جستجوی مقالات مرتبط با کلیدواژه « bio-fertilizer » در نشریات گروه « باغبانی »

Growth-promoting bacteria are among the beneficial soil microorganisms, and by improving the soil quality, they increase the growth and nutritional traits of the plant. Therefore, in order to investigate the effect of growth-promoting bacteria on the leaf surface, specific leaf surface and absorption of nutritional elements of hazelnut seedlings, a factorial experiment was carried out in the form of a randomized complete block design in the field conditions. The main factor was the origin of the seedling (at two levels: Fandoglou and Makesh) and bacterial inoculation (at five levels: P. putida, B. subtilis and E. cloaca, combination of them and Control) with three replications. Twelve seedlings were planted at a distance of 3 × 3 m in holes with dimensions of 50 × 50 × 50 cm on an area of 3240 m2 in the agricultural lands at the neighborhood of Fandoglou forest in Ardabil. The results after four years showed that the inoculated seedlings of both origins were superior in terms of all investigated traits compared to the control (un-inoculated) seedlings. The highest amount of traits was assigned to the seedlings of Fandoglou origin inoculated with the combination of all three bacteria (P. putida, B. subtilis and E. cloaca). The leaf area and specific leaf area were 53.1 and 37.7% more in the inoculated seedling compared to the control, respectively. The concentration of nitrogen, potassium, phosphorous, iron and zinc in the seedling leaf increased by 59.4, 89.5, 23.7, 45.4 and 60.6% compared to the control, respectively. Finally, it can be concluded that the planting of hazelnut seedlings inoculated with growth-stimulating bacteria (combination of the three mentioned bacteria) will have more vegetative growth.

 Heavy metals are one of the most important environmental contaminants, particularly in soil and water sources. Mining and metal mining activities are major factors in soil contamination and generally surface soil around mines contain high amounts of these metals. Lead (Pb) is one of the heavy metals and important pollutant in arid ecosystem. The use of plants to remove contaminated soil or phytoremediation is an economical method. Today, due to increasing the pollution of soil sources and resultant problems, identification of the resistant plant species against soil pollution is essential. Using lead-contaminated soils requires their decontamination and improvement. There are different methods to remove these pollutions, one of them is the use of phytoremediation to remove pollutants from water and soil or to reduce them. Among the plants that as an ornamental plant can have a covering role in the green space and also perform the work of phytoremediation is the ornamental cabbage plants (Brassica oleracea L.). In this regard, this study was conducted with the aim of investigating the effect of lead heavy metals and the use of brown algae (Polycladia indica) and spirulina (Arthrospira platensis) as biofertilizers on ornamental cabbage plants in the direction of plant treatment. In addition, due to the presence of polysaccharide compounds such as carrageenan and alginate in the cell wall, algae (seaweeds) have a higher ability to absorb many heavy metals.

 The experiment was conducted as factorial based on a completely randomized design in 2020 at the research greenhouse of Zanjan University. The studied factors including lead from lead nitrate source Pb(No3)2 with three concentrations (0, 25 and 50 mg/kg) were applied to the potting soil in four replications in two stages with an interval of two weeks. The second factor was included no algae, brown algae (Polycladia indica) and spirulina (Arthrospira platensis), which occurred in four replications. The measured traits included morphological traits: number of leaves, leaf surface index. Wet and dry root weight, and physiological traits included total chlorophyll content, total leaf antioxidant, peroxidase enzyme, glycine betaine, malondialdehyde, and leaf lead and phosphorus content. Data analysis was performed using SAS software and means were compared by LSD method.

