جستجوی مقالات مرتبط با کلیدواژه « broccoli » در نشریات گروه « زیست شناسی »

Diabetes is one of the main causes of death in developing countries, which can be improved by various factors such as physical activity and proper diet. The purpose of this study was to investigate the effect of broccoli powder consumption along with combined training on some selected hepatokines in men with type 2 diabetes. This research was semi-experimental and practical type and was pre-test and post-test. For this purpose, 44 diabetic male volunteers were randomly divided into 4 groups of 11 people including control (placebo recipient), supplement (supplement recipient), exercise + placebo groups, and exercise + supplement. The training protocol included 45 minutes of resistance training with an intensity of 60-70% of one maximum repetition, 30 minutes of aerobic exercises (running) with an intensity of 60-70% of the maximum heart rate. Broccoli (supplement) was also prepared in powder form and 10 grams per day was given to each volunteer for 12 weeks. Blood samples were taken 48 hours before the first training session and 48 hours after the last training session and were used to analyze the indicators. Intergroup analysis of data was done using a two-way analysis of variance and a correlated t-test was used for intra-group differences. A significant difference between the groups was observed in ANGPTL3 and ANGPTL4 (P<0.001). Also, the examination of changes within the group showed that in supplementary, exercise, and supplementary exercise groups, a significant decrease was observed in ANGPTL3 and ANGPTL4 (p < 0.05). The results of the present study showed that 12 weeks of combined exercise along with broccoli supplementation improves selected hepatokines and insulin resistance. This improvement was greater in the group that exercised and took supplements at the same time.

This research was conducted in order to investigate the application of melatonin and potassium sulfate on the quantitative and qualitative characteristics of broccoli (Brassica oleracea var. italica) under water stress. A factorial experiment was performed in a completely randomized design with three replications. The experimental factors included irrigation conditions at two levels (50 and 100% FC), melatonin at three levels (0, 150 and 300 µM) and potassium sulfate at three levels (0, 2 and 5 mM). The results showed that in the treatment of 300 µM melatonin and control irrigation (100% FC), the leaf area was twofold greater than in the treatment of 50% FC without the use of melatonin. The highest activity of the catalase enzyme was observed in the interaction of 50% irrigation level and 150 µM melatonin and the highest amount of malondialdehyde was observed in the same field capacity without the use of melatonin. The highest amounts of chlorophyll a and b were obtained in the interaction of 100% FC, 150 μM melatonin and 5 mM potassium sulfate. The highest amount of head yield was achieved in the interaction of 100% FC with the use of 300 µM melatonin and 5 mM potassium sulfate, and in this treatment, the highest nitrate reductase enzyme activity and the lowest nitrate accumulation in the crop head were observed. The highest content of proline and activity of the peroxidase enzyme were observed under conditions of 50% FC without the use of melatonin and potassium sulfate. The best treatment in most of the interaction traits was melatonin 300 µM, potassium sulfate 5 mM under control irrigation conditions (100% FC). Therefore, the use of these compounds is recommended to improve the physiological characteristics, yield and quality of broccoli.

Broccoli has been well known as a plant of high nutritional value. Application of broccoli peroxidase in different areas of clinical biochemistry, biotechnology and food industry enhances the interest for further study on the enzyme. The objective of this study was to evaluate the kinetic parameters of broccoli's peroxidase and their variations in the presence of methanolic extract of some endemic plants from Mazandaran. In this investigation, the crude extract from broccoli was homogenized, centrifuged, precipitated by ammonium sulfate and dialyzed. The enzyme activity was monitored in 414 nm with H2O2 and ABTS in phosphate-citrate buffer. The specific activity of the peroxidase was determined 0.58 U/mg in crude extract and 3.61 U/mg in dialysate. The optimum pH and temperature of the enzyme were obtained 4 and 30°C respectively. The extract of Artemisia tschernieviana, Eryngium caucasicum, Rosmarinus officinalis, Sambucus ebulus, Ligularia persica, Crataegus douglasii, Papaver rhoeas, Viscum album and Allium ursinum were prepared by maceration method. The enzyme activity was measured in presence of the extracts in different concentrations. The kinetic results shown that the extract of Crataegus douglasii, Rosmarinus officinalis, Papaver rhoeas and Artemisia tschernieviana in 33 µg/ml concentration inhibited the enzyme as 88.2, 79.5, 78.6 and 76.3% respectively. The extract of Ligularia persica shown no effect on maximum velocity of reaction while reduced the Km value by 4 times. Allium ursinu showed a partial inhibition on the peroxidase. Therefore, study on these plants for achieving to effectors on broccoli’s peroxidase, with pharmaceutical, agricultural and industrial applications could be recommended.

With the increasing use of herbs in natural treatments, this branch of complementary medicine has found a special place in the treatment of diseases. Herbal medicine in diseases and especially infectious diseases has improved in recent years. The antimicrobial and antifungal and anti-cancer effects of these plants in Iran have been the source of many researches and at the end, this research can lead to self-sufficiency in the country's pharmaceutical industry. Among these plants, could be considered to broccoli with medicinal and many antimicrobial and antifungal properties. Broccoli with scientific name. Brassica oleracea L is a member of the Brassicaceae cabbage family. The important thing about the impact of this plant is its climatic condition, which will be effective on its anatomical structure which is an important goal of this research. In this study, specimens of two Different Climates from Arak and Theran was perepared then The broccoli vegetative and reproductive organs were cut and fixied in 90% Ethanol alcohol fixatin. Then After the preparatory steps, including cleaning and washing,stained and were studied by photomicroscope. Interestingly, with regard to the tissues resistant to this plant, climate change has had no effect on broccoli. Only the amount of leaf parenchyma and number of vessels in The petiole, leaf, stem and anther organs were effective. Finally, it can be concluded that due to the resistance of this plant, it can be used in different climates without worrying about changing its properties to the use of this plant for medicinal purposes.