جستجوی مقالات مرتبط با کلیدواژه « زخم پوستی » در نشریات گروه « پزشکی »

Mesenchymal stem cells (MSCs) have been identified as an attractive source for cell therapy of damaged tissues. The multipotential differentiation capability, easy accessibility, low immunogenicity and significant role in wound healing physiology have led to the widespread use of these cells for wound healing. In this review study, the role of mesenchymal stem cells in repair of skin wounds is discussed .

This review was written based on findings from a search of the PubMed and Google Scholar databases between 1990 and 2020.

Mesenchymal stem cells have important roles at all stages of wound healing. These cells migrate to the site of skin damage and not only differentiate into the skin cells but also through paracrine signalling inhibit the immune and inflammatory responses, induce proliferation and differentiation of resident progenitor cells and result in angiogenesis, epithelialization and granule tissue formation. In the regeneration and maturation phase, the MSCs reduce scar formation and wound contraction and increase collagen expression and wound tensile strength. These cells alter their function based on the biomolecular properties of the lesion site, and create a wound-healing rather than fibrotic microenvironment .

Todays, significant advances have been made in the delivery of MSCs into healing wounds. These cells are delivered via intravenous or intradermal injection, or delivered with scaffolds, as a fibrin polymer spray or with hydrogels. In addition, the extracellular vesicles and conditioned medium of MSCs alone are effective. Future studies could lead to more effective strategies for the use of mesenchymal stem cells in wound healing . Received

Wound healing is an important concern that humankind is facing and attempting to find compounds that have the least side effects on wound healing is important. Since violet flower extract has anti-inflammatory and disinfection effects, in this study the impact of this plant on wound healing was examined.

32 rats were provided from the laboratory animal breeding center in Shahrekord, and after anesthetizing them, a full-thickness skin wound was created by a 2 by 2 Cm stencil in the posterior cervical region. The control group received no treatment, one group received Eucerin, another group received Silver Sulfadiazine 1% and the experimental group received ointment of violet flower extract 20% twice a day. Throughout the study, the length and width of the wounds were measured in each group every three days. On the 3rd, 7th, 14th and 21st days after creating the wound, the rats were anesthetized and the healed tissues were examined for histopathologic studies.

The results showed a significant reduction in wound area in treated animals, especially violets, compared with the control group (P<0.05). Histopathologically, the thickness of the epithelium and the amount of collagen showed a significant increase in the experimental group of violets.

This can probably be attributed to the anti-inflammatory effects of the effective components of violet flower. also it’s likely that these components stimulate collagen production and quicker contraction of wounds. Therefore, the compounds of this plant can be used to treat wounds.

post-operative surgical wound healing is highly important, so, it could be of great benefit to find suitable alternatives for chemical drugs through herbal remedies in Persian Medicine. The purpose of this study was to determine the effect of Arnebia euchroma ointment on surgical wound healing process.

In this experimental study, 24 Wistar rats were randomly divided into four groups (n=6 per group); controls (no intervention), positive controls (silver sulfadiazine cream 1%), intervention group I (Arnebia euchroma ointment), and intervention group II (mixed Arnebia euchroma and silver sulfadiazin cream1%). In all groups, an ulcer was developed in the back of the neck, then the groups were treated for 21 days. Wound healing was evaluated at days 4, 7, 14, and 21. Data were analyzed in SPSS V22 applying one-way ANOVA and Games- Howell post hoc test for normal distribution and Kruskal-Wallis test for non-normal distribution.

Surgical wound healing was found be significantly different between intervention group I and control group at days 4 (P= 0.00) and 7 (P= 0.02). Percentage of wound healing at day 14, in control group, the positive controls, and intervention groups I and II were 81.16±4.4, 84±19.27, 94.28±4 and 82.20±19, respectively. The rate was considerably higher in intervention group I (P= 0.07).

Topical use of Arnebia euchroma root ointment can facilitate surgical wound healing.

Diabetes mellitus is highly prevalent in the world and poor wound healing in diabetic patients is a serious public health issue nowadays. In this study, we evaluated the healing effects of olive leaf extract ointment on cutaneous wound in diabetic rats. Thirty six male rats were di vided into three groups: a control group and two experimental groups to receive either olive leaf ointment or derma heal ointment. Diabetes was induced by a single streptozotocin injection (65mg/kg). Three days later, fasting blood glucose was measured by a glucometer. Rats with blood glucose levels above 250 mg/dl were considered as diabetic. Under anesthesia, a circular full-thickness incision (10 mm diameter) was made in shaved dorsal region. Animals in control group received only distilled water, while treatment groups were treated with ointments (once/daily) until the end of the experiment. To investigate the effects of olive leaf ointment on diabetic wounds, macroscopic and microscopic studies were performed at days 4, 7, 10, 14, and 21. Macroscopic and microscopic evaluations showed significantly higher rate of wound healing in experimental groups compared to the control group in all days after wound creation (P<0.05). But, wound contraction was significantly higher in the group receiving derma heal ointment compared with that in the group treated with olive leaf ointment (P<0.05). There were no significant differences between the two experimental groups in microscopic indices (P>0.05). In this study, wound healing was significantly promoted when diabetic wounds were treated with olive leaf ointment.