 The results of the variance analysis showed that different levels of lead and the application of algae had a significant effect on the number of leaves per plant, leaf area index, weight, and drying of roots, total chlorophyll, and antioxidants. Moreover, the interaction effect was significant for leaf area, root fresh and dry weight, antioxidants, and leaf lead content. The simple effect of lead at p≤0.01 significantly affected glycine betaine, malondialdehyde, and leaf phosphorus. When comparing the average mutual effects of lead and algae application, it was found that the treatment with no use of lead and spirulina algae resulted in the highest weight and dry weight of the root, with an average of 11.19 and 3.625 grams, respectively. Additionally, despite the decrease in dry weight of the root due to increased lead concentration, using algae, especially for ornamental cabbage (Brassica oleracea L.), increased the dry weight of the root. The presence of natural plant hormones, organic substances, carbohydrates, fiber and amino acids in algae accelerates rooting, reduces stress caused by heavy metals and absorbs more water due to the presence of o developed root system. Also according to the results of comparing the amount of leaf lead with increasing lead, algae as an auxiliary factor can reduce the amount of uptake in the plant. Leaf phosphorus was also significant due to the simple effect of lead and algae. The highest amount of phosphorus with an average of 0.56% was observed in spirulina and the lowest with 0.48% was observed in control.

 In general, due to the toxicity of lead metal even in low concentrations, sufficient attention should be paid to the sources of this pollutant entering the environment. In this study, the effective parameters on the uptake of heavy metal lead from the soil by spirulina and brown algae were investigated. The findings of this study indicate that the ornamental cabbage plant is capable of sustaining its growth in the presence of lead and has a high resistance to this heavy metal while simultaneously absorbing it from the soil. Furthermore, the addition of algae as an auxiliary factor can improve the growth of ornamental cabbage under adverse conditions. Therefore, it is recommended that this plant be further examined for its potential to absorb other heavy metals.

The Lippia genus which has over than 200 species and Lippia citriodora L. is medicinally important. In order to evaluate the quantitative and qualitative characteristics and elements of Lippia citriodora L. under the influence of plant growth promoting rhizobacteria (PGPR), vermicompost and biochar, a factorial experiment was conducted in a randomized complete block design with 3 replications in the Research Institute of Forests and Rangelands, Alborz Research Station in field conditions, was implemented in 2017 on one-year seedlings. The  first factor included factors: vermi-compost at three levels (0, 6 and 12 ton/ha), the second factors was biochar  at three levels (0, 5 and 10 ton/ha) and the thirs factor was plant growth promoting rhizobacteria at two levels (non-inoculation and inoculation with combination of Azotobacter chroococcum strain 5, Azospirillum lipoferum strain OF and Pseudomonas putida). Comparison of means demonstrated that the highest essential oil percentage (0.86 %) was acquired from control treatment and the highest essential oil yield with 3.74 kg/ha was observed in treatment 12 ton/ha vermicompost, 10 ton/ha biochar and inoculation with plant growth promoting rhizobacteria. The highest Fe (377.06 ppm) was observed in 12 ton/ha vermicompost, 10 ton/ha biochar and non-inoculation. Generally, application of 6 ton per hectare vermicompost, 10 ton per hectare biochar and PGPRs on Lippia citriodora can improve yield and qualitative traits of the plant through increasing growth, developing root and better absorbing water and nutrients from soil.

Effect of Bacillus subtilis (0, 102, 105, 106, 108 cfu/ml) was studied on saffron (Crocus sativus L.) ecotypes Natanz and Zanjan during 2017-2019 via a factorial experiment based on randomized complete blocks design in three replicates. Number of leaves per corm, average length of leaves, flowers, petals and sepals, stigma fresh and dry weights, fresh and dry flower yield, number of flowers per plot, total phenol and anthocyanin in petals and the amount of nitrogen, phosphorus and potassium were measured. All morphophysiological were significantly affected due to B. subtilis treatment. The highest and the lowest fresh and dry weight of stigma was observed in 108 cfu/ml (41.44 mg and 7.13 mg) and no treated samples (32.91 mg and 6.14 mg), respectively. The maximum stigma fresh and dry weight was observed in Natanz ecotype. The highest amount of nitrogen, phosphorus and potassium was recorded in 108 cfu / ml (5.55 %, 2061ppm 66.75 and 7540.8 ppm, respectively) and the lowest in control (3.25 %, 1314.42 ppm and 4339.8 ppm, respectively). The highest amount of nitrogen and phosphorus was observed in Zanjan ecotype. According to the results, utilizing the B. bacteria in a proper concentration as a bio fertilizer can be considered beneficial towards optimal growth indices in saffron.

