فهرست مطالب j.keramat

In the past decade, sulfated fucose-rich polysaccharides (fucoidan) have been much interested due to their diverse biological activities, including anticoagulant, antiviral, antitumor, anti-inflammatory, anti-blood lipid, antioxidant, gastric protection and therapeutic activities. In the present study, microwave was used as a green efficient technology for the extraction of bioactive compounds of fucoidan from Iranian macroalgae (Nizimuddinia zanardini). In addition, effects of microwave on chemical composition and antioxidant activity of fucoidan were assessed.

Fucoidan polysaccharides were extracted under optimized conditions of microwave power, processing time and temperature. The compound characteristics, including extraction efficiency, fucose content, sulfate content, uronic acid content and DPPH radical scavenging were assessed. Fourier-transform infrared spectroscopy was used at wavelengths of 400–4000 cm-1 at room temperature.

Results showed that microwave treatment was effective in improving the extraction yield of fucoidan. Fucoidan extraction yields under the microwave technique included 3.71 and 2.35%. The fucose content under the microwave technique (33.17%) was compared to that under the conventional technique (29.5%) with significant increases, indicating less thermal degradation of the polysaccharide structures under the microwave technique. The microwave improved fucoidan antioxidant activity by producing shorter chain polysaccharides.

In this study, fucoidan sulfated polysaccharides were extracted using microwave extraction technique in a much shorter time than the conventional extraction technique with higher efficiency and lower thermal degradation. In addition, the shorter process time decreased solvent use.

This study aimed at investigating the effects of drying temperature, heat treatment and gum concentration on emulsifying properties of Farsi gum, extracted from Amygdalus scoparia Spach. For this purpose, solutions of 3, 3.5, 4 and 5% concentration of Farsi gum were prepared. These solutions were heat treated at 65, 75 and 85 °C for 1 minute. Then, they were dried by spray dryer at 120,150, 165 and 180 °C. The 20% sunflower oil emulsions were prepared at 0.5, 1, 1.5, 2 and 3% concentration of Farsi gum. The results showed that a decrease in drying temperature made improvement in the emulsion ability and emulsion stability of Farsi gum; but heat treatment and gum concentration had no significant effect (p < 0.05) on the emulsifying properties of Farsi gum. Emulsions contained 1% Farsi gum were stabled after a week and creaming was not observed, also after a month, emulsion contained 2% Farsi gum was stabled. By increasing the drying temperature, creaming index decreased but gum concentration and heat treatment had no significant effect (p < 0.05) on creaming index. The average size of droplets of emulsion decreased with increasing concentrations of Farsi gum from 0.5% to 1.5%-2% but with increasing concentrations of Farsi gum to 3%, the average size of droplets of emulsion increased. Among the factors involved, only drying temperature had significant effect (p < 0.05) on size of droplets of the emulsions. Consequently, the average size of droplets increased with increasing drying temperature.

Macroalgae are source of many polysaccharides. Based on their pigments content they are divided into brown, green and red algae having specific polysaccharide contents. Among them, brown algae (Phaeophyceae) are source of alginates and/or fucans or fucoidans (Percival and McDowell 1990, Torres et al., 2007). Alginates are anionic polysaccharide that existences in mixer of salt forms (Na+, Mg2+ and Ca2+) in cell walls of brown algae makes the tissues flexible and strong. They consist of (1,4) linked β-d-mannuronic acid (M) with 4C1 ring conformation and a-l-guluronic acid (G) with 1C4 ring conformation, the two uronic acids being in pyranosic conformation(Pawar and Edgar 2012, De Sousa et al., 2007). They offer three varying sequences identified as the blocks MG consisting of nearly equal proportion of both monomers with a high number of MG (or GM) dimmers, the blocks GG and the blocks MM (Haug and Larsen 1962). M/G ratio, length and distribution of sequences depend on the algae species but also on their growth conditions and geographic origins. It also determines the chemical and physical properties of alginates (Karaki et al., 2013). Indeed, their gelling properties are dependent on guluronic acid content and explained by the structural features of GG blocks where selective alkaline earth metal multivalent cations and notably Ca2+ take place by chelation. This phenomenon is known and explained as the “egg-box” model. The formed gel is not soluble in water. However, sodium alginate is a water-soluble polymer having generally pseudo plastic properties in solution (Draget and Taylor 2011). Polysaccharides have wide range of pharmacological activities and some of them are considered as antitumor, antihypertensive, immunomodulator as well as antioxidant agents. Their radical scavenger’s role can be used to inhibit oxidative damage in foods and improve their nutritional quality (Venugopal 2011, Yang et al., 2011).

