جستجوی مقالات مرتبط با کلیدواژه "staphylococcus aureus" در نشریات گروه "شیمی"
تکرار جستجوی کلیدواژه «staphylococcus aureus» در نشریات گروه «علوم پایه»-
نشریه کیفیت و ماندگاری تولیدات کشاورزی و مواد غذایی، سال چهارم شماره 2 (پیاپی 14، پاییز 1403)، صص 51 -59
پنیر سنتی از محبوب ترین انواع پنیر در مناطق غربی ایران است. آلودگی پنیر به میکروارگانیسم های بیماری زا می تواند سلامت انسان را به خطر انداخته و موجب ضررهای اقتصادی قابل توجهی گردد. هدف از مطالعه حاضر، بررسی حضور باکتری های اشریشیاکلی و استافیلوکوکوس اورئوس و تعیین الگوی مقاومت آنتی بیوتیکی آن ها در پنیرهای سنتی تولید شده در شهرستان جلفا بود. 60 نمونه پنیر سنتی به صورت تصادفی از مغازه های شهرستان جلفا، جمع آوری شد. برای بررسی حضور میکروارگانیسم های موردنظر طبق استانداردهای ملی موادغذایی، از محیط گیولیتی کانتونی براث، بردپارکر، بلاد آگار و مولر هینتون آگار و همچنین برای تایید گونه باکتری از تست های تشخیصی و بیوشیمیایی استفاده شد. الگوی مقاومت آنتی بیوتیکی نسبت به شش آنتی بیوتیک تعیین شد. نتایج نشان داد که تمامی نمونه ها آلوده به استافیلوکوکوس اورئوس و اشریشیاکلی بودند. همچنین، در جدایه های استافیلوکوکوس اورئوس، بیشترین حساسیت آنتی بیوتیکی مربوط به آمیکاسین با 67/66 درصد و مقاومت دارویی به سفکسیم با 75 درصد بود. در مورد اشریشیاکلی، بیشترین حساسیت آنتی بیوتیکی مربوط به آمیکاسین با 100 درصد و بیشترین میزان فراوانی مقاومت در این باکتری به آزیترومایسین با 25 درصد، گزارش شد. نتایج مطالعه حاضر نشان داد که وضعیت پنیرهای سنتی در شهرستان جلفا بسیار نامطلوب بوده و آگاهی صحیح در زمینه استفاده از فرآورده های سنتی در پیشگیری از بروز مشکلات بهداشتی و درمانی ازجمله بیماری های گوارشی بسیار ضروری می باشد.
کلید واژگان: استافیلوکوکوس اورئوس, اشریشیاکلی, مقاومت دارویی, پنیر سنتی, جلفاJournal of Quality and Durability of Agricultural and Food Products, Volume:4 Issue: 2, 2024, PP 51 -59Traditional cheese is one of the most popular types of cheese in the western regions of Iran. Contamination of cheese with pathogenic microorganisms can pose a risk to human health and result in significant economic losses. The aim of the present study was to investigate the presence of Escherichia coli and Staphylococcus aureus and to determine their antibiotic resistance patterns in traditional cheeses produced in Jolfa County. 60 samples of traditional cheese were randomly collected from shops in Jolfa County. To examine the presence of the targeted microorganisms according to national food standards, the study utilized Giolitti-Cantoni broth, Baird-Parker agar, blood agar, and Mueller-Hinton agar, as well as diagnostic and biochemical tests for bacterial species confirmation. The antibiotic resistance pattern against six antibiotics was determined. The results indicated that all samples were contaminated with Staphylococcus aureus and Escherichia coli. Additionally, among the Staphylococcus aureus isolates, the highest antibiotic sensitivity was to amikacin at 66.67%, while the highest resistance was to cefixime at 75%. In case of Escherichia coli, the highest antibiotic sensitivity was also to amikacin at 100%, and the highest resistance frequency for this bacterium was reported for azithromycin at 25%. The results of the present study showed that the condition of traditional cheeses in Jolfa County is highly undesirable, and awareness regarding the proper use of traditional products is essential in preventing health and treatment issues, including gastrointestinal diseases.
