPH free radical scavenging of 10.661%, reducing power of Fe3+ equal to 2.45 (absorption at 700 nm) and the total antioxidant capacity of 1.224 (absorption at 695 nm).

The results showed that by hydrolysis of protein of edible mushroom under optimal conditions a product with suitable antioxidant capacity can be obtained, which can be used as natural and functional additives in food formulations.

Tussilago farfara L. is a perennial forb belonging to the Asteraceae family. The leaves and flowers of this plant have medicinal properties, and its essential oil has been used for the treatment of dry coughs and inflammation of the mouth, throat, and respiratory tract. This study aimed to assess some phytochemical characteristics of Tussilago farfara L. and compare changes in this plant at different altitudes.

To investigate the changes in the amount of the effective substance of the studied plant in the altitude gradient, altitude classes of 1100-2600 meters were considered in Golestan province, Iran, considering the presence and abundance of the species. In each phenological stage (before and after flowering time), plant samples were randomly collected in each altitude class and transferred to the laboratory. Total phenol content and flavonoid content were measured using the Folin-Ciocalteu and aluminum chloride methods, respectively. The antioxidant activity of the methanolic extract was also evaluated using the DPPH (2,2-Diphenyl-1-picrylhydrazyl) reagent.

The results showed that the highest amounts of total phenol content, flavonoids, and antioxidant activity were observed at altitudes above 2200 meters, and there was a significant difference in plants (Tussilago farfara L.) grown in different altitudes in terms of phytochemical properties. Based on the comparison of phytochemical properties of this plant in different phenological stages, the highest content of total phenol and flavonoids were observed in the phonological stage of flowering and the highest (42.08%) antioxidant activity of leaves was observed in the vegetative stage.

The results of this study can help activists in the field of medicinal plants to determine the appropriate height range and phenological stage in harvesting the leaves of this plant to achieve the highest levels of phenol content, flavonoids, and antioxidant activity.

Dairy Spreads are solid and spreadable food products that merely made from milk or milk products that contain 10 to 80% milk fat. samanou is a traditional Iranian dessert that is prepared by cooking a mixture of wheat flour and green malt extract. The aim of this study was to assessment the effect of adding xanthan gum (0, 0.5 and 1%) and chia seed (0, 0.5 and 1%) on physical stability and textural and rheological properties dairy spread containing samanou powder.

In the present study, the frequency sweep rheological test was performed by a rheometer. During this test, first the linear viscoelastic range is determined and then in the frequency range of 0.1 to 100 Hz, temperature of 10 ° C and constant strain of 1%, the rheological properties of the spread including storage modulus (G′), loss modulus (G′′) and loss tangent (G′′ /G′) were measured. Emulsion stability was measured using a centrifuge based on oil separation (g). texture evaluation was performed by backward extrusion and spreadability tests with a texture analyzer to determine of firmness (N), cohesievness (N), consistency (Ns) and spreadability (N) parameters.

The results showed that the effect of adding xanthan gum and chia seed flour on increasing the cohesievness, consistency, firmness and reducing the spreadability of the produced spread was significant (p<0.05). Increasing the concentration of xanthan and chia seed flour from zero to 1% increased the cohesiveness and firmness and also redused the spreadability of the produced spread compared to the control sample. The treatment containing 1% xanthan and 1% chia seed flour had the highest firmness and the least spreadability, respectively. spreads containing xanthan were more consistency than spreads containing chia seed flour, so the highest and lowest consistency were in the treatment containing 1% xanthan and without chia seed flour (67.53±12.66 N.s) and the treatment prepared from 1% Chia seeds flour without xanthan of (37.36±17.5 Ns), respectively. The lowest and highest cohesievness were in the treatment containing 0.5% xanthan (-0.05±0.22 N) and the treatment containing 1% xanthan and 0.5% chia seed flour with a score of -0.31±0.39 N respectively. Spreads containing xanthan gum and chia seed flour were more stable than the control sample, so that the control sample had the highest oil separation and the lowest physical stability (0.476±0.13 gr). In frequency sweep test, 1% strain was determined as the best strain corresponding to the linear viscoelastic range. by simultaneous increasing the concentration of xanthan and chia seed flour, the amount G′, G′′ and G′′ /G′ decreased. The presence of chia seed flour in the spread formulation, unlike xanthan gum, increased the amount of G′ and G′′. The maximum amount of G′ and G′′, as a result of the highest elasticity, , consistency and , firmness belonged to the treatment containing 0.5% xanthan and the treatment containing 1% xanthan and 0.5% chia had the lowest amount of G′ and G′′ Dedicated to himself.

application minimum concentrations of xanthan (0.5%) and chia seed flour (0.5%) improved the textural properties of dairy spreads containing samanou powder. Increasing the concentration of chia seed flour, unlike xanthan, weakened the internal bonds and reduced the consistency of the product. The presence of xanthan compared to chia seed flour had a greater effect on preventing phase separation and increasing the stability of dairy spread containing of samanou powder. In all frequency ranges, the amount of storage modulus was greater than the drop modulus, and the spreads produced behaved like a gel viscoelastic solid.