Exfoliative toxin is Staphylococcus aureus various factors that can caused colonization and virulance. The aim of this study was to investigate the presence of several antibiotic resistant genes on exfoliative B-encoding plasmids in S. aureus infected skin specimens. 100 wound samples collected from Tehran Hospitals. Bacteria were identified by standard biochemical and microbiological tests. Antibiogram test was performed for S. aureus isolates. DNA was extracted of resistance S. aureus. Presence of antibiotic resistant etb, aac, and msrA genes were identified by Muliplex-PCR. From 100 samples, 60 samples were S. aureus. Resistance to erythromycin, gentamicin, tobramycin, ciprofloxacin and linzolide was 3 (5%), 1 (2%), 1 (2%), 1 (2%) and 0 (0%) respectively. Frequency of msrA, aac and etb genes were 29 (48.3%), 45 (75%) and 37 (61.7%) respectively. Frequency for simultaneously presence of the etb / aac, etb / msrA, msrA / aac and msrA / aac / etb gene were 28 (46.6%), 22 (36.6%), 22 (36.6%) and 18 (30%) strains. . In this study, etb gene was indicated in most strains. As also, investigation of virulence genes in S. aureus skin infection samples showed frequency of resistance pathogen genes were increased.

Skin wounds are a common event in diabetes. Plants and their extracts known as phytomedicine have immense potential for the management and treatment of skin wounds. The aim of this study is investigation of effect of Nigella sativa seeds extract (NSE) on skin wound healing in male diabetic rats.
In this experimental study, 49 Wistar male rats (weighing 220-250 g) were randomly divided into seven groups of equal numbers, including: The control group that were healthy and without medication (A), healthy rats used of phenytoin 1% (B), receiver of eucerine or sham(C), diabetic rats without treatment (D), diabetic rats treated with phenytoin cream 1%(E) and diabetic groups received NSE ointment 20%(F) and 40%(G). Diabete was induced via stereptozotocin 60 mg/kg. After anesthetization by Ketamine 10% (50mg/kg) Xylazine 2% (10mg/kg), skin wound (1cm×2cm ) were done on the back of all animals and medical prescription and measuring wounds performed until perfect recovery. For histopathological studies, some biopsies were taken on different days. The data were analyzed using one-way ANOVA and Turkey’s tests.
The average healing time for D and E groups were took place in 27th and 24th days after beginning of skin wound respectively. Healing time lasted 23, 24 and 21 days respectively in control and sham and B groups. The shortest time to heal the wound were in diabetic groups received NSE ointment 20 %(18 days) and NSE ointment 40%(15 days).These two groups also had the smallest mean surface of wound. The mean of surface wound in groups that treated with nigella sativa extract showed significant difference with control group (P The results of this study showed that the best healing effect was from nigella sativa extract40% ointment. With regard to anti-inflammation and anti-microbial effects of nigella sativa, it maybe this plant has effects on wound healing process.The antioxidant compounds which present in NSE are able to accelerate the healing process compared to conventional drugs.

Wound healing is a complex patho-physiological process which includes cellular and biochemical reactions such as inflammation, vessel formation and collagen deposition. In the present study, the anti-inflammatory and treatment effects of hydro-alcohol extracts of Plantago lanceolata, Taraxacum officinale And Quercus robur were compared with the effect of zinc oxide after wounding rats.
Forty male rats underwent anesthesia, then a circular wound with a range of 280 mm2 was created on the back of the rats. The treatment process included five groups as false control, positive control and treatment with extracts of Plantago lanceolata, Taraxacum officinale And Quercus robur. The drugs administration and measurement of the wounds were done by digital image analysis in 21 alternating days. The analysis of the data was carried out using one-way ANOVA.
The percentage of wound healing increased significantly in groups with extracts of Plantago lanceolata and zinc oxide compared to the control groups in fifth, ninth and thirteenth days. The percentage of wound healing significantly P The fastest healing of wounds was dedicated to the hydro-alcohol extract of Plantago lanceolata Hydro-alcohol extract of Plantago lanceolata which had more anti-inflammatory effects compared to zinc oxid by reducing the inflation, and decreasing the duration of complete wound healing.

