فهرست مطالب mahdi ayyari

Kelussia odoratissima (KO) and Angelica sinensis (AS) have been used in their indigenous traditional medicine, for various diseases. This study was conducted to evaluate the volatile oil composition of KO leaves (KVL) and AS root (AVR) and biological activity of essential oils (EOs) and hydroalcoholic extracts of both plants using two different transgenic zebrafish (Danio rerio) models.

Both EOs were isolated by hydrodistillation and analysed by GC and GC/MS. For viability tests, larvae were treated with different concentrations of extracts to determine an appropriate starting concentration. Hydroalcoholic extracts and EOs have been tested in a dose-dependent manner for their biological activity using tissue-specific transgenic zebrafish Tg(fli-1: EGFP) and Tg (ins: GFP-NTR) embryos and larvae. One-way ANOVA was used to compare the mean of pBC area and intersegmental vessels (ISVs) outgrowth between the treatment groups.

Eleven compounds were in common to both oils, comprising 51.3% of KVL and 61.7% of AVR, of which 39.3% in KVL and 37.6% in AVR were phthalide structures. Results revealed that both EOs blocked ISVs formation in the Tg (fli-1: EGFP) embryos increased to 10% of the control value, while both hydroalcoholic extracts did not show any anti-angiogenesis effects in these embryos. In addition, AVR has been shown to significantly induce PBC regeneration following ablation in the Tg (ins: GFP-NTR), but its regenerative activity was lower than that of 5′-N-ethylcarboxamidoadenosine (NECA) as a positive control. Taken together, the anti-angiogenesis activity of both EOs could be attributed to the phthalide structures while for the PBC regenerative activity, other compounds including β-Thujaplicinol, exclusively existing in AVR, might be effective.

Although the genera, organs, and origin of these plants are different, their similar chemical composition and biological activities make them valuable resources for further investigation in basic medical and pharmaceutical science.

In this research, the effects of foliar application of humic acid were evaluated on morphological traits of chicory (Cichoirium intybus L.) and pergularia (Pergularia tomentosa L.) in aeroponic system. Aeroponic system is a suitable method for investigating the effects of nutrient elements and optimizing growth of different plants in controlled conditions. The treatments were including control and 200, 400 and 600 mg/L humic acid. Foliar application of fertilizers was done at 20, 40 and 60 days after seed culture in aeroponic system. After 4 months, the morphological traits were measured. The results of ANOVA indicated that the effects of treatment were significant for plant height, root length, number and area of leaves, fresh and dry weights of the shoot and root in chicory and pergularia. The results in pergularia showed that 600 mg/L resulted in the highest plant height, root length, volume root, leaf number, dry and fresh weights of root and dry and fresh weights of shoot and the lowest of the mentioned traits were observed in the control. In the chicory, the results showed that the highest and lowest values of plant height, root length, volume root, leaf number, dry and fresh weights of root and dry and fresh weights of shoot belonged to the highest concentration of humic acid and the control treatment, respectively. It can be said that the use of a 600 mg/L of humic acid in the aeroponic system can improve the quantitative characteristics of the pergularia and chicory.