In order to investigate the effect of plant growth promoting rhizo-bacteria (PGPRs) and humic acid on some morphological and physiological characteristics of marshmallow (Altheae officinalis L.) a pot experiment was carried out under greenhouse condition at Faculty of Agriculture, University of Birjand, in 2017. The experiment was conducted using a complete randomized blocks design based on factorial arrangement with three replications. First factor was seed inoculation with strains of Pseudomonas fluorescence bacteria at four levels (non- inoculation, inoculation with strains 51, 79 and 163) and second factor was humic acid at four levels (0, 150, 300 and 450 mg/kg soil). Results showed that Pseudomonas fluorescence strains and humic acid had significant effects on morphological and physiological measured traits. The most effects of inoculation created by strain 163 of Pseudomonas fluorescenss bacteria and it increased leaf dry weight, leaf area, leaf chlorophyll index, carotenoids and protoin leaf compare to control. The result also showed that increasing of humic acid concentration up to 150 mg/kg of soil increased, leaf area, leaf chlorophylls index, chlorophyll fluorescence index and protein leaf compare to control. Combination use of Pseudomonas fluorescenss bacteria (strain 163) and humic acid (150 mg/kg soil) had the most effect on increasing of leaf fresh weight, leaf area, chlorophyll a, chlorophyll b, total chlorophyll, carotenoids and phosphor leaf compare to control. Due to these results, application of biological fertilizers containing Pseudomonas fluorescence and humic acid can improve morphological and physiological characteristics of marshmallow.

To determine the effects of Myco-Root biofertilizer and different irrigation levels on the content and essential oil compounds of thyme (Thymus daenensis Celak.), a factorial experiment was conducted in a completely randomized design with eight treatments and three replications in the greenhouse of Plant Production and Genetics department, Faculty of Agriculture, University of Maragheh, Iran, in 2020. The factors included drought stress at four levels of without stress (irrigation at field capacity (FC) as a control), mild (irrigation at 75% FC), moderate (irrigation at 55% FC), and severe (irrigation at 35% FC) stresses and biofertilizer at two levels of inoculation and non-inoculation with Myco-Root. The results demonstrated that the fresh and dry weight of leaves, stem dry weight, total dry weight, leaf relative water content, chlorophylls a and b and total chlorophyll content, and root colonization percentage decreased significantly with increasing the water deficit level. However, the carotenoid content and essential oil percentage showed an increasing trend with increasing drought stress. The highest carotenoid content and essential oil percentage was obtained by using Myco-Root under moderate drought stress. Thymol, carvacrol, p < /em>-cymene, 1,8-cineole, (E)-caryophyllene, borneol, γ-terpinene, and α-pinene were identified as the major constituents of essential oil in the all treatments. The highest content of thymol was obtained under mild drought stress with the Myco-Root application. Furthermore, the highest content of carvacrol was recorded under mild, moderate, and severe drought stresses, respectively with the Myco-Root application. On average, the application of Myco-Root biofertilizer increased the content of thymol, carvacrol, 1,8-cineole, E-caryophyllene, borneol, and α-pinene by 4.19, 48.28, 15.93, 16.33, 14.15, and 3.78%, respectively compared to not using this biofertilizer. Overall, the results showed that some morphological and physiological traits of thyme decreased significantly with increasing the water deficit levels, but the application of Myco-Root biofertilizer could amend drought stress and improve the growth, percentage and essential oil quality of the plant. Thus, the use of Myco-Root biofertilizer instead of chemical fertilizers could be recommended to achieve the sustainable agriculture in the thyme cultivation.

