جستجوی مقالات مرتبط با کلیدواژه "پارامترهای بیوشیمیایی" در نشریات گروه "علوم پایه"

Distribution of macroscopic algae is high on the shores of the Persian Gulf and the Sea of Oman. On the other hand, sustainable and successful aquaculture depends on maintaining the health of the aquatic species and improving the breeding conditions to achieve the maximum growth in aquatic species and also reduce the costs of the production process. Researchers are looking for new and better approaches to achieve the goal of aquaculture. Using seaweed in aquaculture is expanding due to the presence of nutrients such as antioxidants, essential fatty acids (omega 3 and 6), essential amino acids, vitamins, minerals, carbohydrates and beta-carotene. Using algae in the diet of aquatic animals not only reduces the cost of feeding, but also leads to improve the efficiency of aquatic nutrition, digestion, and strengthening the immune system of fish, and by improving the efficiency of digestion, it also affects the quality of water. Given the importance of grey mullet, Mugil cephalus as one of the commercially important fish species and its ability to grow in earthen ponds as well as adapting to a wide range of temperature, salinity and nutritional conditions, in this research, the effect of brown algae, Stoechospermum marginatum extract on growth performance and some biochemical parameters in the grey mullet blood serum were studied for 60 days.
The experiment was conducted in a completely randomized design with 120  grey mullet (with average weight of 9.56 ± 1.02 g) in 4 treatments and 3 replicates (n = 10 in each replicate) including: control group (T1) without using algae extract, 5 (T2), 10 (T3) and 15 g (T4)  brown algae per kg of diet. In order to measure the growth performance, at the end of the experiment, weight and length of fish were recorded. Chemical analysis of the carcass composition was carried out according to the AOAC standard method. Also, in order to determine the biochemical parameters, blood was taken from the heart using a syringe, and poured into a 2 mL- micro tube. Then, it was centrifuged at 3000 rpm for 10 min and serum was separated and stored at -70°C. Analysis of the data obtained from the measurement of growth indices, carcass composition and some biochemical parameters of blood serum in grey mullet were performed using One-way analysis of variance (ANOVA) and Duncan's multiple range comparison tests.
The results showed that at the end of experiment, the highest final weigh (32.89 ± 2.74 g), the highest weight gain (292.56 ± 35.01%) the lowest food conversion ratio (0.95 ± 0.05), the highest voluntary feed intake (1.74 ± 0.42%), the highest crude protein (20.50 ± 1.29%), lowest crude lipid (6.73 ± 1.13%), the highest globulin (4.27 ± 0.12 g/dL), albumin (2.63 ± 0.18 g/dL) and total protein (6.84 ± 0.54 g/dL) were observed in T4. In all of these parameters, T4 showed a significant difference compared to control treatment (p<0.05). The lowest cholesterol and triglyceride were observed in T3 and T4 exhibiting a significant difference compared to the control (T1; p<0.05).
The present results suggest that diet containing 15 g/kg Stoechospermum marginatum extract could enhance growth, carcass quality and serum biochemical parameters in Mugil cephalus.

Saffron for 3000 years ago was valued as culinary condiment, dye, perfume and medicinal herb. The aim of research was to examine the effects of saffron supplementation on glucose levels, lipids and liver enzymes. In present study, the homogenate of saffron and saffron treated with chitosan was extracted after drying using 96% ethanol. Then, ethanol solvent was removed using rotary equipment. 60 Wistar rats (200-250g) were divided into six groups, including normal group, saffron group, saffron group treated with Chitosan, isoniazid group, isoniazid-saffron group and isoniazid-saffron group treated with Chitosan. For induction of oxidative effect, oral extract of saffron and saffron treated with chitosan was used as gavage. The DPPH method was used to compare the antioxidant effects of chitosan extract. The use of extract of saffron led to decreasing of triglyceride(31%), cholesterol(35%), LDL(22%), ALP(16%) and increasing of HDL levels until to 32%. Chitosan-treated saffron extract also led to decreasinf of LDL to 21%, ALT to 7% and increasing of HDL to 26%. In group receiving isoniazid and saffron, serum levels of enzymes of ALT(7%-16%), ALP(26%-69%), cholesterol(3%-31%), triglycerides(18%-44%) and glucose(4%-14%) showed a significant increasing compared to the control group. Therefore, considering these positive effects of saffron and chitosan-treated saffron, it is obvious the enhancing effects of the evaluated factors. Regarding the effects of saffron on blood glucose and triglycerides and also Liver enzymes, they have a positive effect on blood glucose and lipid control. The results of this study confirmed the undesirable effects of isoniazide on the cells of body.