Keywords: Staphylococcus Aureus, Escherichia Coli, Antibiotic Resistance, Traditional Cheese, Jolfa -
Bacteria such as Staphylococcus aureus and Escherichia coli are the most common causes of foodborne illness. Candida tropicalis is also found in the faeces of chronic diarrhoea patients. Biofilms can form on the surfaces of living or inanimate objects. Biofilm formation contributes to various infections and makes their eradication difficult. The Nephelium mutabile plant is known to have active substances that are antibacterial, anti-oxidative, anti-inflammatory, anti-mutagenic, and anti-carcinogenic. The objective of this study is to determine the effectiveness of Nephelium mutabile leaf extract in inhibiting biofilms formed by Staphylococcus aureus, Escherichia coli, and Candida tropicalis. This study used a Completely Randomized Design [CRD] with two sample groups: the sample group with the addition of Nephelium mutabile extract before biofilm formation and the group sample without any treatment. The concentration of Nephelium mutabileleaf extract for the biofilm inhibition test was 25%. Distilled water is used for negative control. Nephelium mutabile extract was most effective in inhibiting the formation of a Candida tropicalis biofilm [92.24%], and the lowest effectiveness inhibited the formation of Staphylococcus aureus biofilm [35.76%]. Nephelium mutabile leaf extract has the potential to be an antibiofilm for bacteria and fungi [Staphylococcus aureus, Escherichia coli, and Candida tropicalis].Keywords: Antibiofilm, Staphylococcus Aureus, Escherichia Coli, Candida Tropicalis, Nephelium Mutabile
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Nanotechnology-based antibiotic synthesis is one of the most crucial contemporary strategies for preventing antibiotic resistance. Synthesis of nano sulfadiazine antibiotic was nanoscale made by using standard sulfadiazine in this study, Physically, without using any chemicals. The resulting nanocomposite was examined using XRD, EDX, and SEM methods, and their characteristics were contrasted with those of nano sulfadiazine, whose average crystal size was 48.32 nm. The ability of nano sulfadiazine to prevent bacteria growth was examined by measuring the minimum inhibitory concentration of two species of bacteria using an ELISA technique; it was compared to regular sulfadiazine particles. The results of the broth microdilution method with standard sulfadiazine gradient (concentration) ranges of 8-1024μg/ml show the MIC ranging 64-128μg/ml among five MDR P. aeruginosa isolates and five MDR S. aureus isolates. While the results of Nano-sulfadiazine MIC ranged from 16-32μg/ml for P. aeruginosa, isolates and 32μg/ml for S. aureus isolates.Keywords: EDX, Nano-Antimicrobial, Pseudomonas Aeruginosa, SEM, Staphylococcus Aureus
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Methicillin-resistant Staphylococcus aureus (MRSA) poses an increasingly alarming threat to global public health, characterized by its escalating antibiotic resistance and heightened virulence. This dire situation necessitates the exploration of novel and hitherto uncharted targets for effective pathogen control. The primary objective of our current investigation is to identify the most promising riboswitch as a druggable target and assess its potential inhibition through both small molecules and antisense oligonucleotides (ASO) in silico. I conducted an exhaustive search for the most plausible druggable riboswitch using the RiboScan tool, subsequently subjecting its sequence to rigorous docking studies with ten distinct resveratrol derivatives through the PatchDock algorithm. In addition, we harnessed the power of ASO by designing and hybridizing five ASO sequences to silence the identified riboswitch target effectively. This comprehensive analysis pinpointed the glmS riboswitch as the most promising candidate for targeted intervention. Molecular docking results uncovered the exceptional inhibitory potential of resveratrol derivatives, with particular emphasis on ZINC000100827960, which emerged as a lead natural inhibitor. Furthermore, the ASO sequences, meticulously designed for their intended purpose, exhibited compelling efficacy in terms of hybrid free energy, RNA-RNA docking interactions, and a remarkably low probability of causing off-target effects within the human transcriptome. Taken together, our findings underscore the significant promise of both resveratrol derivatives and the ASO candidates as potent inhibitors of the glmS riboswitch in MRSA. These results warrant not only further consideration, but also rigorous experimental validation to pave the way for innovative strategies in combating the menace posed by MRSA.Keywords: Staphylococcus aureus, Methicillin resistant, riboswitch, Antisense oligonucleotides, Resveratrol, Molecular docking