Twenty-five gram of dried algae (Nizamuddinia zanardinii) was soaked under steering at room temperature for 24 h in 800 mL of 2% (v/v) formaldehyde to remove phenolic compounds and pigments. After that, the insoluble fraction was washed 3 times with MilliQ water and supplemented by 800 mL of 0.2 M HCL before to be incubated at different levels(25-60°C) for different extraction time periods(0.5-3 h) under stirring (250 rpm).After optimizing of acidic step, the suspension was then centrifuged (10000 g for 20 min at 20°C) and pellets with alginic acid were washed 3 times with MilliQ water before to be socked in a range from (2-4 % (w/v)) Na2CO3 solution for different levels (2-4 h) at different extraction time periods (60-90 °C). The mixture was centrifuged (10000 g for 30 min at 20°C) and the supernatant was precipitated with 3 volumes of ethanol 96 % (v/v). Pellets containing sodium alginate were dissolved in MilliQ water and precipitated with ethanol as described above. This step was repeated two times before to collect and freeze dried the sodium alginate. Response surface methodology (RSM) was used to study the effect of extraction temperature; X1 extraction time; X2 for acidic step and concentration; X1 , extraction time; X2,; extraction temperature X3 for alkaline step. A D-optimal design was employed for designing experimental data using Design-Expert 10.0.3 trial software (Stat-Ease Inc., Minneapolis, MN, USA).

Both temperature and time factors have a significant effect on the extraction efficiency and the effect of temperature on extraction efficiency is higher than the effect of time in acidic step. There is also a significant interaction between the two variables of temperature and time. The extraction temperature had an increasing effect on alginate extraction efficiency. Chloridric acid disrupts the cell wall and releases alginate and also converts alginate salts in the cell wall into arginic acid. Increasing temperature causes further cellular breakdown and alginate extraction efficiency increases. With increasing time, the extraction efficiency increased. This increase can be due to the fact that the acid needs time to penetrate the cell wall, and as the time increases, the permeability increases, resulting in the extraction efficiency, so that the permeability is complete and the highest efficiency is obtained and After that, the extraction efficiency remains constant and even due to the long-lasting effect of acid, it causes de-polymerization and decreases the extraction efficiency. Increasing temperature increased the effect of time factor, meaning that the interaction of temperature and time had a significant effect on extraction efficiency. At low temperatures, the effect of time on the extraction efficiency is low because of the breakdown of the cell wall, but at higher temperatures, which contribute to the thermal breakdown of the cell wall, the effect of time variation is more evident. In alkaline step the effect of concentration on extraction efficiency was considered as a quadratic equation and as interaction with extraction time. By increasing the concentration of sodium carbonate, the extraction efficiency increases with an ascending slope. In other words, the amount of efficiency increases with an increase in direct concentration. Sodium carbonate causes the sodium substitution in carboxylic acid arginine groups and sodium alginate salts are obtained. Therefore, increasing concentration results in more calcium ion replacement and alginate extraction efficiency increases. This increase in extraction efficiency is increased to the extent that all carboxylic groups are replaced by sodium ion, and after that the extraction efficiency remains constant, and even due to the high concentration and de-polymerization of the alginate, it reduces the extraction efficiency. With increasing time of extraction with sodium carbonate, the extraction efficiency decreases with a second order gradient. Sodium ion has been completely replaced in argonic acid groups during the initial extraction hours, and if the extraction time is increased due to the prolonged contact, sodium alginate is obtained with an excess of sodium carbonate which has an alkaline pH and because of the adverse effect of this pH Extraction efficiency is reduced. The effect of temperature on extraction efficiency was considered as a first-order equation and as interaction with extraction time. As the temperature rises due to breaking of the bonds that may lead to the separation of sodium ions and also cause de-polymerization of the alginate, the extraction efficiency decreases.

