جستجوی مقالات مرتبط با کلیدواژه « عصاره اتانولی » در نشریات گروه « زیست شناسی »

Two-spotted spider mite, Tetranychus urticae (Acari:Tetranychidae), is one of the most important pests in plants, especially crops that it has high reproduction and ability to develop resistance to chemical pesticides. Considering the increasing of environmental pollution due to chemical pesticides, the use of plant extracts has received much attention as an alternative method due to their acaricidal activity. Therefore, this experiment was performed to determine the chemical compounds of Momordica charantia fruit extract and toxicity of hexane and ethanolic extracts of this plant on adults of two-spotted spider mite in a completely randomized design with the control in four replications for fumigant and contact toxicities and repellent activity. After 24 hours, the mortality of mites was recorded. The results of bioassays showed that the mortality rate of mites and the repellent effect elevated with increasing the concentration of M. charantia extracts. The hexane extract of M. charantia at the concentration of 1000 μg/cm2 had the highest mortality (75.56%) in contact toxicity. Ethanolic extract at the concentration of 1000 μl/l air had the highest mortality (79.89%) of mite in fumigant toxicity. The repellent effect increased to 80%, 5 hours after application of ethanolic extract by increasing the concentration from 200 μl. In M. charantia fruit extract, sixteen different compounds were identified in which monoterpenoids, linalool oxide, limonene oxide, and 8-hydroxilinalool, had the highest amount. Therefore, the ethanolic extract of M. charantia in high concentrations can be recommended due to its monoterpenoids to protect plants, especially crops, against two-spotted spider mite.

Treatment of cancer by medicinal plants has long been of interest to researchers. In the meantime, some plant species contain substances that, through apoptosis or necrosis, can inhibit or eliminate cancer cells. The family of mint and chicory are among the medicinal plants that have many biological effects.The purpose of this study was to investigate the effect of Pennyroyal and Artemisia extract on gastric cancer cell line (AGS).In this study, aerial parts of plants were extracted. AGS cells were treated with the concentrations of Hydroatanolic extract (50-1000 g / ml) at 24, 48 and 72 hours intervals. Cell motility was estimated using MTT method and the rate of induction of apoptosis by flow cytometry with The Annexin-V .The results of MTT assay showed strong and dependent inhibitors of the concentration of cancer cell proliferation by various extracts of Achillea and Salvia. These extracts have a doseand time dependent anti cancer effect on AGS cells. In the study of apoptosis in treated cells, the extract of the Pennyroyal was more effective. Due to the cytotoxic effects of hydrotanolic extracts on AGS cells, these plants can be used as potential option for further studies on cancer treatment. Therefore, the purification of the active ingredient in these extracts and the determination of their effect mechanism is recommended.

Aim and Anaplastic thyroid cancer, including the most dangerous type of thyroid cancer.and as the most modern cancer treatments are ineffective with side effects, it seems essential to find impressive alternatives treatment. Lavender plant with the scientific name Lavandulla anguostifouliyaof familyLamiaceae, is a Perennial plant that has high nutritional and medicinal value. Therefore, the cytotoxic effect of ethanolic extract of Lavender plant on 8305C cell lines was evaluated.

In this study, for the first time, the cytotoxic effect of ethanolic extract of Aerial organsLavender plant on 8305c cell lines was evaluated. The Lavender plant in April 2016 of sehezare Tonekabon were collected andstudied; Then the antioxidant activity was evaluated by DPPH0 Assay. 8305C cell line was cultured in DMEM with 10% fetal bovine serum. Different concentrations of ethanolic extract of Lavender plant (5, 10, 20, 30, 40 and 50 mg/ml) for 24, 48 and 72 hours were applied on cancer cells 8305C. Then, the viability of cells using MTT assay was considered.

In this study, the free radical scavenging DPPH0 equals to 6/27mg/ml was calculated. The results of MTT assay showed that the ethanolic extract of Lavender plant has a cytotoxic effect on the 8305C cell line which is dependent to their doses. Also IC50 of the extract concentration for 8305C cells was obtained to be 5 mg/ml in time 48 hours.

The ethanolic extract of Lavender plant which is dose-dependent could destroy the cancer cells and showed cytotoxic properties. Thus, this plant can be used as a medicinal plant against anaplastic thyroid cancer in wider scope.

Aim and Among cancers, Lung cancer is considered to be the most important cause of death in worldwide and as the most modern cancer treatments are ineffective with side effects, it seems essential to find impressive alternatives treatment. Nettle plant with the scientific name Urtica dioica L. Of family Urticaceae, is a flowering and Perennial plant that has high nutritional and medicinal value. There fore, the cytotoxic effect of ethanolic extract of nettle leaf (Urtica dioica L.) on A549 cell lines was evaluated.
In this study, for the first time, the cytotoxic effect of ethanolic extract of nettle leaves on A549 cell lines was evaluated. The dioecious nettle leaves in April 2015 of sehezare Tonekabon were collected and studied; Then the antioxidant activity was evaluated by DPPH0 Assay. A549 cell line in DMEM with 20% fetal bovine serum was grown. Different concentrations of ethanolic extract of two legs nettle leaves (5, 2/5, 1/25 and 0/5 mg/ml) for 24, 48 and 72 hours were applied on cancer cells A549. Next the viability of cells using MTT assay was considered.
In this study, the percent of free radical scavenging DPPH0 equals to 67/79 percent and the amount of IC50 was calculated to be 4/30 mg/ml. The results of MTT assay showed that the ethanolic extract of nettle leaves has a cytotoxic effect on the A549 cell line which is dependent to their doses. Also IC50 of the extract concentration for A549 cells was obtained to be 5 mg/ml. MTT results showed no significant differenc in 48 and 72 hours. But the difference was significant at 24 hours.
The ethanolic extract of nettle leaves which is dose-dependent could destroy the cancer cells and showed cytotoxic properties. Thus, this plant can be used as a medicinal plant against lung cancer in wider scope.