Tomato (Solanum lycopersicum L.) is a common vegetable that is widely cultivated and consumed worldwide in the Solanaceae family (global tomato production is estimated at approximately 182 million tons in 2017). Tomato, because of its elevated nutritional value, is the second most common vegetable commodity in the world after potatoes. As with other crops, the global production of tomatoes is threatened by certain biological stresses (such as pests, plant diseases and weeds) and non-biological stresses (such as salinity, drought, floods, cold and heat stress). Nowadays, the excessive use of chemical fertilizers in tomato production in order to increase yields, has resulted in environmental pollution and dangers on the health of consumers. The reaction of cultivated plants to these challenges is indicated by numerous morphological, physiological, biochemical and molecular changes, leading to a direct and indirect decrease in plant growth and productivity. Salinity as a non-biological stress can cause osmotic or ionic imbalance in plant cells. Salinity stress also limits growth and germination by affecting water and reducing water availability and affects crop production. Endophytes represent an eco-friendly option for the promotion of plant growth and for serving as sustainable resources of novel bioactive natural products. One of the alternative ways to restore normal plant growth under salinity stress may be to use plant growth to stimulate endophytes. Endophytes can play an important role in plant survival under salinity stress by reducing the adverse effects of sodium ion. Therefore, this work provides strong evidence that endophyte halophyte can be beneficial for tomato that help tolerate the plants stress. The main aim of this study was to investigate the role of endophytic bacteria (Exigubacterium aurantiacum), isolated from Salsola imbricate, in improving the growth of Solanum lycopersicum L. (8320) under salinity stress. The salinity tolerance potential of bacterial endophytes was investigated in vitro. The bacterial was cultured in Nutrient Agar with different concentrations of NaCl (1, 2 and 3 M) and its growth dynamics were investigated after 24 and 120 hours. To prepare the bacterial suspension for inoculation with tomato seeds, the bacteria were cultured on NB (Nutrient Broth) medium for 24 hours in an incubator at 28±1 °C at 130 rpm. The OD suspension was adjusted to a concentration of 1×108 ml. Tomato seeds (cultivar 8320) were washed with ethanol (70%) for 30 seconds and then sterilized with 0.5% sodium hypochlorite for 90 minutes and then completely distilled three times with distilled water. They were autoclaved and washed. For better contact of seeds with bacteria, 1% carboxymethylcellulose was used and then the seeds inoculated with bacterial treatments were placed on a shaker for six hours. Seeds inoculated with bacterial endophytes were planted in seedlings and then placed in pots containing autoclaved soil in the greenhouse of the Faculty of Agriculture, Hormoz University. The experiment was arranged in a factorial experiment based on randomized complete block design with three replications. Experimental treatments included five levels of salinity stress (0, 4, 6, 8 and 10 dS/m-1) and bacterial endophyte inoculation (E. aurantiacum). Analysis of variance of traits was performed using SAS software version 9.4 and the means were compared using LSD method with a probability level of p < /em> Analysis of variance showed that among treatments there is significant difference on growth parameters of tomato seedling (p < /em>  0.01), this indicate the positive impact of the bacterial endophyte on the growth parameters of tomato seedling is inoculated with the bacterial than the control plants. In this experiment, stem height, dry weight of stem, leaf and root, percentage of electrolyte leakage, chlorophyll a, chlorophyll b, carotenoid, proline and carbohydrate content were examined. The results of mean comparison showed that salinity stress significantly reduced stem height, stem dry weight, leaves and roots, chlorophyll a, chlorophyll b, carotenoids and increased electrolyte leakage; however, bacterial endophyte reduced the negative effects of salinity stress on tomatoes. Tomato seedling treated with endophytic bacteria showed higher levels of key osmolytes, total soluble carbohydrates and free proline compared to untreated plants under salinity stress. The results also showed that the use of endophytic bacteria increased the growth of tomatoes in saline soil and water, thereby it can be used as an effective tool for growing salinity-sensitive plants such as tomatoes in saline conditions.