In this study Eryngium noeanum Boiss. from four different regions of Alamot (AL) (Qazvin province), Ahuan Col (AH) (Semnan), Razaqan (RZ) (Markazi province), and Bashm Col (BA) (Semnan) and also, Eryngium iranicum Mozaff. from Nikpey village (NP) (Zanjan) at elevations of 1600, 1980, 2450, 2150, and 1400 m above the sea level were collected during the summer 2018. The shade-dried plant materials were hydro-distillated by a clevenger apparatus. Qualitative and quantitative analyses of the essential oils were carried out by gas chromatography-mass spectrometry (GC-MS) and GC-FID, respectively. The essential oil contents were obtained as 0.3, 0.4, 0.55, 0.2, and 0.1% (w/w) for AL, AH, RZ, BA, and NP, respectively. The main components of essential oils were amorpha-4,11-diene (14.0, 17.6, 9.0, and 13.6%), δ-selinene (21.5, 40.5, 23.1, and 35.7%), spathulenol (4.6, 0.7, 6.4 and 5.5%), eudesm-7(11)-en-4-ol (3.4, 0.5, 6.7, and 4.4%), and cis-falcarinol (20.3, 1.0, 20.0, and 15.2%) for AL, AH, RZ, and BA, respectively. Also, the main components of the essential oils in NP sample were n-octanal (12.8%), myrtenol (12.5%), and cis-falcarinol (13.5%). The main compounds in different populations of E. noeanum and E. iranicum essential oils were sesquiterpenes (about 70-90%) and non-terpene (56%), respectively. Eryngium noeanum Boiss. is a medicinal rangeland and native plant of Iran, has not been given due attention. Despite prickly appearance, it has a big aerial part with considerable essential oil contents including valuable sesquiterpenes and polyacetylenic compounds ranking it among the highly commercial essential oils worldwide. This calls for many research studies in different fields as extensive biological studies on the essential oil components of this plant can greatly help determine and standardize its quality.

The smoke produced from natural substances such as medicinal plants is used in various cultures for different purposes. The use of medicinal fumes has been reported in nearly 50 countries. Among medicinal plants, Tussilago farfara L. known as coltsfoot has been introduced in Canon the famous book of Avicenna a Persian polymath, for chronic dry cough and various pulmonary diseases and shortness of breath. T. farfara is distributed in wet mountainous regions of Iran. For this study, the leaves and flowers of T. farfara were collected from Chalous Road in Iran. The smoke from the burning of T. farfara organs was prepared by homemade glassware trapping the smoke in methanol and then methanol was evaporated. In general, five grams of materials were burned and the smoke was dissolved and trapped in 100 ml of methanol. The trapped and dried materials from the smoke of extracts were filtered and injected into the GC/MS for analysis and identification of its constituents. 51 compounds representing 91.1 and 92.3 percent of smoke extracts of T. farfara were identified in leaf and flower. Also, 57 compounds were detected in the sample of EL and EF with 96.8 % and 97.7 %. The percentage of phenolic compounds that were identified in all extracts of smoke were SL and SF with 52.1 and 46.5, respectively. Phenol, Hydroquinone, P-Cresol and O-Cresol were the major compounds in the smoke extracts. Smoke leaves and flowers of T. farfara were selected to test the antimicrobial to continue. This study examined the bactericidal effect of smoke flowers. Fractions of effective constituents with the help of hexane-ethyl acetate with the method of thin layer chromatography (TLC) were isolated. The results of this experiment showed that a fraction (8:2) of hexane-ethyl acetate inhibited the bacteria Staphylococcus aureus. But Escherichia coli was not inhibited.

Iris species are known to be a rich source of isoflavonoids. Iris germanica L. is one of the most important species of bearded irises which is known as Irsa in traditional medicine. In this study, the amount of phenolic compounds and antioxidant activity of the waste obtained during the essential oil extraction process of 34 samples of German iris rhizomes (6 parent samples, 27 hybrid samples and 1 commercial sample) were evaluated. Iris samples were collected from the Ornamental Plants Research Center (OPRC) in Mahallat city and commercial sample were purchased from the market. After extracting the EO, samplings were carried out using the extract in balloon. The phenol content was measured by Folin-Ciocalteu method, flavonoid by Aluminum-chloride method and antioxidant activity with DPPH and FRAP assay. The results of variance analysis showed that all measured factors were affected by the type of hybrid, which was significant in all factors (P<0.01). The results of mean comparison showed that samples with purple and violet flower colors had the highest amount of phenol and flavonoid, followed by the highest level of antioxidant activity. In other words, the highest amount of flavonoid (113.22 mg QUE/g DW plant) and antioxidant activity (DPPH) (817.73 mg TBHQ/gDW plant) was observed in sample P6 (purple parent) and the highest amount of phenol content (261.31 mg GAE/g DW plant) was observed in sample P3 (violet parent). In general, the water residues from the Iris EO extraction can be used as a suitable and cheap source for obtaining natural antioxidants.