Currently, zinc and its compounds are used as a suitable material for the treatment of infection in burns, open wounds and chronic wounds. This investigation, was designed and carried out with the aim the local effect of green zinc nanoparticles on blood biochemical parameters and skin tissue repair during wound healing in laboratory rat.

In this experimental study, 42 male Wistar rats were divided into 7 groups of 6. Then, by creating a 2 cm burn on the back of the rats, treatment was immediately performed with zinc nanoparticles ointment (1%, 4%, 16%) and zinc oxide ointment and pure Vaseline for 21 days. After sampling from the percentage of wound repair in the skin of rats was calculated. Similarly, blood sampling was done from the hearts of rats and serum was used to check biochemical parameters.

The percentage of wound healing in the 4% ointment group and zinc oxide ointment was significantly higher than the control group. In addition, all the concentrations of ointment were safe in terms of their effect on liver enzymes.

Conclution: 

The results of investigation showed that the ointment of 4% zinc nanoparticles was the best treatment group, which accelerated the repair of skin wounds. Based on the results of the present experimental study, probably the ointment produced by the identified green nanoparticles could be suggested as a strong repair agent in skin lesions naturally.

This present resent study investigated sperm motility parameters (sperm motility duration and sperm motility percentage), spermatocrit, density of sperm and some biological factors such as sperm seminal plasma indices (ionic and organic composition) in 8 Acipenser ruthenus and Acipenser gueldenstaedti broodstocks. Sperm mobility duration length, percentage of sperm mobility, spermatocrit, density of sperm were measured 185±10.23 s, 90.00±2.25 %, 2.60±0.19 %, 2.42 × 0.30, respectively in A. ruthenus and 210±11.12 s, 90.33±2.16 %, 2.77±0.24 %, 2.63±0.18, respectively in A. gueldenstaedti. The concentrations of Sodium (Na+), Potassium (K+), Calcium (Ca2+) and Magnesium (Mg2+) ions were determined 88.56±7.24, 4.24±0.32, 7.52±0.29 and 2.38±0.26 Mm L-1, respectively in A. ruthenus and 90.84±6.12, 4.65±0.21, 7.31±0.34 and 2.44±0.31 Mm L-1, respectively in A. gueldenstaedti. The concentrations of serum cholesterol and glucose were calculated 48.74±5.46 and 27.90±2.57 mg ml-1, respectively in A. ruthenus and 47.53±4.22, 28.63±2.31 mg ml-1 respectively in A. gueldenstaedti. In addition, seminal plasma of A. ruthenus and A. gueldenstaedti were estimated 1.53±0.30 and 1.58±0.34 mg/dl protein, respectively. The results of this study showed that there is a significant difference between sperm motility duration in A. ruthenus and A. gueldenstaedti. No significant differences were observed between other biochemical and spermatological parameters in A. ruthenus and A. gueldenstaedti.

Purchase, transportation and maintenance of fish became and important chain in aquaculture industry along with the increase of farmers. Transporting of fishes is an stressful process in aquaculture. The present study investigate the effect of adding NaCl in transporting stress, on haematological parameters, blood serum biochemical parameters and skin mucus of Caspian white fish. For this purpose, along with the transporting fish to laboratory different concentrations of NaCl (0, 1, 3 and 6) were added to the trasnporting water. The samples of mucus and blood were taken immediately after transferring. Fish transferred to the seawater for one week and samples were collected after three days and one week. The result of this study showed that the RBC and hematocrit increased significantly over time after the salinity stress (P<0.05) and the amount of the hemoglobin, blood glucose and the total number of skin mucus bacteria decreased significantly over time after the salinity stress (P<0.05). However, there was no significant change in the amount of white blood cell, differential count of white blood cells, hemoglobin, mean volume of cell, the average concentration of cell hemoglobin and cortisol (P>0.05). Generally, it can be concluded that transported fishes with 1 g/litr salt in transporting water had better condition regarding immune parameters compared control group and other treatments.