Characterization of lactic acid bacteria (LAB) isolated from rarely studied fermentation substrates can lead to isolate unique microorganisms. In the present study, predominant LAB was isolated from fermented acorn using repeat of back-slopping process, and then the isolate was identified by PCR. Subsequently, probiotic properties of the isolate (including resistance to acid and bile, antibacterial, auto and co-aggregations, antibiotic susceptibility and blood hemolysis), as well as its antifungal effect were studied. Sequencing results of the PCR products led to the identification of Pediococcus acidilactis as predominant LAB isolate. The survival percentage of the isolate in continuous acid and bile treatment was equal to 72%. The inhibitory effect of the isolate on Bacillus cereus was also significantly (P<0.05) higher than the other foodborne indicator bacteria. Furthermore, crude cell free supernatant (CFS) obtained from LAB culture completely inhibit the growth of B. cereus; meanwhile, its naturalized CFS had no inhibitory effect on Salmonella enterica. LAB isolate had also proper auto and co-aggregation (with E. coli) potentials and had no hemolytic activity. Antifungal activity of the isolate against Aspergillus niger was also verified. By considering the proper probiotic and antifungal potentials of the P. acidilactis isolated from fermented acorn it is possible to use the isolate as microbial starter, adjunct, probiotic and or protective culture in fermentation industries.

In this study, montmorillonite (MMT) was modified by hexadecyltrimethyl ammonium bromide (HDTMA) to enhance its adsorption capacity and improve its functional properties that it was done through conventional ion exchange. The intercalation of cationic surfactant into layers of MMT and properties of MMT before and after modification was evaluated by Fourier transform infrared (FTIR), X-ray diffraction (XRD) spectroscopy, Thermal gravimetric analysis, contact angle, and zeta potential. The XRD results showed the interlayer spacing was increased from 1.17 to 2.02 nm due to intercalation of HDTMA. The contact angle and zeta potential analyses revealed that the surface wettability of MMT was changed from hydrophilic to hydrophobic and the surface charge was changed from -21.6 to -2.54 due to adsorption of HDTAM on surface of MMT. FTIR spectra show the successful of inserting alkyl groups from cationic surfactant in the interlayer space of MMT. The results are supported by the measurements of TGA/DTA. The findings of this study are useful for the application of modified MMT as a nanoadsorbent for food, chemical, and pharmaceutical industries.

Fine wheat powder is a natural polymer that obtained from of pneumatic process in which light particles are not allowed to be used in bakeries despite the presence of nutrients and lead to reduced quality of flour. The purpose of this study was production of emulsion film based on wheat soft powder as a new and inexpensive raw material and investigation of two types lipids (oleic acid and beeswax) at different concentrations (5, 10 and 15%) on physical and mechanical properties of emulsion films. The results showed that by increasing lipid concentration, thickness, turbidity and tensile strength increased. But moisture decreased and elongation at break decreased as compared the control film. Comparison between the lipid concentration showed that increasing the concentration of beeswax compared to oleic acid had more effect on increasing the turbidity value of the emulsion film. Water vapor permeability of emulsion film containing 5% oleic acid (1.82×10-10 g-1m-1s-1pa-1) was lower as compared beeswax film at similar concentrations (2.30×10-10 g-1m-1s-1pa-1). In addition, scanning electron microscopy images showed the surface of 10% oleic acid film was smooth and uniform with smaller pores as compared the control sample which confirm the water vapor permeability results. The results of this study indicated the good capability of fine wheat powder in preparation of emulsion film containing oleic acid and beeswax application in food packaging.