Green basil (Ocimum basilicum L.) is a medicinal plant widely used in the food, cosmetics, and perfumery industries. Cucumber mosaic virus is one of the most common plant pathogens worldwide. In the present study, the effect of cucumber mosaic virus (strain Fny) infection on the phytochemical and physiological properties of three green basil cultivars under growth chamber conditions (21 ± 2 ° C and 16-hour light/8 hour dark period) was investigated. The plants were mechanically-inoculated in the true two-leaf stage with the virus. Mean comparison of the studied traits showed that the virus significantly reduced photosynthesis, the content of chlorophyll a and b, total chlorophyll, and total carbohydrates in the infected plants. In contrast, the content of total protein, amino acid proline, total phenol, flavonoids, and the percentage of essential oils (w/v) in infected plants was increased. The results of essential oil analysis of three basil cultivars by Gas chromatography and Gas chromatography-mass showed a significant difference in essential oil compounds between control and viral treatments of each cultivar. The compounds Camphor, Carvone, Estragole, Methyl Eugenol, Beta-caryophyllene Alpha bisabolene, and Caryophyllene oxide make up more than 70% of the essential oil content. Results of the present study indicated that viral infection causes physiological and phytochemical changes in virus-infected plants. Increasing and/or decreasing the phytochemical content and some essential oil compounds depends on the interaction of the virus and the basil cultivar and may be in line with the susceptibility or resistance of the cultivars to the virus infection.

Baneh (Pistacia atlantica Desf.) is one of the main wild species of Pistacia in Iran. The exudate of this plant is an oleo-gum-resin that comes out of the outer layer of plant bark and is called “Saghez” in Iran. In this study, the content and chemical composition of oleo-gum-resin essential oil from thirteen habitats in Iran, including: Lamerd, Gachsaran, Dehpabid, Jiroft, Marivan (Govaz, Ghamishleh, Anjiran, Haneh sheikhan), Kahnoj, Shorigeh, Dalaho, Andimeshk, Ilam were investigated. The essential oil extraction was carried out through hydro-distillation by the Clevenger apparatus for three hours. Identification and quantification of individual components were performed by GC/MS and GC-FID, respectively. The results showed that the highest and the lowest essential oil content (26.6% and 9.5%) belonged to Marivan (Govaz) and Jiroft, respectively. Also, the essential oil content of Shorigeh (25%), Marivan-Anjiran (25%), Marivan-Ghamishleh (24.6%) was desirable. In total, thirty-nine compounds were identified. α–pinene, β–pinene, camphene, sabinene, δ-2-carene, α–phellandrene, α-terpinene, ρ–cymene, cis-limonene oxide, ρ–cymenene, trans-pinocarveol, cis-isocitral, neo-iso-verbanol and myrtenal were identified as main constituents. Also, monoterpene hydrocarbons were the main group of essential oils in all samples and they formed 81.1% to 97.9% of components.

Nepeta crispa Willd. Locally known as Mofarrah belongs to Lamiaceae family. It is an Iranian endemic self-growing medicinal plant to Alvand Mountains in Hamadan province. N. crispa has carminative, anti-inflammatory, antinociceptive, antibacterial and antifungal properties. The aim of this study was to investigate the phytochemical composition of essential oil compounds of this plant in the vegetative stage, which cultivated in Tarbiat Modares University of Tehran at an altitude of 1283 m above sea level, and compare with samples taken from natural habitats in Arzanfood and Gashani with the altitude of 3015 and 2489 m above sea level, respectively. The plants were harvested during the late July 2018. The essential oils of all samples were obtained by hydrodistillation and were analyzed by GC-MS and GC-FID. The yield of essential oil in the sample collected from Arzanfood, Gashani and field samples were 2.13, 1.97 and 0.55% (w/w), respectively. Twenty components were identified among the samples, representing 93-96% of the oils. The main components were 1, 8-cineole (40.25%, 48.5, 49.9%), 4aα, 7β, 7aα-nepetalactone) 34.8%, 3.7%, 24.7%) and β- pinene) 6.2%, 5.4%, 5.4%) for cultivated field, Gashani and Arzanfood samples, respectively. Although the percentage of oil and 1, 8-cineole in the cultivated sample were low, but the amount of nepetalactome compounds was high. According to the results, the environmental conditions had an important role in the qualitative and quantitative changes in the essential oil of this plant but cultivation of N. crispa in a place with more than 1200 m difference in altitude, lower than the main habitat, was a promising sign for domestication of N. crispa. Optimization of other factors including, cultivation in an area close to the natural habitat, nutrition, biotic and abiotic stresses treatment could even improve the quality of this plant compared to the self-growing ones.