This present resent study investigated sperm motility parameters (sperm motility duration and sperm motility percentage), spermatocrit, density of sperm and some biological factors such as sperm seminal plasma indices (ionic and organic composition) in 7 beluga (Huso huso) and Starry sturgeon (Acipenser stellatus) broodstocks. Sperm mobility duration length, percentage of sperm mobility, spermatocrit, density of sperm was measured 310±30.65 s, 93.11±4.31 %, 2.78±0.31 %, 2.76 × 109±0.36, respectively in beluga and 81±23.61 s, 80.63±3.27 %, 2.63±0.23 %, 2.40×109±0.28, respectively in A. stellatus. The concentrations of Sodium (Na+), Potassium (K+), Calcium (Ca2+) and Magnesium (Mg2+) ions were determined 96.24±8.47, 3.34±0.40, 8.66±0.44 and 1.15±0.15 Mm L-1, respectively in beluga and 95.33±8.40, 4.23±0.28, 8.43±0.47 and 2.39±0.24 Mm L-1, respectively in. The concentrations of serum cholesterol and glucose were calculated 12.98±6.77 and 32.67±2.47 mg ml-1, respectively in beluga and 45.68±5.78, 30.43±0.29 mg ml-1 respectively in A. stellatus. In addition, seminal plasma of beluga and starry sturgeon were estimated 1.45±0.27 and 1.49±0.29 mg/dl protein, respectively. The results of study indicated that mobility during length, percentage of sperm mobility and magnesium values had significant difference between beluga and A.stellatus sturgeon. The quality and quantity of sperm in Huso huso are higher than that in Acipenser stellatus. In addition, the magnesium in Acipenser stellatus level was significantly increased compared to Huso huso, but no significant differences were observed between both species.

The present study investigated sperm motility parameters (sperm motility duration, sperm motility percentage), spermatocrit and concentration and some biological aspects of sperm by determination of seminal plasma indices (ionic and organic composition in Beluga, Huso huso,</em>). Survey was done on 8 wild fish and 8 cultured fish during March and April in 2017 in Propagation and Culture of Sturgeons center, Shahid Marjani, in Gorgan Sperm motility duration, sperm motility percentage, spermatocrit and sperm concentration in wild Beluga were measured 310±61.65, 81.11±6.31, 2.33±0.31, 2.50±0.36 and in cultured Beluga were determined 170.43±34.61, 65.63±5.81, 1.26±0.23, 1.41±0.28 respectively. The concentrations of Sodium (Na+), Potassium (K+), Calcium (Ca2+) and Magnesium (Mg2+) ions were in wild Beluga were measured 99.36±9.47, 4.96±0.40, 7.75±0.44 and 1.15±0.15 MmL-1 and incultured Beluga were determined 98.29±10.40, 5.03±0.28, 7.76±0.47 and 2.12±0.24 MmL-1</sup> respectively. The concentrations of cholesterol and glucose in wild Beluga were measured 49.33±8.77, 32.65±2.12 and in cultured Beluga were determined 47.47±4.78, 32.21±1.66 respectively. Also Seminal plasma in wild Beluga and cultured Beluga was contained 1.10±0.27, 1.33±0.29 mg/dl protein respectively. Studies showed that between duration of motility, motility and concentration Magnesium (Mg2+) ions in wild Beluga and cultured there are significant difference.

In order to improve the quality and efficiency of caviar crop from sturgeon as assessment of ovarian maturation stage is inevitable;also it should be avoided from capture of fish at stage of follicular atresia.In this study determination of atretic follicle in ovary was done by evaluation of blood steroid level and biochemical parameters. After catheterization, 10 fish that follicles in reabsorption stage were seen in their ovaries were selected for further analysis. Blood testosterone and estradiol values were significantly lower in A. ruthenusthat containing atretic follicle in their ovaries in compare to control. Blood biochemical parameters including, triglyceride, cholesterol and calcium were also lower in fish with atretic follicles in their ovary in compare to control. Our findings showed that biochemical and hormonal changes caused by follicular atresia could be used successfully to distinguish atretic follicles from normal follicles in A. ruthenusto replace biopsy as an invasive method.

In the current study, the effects of different levels of probiotics, Lactobacillus plantarum in the diet of Asian sea bass, Lates calcarifer on body composition, serum biochemical parameters (glucose, cholesterol, triglyceride and albumin) and liver enzymes (lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase) were investigated. This experiment was carried out with three treatments and a control group for a period of four weeks. The treatments were included 0, 1×106, 2×106, 3×106 colony forming unit (CFU) gram probiotic in each gram of basic diet. Fish with an average weight of 50.26 ±0.89 grams were randomly distributed in tanks and in each tank stocked 20 pieces of fish. Feeding was done twice a day. The obtained results indicated that the use of different levels of probiotic in diet of Asian sea bass had no significant effects on serum biochemical parameters and liver enzymes. The different levels of probiotic did not affect protein, ash and moisture values between the treatments and the control group (P> 0.05). Moreover, fat value in the fish T2 and T3 was significantly higher (P< 0.05) than the fish control and T1. Also, nitrogen free extract (NFE) value varied significantly (P < 0/05) between the control and the fish T3. Overall, this study indicated that this probiotic had no significant effect on some blood biochemical parameters and liver enzyme, but at the high level can positively influence on body composition of Asian sea bass.