Chloridric acid disrupts the cell wall and releases alginate and also converts alginate salts in the cell wall into alginic acid. the acid needs time to penetrate the cell wall. The results showed that in acidic step temperature and time are two major effective factors on yield and quality of extracted alginate. Sodium carbonate causes the sodium substitution in carboxylic acid arginine groups and sodium alginate salts are obtained. Sodium ion has been completely replaced in argonic acid groups during the initial extraction hours, in high temperature may lead to the separation of sodium ions and also cause de-polymerization of the alginate so in alkaline step, all factors have effect on yield, polyphenol and apparent viscosity of extracted polymer. The optimized conditions of extraction of sodium alginate from brown algae (Nizimuddinia zanardini)were as follows: temperature: 60 oC, time: 3 h (acidic step) and temperature: 60 oC, time: 3 h and concentration 3 % w/v (alkaline step).

In this research, the content of glycerol were investigated in two amphypod species of Gammarus pseudosyriacus and Gammarus bakhteyaricus from Khersonak and kalicheh during different seasons for one-year. The species were collected seasonally from the beginning of autumn 2015 to summer 2016 from the sampeling stations. The samples were dried, extracted and then injected into high performance liquid chromatography (HPLC) for determination of trehalose and glycerol content. The glycerol contents showed significant difference (P<0.05) among different seasons. In spring and autumn while had significant difference in other seasons. In summer these components were in lower amount. The reason could be attributed to various environment parameters. Such as gradual fluccuations in water/air temperature. Another reason may be assocated with rate of glycerol consumption in these two examined amphypods. In winter, the storaged glycerol was used for prevention from freezing while in the autumn. The glycerol was consumed follow by lowest amounts n cold/winter season. This study showed that the amphypods are suitable organisms for monitoring of aquatic environments by measuring of biochemical components, especially glycerol.

In modern life, access to fresh vegetables is limited and proper processing and storage techniques for vegetables are essential. Cabbage quality decreased over time, especially after 2-month storage. The bleaching and freezing can affect it. It is important for the industry to know how to treated cabbage after harvest till supermarket to decrease after harvest damage. The results of this research showed that vitamin C, phenolic compound and antioxidant decreased with storage. Vitamin C is decreased more than other characteristics of cabbage after storage almost 90%. Blanching methods affect the nutrient content of cabbage and Both freezing and storage times decreased cabbage texture (Turkmen et al. 2004). Therefore, in this study, a factorial experiment based on RCBD was designed with 3 replicates to investigate the effect of blanching and freezing method over time on frozen cabbage. by measuring the amount of vitamin C, phenolic compounds, antioxidant activity and elements, the effects of blanching (boiled water and steam) and freezing of two methods domestic (-18 °C) and semi-industrial (40° C) on the nutritional value of celery (Appium graviolens var. Dulce) was evaluated. The results showed that in general, after six months, the antioxidant activity,
vitamin C and phenolic compounds decreased and blanching with boiling water significantly reduced the amount of vitamin C and antioxidant capacity compared to blanching with steam treatment in celery. Over first two months, the reduction in phenolic compounds and antioxidant capacity was more than that of four and six months, but declined over time as the process slowed down. As time progressed, all elements reduced in celery. Vitamin C, has many biological activities in the human body. More than 85% of vitamin C in human diets is supplied by fruits and vegetables (Sahlin et al. 2004). The content of vitamin C among Brassica vegetables varies significantly between and within their subspecies. Generally, among Brassica vegetables, white cabbage is the poorest source of vitamin C (Saari et al. 1995). However, it is a very popular species of Brassica vegetables in Iran. According to our results, freezing of cabbage more than 2 months decreased vitamin C drastically. Loss of vitamin C after 7 and 14 days’ storage at 20 °C decreased to 44% and to 28% in broccoli However, when broccoli was stored at 4 °C, and did not decrease after 7 days and 20% loss after 21 days. Vitamin C content in broccoli reduced by 26% after 3 days of storage at room temperature, by reducing of the storage temperature to 5 °C resulted in an increase (25%) of vitamin C content in broccoli (Lisiewska and Kmiecik 1996). Before vegetables freezing, they washed and blanched to inactivate enzyme systems, especially oxidative enzymes (e.g. polyphenoloxidase, ascorbic oxidase, peroxidase) (Perry and Klein 1982). The effect of blanching in the retention time of vitamin C in different Brassica was investigated, although it depends on temperature and time of blanching method. Vitamin C after blanching was 84% for cauliflower, 70% of cabbage, loss of vitamin C was 28–32% in cauliflower, 41–42% in broccoli, 34% in broccoli (Leja et al. 2001). There was different report was shown that freezing decreased vitamin C in different Brassica differently. After blanching and freezing, about 30% in broccoli. The freezing did not change vitamin C in broccoli and
cauliflower during a 12-month storage and decreased by 3–18% for broccoli and 6–13% for cauliflower and 30%for cabbage (Saari et al. 1995; Leja et al. 2001). there are some reports was shown that Phenolic compounds in broccoli, which are stored 7 days at 1 °C, showed more decreased than ascorbic acid. Storage time effect on Phenolic compounds in different Brassica (Lisiewska and Kmiecik 1996). Phenolic compounds in broccoli, which are stored 7 days decreased 2–3 times (Papetti et al. 2002) On the contrary, total polyphenols did not significantly change in broccoli during 7 days’ storage (Leja et al. 2001; Lisiewska and Kmiecik 1996). Generally, among the qualitative characteristics of celery, reduction of vitamin C more than other traits was affected by different treatments. the type of freezing did not have a significant effect, but storage time was significant so that the values were reduced more rapidly in the first two months of storage.