Bio-fertilizers of bacteria and fungi that are useful are produced each specific purpose, such as nitrogen fixation, release of phosphate ions, potassium and iron insoluble compounds of them. An experiment to study the effects of Mycorrhizal fungi (Glomusmosseae) and Pseudomonas bacteria (Pseudomonasputida) on yield under drought stress, antioxidant enzymes and biomarkers destruction of the corn plant KSC704 varieties in crop year 2013-2014 was conducted in Varamin. The experiment was a split plot in a randomized complete block design with three replications were studied. The main factor of three irrigation levels (1- Normal irrigation. 2. Cut irrigation in the flowering stage. 3- Cut irrigation at grain filling) and Bio-fertilizer mixing sub Mycorrhizal fungi and Pseudomonas bacteria to the seeds when planting at four levels (1. non-use of Mycorrhiza and Pseudomonas. 2. The application of Mycorrhizal 60 g.ha-1. 3. Application of 100 g.ha-1 Pseudomonas. 4. Application of Mycorrhizal fungi and Pseudomonas) was considered. The results showed that the interactions between stress and the use of Mycorrhizal fungi and Pseudomonas on all traits was significant at the level of one and five percent. The highest grain yield (7965.3 kg.ha-1), biological yield (20468.3 kg.ha-1) and harvest index (39.1 %) were obtained with simultaneous application of Mycorrhizal fungi and Pseudomonas and normal irrigation and The least of these traits of stress during flowering and non-use of Mycorrhizal fungi and Pseudomonas allocated to that with drought stress during grain filling was no significant difference. It should be noted in irrigation and Application of Mycorrhizal fungi and Pseudomonas usually the least amount of catalase, D-OH guanosine and malondialdehyde were obtained. The highest amount of leaf prolinein the amount of 0.336 µg.g-1 in irrigation cut off at flowering stage and the application of Mycorrhiza and Pseudomonas was obtained. Simultaneous use of Mycorrhizal fungi and Pseudomonas conditions of drought stress on the growth, the plant could greatly increase your tolerance through increasing adaptability metabolites

In the past decades, chemical fertilizers are used by farmers have numerous environmental impacts, including various types of water and soil contamination, and cause many problems to human health and other organisms. Sustainable agricultural policy and sustainable agricultural development have prompted experts to make greater use of soil organisms to meet the plant's nutritional needs, and that is why the production of biofertilizers began. Biological imbalances in the field of sustainable agriculture can be attributed to the fungi of myoderma and its species. According to various studies, it seems that this microorganism having high ability to compete for food and space, and the establishment of spores in the environment and in particular soil around the roots of most crops and non-farm and can induce plant resistance not only reduces pathogen agents Vermicompost is a microbiologically rich, nutrient-rich, organic modifier that is produced by the interaction between earthworms and microorganisms during the decomposition of organic matter. This type of organic fertilizer contains the waste of certain species of earthworms as a result of alteration, conversion, and relative organic residues as they pass through the digestive tract of these animals. Research has shown that vermicompost has a positive effect on growth, crop development, and crop yield. The aim of this study was to investigate the effect of different amounts of vermicompost and different concentrations of Trichoderma Bi isolate on different properties of celery.
This study was conducted to investigate the effects of Trichoderma and vermicompost as a biofertilizer promoting growth in research greenhouse at the Ferdowsi University of Mashhad with an average daily temperature of 15-27 °C and relative humidity of 40-70%. The present study was a factorial experiment based on a completely randomized design with three replicates as a pot experiment under no-tillage conditions in the greenhouse. The first factor consisted of four concentrations of Trichoderma harizianum isolate Bi: zero percent (control), 5%, 10%, and 15% volume of 50 liters of water consumed. The second factor also included four vermicompost treatments: zero percent (control), 25 percent, 50 percent, and 75 percent pot volume. Celery seeds were sown in transplant trays. The transplants were ready for transfer to the main litter after 10 weeks. The pots used were of plastic-type with a span diameter of 20 cm and a height of 25 cm. The plant media consisted of a mixture of 20% cocoon and 80% perlite, the roots of which were easily separable. After full harvest of plants at the commercial size, when a complete set of petiole sets was created (40 days after transplanting) morphological traits including the fresh and dry weight of roots and stems, a number of leaves, stem diameter, stem and root lengths were evaluated. The dry and dry weight of plant root was measured using a digital marking scale and with an accuracy of 0.01 g. Dry weight was determined after placing the specimens in the oven at 72 ° C for 48 hours. Also, the stem diameter was measured using a caliper machine with 0.01 mm accuracy. Root and shoot lengths were measured separately in the laboratory by a ruler in cm. Chlorophyll a, b and carotenoids were read at 663 nm, 653 nm and 470 nm for absorption by spectrophotometer, respectively. Data were analyzed using JMP8 software and ANOVA was performed using the LSD test at 5% probability level. Charts were drawn using Excel 2013 software.
The results of this study showed a positive and optimal effect of combined vermicompost and Trichoderma fungi. The highest shoot dry weight (49.23 g), leaf number (46), stem diameter (15 mm) and chlorophyll and carotenoid were observed in the effect of vermicompost and Trichoderma fungi compared to the control treatment. The main stem length (77.20 cm) was affected by the fungus with a 10% concentration at the highest rate compared to other treatments. Also, 50% vermicompost treatment had the highest root length (36.66 cm). The highest chlorophyll a was observed at 75% vermicompost interaction at 15% fungi concentration (10.02 mg / g fresh leaf weight). Application of vermicompost in the culture medium and application of Trichoderma fungus extract resulted in improved growth and yield. As can be seen in the results, the best treatment was 15% and 75% vermicompost, respectively. They can be used to improve plant growth and function. Many researchers believe that mainly isolates of Trichoderma produce biochemical stimuli to stimulate plant growth or reduce the inhibitory effects of certain compounds, biological and chemical toxins. According to available reports, the application of vermicompost with 30% volume in ornamental Lilium increased leaf area, fresh and dry weight of plant and plant height. So, fresh and dry weights of bean stem under vermicompost application significantly increased compared to the control treatment, which is in line with the results of this study. However, the researchers believe that the wet and dry weight gain of the plant body if used with vermicompost is probably due to the high amount of humic acids in this biofertilizer.
Based on the results of this study and other studies on vermicompost as an enriched fertilizer with numerous growth enzymes and hormones, as well as Trichoderma, it can be concluded that the use of bio-fertilizers plays an important role in the production of high-quality products. Also, the effect we find depends on the concentration used, but in the end, even their application at the lowest concentration compared to the control treatment increased growth and morphological characteristics of the plant. Overall, the results of this study showed that, in the case of the studied species, the combination of vermicompost and Trichoderma had a significant advantage over their separate application, and considering the superiority results of most traits at the time of application of these two together. Therefore, a combination of Trichoderma and vermicompost based on the results (concurrent use of 15% fungus extract and 75% vermicompost) is recommended to improve plant growth and yield.

