جستجوی مقالات مرتبط با کلیدواژه "cell viability" در نشریات گروه "زیست شناسی"

In recent years, significant efforts have been focused on advancements of novel biomaterials based on natural polymers and utilization of efficient methods such as skin tissue engineering for wound treatment. In this study, a 3D printed polycaprolactone (PCL) scaffold coated via immersion in a 1:4 blend of 40% silk fibroin from Bombyx mori cocoons and TEMPO-oxidized was developed. The pore size and the porosity were 180 µm and 85%, respectively. The results demonstrated an enhancement in exudate absorption (swelling and water uptake of 1342% and 80%, respectively), improvement in storage modulus (G’) from 500 to 4000 Pa, as well as viscoelasticity up to 60%, which all are favorable for wound dressing applications. Moreover, the wettability and biodegradability studies revealed an overall increase in contact angle and degradation rate of 19.9°±3, and 95%, respectively. Cell viability and migration studies on fibroblastic cells (L929) using MTT assay, DAPI/ Phalloidin staining, and scratch test showed over 90% viability up to 7 days and complete scratch repair within 24 hours. These findings show that 3D printed PCL scaffolds coated with silk fibroin and oxidized nanocellulose are promising for wound healing applications and might pave the way to natural polymer-based wound dressings.

The avian stem cells are an excellent in vitro model for development and pharmacology educations. These cells have the potential for self-renewal and differentiation and can be considered as a valuable technology in the poultry industry. The purpose of this study was the derivation, culture, and comparison of chicken fibroblast cells to quail cells, regarding cell doubling time and the rate of cell viability during various cell passages. In this experimental study, fertilized eggs were obtained and fibroblasts were isolated with an enzymatic digestion method and of cultured in DMEM medium including 10% FBS for various passages. Population doubling time (PDT) and cell viability were evaluated by trypan blue staining and MTT assay. Data indicated that both cells had a maximum proliferation when cultured in a DMEM containing 10% FBS at 38 C˚. Based on our results, PDT was measured 16±2 h for both two cells during the first o third passages. But, the population of the chicken fibroblasts was doubled in number each 28±2 h, while this value was 36±2.1 h for quail cell population (p<0.05) during the third to sixth passages. Also, cell viability results were according to PDT results. This study demonstrated a potential of self-renewal and cell viability of quail fibroblast cells as a new avian cell source and suggested to use of these cells in future manufacturing applications.

Breast cancer is the most common cause of death from cancer among women. The triple-negative breast cancer (TNBC) is the most invasive subtype, and chemotherapy is the only therapy option. Cancer cells preferably utilize the glycolysis pathway even with proper oxygen availability, and this activation plays a great role in tumorigenesis. Therefore, glycolysis targeting can be an effective strategy for cancer treatment. Here, the apoptotic effect of a glycolysis inhibitor named dichloroacetate (DCA) on TNBC cells MDA-MB-231 was assessed, and the expression of anti-apoptotic genes and oncogenic miRNAs was evaluated. MTT assay showed that DCA reduces cell viability in a dose-dependent manner with the IC50 concentration of 50 mM. Annexin/PI assay demonstrated that DCA due to DCA treatment. Finally, the expression of anti-apoptotic genes Bcl2l1 and Mcl1 and oncogenic miRNAs miR21 and miR27a decreased due to DCA treatment. Our results confirmed that DCA, as a glycolysis inhibitor, leads to apoptosis induction in TNBC cells because of reducing expression of viability genes and miRNAs.

It is a continuing effort to develop new anticancer compounds. Some studies have reported that some compounds of Achillea Santolina have inhibitory effects on several cancer cell lines. The present study aims to evaluate the cytotoxic effect of the hydroalcoholic extract of Achillea Santolina on MCF-7 human breast cancer cells in fibrin hydrogel. In this experimental study, to prepare the fibrin hydrogel scaffold, an M199 medium containing 10% FBS, 1% penicillin/streptomycin, fibrinogen powder with a concentration of 3 mg/ml, and thrombin with a concentration of 120 u/ml was used. Cultured MCF-7 cells in fibrin gel were treated with hydroalcoholic extract of Achillea Santolina in 25, 50, 100, 250, 500, and 1000 mg/ml concentrations for 24h. Morphology and cellular viability and proliferation were evaluated by DAPI staining and MTT assay at certain days after treatment. Also, the structure of the scaffold and the condition of the cells in the gel were investigated by photographing with a scanning electron microscope.The results of this study showed the cytotoxic effects of Achillea Santolina extract in a dose- and time-dependent manner. The concentration of 500 mg/ml of the extract of A. Santolina was determined as an IC50 concentration. Also, cell viability in the control group and treated group with extract at IC50 concentrations showed significant (P<0.05) differences on days 1, 3, and 5. The hydroalcoholic extract of Achillea Santolina can inhibit MCF-7 cell proliferation and induce cell death in a dose and time-dependent manner.