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نشریه کیفیت و ماندگاری تولیدات کشاورزی و مواد غذایی، سال سوم شماره 1 (پیاپی 9، تابستان 1402)، صص 13 -20پیشرفت روزافزون مقاومت آنتی بیوتیکی باکتری ها زمینه را برای جایگزین کردن عوامل ضدمیکروبی با منشا گیاهی و با عوارض جانبی کمتر فراهم نموده است. این پژوهش نوعی مطالعه آزمایشگاهی بوده که با هدف تعیین اثر ضدباکتریایی گیاه جوزهندی بر جدایه های استافیلوکوکوس اوریوس مولد بتالاکتاماز وسیع الطیف انجام گرفت. عصاره متانولی گیاه به روش ماسراسیون تهیه گردید. عصاره با کاغذ واتمن شماره یک فیلتر شده و توسط سیستم تقطیر در خلا دوار تغلیظ و خشک شد. غلظت های مختلف 80، 40، 20، 10، 5، 5/2، 25/1 و 625/0 از عصاره در حلال دی متیل سولفوکساید و متانول با حجم برابر تهیه گردید. شناسایی جدایه های مولد بتالاکتاماز به روش فنوتیپی با دیسک های آنتی بیوتیکی سفوتاکسیم و دیسک ترکیبی سفوتاکسیم و کلاولونیک اسید انجام شد. فعالیت ضدباکتریایی بر علیه 40 ایزوله از باکتری های مولد بتالاکتاماز، به روش انتشار چاهک بررسی شد. پس از انکوباسیون به مدت 24 ساعت در دمای 37 درجه سانتی گراد میزان حساسیت باکتری ها، با اندازه گیری قطر هاله بازدارندگی از رشد تعیین شد. بر اساس نتایج حاصله، از 60 باکتری استافیلوکوکوس اوریوس 67 درصد از جدایه ها مولد بتالاکتاماز بودند. کلیه ایزوله های استافیلوکوکوس اوریوس به عصاره گیاه جوزهندی حساسیت نشان دادند و میانگین حداقل غلظت بازدارندگی از رشد نسبت به استافیلوکوکوس مولد بتالاکتاماز، 10 میلی گرم بر میلی لیتر بود. به دلیل افزایش روزافزون مقاومت آنتی بیوتیکی، به نظر می رسد که بتوان از عصاره ی گیاه جوز بر علیه سوش های استافیلوکوکوس اوریوس مقاوم به بتالاکتام در کنترل عفونت ها استفاده کرد و در این راستا، جداسازی و شناسایی مواد موثره عصاره گیاهی پیشنهاد می گردد.کلید واژگان: استافیلوکوکوس اورئوس, بتالاکتاماز, جوز هندی, فعالیت ضدباکتریاییJournal of Quality and Durability of Agricultural and Food Products, Volume:3 Issue: 1, 2023, PP 13 -20The increasing development of antibiotic resistance of bacteria has provided the basis for replacing antimicrobial agents with plant origin and with less side effects. This research is a type of laboratory study that was conducted with the aim of determining the antibacterial effect of Myristica fragrans on staphylococcus isolates beta lactamase producing antibiotics. The methanol extract of the plant was prepared by maceration method. The extract was filtered with Whatman No.1 paper and concentrated by rotary evaporator system. Different concentrations of 200, 100, 80, 40, 20, 10, 5, 2.5, 1.25, 0.625 of the extract were prepared in DMSO: Methanol (1:1v/v) solvent. Identification of beta-lactamase producing isolates was done by phenotypic method with cefotaxime antibiotic discs and cefotaxime / clavulanic acid combined disc. Antibacterial activity against 40 isolates of beta-lactamase-producing isolates was investigated by agar wells diffusion method. After incubation for 24 hours at 37°C, the sensitivity of bacteria was determined by measuring the diameter of the growth inhibition zone. Based on the results, out of 60 Staphylococcus aureus bacteria, 67% of isolates were beta-lactamase producers, respectively. All isolates of Staphylococcus aureus showed sensitivity to Myristica fragrans extract, and the average of minimum growth inhibition concentration to beta-lactamase-producing Staphylococcus aureus was 10 mg/ml. Due to increasing antibiotic resistance, it seems that Myristica fragrans extract can be used against beta-lactam-resistant Staphylococcus aureus strains in controlling infections, and in this regard, isolation and identification of the effective substances of the plant extract it is suggested.Keywords: Staphylococcus aureus, Beta-lactamase, Myristica fragrans, Antibacterial activity
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The spread of incurable diseases, especially infectious diseases caused by antibiotic-resistant bacteria and certain cancers, has become a serious public health concern. Consequently, the search for potent drug scaffolds has played an essential role in drug lead discovery. The multicomponent reaction (MCR) offers a novel method for efficient synthesis. It is rapidly evolving and is important for the discovery of novel molecules. We synthesized four dihydropyrimidinone (DHPM) derivatives with the one-pot MCR method, obtaining compounds 1-4. According to the NMR spectra analyses, compound 3 is a new derivative. In this experiment, we optimized the pH of the process. Based on the results, 1-4 had yields of 66.6, 72.9, 35.9, and 69.0%, respectively, at a pH of 4. In contrast, all yields significantly rose by 79.4, 91.9, 81.7, and 84.0% at pH 5. A pH of 5 was therefore advantageous for getting a high yield from these reactions. Compound 1 showed a significant inhibition against E. coli with an MIC value of 12.5 µg/mL with moderate activity against the breast cancer cell lines T47D and 4T1. Compound 3 was the most potent against S. aureus, with an MIC value of 25 µg/mL.Keywords: Dihydropyrimidinone derivatives, Bioactivity, E. coli, Staphylococcus aureus, T47D, 4T1 cell lines
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Staphylococcus Aureus is an extremely dangerous infectious pathogen in the healthcare and community setting. Discovery of the right chemotherapies to treat this infection has been difficult due to the high toxicity associated with some of the most effective drugs. Computational chemistry is helping to identify potential effective drugs to treat this infection. In this study molecular docking was utilized to examine the effects of 3 different compounds on Staphylococcus aureus and HTH3E. The structure of the ligands was drawn in Chemdraw software and the molecular docking was carried out using Pyrx computational tool. Visualizations of the docking interactions with the target active site were generated via Discovery Studio. HTH3E showed the lowest binding affinity with a score of -27.105 kcal/mol. The results demonstrate that (3-amino-5-hydroxy-2-methyl-1H-pyrrol-1-yl)(5-hydroxy-1H-1λ6-thiophen-3-yl)methyl carbamic acid is a promising lead and therefore further study of this compound is warranted. The newly designed compound was also subjected to molecular dynamics study and was found to be stable and firmly fixed in the binding pocket of the receptor.