In this study، the effects of hormonal Ovaprim، HCG and pituitary extract injection on biochemical parameters of seminal plasma (Na+، K+، Ca+، Mg2+، cholesterol and glucose) in snow trout (Schizothorax zarudnyi) males were compared. Twenty males at Zahak hatchery with a body weight of 784±77 g were randomly assigned to four treatment groups. The spermiation of males was stimulated with ovaprim، HCG، cPG and saline physiological (control) at the following doses: 0. 3 ml/kg، 400IU/kg، 0. 2 mg/kg and 0. 3 ml/kg، respectively. The results showed that there were a significant difference of seminal plasma Na+، K+، glucose and cholesterol among treatments (P<0. 05). As the highest value of Na+، K+، glucose and cholesterol observed in ovaprim treatment (91. 10±3. 14 mmol/lit، 78. 00±3. 43mmol/lit، 0. 043±0. 03 mg/dl and 0. 073±0. 03 mg/dl respectively). Likewise there was a highly significant difference of seminal plasma Ca+ among treatments (P<0. 05)، as the highest value of Ca+ observed in ovaprim and pituitary extract treatments (7. 46±0. 99 and 7. 17±0. 51 mmol/lit). The results showed that there were no significant difference in Mg2+ among treatments (P>0. 05). The present study demonstrated that ovaprim، HCG and pituitary extract have different effects on biochemical parameters of seminal plasma in snow trout، and ovaprim has more effects on biochemical parameters of sperm than HCG and pituitary extract.

Some biological aspects of semen were investigated in the Beluga، Huso huso، by determination of seminal plasma indices (ionic and organic composition)، osmolality and their relationships with spermatozoa motility. The concentrations of Sodium (Na+)، Potassium (K+)، Calcium (Ca2+) and Magnesium (Mg2+) ions were 84. 88±18. 67، 3. 33±0. 31، 0. 92±0. 2 and 0. 78±0. 23 MmL-1 respectively. Seminal plasma contained 0. 065±0. 028 mg/dl protein، 26. 40±7. 85 mg/dl cholesterol and 20. 61±7. 04 mg/dl glucose. Semen spermatocriet was 2. 39±0. 42%، pH 7. 75±0. 49 and duration of spermatozoa movement 319. 27±72. 48 second. The Sodium/Potassium and Calcium/Potassium ratios were25. 48 and 0. 27. The osmolality of seminal plasma ranged from 36 to 96 mOsmol Kg-1. The significant positive correlations were observed between some seminal plasma indices، Na+- spermatozoa motility (r=0. 764، p<0. 05)، osmolality – Na+ (r=0. 742، p<0. 05) and protein- glucose (r=0. 821، p<0. 01). There were also significant negative correlations between Ca2+-K+ (r=-0/703، p<0. 05)، and pH- osmolality (r=-0. 779، p<0. 05). Consequently، variability in the ionic composition and osmolality of seminal plasma of fishes، mainly due to intra-specific differences in testicular secretions. Our data can be used to improve methods for short and long-term storage of Beloga semen.

In this study، the effects of hormonal GnRHa، HCG and pituitary extract injection on biochemical parameters of seminal plasma (Na+، K+، Ca+، Mg2+، total protein، cholesterol and glucose) in goldfish (Carassius auratus) males were compared. Fish injected with hormonal GnRHa، HCG and pituitary extract with 10μg kg−1، 1500 IU/Kg−1، 3mg/Kg−1 respectively. There were a highly significant difference of seminal plasma Na+ among treatments (p<0/05)، as the highest value of Na+ observed in GnRHa and HCG treatment (202. 33±15. 04، 195. 66±10. 69 mmol/l respectively). There was a highly significant difference in K+ among treatments (p<0/01) as the highest value of K+ observed in control group (31. 40±1. 24 mmol/l)، but There were no significant difference in Ca+ and Mg2+ among treatments (p>0/05). There was a highly significant difference of seminal plasma total protein and glucose among treatments (p<0/05)، as the highest value of total protein and glucose observed in HCG treatment (0. 06±0. 001 g/dl، 0. 13±0. 04 mg/dl respectively). Also There were a highly significant difference of seminal plasma cholesterol among treatments (p<0/01)، as the highest value of cholesterol observed in HCG and pituitary extract treatment (0. 018±0. 001، 0. 020±0. 003 mg/dl respectively). The present study demonstrated that GnRHa، HCG and pituitary extract have different effects on biochemical parameters of seminal plasma in goldfish، and HCG have more effect on biochemical parameters of sperm than GnRHa and pituitary extract.