جستجوی مقالات مرتبط با کلیدواژه "لوسمی میلوئیدی حاد" در نشریات گروه "پزشکی"

Leukemia and its treatment is a long process and requires adherence to self-care behaviors. Orem's self-care deficit nursing model provides a framework for the nursing process in this direction, therefore this study aims to apply The nursing process based on Orem's self-care deficit model was performed on a patient with acute myeloid leukemia.

In this case report, the nursing process of a client suffering from acute myeloid leukemia, referred to Beheshti Hospital in Hamadan city in 1401, based on the Orem's self-care deficit model, was presented. Data collection was done by using Orem evaluation form and by examination, interview, and observation methods. Based on the general self-care needs, development, and deviation from the patient's health, nursing diagnoses were determined, then planning and setting goals were done and the type of nursing system used was determined. Finally, nursing actions were performed based on promoting self-care, and the achievement of the patient's goals and progress was evaluated as a factor of self-care.

Based on the recognition of the patient's self-care needs, ten nursing diagnoses were determined, and finally, the implementation of the nursing process based on the Orem model led to an increase in the quality of nursing services along with an increase in the patient's self-care performance regarding the problems caused by his illness, so that improving the patient's self-care ability in relation to pain control, reducing Risk of infection, control of bleeding and improvement of sleep, reduction of fear and dependence, an increase of motivation and self-confidence and sense of independence was observed in the patient.

Orem's self-care deficit model greatly helped to identify and comprehensively understand the patient's needs and facilitated the achievement of care goals by involving the patient in self-care.

Acute promyelocytic leukemia is a malignancy of myeloid cells that are associated with resistance to apoptosis and differentiation arrest in promyelocytes in the bone marrow. The aim of this study was to investigate the effect of Origanum vulgare aqueous extract on apoptosis induction in an acute promyelocytic leukemia cell line (HL-60).

 The viability of HL-60 cells under treatment with various doses of Origanum vulgare extract was assessed by using a cell cytotoxic assay. Then, IC50 values​​ were determined after 24, 48, and 72 hours. The expression of Caspase8, Caspase9, Bax, Bcl2, and Nrf2 genes were measured by real-time PCR assay and different stages of apoptosis and necrosis in HL-60 cells were investigated using acridine orange/ethidium bromide double staining. Finally, data were introduced into SPSS software and analyzed by one-way ANOVA.

The results showed that Origanum vulgare extract decreased HL-60 cell survival and Caspase9, Caspase8, Bax, Bcl2, and Nrf2 gene expressions were changed after 72 hours of treatment with 1/5 of IC50 of Origanum vulgare extract. Also, morphological changes in the nucleus indicated apoptosis induction in HL-60 cells.

It seems that the aqueous extract of Origanum vulgare leaves can induce apoptosis in an acute promyelocytic leukemia cell line (HL-60) under experimental conditions.

In acute myeloid leukemia, a large number of immature cells develop, which can related to some single nucleotide polymorphisms presence in positions of  genes  that encodes enzymes involved in cell activation and evolution signaling pathways. In this study, the association of rs104893674 (A / C) polymorphism with the risk of Acute Myeloid Leukemia (AML) in samples obtained from Fars and Isfahan Province hospitals was investigated. 

Methods & Materials:

 In the present case-control study conducted at Islamic Azad University of Kazerun in 2019, 94 AML patients and 99 age and sex-matched healthy individuals were enrolled. The rs104893674 (A / C)   polymorphism was determined by Tetra Primer ARMS PCR method. Data were analyzed by SPSS (version23) software using Chi-square statistical test.

Ethical Considerations: 

This study with research ethics code IR.IAU.KAU.REC.1398.051 has been approved by Research Ethics Committee of Islamic Azad University of Kazerun.

 The results of this study showed a significant, allele and genotype-specific Association between rs104893674 (A / C) polymorphism with risk of AML. Thus, there are more likely to develop AML in AC genotype, individuals with A allele at this polymorphic site (P=0.000). 

 The association of acute myeloid leukemia with the genetic polymorphism of the ZAP-70 protein can be considered as an option for prognosis of this complication in susceptible individuals.

Acute myeloid leukemia is a heterozygous hematologic malignancy that is manifested by the     accumulation of hematopoietics stem cells in peripheral blood and bone marrow. Anticancer effects and  cryotoxic activity of curcumin have been proven frequently in many cancers. Sorafenib is known as an inhibitor of angiogenesis which prevent cells’ survival. In the present study, the effects of curcumin and sorafenib and their combination were evaluated on AML cells. 

In this experimental study, U937 and KG-1 cells were treated with different concentration of curcumin and sorafenib and their combination.  MTT assay was applied to assess the viability of cells.  Percentage of apoptotic cells was evaluated by annexin PI staining. Also Real Time PCR was performed to investigte the level of  AKT mRNA expression. 

Our data showed that the percentage of apoptotic cells significantly increased in response to treatment with  curcumin (40µM in KG-1 and U937 cell lines), sorafenib (5 µM and 7 µM in U937 and KG-1, respectively) and their combination. Moreover, the mRNA level of AKT gene was downregulated in KG-1 and U937 cells. 

Conclusions:  

Our results suggest that combination of curcumin and sorafenib could lead to promote apoptosis. Furthurermore, downregulation of AKT gene shows that these agents can be considered as effective agents on AML cells.