The worldwide incidence rate for cancer has been rising. However, the molecular mechanisms involved in tumor growth and metastasis are unclear. Autophagy is a cellular pathway that leads to cell death or survival depending on the specific condition of the tumor cells. The aim of this study was to evaluate the effects of activation or inhibition of the autophagy pathway using tunicamycin or N-acetylcysteine on cell viability in the MDA-MB-231 breast cancer cell line.

MDA-MB-231 cells were cultured in the presence of different doses of tunicamycin or N-acetylcysteine. Then, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was performed for the evaluation of tumor cell viability. Real-time PCR was carried out to evaluate the expression of the genes encoding for Becline-1 and the mammalian target of rapamycin (mTOR) (as autophagy markers) in the MDA-MB-231 cell line. The LC3-II to LC3-I ratio and p62 were measured with western blotting.

Tunicamycin significantly increased autophagy markers and inhibited cell viability in a time- and dose-dependent manner. A negative significant correlation was observed between the expression of autophagy markers and cell viability. However, N-acetylcysteine resulted in decreased autophagy and increased cell viability just at a concentration of 2mM.

Tunicamycin leads to activation of the autophagy pathway, resulting in a decrease in cell viability in breast cancer cells (MDA-MB-231); and N-acetylcysteine, at a low concentration, can inhibit autophagy and increase cell viability. Thus, the autophagy pathway can be considered a target in cancer treatment.

 

Actinomycetes are a good resource to discover new drugs based on their abundant secondary metabolites. Because both fungal and human cells are eukaryotes, the metabolites of fungal antagonist actinomycetes may also inhibit the growth of cancer cells.

The antagonistic activity of 24 actinomycete strains that inhibited the growth of cells of a plant pathogen, Phytophthora capsici, was investigated against 4 other plant pathogens. The siderophores production of two selected strains that showed the highest antagonistic activity against pathogens was evaluated. The cytotoxicity effects of two strains on the SW480 cell line was measured using MTT assay in vitro and the IC50 percentage was determined.

Only two selected strains had 30 and 54% inhibitory effect against Phytophthora drechsleri, respectively. The strain 408 had no inhibitory effect against Pythium ultimum, while the strain 6010 completely prevented Pythium ultimum growth. The strain 6010, unlike the strain 408, controlled the growth of Rhizoctonia solani and Fusarium oxysporum pathogens about 70%. Only the strain 408 was able to produce siderophore. The significant effect of the treatment on colorectal cancer cells was observed at 2.47 and 2.71% (v/v) concentrations for both 408 and 6010 strains, respectively.

The results of this study confirmed our hypothesis that secondary metabolites of antagonistic actinomycetes can lead to cancer cells death. The use of fungal or oomycete cells is introduced as an easy, safe, and inexpensive method for screening actinomycetes that are candidates for the discovery of new anticancer drugs.

Lung carcinoma is the second most common type of cancer. Inefficiency of the current treatments and the undesirable side effects of chemotherapy drugs made the know-how of the treatment important. The purpose of this study is to investigate the synergic effect of curcumin and Cisplatin in comparison with the sole application of each treatment on Calu-6 cell line, an epithelial cell line of human lung carcinoma, and the expression of Cdc42 gene. The viability of Calu-6 was examined after 24- or 48-hour treatment with doses of 0.5 to 8 µg/ml of curcumin, 0.1 to 50 µg/ml of cisplatin and combined doses of curcumin and Cisplatin by MTT assay. To measure apoptosis and the expression of Cdc42 gene, flow cytometry and Real-Time PCR were utilized. Decrease of cell viability and induction of cell death were observed in the cells treated with 0.67 µg/ml of curcumin and 1.7 µg/ml of cisplatin (the lowest effective dose) and the combined treatment with the same doses of each drug after 24-hour treatments. The maximum rates of early and late apoptosis were related to treatment with curcumin and the combined treatment. The gene expression analysis results indicated that both Curcumin and Cisplatin decrease the expression of Cdc42 gene, moreover, their co-administration showed synergic effects. Therefore, Curcumin could be an appropriate option for complementary administration with other chemotherapy agents in order to reduce their efficient dose, and to reduce their side effects.

Despite many efforts, no definitive treatment is yet knowing for cancer. Therefore, research on new compounds that have anti-cancer properties is especially important. The aim of this study is investigation the cytotoxic effect of Girard-T reagent-based Schiff bases and their complexes on HT29 cancer cells. HT29 cells was cultured in DMEM/F12 medium containing FBS 10% and antibiotics 1% then the effects of ligands and their complexes with the concentration of 0.1, 1 and 5 mg/ml were surveyed on these cells in 1, 2, and 3 days. Growth, proliferation and morphological changes were photographed using an inverted microscope. MTT assay and DAPI staining were used to quantify cell viability. With concentrations of 0.1, 1, and 5 mg/ml of [SnMe2(L2)] (the effective component) the survival were decreased to 34, 24 and 18% after 72h(P