Healing cutaneous wounds is regarded as one of the most important issues in the medicine. Different chemical agents have been used in regard with promoting wound healing, most of which unfortunately present some side effects and defects. Since natural combinations have proposed less disadvantages, this study aimed to investigate the effect of Withania Somnifera root extract on cutaneous wound healing in the male rats. This study was performed on 36 rats weighing 180-220g that were divided into 6 groups (n=6). First, wounds (2x2) were made on the dorsal skin of the animals. The first group was left without treatment (control group), the second was treated with Eucerin(negative control), the third group received 1% of phenytoin cream and in the other groups, different concentrations of hydroalcoholic extract of Withania Somnifera (20%, 40%, 60% w/w) combined with Eucerin base were administrated once per day. The area of wounds was measured by Autocad software every day, from the 2nd day to 14th day. The study data were analyzed via SPSS software (ver.16) at the significant level of P<0.05.The reduction of incisional wound area in the all groups treated with root extract of Withania Somnifera ointment was significantly higher on the 8th day. Moreover, dose of 60% and 90% revealed better effects (p<0.001). The findings of the present study demonstrated that Withania Somnifera root, due to its significant reduction in the healing time as well as wound area, can be used as an effective material in regard with the cutaneous wound healing.

Probiotics are defined as different microorganisms that may have positive effects on preventing or treatment of special pathologic conditions. The actions of lactic acid bacteria are species and strain specific, and depend on sufficient numbers of bacteria being available in the intestines. The difficulty in identifying and classifying strains has complicated research, since benefits may only pertain to particular strains. Since there is no research regarding Iran’s probiotics up to now thus, the present study was designed to investigate the effect of Lactobacillus brevis on cutaneous wound healing. In this experimental study, 22 strains of Lactobacillus isolated from dairy-traditional products of exopolysaccharide production are investigated by phenol-sulfuric acid method. Lactobacillus brevis, which had high exopolysaccharide (EPS) production, was selected. A full-thickness square wound (1.5 × 1.5 cm) was made on the back of each rat (45 rats in 3 groups). Two groups, control and experimental were treated by eucerin and eucerin contained 1×1o 11 CFU/ml Lactobacillus brevis, but the negative-control group did not receive anything. Rats were killed on days 1, 3 and 14 and wound samples were collected for histological and statistical studies. The data were expressed as mean±Standard deviation. Statistical analysis was performed with ANOVA. The percent of wound healing (91.76%) and inflammation in experimental group on day 14 as compared to control (74.33%) and negative groups (73.51%) was significant (p<0.001). The numbers of neutrophils in experimental group were reduced in later phase of wound healing as compared to control and negative groups. The current study showed a significant reduction in inflammation and a significant acceleration in wound healing on the rats treated by Lactobacillus brevis as compared to control and negative control groups. Further studies are required for detail mechanism of Lactobacillus brevis during wound healing.

Today, there is growing interest in using traditional medicine for healing cutaneous wounds. Probiotics are defined as different microorganisms that may have positive effects on prevention or treatment of special pathologic conditions. The present study was designed to investigate the effect of Lactobacillus brevis on cutaneous wound healing. In this experimental study, through phenol-sulfuric acid method, 22 strains of lactobacillus isolated from dairy-traditional products were investigated in terms of exopolysaccharide production. Lactobacillus brevis, which had high exopolysaccharide (EPS) production, was selected. A wound was created on the back of male Wistar rats in control, negative control, and experimental groups. Control and experimental groups underwent regional treatment by eucerin and eucerin contained Lactobacillus brevis, respectively, but the negative-control group did not receive any treatment. On days 1, 7 and 21, the rats were killed and their cutaneous wound samples were studied. Data analysis was done through SPSS version 11.5. The percentage of wound healing (99.53%) and inflammation in the experimental group on day 21 compared to control (90.55%) and negative groups (91.14%) was significantly higher (P<0.001). The number of neutrophils in the experimental group decreased in later phases of wound healing compared to the control and negative control groups. The present study showed that Lactobacillus brevis significantly decreases inflammation and accelerates wound healing in treated rats. The findings of this study can be applied clinically in near future.