Keywords: Molecular docking, Staphylococcus aureus, MgrA, ligand -
نشریه کیفیت و ماندگاری تولیدات کشاورزی و مواد غذایی، سال دوم شماره 4 (پیاپی 8، بهار 1402)، صص 55 -63زمینه و هدف
باکتریهای پاتوژن موجود در مواد غذایی عامل بسیاری از عفونتها و مسمومیتهای غذایی هستند. شیوع آلودگیهای باکتریایی در کشورهای در حال توسعه بالا میباشد و استافیلوکوکوس اوریوس، سالمونلا و اشرشیاکلای بالاترین میزان آلودگیهای غذایی را به خود اختصاص دادهاند؛ لذا هدف از مطالعه حاضر بررسی شیوع اشرشیاکلای، استافیلوکوکوس اوریوس و سالمونلا در آب، آبمیوه و بستنیهای سنتی عرضه شده در شهرستان قم، ایران میباشد.
روش بررسیدر مطالعه حاضر تعداد 300 نمونه شامل 100 نمونه آب، 100 نمونه آبمیوه و 100 نمونه بستنیسنتی از مراکز عرضه، نمونه گیری و به آزمایشگاه بهداشت مواد غذایی دانشگاه آزاد شهرکرد منتقل شد. از نرم افزار آماری SPSS نسخه 26 و آزمون آماری کای حهت آنالیز داده ها استفاده شد. سطح معنیداری (P<00/05) در نظر گرفته شد.
یافته هانتایج نشان داد که از 300 نمونه مورد مطالعه، 170 نمونه به میکروارگانیسمهای پاتوژن آلوده بودند. به این ترتیب میزان آلودگی به اشرشیاکلای، استافیلوکوکوس اوریوس و سالمونلا در آب، 1، 16 و 2 نمونه، در آبمیوهسنتی 8، 46 و 26 نمونه و در بستنی سنتی 8، 59 و 28 نمونه آلودگی وجود داشت.
نتیجهگیریبا توجه به نتایج حاضر، ضرورت دارد که از مصرف آبمیوه سنتی و بستنی سنتی در مراکزی که وضعیت بهداشت نامطلوبی دارند خودداری شده و از آبهای تصفیهشده استفاده شود و در صورت عدم وجود آب تصفیه شدهقبل از مصرف آب جوشیده شود تا از ابتلا به بیماریهای منتقلشونده از مواد غذایی جلوگیری به عمل آید.
کلید واژگان: استافیلوکوکوس اورئوس, اشرشیاکلای, سالمونلا, مواد غذایی, آلودگی باکتریاییJournal of Quality and Durability of Agricultural and Food Products, Volume:2 Issue: 4, 2023, PP 55 -63Background and purposepathogenic bacteria in food are the cause of many infections and food poisoning. The prevalence of bacterial contamination is high in developing countries, and Staphylococcus aureus, Salmonella and Escherichia coli account for the highest amount of food contamination; Therefore, the aim of this study is to investigate the prevalence of Escherichia coli, Staphylococcus aureus and Salmonella in water, fruit juice and traditional ice creams offered in Qom city, Iran.
Research methodIn the present study, 300 samples, including 100 water samples, 100 fruit juice samples, and 100 traditional ice cream samples, were taken from supply centers, sampled and transferred to the food hygiene laboratory of Shahrekord Azad University. SPSS version 26 statistical software and Kai-Hat statistical test were used for data analysis. A significant level (P<00.05) was considered.
FindingsThe results showed that out of 300 studied samples, 170 samples were infected with pathogenic microorganisms. In this way, the amount of Escherichia coli, Staphylococcus aureus and Salmonella contamination in water was 1, 16 and 2 samples, in traditional fruit juice 8, 46 and 26 samples and in traditional ice cream 8, 59 and 28 samples.
ConclusionAccording to the present results, it is necessary to refrain from consuming traditional fruit juice and traditional ice cream in centers that have unfavorable health conditions and to use purified water, and if there is no purified water before Boiled water should be consumed to prevent food-borne diseases.