In order to evaluate the effect of zeolite and vermicompost on Marigold (Calendulaofficinalis L.), a field experiment was conducted in 2017 in the research field of Ramin University, Khuzestan, in split plot as a randomized complete block design with three replications. Vermicompost (0, 3, 6, 9 t ha-1) and zeolite (0, 4, 8 and 12 t ha-1) were investigated as the main factor and sub plot, respectively. Results of variance analysis showed that vermicompost and zeolite had a significant effect on flower yield, biological yield, harvest Index, chlorophylls and carotenoids of leaves, petal carotenoids, nitrogen removal, phosphorus removal, and index of the intensity of the color of the petals. Also, interaction effects of vermicompost and zeolite was significant on all top-mentioned characteristics. The highest flower yield (69.53 g m-2) was obtained at 3 t vermicompost per ha and 12 t zeolite per ha. Maximum biological yield (474.84 g m-2) was achieved at 9 t vermicompost per ha. The highest leaf and petal carotenoids were recorded at 9 t vermicompost per ha and maximum chlorophyll a and b belonged to 9 t vermicompost per ha and 4 t zeolite per ha. The highest soil nitrogen harvest was obtained at 8 t zeolite per ha. Maximum soil phosphorus harvest belonged to 9 t vermicompost per ha and 12 t zeolite per ha. In total, knowing that zeolite is more cost-effective rather than vermicompost, increasing the amount of zeolite consumption can enhance flower yield. In the current research, the best flower performance could be obtained at an optimum amount of 3 t vermicmpost per ha and 12 t zeolite per ha.

Semperflorens begonia (Begonia semperflorens) is an ornamental house-plant which for its optimal growth and development needs a proper nutrition. Currently, cyanobacteria are sought after by plant growers as a growth bio-stimulant. Spirulina (Spirulina platensis) is an important cyanobacterium which has a vast range of nutritional organic and inorganic substances. This green-blue algae could be a new option of bio-fertilizers for organic cultivation of plants, which merits further investigations in different plants. In this regard, a research with a completely randomized design and five replications was carried out in the greenhouse of the Department of Horticultural Science at Shiraz University, to increase the quality and yield of semperflorens begonia. Spirulina treatments were studied in two forms of soil fertilization and foliar spray (concentrations of 0, 500, 1000, 2000 and 4000 mg/L). Results showed that soil fertilization has greater effect on morphophysiological traits of begonia than foliar spray. Soil fertilization with 4000 mg/L spirulina was the best treatment and compared to the control, significantly increased the vegetative growth, phosphorus, potassium and chlorophyll content of leaves and visual quality of semperflorens begonia. Also, in the abovementioned treatment, the sugar content of leaves, petals’ anthocyanin content and number of flowers were 3, 2 and 2 times higher than control treatment.