Manipulation of protein-polysaccharide interactions provides an opportunity to control the stability and texture of food emulsions. Proteins are surface-active ingredients and can function as effective emulsifying agents also polysaccharides with polyelectrolytes capacity can impart excellent colloidal stability to emulsion droplet by a combination of electrostatic and steric mechanisms. The aim of this study is production of reduced-fat mayonnaise using electrostatic and covalent of &beta-lactoglobulin and Farsi gum as stabilizer, as well as elimination of cholesterol and reducing application of other stabilizers.

At first, electrostatic and covalent complexes of &beta-lactoglobulin and Farsi gum with dry and wet heat treatment were produced. Then Farsi gum and all of the complexes with 1% wt concentration were used in the mayonnaise. The samples were compared in terms of droplet size distributions, apparent viscosity, firmness, color and microscopic analysis.

Comparison of mean droplet size of the samples showed that mayonnaise contained dry-heated (for one week at 60 ºC and relative humidity of 75%, 1:1 biopolymer mixing ratio) &beta-lactoglobulin-Farsi gum complex (D1/11) had the smallest size. Mayonnaise containing Farsi gum and W/1:2/6.69 (wet-heated &beta-lactoglobulin-Farsi gum complex with 1:2 biopolymer mixing ratio and pH=6.69) had mean droplet size similar to commercial mayonnaise. Mayonnaise containing Farsi gum showed non-uniform microstructure by microscopy observation. Commercial mayonnaise had the highest apparent viscosity followed by mayonnaise containing Farsi gum. Firmness result also had the same trend (P<0.05). Commercial samples showed significant differences and the highest yellowness compared to others due to the presence of egg.

According to the above results, &beta-lactoglobulin-Farsi gum complexes can be used as emulsifier and stabilizer in mayonnaise and salad dressing.

Regarding the nutritional value and availability of acorns in west and southwest of Iran, the effects of partial substitution of wheat flour with acorn flour was investigated. Wheat flour was replaced by treated acorn flour in the formulation of sponge cake and the quality characteristics were evaluated. Density of cakes was increased with increasing the acorn flour. As the substitution degree was increased the textural parameters such as hardness, adhesiveness, gumminess and chewiness were increased and some other characteristics including springiness and cohesiveness were decreased. Sensory attributes also diminished as the concentration of acorn flour increased. Heat treatment of acorns did not exhibited significant effect on the cakes quality. Based on the results obtained in this research work, in order to make the cake as a functional food, lower levels of substitution (less than 10%) had acceptable texture and sensory properties as compared to the control

Producing food colors from natural sources, such as tomatoes, which could be used instead of synthetic colors, is important in relation to health and safety aspects of the consumers. In this research, solvent extracting was carried out by using three non-polar solvents, including petrolium ether (bp=55°C), n-hexane (bp=60°C) and a mixture of n-hexane: ethanol: aceton (1:1:2, bp=50°C). Also, two different temperatures (room temperature and boiling point of the solvent) and three different periods of time (2,4 and 6 hours) of extraction were tried. Using mixture of the solvents (at boiling point) for six hours showed to be the optimum conditions for extraction and there were significant differences between this treatment and other conditions for extractions. In addition to the above results, the same volumes of the solvent and samples were found to be the best ratio in comparison with the other volumes ratios. It was significant at P .05.. The yield of color extraction from tomatoes and tomato powder was 0.14 and 0.24% (w/w), respectively. Also, the purity of the extracted color was 82.65% lycopen. The extracted color showed a good stability in sunflower oil for three months at 4°C.