Keywords: Staphylococcus aureus, Escherichia coli, Salmonella, food, biological food -
Rapid augmentation in the prevalence of multidrug-resistant (MDR) Staphylococcus aureus is a worldwide threat. Advising newer antibiotics may fail to reduce the chances of the emergence of newer drug-resistant Staphylococcus aureus. Very little shreds of evidence can be found to treat clinical MDR Staphylococcus aureus with biogenic silver nanoparticles (AgNPs) in West Bengal. To prepare AgNPs biogenically using aqueous tulsi leaf extract (TLE) and also to assess its antibacterial effect upon clinical MDR Staphylococcus aureus, biogenic synthesis of the AgNPs using aqueous TLE was done, characterized those with UV-Vis Spectrophotometer, dynamic light scattering, field emission scanning electron microscopy, Fourier transform infrared spectroscopy, and evaluated the antibacterial activity against the clinical MDR Staphylococcus aureus. ANOVA followed by LSD post hoc test was used to test the differences between the OD (optical density) of different experimental sets. The biosynthesized AgNPs were spherical, monodispersed, and of smaller size (9-23 nm) with the involvement of eugenol, quercetin, and oleanolic acid present in the tulsi leaf. A significant change in OD was observed in AgNPs (prepared using TLE) treated broth compared to only tulsi leaf extract treated culture. There was a significant similarity between the efficacies of AgNPs and clindamycin (P < 0.05). Our findings propose that AgNPs synthesized using TLE were fast and efficient to ameliorate the bacterial growth, which may be used as a potent antibacterial agent for the treatment of clinical MDR Staphylococcus aureus infection in near future.Keywords: Ag Nanoparticles, Biogenic, Clindamycin, MDR, MRSA, Staphylococcus aureus, Tulsi
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نشریه کیفیت و ماندگاری تولیدات کشاورزی و مواد غذایی، سال دوم شماره 1 (پیاپی 5، تابستان 1401)، صص 35 -46پنیر فراورده ای است متشکل از چربی و پروتیین شیر، به همراه کلسیم و فسفری که به صور مختلف با پروتیین شیر ترکیب شده اند. با توجه به اهمیت مصرف پنیر در بین مردم ایران و با توجه به این که این محصول یکی از مهمترین فراورده های لبنی است و براساس آمارهای موجود حدود 20 درصد شیر تولیدی کشور در بخش صنایع لبنی تبدیل به پنیر می شود که از این مقدار سهم تولید پنیر سنتی در حدود 80 درصد می باشد، هدف از این تحقیق، ارزیابی میزان آلودگی پنیرهای سنتی عرضه شده در مناطق مختلف شهرستان کرج بود. مطالعه حاضر از نوع توصیفی- مقطعی، در تابستان 1398 با نمونه برداری از بخش های مختلف شمال، غرب، شرق، جنوب و مرکز شهرستان کرج انجام شد. چهار نوع پنیر فله لیقوان تبریز، لاکتیکی، سفید و گچی در پنج منطقه جغرافیایی با چهارتکرار، از نظر وجود باکتری استافیلوکوکوس اوریوس مورد بررسی قرار گرفت. مطالعه حاضر نشان از آلودگی پنیرهای سنتی عرضه شده در شهرستان کرج و مناطق مختلف آن دارد، اگرچه که میزان آلودگی در غرب شهرستان، از سایر مناطق بیشتر بوده است. در بین مناطق، منطقه شرق با کمترین میزان آلودگی در بین سایر مناطق و وجود آلودگی تنها در پنیر گچی، به عنوان کم خطر ترین منطقه شناسایی شد. در مقابل وجود آلودگی بالا در هر چهار نوع پنیر مورد مطالعه در غرب شهرستان کرج، این بخش از شهرستان کرج را بر اساس نتایج این تحقیق به عنوان پرخطرترین منطقه در مصرف پنیرهای سنتی معرفی کرد.کلید واژگان: آلودگی میکروبی, استافیلوکوکوس اورئوس, پنیر, مناطق, کرجJournal of Quality and Durability of Agricultural and Food Products, Volume:2 Issue: 1, 2022, PP 35 -46Cheese is a product consisting of fat and milk protein, along with calcium and phosphorus that are combined with milk protein in various ways. Due to the importance of cheese consumption among the people of Iran and considering that this product is one of the most important dairy products and according to available statistics, about 20% of the milk produced in the dairy industry is converted into cheese, of which the share of production Traditional cheese is about 80%. The purpose of this study was to evaluate the level of contamination of traditional cheeses offered in different areas of Karaj. The present descriptive cross-sectional study was conducted in the summer of 1398 by sampling different parts of the north, west, east, south and center of Karaj . Four types of bulk cheeses of Tabriz Ligvan, lactic, white and gypsum in five geographical areas with three replications were examined for the presence of Staphylococcus aureus. The results shows the contamination of traditional cheeses and its different areas, although the rate of contamination in the west was higher than other areas. Among the regions, the eastern region with the lowest level of pollution among other regions and the presence of contamination only in gypsum cheese, was identified as the least dangerous region. In contrast to the existence of high pollution in all four types of cheese studied in the west. based on the results, this part of Karaj was introduced as the most dangerous area in the consumption of traditional cheeses.Keywords: Microbial Pollution, Staphylococcus aureus, Cheese, Regions, Karaj