The microbiota of breast-fed infants are mainly consists of Bifidobacterium and Lactobacillus. Normal vaginal delivery has an important role in the colonization of beneficial bacteria and proper development of the immune system and gastrointestinal tract in comparison with infants born through cesarean section. In this study we examined the effect of microbiota of a 4-month-old breastfeeding infant born with normal vaginal delivery, without history of taking antibiotics and gastrointestinal disorders on the cell viability of two types of cells with different growth features including colon cancer cells (Caco-2) and Unrestricted Somatic Stem Cells (USSCs). After fecal sampling, the microbiota was extracted by ethyl acetate. After removing the solvent, the result of extraction was solved in a few volume of DMSO. The effects at concentrations of 2.5 and 5 µg/ ml on the following cells were determined after 72 hours of incubation using MTT assay. The results showed that the cell viability of USSCs was significantly increased as the concentration elevated. While at the same conditions, the viability of Caco-2 cells was decreased at the higher concentration significantly.

Cancer is a main health problem worldwide and the number of cancer patients is increasing annually. Cancer treatment needs new anticancer medicines because of drug resistance. Rosemary is one of the herbal medicines which its anti-cancer effects have been reported. The purpose of the present study was to evaluate rosemary extract effect on HN5 cancer cells viability in comparison with neuronal progenitor cells(NPCs). NPCs were obtained from 17 days pregnant mice by enzymatic digestion method. These cells and HN5 cells were treated with 50, 100, 200 and 500 µg/ml of rosemary extract for 24, 48 and 72 hours. Their viability was measured using MTT assay. Results showed that rosemary extract decreased HN5 cells viability in 100, 200 and 500 µg/ml concentrations which were significant in comparison with NPCs. The extract increased NPCs proliferation rate in 50 and 100 µg/ml concentration and decreased their viability in 500 µg/ml concentration. Rosemary extract can decrease cell viability as a dose and time dependent manner but this effect also depends on the cell type such as its killing effect on cancer cells was more.

Tobacco (Nicotianatabacum L.) is known as a model plant in tissue culture and genetic engineering from the past decades. In this study the effect of ultrasonic waves on viability and disruption of tobacco cells were investigated. For callus induction, tobacco leaf explants cultured on MS medium supplemented with 2,4-D 1.5 mg/l and 1 mg/l Kinetin. The cell suspension culture was initiated by transferring of calli into liquid medium. Then the cell suspensions exposed to ultrasonic wave with different levels of temperature and duration. Cell viability was determined by Trypan blue staining, and structural and ultra-structural disruptions in cell wall were evaluated using optical and scanning electron microscopy (SEM). The results showed that the viability of cells significantly influenced by temperature and duration of ultrasonic wave treatments. The highest cell viability (%76.702) was obtained with 2 min sonication at 25 or 40 °C, while, the highest cell wall disruption were observed at 50° C with 14 min sonication (% 18.733) and at 25° C with 20 min of ultrasonic treatment (% 17.48). Scanning electron microscopy (SEM) was confirmed the formation of different sized pores on cell wall under ultrasound treatment.

ß-carbolines, harmaline and harmine, are major alkaloids present in the seeds of Peganum harmala L. These alkaloids are known as herbal active principals with potential use in pharmaceutical and medicine. To assess the growth inhibitory effect of phyto-alkaloids, harmaline and harmine, on cancer cell lines. P. harmala L.’s alkaloids were extracted by acidic/basic extraction method and identified by two methods, Fourier Transform Infra-Red Spectroscopy (FTIR) and High Performance Liquid Chromatography (HPLC). Two breast cancer cell lines, MDA-MB-231 and Mcf-7, were subjected to different concentrations (1–100 μg.mL-1) of the P. harmala extract at different time courses (24, 48, and 72 hours). Methylthiazol Tetrazolium (MTT) test, half maximal inhibitory concentration (IC50) and morphological changes through optical microscopy were evaluated. In both studied cell lines, the P. harmala extract decreased cell viability in longer time exposure in a dose dependent manner. The more concentrated extract led to higher motility of MDA-MB-231 at 24 hours. Although, Mcf-7 cell line required longer exposure time to reach the same motility. It was observed that 30 μg.mL-1 is the minimum lethal dose that kills approximately 50% of cells at 24 hours for MDA-MB-231 cell line (IC50). IC50 for Mcf-7 was calculated 40 μg.mL-1 and 25 μg.mL-1 48 and 72 hours after being exposed against harmala’s extract, respectively. The morphological observation confirmed the apoptosis nature of P. harmala on cells as their membrane kept intact and no membrane permeabilization was observed. The results revealed that the P. harmala extracts significantly decreased the growth rate and cell survival of cancer cell lines. The extract induced cell death regarding natural cell growth rate. MDA-MB-231 cell line naturally has a higher growth rate than Mcf-7 cell line, thus higher growth inhibition of MDA-MB-231 cell line by the P. harmala extract was confirmed.