Rice is one of the most suitable cereals for the production of gluten free products for patients with celiac disease and most gluten-free products are prepared based on rice. Rice has unique nutritional properties, contains low sodium, protein, fat and high amounts of digestible carbohydrates, But since in the milling process, in order to produce white rice, a significant portion of fiber and minerals are lost, in the production of gluten-free products from rice flours, we must use of other cereals flour or authorized additives in order to achieve the desired nutrient in terms of nutrients and provide the needs of patients with celiac disease. Millet is rich in dietary fiber, protein, minerals and vitamins and can meet the needs of patients with celiac disease. Xanthan gum is also one of the most commonly used additives in the production of gluten free products, and helps to improve the quality and physicochemical properties of gluten-free cakes. The purpose of this research is to replace rice flour using millet flour combined with xanthan gum in the production of gluten-free cake to provide a new formulation.

In the present study, gluten-free cake was prepared based on rice flour, replacing 50, 75 and 100% rice flour with millet flour and using 0.15 and 0.3% of xanthan gum.Then, physicochemical and sensory properties of gluten-free batter and cake including viscosity, cooking loss, specific volume, porosity, texture, moisture content and total acceptance were evaluated.

The results showed that the amount of millet flour increased, the viscosity of the dough, moisture content, porosity and softness of the cake texture decreased but these properties increased with the addition of xanthan gum. But these properties increased with the addition of xanthan gum. It was also found that combining the appropriate amount of millet and xanthan gum with proper viscosity can improve the physicochemical and sensory properties of the dough and cake. By adding 50% millet flour and 0.15% xanthan gum, the physicochemical properties of cake were improved, but by increasing this amount (75 and 100% millet flour and 0.3% xanthan gum), the cake quality was reduced. The sample containing 50% millet flour and 50% rice flour with 0.15% xanthan gum had the highest volume, porosity and sensory acceptance, as well as the least firmness during the storage period.

According to the results, using 50% millet flour in combination with 0.15% Xanthan gum could improve the physico-chemical and sensory properties of the dough and cake to the control sample (100% rice flour).

The formation of chitosan nanoparticles (CSNPs) with a high stability still remains a main challenge in terms of applying the produced particles in the field of nutraceutical and drug delivery systems. Giving that there are many variables parameters which could affect the size, morphology, and other properties of fabricated CSNPs during ionic gelation process along with using sodium tripolyphosphate (STPP) as the most common cross-linking agent. In this study, after the production of CSNPs under the influence of various independent variables such as chitosan (CS) concentration, STPP concentration, and CS to STPP ratio, in the next step, the physical, rheological, turbidity, and colorimetric properties of the produced nanoparticles were measured. Finally, two artificial neural networks (ANNs) – multilayer perceptron (MLP) and radial basis function (RBF) – with a single hidden layer and different threshold functions, learning algorithms, etc. were employed to predict the CSNPs properties. The results revealed that MLP for the physical, viscosity, b*, and chroma properties and RBF for other properties – with a Levenberg-Marquardt (LM) learning algorithm of 1000 epochs – well predict them with a very high determination coefficients (R2) and low mean square error (MSE). R2 for nanoparticle size, poly dispersity index (PDI), zeta potential, viscosity, and electrical conductivity of CSNPs suspensions were determined 0.9881, 0.9534, 0.9431, 0.9212, and 0.9636, respectively. However, RBF with a single hidden layer comprising a set of 3 inputs, 4 neurons in hidden layer, and 3 outputs with the SigmoidAxon- SigmoidAxon transfer function presented the best results for predicting the L*, ΔE, and WI properties of CSNPs suspensions. In addition, R2 for L*, ΔE, and WI of CSNPs were calculated 0.9586, 0.9775, and 0.9457, respectively. Also, the flow behavior index of CSNPs suspensions was determined less than 1, which indicates the pseudoplastic behavior of the samples.

Today, the most common way to increase the quality of gluten-free products is to use gum. According to recent studies, physical modification techniques such as microwave treatment can be used to improve the functional properties of flour and gluten-free baking products.

Therefore, in order to compare the effect of xanthan gum and microwave treatment of millet grain on physicochemical and sensory properties of gluten cake (obtained from millet and rice flour with 50:50), xanthan gum was used at two different levels of 0 and 0.15% and microwave treatment was applied at three levels of moisture 10, 15 and 20% and times 60, 30 and 90 s with a constant microwave power of 900 W.

According to the results, the use of the microwave treatment of millet grain reduced the specific gravity and increased viscosity of gluten-free cake batter (p < 0.05). The cake samples prepared from the microwave treated grain with 20% moisture content at 90 or 60 s showed the highest volume, porosity, and sensory acceptance. Moreover the softest texture and the highest moisture content during storage belonged to the sample preparedwith flour from the microwave treated grain under 20% and 90s.

According to the results of this study, microwave treatment of millet grain could be a good alternative to using gum in a gluten-free cake.