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بررسی فعالیت ضد میکروبی پپرین جدا شده از فلفل سیاه بر روی استافیلوکوکوس اورئوس جدا شده از حلق و بینینشریه کیفیت و ماندگاری تولیدات کشاورزی و مواد غذایی، سال دوم شماره 1 (پیاپی 5، تابستان 1401)، صص 67 -73به دلیل مقاومت روزافزون باکتری های بیماری زا به آنتی بیوتیک های رایج، محققین در پی یافتن عوامل ضد میکروبی با منشاء گیاهی به عنوان داروهای جایگزین می باشند. هدف از این مطالعه بررسی فعالیت ضد میکروبی پپرین جدا شده از فلفل سیاه بر روی استافیلوکوکوس اوریوس جدا شده از حلق و بینی است. نمونه برداری از نواحی حلق و بینی بیماران انجام و تعداد 9 سویه استافیلوکوکوس اوریوس جدا و خالص سازی گردید. حداقل غلظت بازدارندگی (MIC) و حداقل غلظت کشندگی (MBC) در شش غلظت با روش رقیق سازی در محیط مایع بر روی باکتری تعیین شده حساسیت سویه ها به چند آنتی بیوتیک با روش استاندارد دیسک دیفیوژن کربی- بایر بیوگرام ارزیابی گردید. نتایج حاصل از این مطالعه نشان داد که کمترین غلظت مهارکنندگی برابر با 8 میکروگرم بر میلی لیتر بوده است که یک سویه مهار شده است در حالی که بیشترین غلظت 512 میکروگرم / میلی گرم بوده که یک سویه مهار شده است. استافیلوکوکوس اوریوس جدا شده بیشترین مقاومت به تری متوپریم (8/88%)، آمپی سیلین (7/77%)، تتراسیکلین (7/77%)، اریترومایسین(6/66%) و سفتازیدیم (2/22%) بوده است. نتایج حاصل از این مطالعه نشان داد که پیپرین موجود در فلفل سیاه دارای اثر مهاری بسیار قوی بر روی باکتری های استافیلوکوکوس اوریوس جدا شده از حلق و بینی دارد.کلید واژگان: پیپرین, استافیلوکوکوس اورئوس, الگوی مقاومت آنتی بیوتیکیJournal of Quality and Durability of Agricultural and Food Products, Volume:2 Issue: 1, 2022, PP 67 -73Due to the increasing resistance of pathogenic bacteria to common antibiotics, researchers are looking for antimicrobial agents of plant origin as alternative drugs. The aim of this study is to investigate the antimicrobial activity of peperin isolated from black pepper on Staphylococcus aureus isolated from the nose and throat. Sampling was done from the pharynx and nose of the patients and 9 strains of Staphylococcus aureus were isolated and purified. The minimum inhibitory concentration (MIC) and the minimum lethal concentration (MBC) in six concentrations were evaluated by dilution method in liquid medium on the determined bacteria. The results of this study showed that the lowest inhibitory concentration was equal to 8 μg/ml, which inhibited one strain, while the highest concentration was 512 μg/mg, which inhibited one strain. Staphylococcus aureus isolated the most It was resistant to trimethoprim (88.8%), ampicillin (77.7%), tetracycline (77.7%), erythromycin (66.6%) and ceftazidime (22.2%). The results of this study showed that piperine present in black pepper has a very strong inhibitory effect on Staphylococcus aureus bacteria isolated from the nose and throat.Keywords: piperine, Staphylococcus aureus, Antibiotic resistance pattern
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نشریه کیفیت و ماندگاری تولیدات کشاورزی و مواد غذایی، سال دوم شماره 1 (پیاپی 5، تابستان 1401)، صص 14 -23آلودگی و فساد مواد غذایی از بسیاری لحاظ مانند سلامتی و اقتصادی حایز اهمیت می باشد. شناسایی آلودگی های مواد غذایی می تواند کمک قابل توجهی به بهبود فرآیندهای تولید و توزیع مواد غذایی نموده و از هدر رفت بسیاری از منابع جلوگیری نماید. در ایران نیز طی دوره های زمانی پیوسته تحقیقات بسیاری در این زمینه انجام شده است که بررسی دقیق این پژوهش ها می تواند اطلاعات ارزشمندی را فراهم نماید. در این مقاله تلاش شده است تا به بررسی مختصر از انواع آلودگی های مواد غذایی در دسته های لبنی، گوشتی و شیرینی پزی سال های اخیر در ایران پرداخته و گزارشی از نوع کتاب سنجی از اسناد منتشر شده در پایگاه اسکوپوس بدون محدودیت زمانی ارایه گردد. نتایج بررسی ها مشخص نمود که احتمالا باکتری های اشریشیا کلی و استافیلوکوکوس اوریوس در این کشور نسب به سایر باکتری ها بیشتر مطرح باشند. نسبت کم تعداد اسناد مروری به مقالات و همچنین عدم انتشار هیچ کتابی در این پایگاه می تواند مورد توجه محققین جهت گردآوری، جمع بندی و نتیجه گیری این اطلاعات باشد که قطعا مفید خواهد بود.کلید واژگان: فساد موادغذایی, آلودگی باکتریایی, اشریشیا کلی, استافیلوکوکوس اورئوسJournal of Quality and Durability of Agricultural and Food Products, Volume:2 Issue: 1, 2022, PP 14 -23Contamination and spoilage of food are important in many ways, such as health and the economy. Identification of food contamination can significantly help to improve food production and distribution processes and prevent the wastage of many resources. In Iran, many researches have been carried out in this field during continuous periods of time, and a careful examination of these researches can provide valuable information. In this article, an attempt has been made to briefly review the types of food contamination in the dairy, meat, and confectionery categories in recent years in Iran and provide a bibliometric report of the documents published in the Scopus database without a time limit. The results of the investigations revealed that Escherichia coli and Staphylococcus aureus bacteria are probably more important than other bacteria in this country. The low ratio of the number of review documents to articles and also the lack of publication of no book in this database can be of interest to researchers to collect, summarize and conclude this information, which will definitely be useful.Keywords: food spoilage, bacterial contamination, Escherichia coli, Staphylococcus aureus