Water scarcity adversely affects many physiological and metabolic processes of the plants. To study the effects of drought stress and fertilization on some physiological characteristics, essential oil percentage and yield of spearmint, a field experiment was conducted at the Research Farm of the College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. The experiment was conducted in a split plot arrangement based on a randomized complete block design with three replications. Treatments included three irrigation treatments (100%, 75% and 50% of plant water requirement) as the main plot and four fertilizer regimes including no fertilizer (control), chemical fertilizer (N), bio-fertilizer (rhizome inoculation with Azotobacter, Pseudomonas and mycorrhiza) and integration of biological fertilizer and 50% chemical fertilizer as the sub-plot. The results showed that all traits measured were significantly affected by the main and interaction effects of deficit irrigation and fertilizer. The content of chlorophyll a, chlorophyll b, carotenoids, total chlorophyll, percentage and yield of essential oil and yield decreased with increasing water stress while the rate of carbohydrate and proline as well as the electrolyte leakage percentage increased. Furthermore, the results showed that under 100% and 75% irrigation systems, application of chemical and integrated fertilizers improved the physiological characteristics, yield and percentage and yield of essential oil. Under 50% irrigation regime, the best performance was related to bio fertilizer.

Bio fertilizers contain a sufficient number of one or more species of beneficial microorganisms and nutrients from the soil are able to convert unused usable form. In order to evaluate the effect of manure into bio-fertilizer and seed inoculation with nitrogen-fixing on agronomic traits of grain corn KSC647 cultivars, factorial experiment in a randomized complete block design with four replications was conducted during 2012-2013 in the College of Agriculture, Islamic Azad University of Varamin. The first factor manure: M1: to use 30 tons of manure per hectare of manure and M2: Non-application of manure, Second factor biofertilizers Azotobacter strains (Azotobacter chroococcum): Az1: seed inoculation with Azotobacter and Az0: non-inoculated seed with Azotobacter and the third factor bio-fertilizers Azospirillum strains (Azosprillum brasilense): As1: seed inoculation with Azospirillum and As0: non-inoculated seed with Azospirillum biofertilizer. The results showed that the highest grain yield, biological yield, grain weight, harvest index, number of seed per ear from animal manure and seed inoculation treatments combined with Azotobacter and Azospirillum was obtained. Specify the use of bio-fertilizers with manure saves 50% of the nitrogen fertilizer. It seems that excess nitrogen from manure improves the vegetative growth at the beginning and in the middle of nitrogen-fixing bacteria able to stabilize the soil nitrogen available to the plants. Synergy of these two factors has led to the growth of nitrogen in the plants, and thus achieved the highest yield.

In order to examine the effects of biological and chemical fertilizers with the use of salicylic acid on the agronomic characteristics of corn as a factorial experiment in randomized complete block design with three replications at the Agricultural Research Station in 2011 was Boroujerd. Factors examined included three levels of phosphorus fertilizer (0, 100 and 150 kg ha), two levels of biological fertilizer (mycorrhizal fungi) (seed inoculation and seed inoculation) and two levels of salicylic acid (0/5 and 1 mM), respectively. The results showed that phosphorus fertilizer and mycorrhizal significant effect on plant height, stem diameter, ear length, ear diameter, grain yield and biological yield, respectively, with the application of 150 kg P ha grain yield of 9006 kg was found that 48% More the treatment of non-usage. In the case of seed inoculation with mycorrhiza on the amount of 8412 kg per hectare, respectively, which was 24% higher than the treatment of non-inoculated seeds. Salicylic acid has a significant effect on plant height, stem diameter, ear length, ear diameter, grain yield and biological yield and harvest index. The application of 1 mM salicylic acid yield of 8316 kg per hectare, respectively, which was 24% higher than the treatment of non-use. Phosphorus and mycorrhiza interaction on grain yield and biological yield was significant. Salicylic acid and phosphorus interaction and effect on yield only three treatments was significant.