Background and Ârtemisia aucheri (Âra) is a plant with evident anti-inflammatory and anti-microbial properties. Ïn the present study، we investigate the effect of hydro-alcoholic extract of Âra on skin wound healing among male rats. 30 male Wistar rats were randomly divided into 3 equal groups: control group receiving saline as wound treatment and two study groups receiving 10 and 100 mg/ml of Âra extract. Saline and Âra were locally applied starting one day after wound introduction on a daily basis. Wound areas were measured on days 1،5،9،13،17 and 21. Data are presented in mean ± SËM and analized using repeated measure ÂNÔVÂ test. The P<0. 05 was considered as a significant difference. The area of wound was significantly different at days fifth، ninth (P<0. 001)، thirteenth (P<0. 01) and seventeenth (P<0. 05) between control and both Âra extract receiving groups. The amount of wound healing in percent was also significantly different between control and both Âra extract receiving groups at days fifth، ninth (P<0. 001)، thirteenth and seventeenth (P<0. 05). The duration of complete wound healing was also significantly different (P<0. 001) between control and treated groups. Çonclusion: Hydro-alcoholic extract of Ârtemisia aucheri accelerates skin wound healing process and decreases the duration of complete wound healing.

Background and Management of cutaneous wounds is an important subject in medicine. Different chemical agents have been used for wound healing but each of them has side effects. In this study, we examined the effects of an experimental ointment derived from a natural product on wound healing. Twenty-four white rabbits divided into three groups as fallows: control, Eucerin and Gazangebine. The ointment was made by Gazangebine extract (9%) and Eucerin in the laboratory. A full thickness wound (2cm ´2cm) was made on the left flank of the test animals. The ointment was applied to the wound area twice daily without any covering as an open wound. Wound area was measured every 3 days until day 18. In this day, rabbits were biopsied to look for histopathological changes. Gazangebine ointment had no beneficial effects on wound contraction. However, it decreased inflammation and neutrophil number (P<0.05, p=0.001) and increased eosinophil number and dermal tissue healing (p<0.05, p=0.000). The results suggest that Gazangebine ointment has positive effects on wound healing.

Background and About 200,000 of Iranian population are infected with Hepatitis C Virus. On the basis of Iranian consensus on management of chronic hepatitis C (CHC) infection in 2004, combination therapy with conventional α-Interferon (IFN) plus Ribavirin is the first therapeutic choice in CHC patients. Data are limited on treatment response rate in most areas of the country. To assess the treatment results, we performed this study in a referral center in province of Fars, in Iran, to compare with the response rates to therapy in Western patients. A total of 118 patients with CHC (males 88%) with mean age of 37.47 (SD 11.4) years, mean Histologic Activity Index of 8.21 (SD 4.5), and mean pretreatment Alt level of 81.26 (SD 72.86) were enrolled in the study. IFN 3 million unit/TIW plus Ribavirin 1-1.2 gr/d were administrated for 6-12 months (depending on genotype), and the patients were followed up for 6 months period to assess sustained virologic response (SVR). The most common route of aquisition of the virus was intravenous drug using seen in 39.8% of patients. 40.7% were infected by genotype 3a, 22 % 1a and 9.3% by 1b. Seventy-three patients completed the course of therapy. End of treatment response (ETR) was achieved in 54 (74%) patients. SVR rate was 48% with a relapse of 13.7%. Hepatic decompensation was seen in 2 (2.7%) patients in follow-up period. With respect to the duration of therapy, genotype of viruses was not significantly different in both responders and non-responders (0.05). Despite the different genotyping pattern, ETR and SVR rates were comparable in Iranian CHC patients treated by combination therapy of conventional IFN plus ribavirin in comparison with Western patients. SVR was greater in cases with pretreatment lower HAI.

It has been shown that the elec trical stimulation has improved th e skin blood circ ulat io n and wo und he ali ng in anima ls. T he prese nt study was co nducte d to de te rm in e a standa rd model of minimum curr e n t a nd period o f e le ctrical stimula tio n th at will in flue nce wound he aling. For this propose 35 mm incisions (35 mm leng th) wer e made at the bac k o f Dutch rabbi ts. Due to the pr o pose previo us studies th e five gro ups animals were stimulated by the electrical impulses (80 Hz and 500 ms dura tion) as followed: Gro up one; four times daily, 4 days, 10 min. Group two: four times da ily, 4 days, 30 min. Group th ree: one t ime daily, 4 days, 120 min. Group four: two times daily, 7 days, 30 min. Group five: four times daily, 7 days, 30 min. Group six: Received no electrical stimulation and considered as a control group. the wound's length, area and period of healing were recor ded as well as the ten sile strength. The results indicated that the duration of wou nds-healing in all experimental gro ups was significantly less than contro l group (P < 0.05). Regard to effects of electrical currents on the skin blood now and the least of e lect rica l st imula tio n in gro up one and sign ifica n tly reduced healing per iod in this group in compariso n with control gro up and other indices as well, we suppose that incre asing freq ue ncy of electrical stimulation while shortening time duration, improve the skin blood circulation and wound healing period while decreasing the side effects.