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In this work, Enhancement activity of nanocomposite metal Oxide by laser for medical application, nanocomposite metal Oxide we prepared by using a simple Sol-Gel method that used to synthesize and measure (〖TiO〗_2, ZnO/〖TiO〗_2and Cu: ZnO/〖TiO〗_2). X-ray diffraction (XRD) was used to describe the crystalline structures, Cu2O spikes were observed (26.393 °), and the amplitude of these peaks became high with a copper concentration that explained a higher Cu2O mass fraction was provide from the copper load. The pictures from the FTSEM showed that most particles are spherical. The effect of heat treatment and laser on the particle size was substantial as well. The chemical bonds and the functional groups of TiO2 NPs were analyzed using Fourier Transform Infrared Spectroscopy (FTIR) within a 400-4000 cm-1 wavenumber range. The solid and broad O-H stretching peaks, C-H bending, N-H bending, and C-Cl stretching chemical bonds were obtained. The photocatalytic activity of (〖TiO〗_2, ZnO/〖TiO〗_2and Cu: ZnO/〖TiO〗_2) were then evaluated under laser rays. ZnO/TiO2 were calcination at 500 °C showed the highest photoactivity compared to that calcination at 400, 600, and 700°C That has the top activity than Cu: ZnO/TiO2 and titanium oxide consequently The antibacterial activity of the prepared specimens was indicated by the Kirby-Bauer disc method that Enhancement photocatalytic activity by laser. ZnO/TiO2 NPs showed a very efficient antibacterial action against Staphylococcus aureus strains extraordinary antibacterial efficacy against S. aureus bacterium strainsKeywords: nanocomposites metal Oxide, Photocatalytic activity, Sol-Gel, Staphylococcus aureus
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Iranian Journal of Chemistry and Chemical Engineering, Volume:40 Issue: 3, May-Jun 2021, PP 866 -871
This paper deals with the study on the antibacterial activity of TiO2-doped Ag prepared from radiophotography wastewater. The antibacterial agent was prepared by reduction of Ag(I) in the radiophotography wastewater over TiO2 photocatalyst under UV light irradiation and characterized by EDS, XRD, SRUV, and TEM machines. The antibacterial activity in inhibiting the growth of Staphylococcus aureus was examined by counting the number of viable bacterial colonies using the TPC method. The result shows that Ag doping on TiO2 as TiO2-Ag can shift its absorption into the visible region. TiO2-Ag assigns better antibacterial activity compared to TiO2 under visible light irradiation. The efficiency of the antibacterial activity is found to be influenced by Ag loaded in the TiO2, irradiation time, and the antibacterial agent dose. The highest antibacterial activity is achieved by 100 mg/L of TiO2-Ag (2) under 3 h irradiation by visible light.
Keywords: TiO2-Ag, Radiophotography wastewater, Photoreduction, Silver ion, Inhibition, Staphylococcus aureus -
Groundnut shells (GSs) are abundant renewable by-products which have been underexploited for potential applications. Therefore, this paper reports the bioactive potential of groundnut shell extracts (GSEs) against Staphylococcus aureus and Pseudomonas aeruginosa. The GSs were ground into powder form and subjected to extraction using ethanol, ethyl acetate, and a mixture of ethanol and ethyl acetate using an electrical shaker for 6 h and 12 h; and subsequently centrifuged at 2000 rpm for 20 min. The GSEs were then qualitatively screened for phenol, quinone, saponin tannins, and flavonoids using the standard procedures. More so, antibacterial activities of these GSEs against P. aeruginosa (ATCC 29953) and S. aureus (ATCC 25923) were tested using Agar well diffusion method on Mueller-Hinton agar (MHA). Therefore, the preliminary phytochemical screening reviewed the presence of saponin, tannin, flavonoid, quinone, and phenol. And the investigation of the antibacterial activities against Staphylococcus aureus and Pseudomonas aeruginosa demonstrated that S. aeureus was more sensitive to attack by the EtOH derived GSEs; whereas, P. aeruginosa was readily affected by the EtOAc GSEs. Generally, P. aeruginosa was more inhibited by these GSEs even at the lower concentrations of 25 and 12.5 mg/ mL; especially with the EtOH + EtOAc and EtOAc derived GSEs. EtOH + EtOAc GSE has potential of enhancing these bacterial inhibitionsKeywords: Sustainable feed-stock, Bioactivity, Staphylococcus aureus, Pseudomonas aeruginosa