This research was carried out to determine the optimum conditions for acidic extraction of gelatine from cattle bones. The effects of different treatments such as pH (1.8 to 2.6), temperature (60 to 80°C) and time (40 to 120minutes) on yield of extraction, gel strength, viscosity and absorption (420 nm) was measured. Coefficients of central composite rotatable design (CCRD) and regression surface response.... (RSREG) were used. The coefficients for yield, gel strength, viscosity and absorption were 0.990, 0.991, 0.998, 0.928, respectively. According to variance analysis of the data, there were significant differences between the effects of three variables (pH, temperature and time) on yield of extraction and viscosity, but no significant difference was observed between the effects of temperature and pH on gel strength and absorption, respectively. Comparisons of the averages of functional characteristics of acidic and commercial gelatin showed that viscosity and strength of acidic gelatin were higher than those of commercial one. The optimum conditions were found to be: pH=1.88, temp. 80°C and 118 min., to reach the highest yield and pH=1.88, temp. 60°C and 48 min. to get maximum strength, and pH=2.56, temp. 69°C and 118 min. to obtain the maximum viscosity and finally, pH=2.6, temp. 62°C and 48 min. for minimum absorption. Also, the results revealed that there were significant differences between trace elements content (Fe, Ca and Pb) of commercial and acidic gelatins. Although, acidic gelatin contained higher amount of the elements, but these amounts were still less than maximum values which are allowed to exist in gelatin. Amino acid compositions of both gelatins were almost identical.

Vegetable crops are exposed to repeated spraying against the pests and diseases during the growing sessions. Crops such as greenhouse cucumber are usually harvested a short time after spraying, bearing a high level of pesticides with them. This fact justifies a research on this problem. This research was carried out to determine the effects of metalaxyl (a fungicide commonly used in greenhouse) residue in cucumbers grown under traditional and modern greenhouse conditions. Cucumber plants were sprayed with two different doses: 1/1 000 and 2/1000 metalaxyl in modern and traditional greenhouses. Samples were collected 1, 2, 4, 7, 10, 14, 21 and 28 days after spraying with metalaxyl. The fungicide residue was extracted, cleaned up by TLC and the final solution was analyzed by HPLC. Data were analyzed statistically by t-test (paired observations) and Duncans multiple range test. The results revealed that the amount of metalaxyl residues increased in the first four days following spraying. This amount of the residue was 10 times higher than MRL (0.5 mg/kg), while a lower level of MRL (0.5 mg/kg) was detected 21 days after the application. Results also showed that a lower dose of application left a higher residual level than the higher one. On the other hand, no significant differences (p<0.05) were found between the amounts of residues in peeled and unpeeled cucumbers. Thus, fruit peeling does not reduce the fungicide residue in cucumber. Also, there were no significant differences (p<0.05) between the results obtained from modern and traditional greenhouses. Therefore, cucumber must either be harvested before or at least 14 days after spraying with metalaxyl.

Three potato cultivars (Solanum tuberosum L.), spring Marfona, autumn Cosima and Marfona, obtained from Isfahan Province, were stored under different conditions: 4oC, darkness 12oC, fluorescent light room temperature, darkness and room temperature, daylight. Relative humidity of the above stores was 85 to 90%. Analyses were carried out to determine total glycoalkaloids, α-solanine, specific gravity and total solids, during 30 days for the spring cultivar and 90 days for the autumn cultivars in stored tubers. α -Solanine content of each cultivar was determined by HPLC, in mg per 100 g dry weight, at the beginning, the midinterval, and the end of storage periods. Also, amounts of α-solanine in autumn cultivars, which showed the highest levels after storage for sixty days, were determined before and after heat processing for frying and cooking. Data were analyzed statistically by completely randomized design with Duncan’s multiple range test. The results revealed that the amount of α-solanine of the spring cultivar considerably increased under 12oC and flourescent light as compared with the other storage conditions. The amounts of α-solanine considerably increased during the storage of autumn cultivars which were stored at 12oC under flourescent light compared with those stored at room temperature and daylight, room temperature and darkness, and at 4oC and darkness, respectively. The amount of α-solanine decreased due to spring conditions during the storage period for cultivars stored at room temperature and darkness. In spite of the decreasing effect of peeling, before frying and after cooking, frying and cooking processes had no effect on the amount of α-solanine in the samples. Therefore, according to the above results, the most appropriate storage conditions to prevent increasing effect of α-solanine in potato tubers was 4oC in darkness.