Iran is the largest producer of saffron and more than 90 percent of Iran's saffron is produced in Iran.Drying is one of the methods commonly used to increase the shelf life of medicinal plants and agricultural products. In this study, drying process of saffron petals with thin layer hot air dryer at five levels at 40, 60, 80, 100 and 120 ° C and three air flow of 0.5, 1 and 1.5 m/s and their impacts of on colored parameter (amounts R, G and B) have been studied. By using vision machine system, color images of the saffron dried petals were taken, and analyzed using image processing algorithms, Features colored (RGB) were also obtained. In addition, the anthocyanin content, (using differential pH method) and L*a*b* characteristics were measured in order to determine the proper drying conditions of the samples. The data were analyzed using factorial experiment based on randomized complete design. The results showed that maximum RGB values were at the temperature range of 80 to 100°C, representing the greatest ratio and intensity of red, green and blue colors. Also in this temperature range, the highest anthocyanin content (482.21mg/l) and lowest color changes (ΔE) were obtained which was a relative desirability of this range for drying saffron petals.

In order to study the effect of N rate and plant density on grain yield and N use efficiency of blessed thistle, a field study ‎was carried out in a randomized complete block design with factorial arrangement of treatments in three replications‏ ‏at ‎Research Field of Tarbiat Modares University in 2013 and 2014 growing seasons.‎‏ ‏Treatments comprised of four pure N ‎rates (0, 50, 100 and 150 kg N ha-1)‎‏ ‏from urea source and five plant densities (5, 10, 15, 20 and 25 plants m-2).‎‏ ‏The main ‎effects of nitrogen and plant density were significant for grain yield, NUE, plant height, SPAD value, root to shoot weight ‎ratio and N content of grain. Increasing N rates increased grain yield, SPAD value, plant height and grain N, but agronomic ‎efficiency, N recovery and utilization were decreased by increasing of N rate; Higher densities resulted in decreased grain ‎yield and chlorophyll index, while improved NUE throughout increment of root to shoot weight ratio and plant height‏.‏‎ ‎Novel findings of this study could be used for N management. ‎

In this study, a sensitive, accurate and reproducible high performance liquid chromatographic method was developed for determination of diosgenin from fenugreek (Trigonella foenum-graecum) leaves in 22 ecotypes of Iranian fenugreek. The obtained results showed that the highest concentration of diosgenin with 23.8 mg g-1dw was found in Boshruyeh ecotype. While, Mashhad ecotype with 3.51 mg g-1dw diosgenin contained the lowest concentration of diosgenin. The method employed in current study confirmed significant variations in the diosgenin concentration in Iranian fenugreek. Therefore, Iranian fenugreek can be considered as a new potential crop and source of diosgenin in the pharmaceutical industry.