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Fe3O4 magnetic structure was synthesized with co-precipitation method. Surface of magnetic core was modified with hydrophobic BMIM[PF6] ionic liquid. The samples became antibacterial by loading gold, copper and silver nanoparticles and denoted as Fe3O4/IL/X (X=Ag, Au, Cu). X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), thermal gravimetric analysis (TGA), Atomic absorption spectroscopy (AAS), Fourier transform infrared (FTIR) and vibration sample magnetometer (VSM) technics were applied for catalysts characterization, metal concentration analysis and morphology monitoring. Modified nanostructures were used for inactivation of Escherichia coli as the gram negative and Staphylococcus aureus as the gram positive of bacteria. Transmition electron micrscopy (TEM) images indicated that highest bacteria cell walls destruction is achieved when the surface of the magnetic nanostructure is coated with gold particles. Hydrogen bonds between cell wall and ionic liquid and gradual release of metals from Fe3O4/IL surface facilitate the metals arrive to outer layer of bacteria. Minimum inhibitory concentration (MIC) study approved the positive effect of ionic liquid.Keywords: Magnetic nanoparticles, Ionic Liquids, Antibacterial Activity, Escherichia coli, Staphylococcus aureus
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Santaloïdes afzelii (R.Br. ex Planch.) Schellenb (Connaraceae) is used in Africa as an analgesic, aphrodisiac and in the treatment of stomachaches, muscular pains and in medico magic rites. The present study aims to identify the bioactive organic compounds from the bark and seeds of Santaloïdes afzelii collected in Korhogo in the north of Côte d’Ivoire. Microbiological testing againts Staphylococcus aureus of the hydro ethanolic crude extracts of the bark and seeds showed low activities compared to the activities of oxacillin and cefoxitin. The chemical investigations of these extracts led to the identification of gallic acid, epi-catechin and dimers of procyanidin.Keywords: Connaraceae, Dimers procyanidins, epi-Catechin, Gallic acid, Santaloïdes afzelii, Staphylococcus aureus
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The review article summarizes the applications of silver nanoparticles for diverse sectors. Over the decades, nanoparticles used as dignified metals such as silver exhibited distinctive characteristics basically correlated to chemical, physical and biological property of counterparts having bulkiness. Numerous studies reported that Nanoparticles of about 100nm diameter play a crucial role in widely spread industries due to unique properties including the dimension of small particle, high surface area and quantum confinement and they dispersed without agglomeration. Decade of discoveries clearly established that shape, size and distribution of Silver nanoparticles strongly affect the electromagnetic, optical and catalytic properties, which are often an assortment of changeable synthetic methods and reducing agents with stabilizers. Generation after generation the postulates come forth about properties of silver for the ancient Greeks cook from silver pots and the old adage ‘born with a silver spoon in his mouth’ thus show that eating with a silver spoon was well-known as uncontaminated. Impregnation of metals with silver nanoparticles is a practical way to exploit the microbe aggressive properties of silver at very low cost. The nanoparticles help in targeted delivery of drugs, enhancing bioavailability, sustaining drug or gene effect in target tissues, and enhancing the stability. Implementations of silver partials in medical science and biological science have been noticed from years ago; however alteration with nanotechnology is innovative potential. Over 23% of all nanotechnology based products, diagnostic and therapeutic applications implanted with silver nanoparticles (e.g. In arthritic disease and wound healing, etc.) and widely known for their antifungal, antibacterial, antiviral effect, employed in textile fabrics and added into cosmetic products as antiseptic to overcome skin problems. Thus, Silver nanoparticles (AgNPs) have been urbanized as an advanced artifact in the field of nanotechnology.Keywords: Antimicrobial activity, Biofilm forming, Nanosilver, Silver nanoparticles, Staphylococcus aureus
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Following our interest in reaching for a molded rubber article with possible detergent contact applications, durable silver nanopowder (AgNP) is synthesized by arc discharge, then mixed with varying ratios of ethylene propylene rubber (EPR), affording novel AgNP@EPR nanocomposites. X-ray diffraction (XRD) patterns of AgNP as well as AgNP@EPR show no trace of impurity, while scanning electron microscopy (SEM) indicates an average diameter of 50 nm for the former. Transmission electron microscopy (TEM) images while confirm the SEM results, show quite a few 5 nm AgNP particles lying beside some micro crumbs. Our DC arc discharge technique involves explosion of movable silver anode and static cathode by a current pulse between 5 to 10 A cm-2. A solution blending method is employed for preparation of AgNP@EPR nanocomposites. The AgNP is first dispersed in toluene using an ultrasonic homogenizer, and then thoroughly mixed with EPR in the same solvent whose removal gives nanocomposites of 2, 4, 6 and 8 vol% AgNP in EPR, showing strong antibacterial activity against both Escherichia coli and Staphylococcus aureus.Keywords: Antibacterial Activity, Arc discharge, Escherichia coli, Ethylene propylene rubber (EPR), Silver nanopowder, Staphylococcus aureus
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