Citrus juice and concentrate are among the major industrial products in Iran. Large quantities of citrus peels with considerable amounts of pectin are also produced. Pectin could be extracted from the peels to be used as a gelling agent in some food products. Unfortunately, all pectin used in the food industry is imported. This study was carried out to find the best conditions of pectin extraction from orange peels. Samples of orange peels were collected from a concentrated orange juice factory. Pectin was extracted by sulfuric, nitric and hydrochloric acids. Certain qualitative and quantitative tests, such as yield of extraction and cost of extraction were determined. Also, food safety aspects as a result of using the above acids were considered. Hydrochloric acid was found to be the most suitable acid. In the second part of the research, the effects of temperature (85 and 90˚C), time (40, 50 and 60 minutes) and pH (1.6, 1.8 and 2.0) on the yield of extraction, purity, gelling ability, percentage of methoxylation and degree of esterification of the extracted pectins were measured. Statistical analysis of the results showed that the best conditions for the extraction of pectin include a pH level of 1.6, a temperature of 90˚C and a period of 50 minutes.

Iran, as the biggest producer of dates, has 25 percent of the world date production. More than 400 varieties of dates have been identified in Iran. Dates have a high nutritional value they contain considerable amounts of sugar, vitamins and minerals. For instance, 2830 kilocalories of energy are obtained from one kilogram of dates. Dates are economically suitable replacements for sugar in bee feeds. However, feeding bees with some varieties, which contain certain amounts of mannose and potassium, are harmful. This investigation was carried out in order to identify the most suitable variety of dates useful for bee feed and to determine its nutritional value as human food. In this study, the dominant varieties of date including Bam Mazafati, Behbahan Kabkab, Booshehr Kabkab, Jiroft Mazafati, Kangoroad, Khanizi, Khasi, Saameran, Shahani, Shahdadi and Zahedi, were collected and analysed. The amounts of moisture, fat, protein, ash, and minerals such as sodium, potassium, and calcium were determined. Also, sugar composition of the above varieties consisting of glucose, fructose and sucrose, was measured. Chemical methods (Lane-Eynon and Shaffer-Somogyi) and high performance liquid chromatography were used for sugar determination. The results showed that, except for moisture and protein contents, there was no significant difference between fat and ash contents of the samples. Shahdadi and Zahedi had the highest and lowest moisture contents, respectively. Behbahan Kabkab and Shahdadi had the highest and lowest protein contents respectively. Jiroft Mazafati and Saameran had significantly the lowest amounts of potassium and mannose, respectively, while Kangoroad had significantly the highest amount of sucrose, which is use full for bees. Therefore, these three varieties were found to be suitable replacements for sugar in bee feeds, but considering their price, Saameran is recommended as the best feed for bees. On the other hand, with respect to the minerals and sugar contents, Behbahan Kabkab was found as the most nutritious date for human food.

Recent restrictions on the use of artificial food colours have prompted extensive research on natural pigments as suitable substitutes. The red beet Beta vulgaris</em> L. has been found as a suitable source of natural red pigment since beet powder and concentrates can conveniently be used in food products. In this research, hot and cold extractions of randomly selected red beets were used after preparation and blanching. The extracts were filtered and passed through a column of Amberlite XAD-7 resins after adjusting the pH to 2.5. The adsorbed colour compounds were extracted from the resins by eluting the column with acidified alcohol. The solvent was removed by vacuum rotary evaporator and solid colour was obtained, using a freeze dryer. Physicochemical properties of the solid colour were determined by measuring ash and sugar contents of the solid colour. Also, its purity and stability were investigated under different conditions. The amount of ash and sugar were negligible. The highest stability was observed at a pH value of 5 under cold and dark storage conditions. The purity of the solid colour was determined by scanning in the range of visible wavelengths and preparative TLC. The result showed that purity of the colour as a hundred percent betacyanins. The yield of production was calculated as 2.7 g powder per kilogram of beet. The colour was used in producing ice cream and frozen sherbets, which showed to be a suitable replacement for artificial colour carmoisine. Also the production cost of the colour was as low as less than 0.1 percent of that of ice cream or frozen sherbets.