In order the effect of Application of bio fertilizer (nitroxin) on quantity and quality of borage under Water deficit stress an experiment in split plot randomized based on complete block design with three replications in 2014 at the Agricultural Research Station of ROUDEHEN Islamic Azad University done. treatments were first factor drought in four levels (control, stress at Vegetative stage, stress at flowering stage, stress at Vegetative flowering stage) and the second factor consisted of four levels of fertilizer treatments (100% chemical fertilizer, 50% chemical fertilizer bio fertilizer (nitroxin), 25% chemical fertilizer bio fertilizer) respectively. Results showed a significant effect of cut irrigation on plant height, essence percentage, mucilage percentage, flower yield, Chlorophyll, but the effect of cut irrigation on essence yield was not significant. Effect of fertilizer treatments on plant height, essence yield, flower yield and Chlorophyll was significant and the percentage of mucilage was not significant. Interactions between cut irrigation and fertilizer treatments on essence yield (1.75 kg\ha), flower yield (481 kg\ha) was significant and 50% chemical fertilizer bio fertilizer was best treatment. The Least flower and essential oil yield in the 100% chemical fertilizer stress at Vegetative flowering stage treatment with an average of 301 and 1.136 kg per hectare was obtained.

Saffron (Crocus sativus L.) quantitative and qualitative yield was assessed in a factorial experiment based on randomized complete block design with three replications. The factors included NPK fertilizers at three levels (0، 50 and 100 percent of the recommended amount of fertilizer) and non-chemical fertilizers in four levels (no fertilizer، vermicompost، bio fertilizers containing Pseudomonas and Bacillus، and integrated application of vermicompost and bio fertilizers). Results showed that the interaction effect of chemical and non-chemical fertilizers was significant on all parameters and fertilization increased the quality and quantity yield of saffron compared to control. Integrated application of 50% of the amounts of chemical fertilizers، vermicompost and bacteria was the superior treatment to increase stigma dry weight، stigma yield، crocin percentage، picrocrocin percentage and safranal percentage by 2. 03، 4. 65، 0. 83، 1. 10 and 1. 46 fold، respectively in comparison with control. Although the effectiveness of integrated application of bacteria with 0% and 100% chemical fertilizers on crocin content، pure vermicompost and integrated application of bacteria with 50% and 100% chemical fertilizers on picrocrocin content and pure bacteria on safranal content were more than those of integrated application of vermicompost، bio and 50% chemical fertilizer، the greatest impacts of recent treatment on flower yield، stigma yield and stigma ingredients led to introduce it as the superior treatment. The effectiveness of this treatment compared to 0%، 50% and 100% of chemical fertilizer application on dry stigma was 50. 95%، 20. 48% and 12. 86%، respectively.

Application of bio fertilizer, especially nitrogen- fixing cooperating bacteria, together with chemo fertilizers tend to be the most important integrated method for Plant nitrogen for Performance management of agricultural ecosystems and increasing production in a stable agricultural system.With Sufficient inputs. In order to in vest gated the effect of biofertilizer Azorhizobium and Nitro Chimo fertilizer on quality and quantity Properties of different Brassica Cultivar, an experiment using factorial completely randomized block design in triplicate was performed in Research Field of Islamic Azad University, Varamin. The treatments were at four levels including Nitro fertilizer, biofertilizer Azorhizobium, 50% Nitro fertilizer Azorhizobium, and 25% Nitro fertilizer Azorhizobium as well as at three levels of rape cultivars including Hyola402, Hyola401, RGS. The results of this study showed that using N- fixing cooperating bacteria (Azorhizobium) improved morponents performance. Also it was demonstrated that in the presence of these bacteria, the amount of applied nitro chemo fertilizer is reduced to one – fourth of the recommended Value on the basis of Soil test. In this study, the best performance of 25% nitrogen fertilizer, manure and biofertilizers on Hyola401 in Azorhizobium respectively.