Nowadays, cancer is knownas the main cause of death throughout the word. In recent decades, medical and molecular advances have not only been able to find the reasons and mechanisms of cancer, but they were more successful in early diagnosis and treatment of it. Citrus skin is the accumulation center of many antioxidant and enzymes. Flavonoids and limonene are among the important compounds in the citrus skin and their vital rolesin prevention and treatment of cancer is under study these days. In this research, after cultivation and reproduction of MCF-7cancer cells line and in order to determine, the effectof cellular cytotoxicity of citrus skin extract, these cells were put in exposure to different doses of etanolic extract and after 72 hours, MTT test was done. Results indicate that the ethanolic extract of Orange skin at concentrations 7.5,10 (p ≤ 0.05)and the ethanolic extract of Limon skin at concentrations 2.5,5 (p ≤ 0.01)decrease cell growth significantly in comparition with the control group. the effectof cellular cytotoxicity grows with an increase in the concentrationof extract and the highest percentage of growth inhibition in extract of Orange skin and Limon skin is related to the concentrations of 7.5 and 5 respectively. Thus citrus skin extract is suggested as an effective material in curing cancer in medical advances.

Today there is great interest in using traditional medicine for ulcer healing has been increased.Quercus persica is used in Iranian traditional medicine to treat some gastric diseases. The present study was undertaken to evaluate the Quercus persica ethanolic extract for gastric ulcer healing in rats. Extractedfrom theskin of oakfruit soakedin90%ethanoland extractswere measuredby therotarydevice. To determine the effect of this extract from Quercus persica, 56 wistar male rats divided into 2 groups; experimental and control (n=7).Gastric ulcers were induced by luminal application of 0.12 ml acetic acid solution (40% v/v). One day after ulcer induction, the experimental groups Ethanolic extract of Quercus persica at concentration 100 mg/kg w.b.and the control groups received 1 ml normal salin through oral gavage for few consecutive days. On days 1, 3, 5 and 7 rats were killed and wound samples were collected for histological and statistical studies. Results analysis by one way Anova demonstrated that ethanolextract ofthe fruitskinof Quercus persica at concentration 100 mg/kg w.b.significantly increased gastric ulcer area compared to control group and decreased ulcer healing. Also, inflammation cells such as neutrophil and macrophage in the third and fifth days in the experimental groups compared with control groups had significantly increased, which is prolonged inflammation stage.On the seventh day, in the experimental groups a significant reduction in the amount of fibroblast was seen that indicates the delay in wound healing and collagen-synthesis process.The current study showedsignificant increase of inflammation and decrease wound healinginrats treated with ethanolextract ofthe fruitskinof Quercus persica at concentration 100 mg/kg w.b.as compared to control group

This study aimed to investigate the effects of Myrtus communis extract in 10, 7.5, 5, 2.5, 1.25, 0.625, 0.312, 0.156 mg/ml concentration on Hela and MCF7 cell lines after 72 h using the MTT assay. Hela and MCF7 cells were cultured in RPMI1640 medium containing fetal bovine serum, penicillin and streptomycin and incubated at 37 ° C atmosphere containing 5% CO2 and 95% humidity in sterile flasks. Results show that ethanolic extract of M. communis in 1.25 mg/ml (p ≤ 0.05), and 0.312, 0.156 (p ≤ 0.02) concentration, was significantly reduced Hela cell growth compared to control, after 72 h. The highest percentage of growth inhibition at 0.625 mg/ml concentration was % 82.33. For MCF7 cells, ethanolic extract of M. communis in 2.5, 1.25, 0/312 mg/ml (p≤ 0/02) and 0.625 (p≤ 0/001) concentration was significantly reduced MCF7 cell growth compared to control, after 72 h. The highest percentage of growth inhibition at 1.25 mg/ml concentration was 70.64 %. The results suggest that ethanolic extract of M. communis has inhibitory effects on cell growth both on Hela and MCF7 cell line.

Breast cancer is the second most pervasive cancer among women. Breast cancer is a worldwide epidemiological problem which the current remedies cannot heal it effectively and has unwanted side effects. Therefore, attempt to produce more effective medicine with less toxicity is essential. Ferula gummosa is anti-cancer, anti-spasm, anti-paroxysm, antiseptic and laxative and it is tonic for stomach, reconstructs surface scars, and causes more milk producing in breasts. Since, its anti-tumorous effects have not been studied on the breast cells; the cellular toxicity effect of its ethanolic extract was investigated in this study. To investigate the preventing effects of F. gummosa ethanolic extract on cellular growth, we have used the MCF7 (Human Caucasian breast adenocarcinoma) cell line which has been cultivated in RPMI environment containing cow's embryo serum and antibiotic. Then we treated these cells in different concentrations of F. gummosa ethanolic extract (0.165, 0.312, 0.625, 1.25, 2.5, 5, 7.5, and 10 mg/ml) for 72 hours. The amount of cell viability was determined by MTT method. Ethanol extract of F. gummosa has a significant cellular toxicity effect on the MCF7 cell line in 0.625, 1.25, 2.5, 5, 7.5, and 10 mg/ml densities after 72 hours compare with control group, and the calculated IC50 for F. gummosa extract was 1.765 mg/ml. The results suggest that ethanol extract of F. gummosa has preventing effect on the cellular growth of MCF7 cellular class.