Quinces use as bearing fruit tree and also have numerous medicinal effects, such as high contents of antioxidants including phenols and flavonoids. Due to importance of flavonoids in the physiological and medicinal aspects of this tree, the current research was conducted to investigate the variations of flavonoids in the leaves and fruits of quince genotypes from northern regions of Iran, comparing them with cultivar Esfahan as control. So, it was followed by using 24 genotypes including ARD1-7, Beh Amroudi, Beh Sibi, Beh Givi Kowsar from Ardebil province, LA1 and LA3 from Tehran provinces, M1, M2, M3, M4, M8 and M9 from Khorasan and AS1, ASM1, ASM2, ASM3, ASP1 and ASP2 from Guilan province. The results showed significant differences in the total flavonoids in the leaves and fruits of the quinces. According to the results, M3 and LA1 genotypes respectively had the highest and lowest total levels of the flavonoids in their leaves with 21.9 and 3.1 mg QE/g values, compared with the control cultivar containing 13.4 mg QE/g. Also the results of the flavonoid contents of the fruits indicated the highest level in control cultivar, Esfahan with 6.2 mg QE/g, while all other genotypes had significantly lower levels of flavonoids. Also evaluation the effects of sampling period on the variation of total contents of the leaf flavonoids showed that the highest concentration of these compounds belonged to the beginning of the summer, while the leaves had lower levels of flavonoids at other sampling periods.

The present study is the first assessment of the fatty acids of leaf and essential oil compositions of new three habitats of aerial parts of K. odoratissima. The aerial parts of K. odoratissima from the three habitats were dried. The essential oils were obtained by hydrodistillation for 3 h in a Clevenger-type apparatus, then the analysis of the components was carried out using gas chromatography–mass spectrometry. To study the oil yield and fatty acids, the dried leaves subjected to extraction in hexane by using Soxhlet Apparatus. To analyze fatty acids from the oil fractions by gas chromatography technique, the oil was subjected to transesterification to obtain the fatty acid methyl esters, which, were dissolved in hexane and subjected to GC analysis. According to the results, a total of 43 components, the major constitutes of essential oil compositions were (Z)-Ligustilide (76.45%), Unknown-A (4.47%), (E)-Ligustilide (2.57%), (Z)-Butylidene phthalide (2.37%), 5-pentyl cyclohexa-1,3-diene (1.57%) and Kessane (0.77%) in K. odoratissima. The sixteen fatty acids were separated from the oil (5% yield per 100 g dry matter). Linoleic acid (25.46%), α-Linolenic acid (16.66%), Palmitic acid (11.92%), Oleic acid (9.33%), Stearic acid (4.72%), Petroselinic acid (2.53%), Arachidonic acid (2.51%) and Erucic acid (1.76%) were major fatty acids. Generally, K. odoratissima is a rich source of essential fatty acids and phthalide derivatives, specially (Z)-ligustilide. This study was presented valuable information about the phytochemical properties, which can be useful for the future researches on the pharmacological effects of K. odoratissima.

Recently, due to healthier and nutritional problems of synthetic antioxidants in food processing, using of herbal medicine and its effective components as a natural resources with antioxidant properties have beenevaluated. Different organs of Hawthorn have more important in food and medicinal science due to high antioxidant properties and various flavonoids and proanthocyanin content. This study was accomplished in order to examine the total phenolic content and antioxidant capacity of hawthorn different organs (fruit, leaves and flower) in 56 accessions. Antioxidant capacity and total phenolic content were determined using ferric-reducing antioxidant power (FRAP) and Folin–Ciocalteu assays. The amount of total phenolics was significantly (P<0.05) different in the both amongst species and different plant organs ranging from 7.21 to 87.73 mg GAE/g dry weight. Total phenolic content was in its highest value in the flowers of G7 (87.73 mg GAE/g DW), whereas the lowest level was found in the flowers of G4 (7.21 mg GAE/g DW). The Antioxidant capacity was widely different in the both species and different organs of the individuals ranging from 0.22 to 1.84 mmol Fe ++/g DW. Antioxidant capacity was in its highest values in the fruits of G1 (1.84 mmol Fe 2+/g DW), whereas the lowest capacity was found in the leaves of G18 (0.22 mmol Fe 2+/g DW). However, differentorgans and species of the genus Crataegus showed a high level of total phenolic content as well as antioxidant capacity.These results showed that different species of Crataegusespecially C. pseudomelanocarpa and C. pentagynaare promising sources of natural antioxidants and other bioactive compounds beneficial without any harmful effects to be used in the food or the pharmaceutical industries.