جستجوی مقالات مرتبط با کلیدواژه « pgc-1α » در نشریات گروه « پزشکی »

Exercise induces various adaptations, including increased antioxidant capacity, enhanced mitochondrial size, and improved insulin signaling. The present study aimed to investigate the effects of aerobic exercise along with aqueous garlic extract consumption on markers of cardiac mitochondrial biogenesis in diabetic rats.

In this experimental study, 40 male Wistar rats (5 weeks old, weighing 162.1 ± 15.2 grams) were obtained from the Pasteur Institute. Eight rats were assigned to the healthy control group (CN). The remaining 32 rats were rendered diabetic through a single intraperitoneal injection of streptozotocin (60 mg/kg) and were randomly divided into four groups: diabetes (DM), diabetes + exercise (TDM), diabetes + garlic (GDM), and diabetes + exercise + garlic (TGDM). The exercise groups underwent an aerobic exercise program on a treadmill for 8 weeks. Additionally, each rat received 1 milliliter of garlic extract (0.4 g per 100 g of body weight) via gavage daily. Changes in the expression of PGC-1α and Nrf2 genes were measured in heart tissue using real-time PCR.

Induction of diabetes resulted in a significant decrease in PGC-1α (P=0.0001) and Nrf2 (P=0.0001) gene expression compared to the control group. In the TDM group, PGC-1α and Nrf2 expressions significantly increased (P=0.038 and P=0.031, respectively), as did those in the GDM group (P=0.036 and P=0.025, respectively) compared to the DM group. However, the combined intervention of exercise and garlic supplementation did not yield significant improvements compared to each intervention alone regarding PGC-1α and Nrf2 expression (P< 0.05).

Both garlic supplementation and exercise may inhibit streptozotocin-induced cardiac damage by upregulating the PGC-1α and Nrf2 pathways. Further studies are warranted to explore this area more comprehensively.

Atrophy of the skeletal system caused by spinal cord injury (SCI) can cause secondary systemic metabolic dysfunction, such as glucose intolerance, type 2 diabetes, and insulin resistance. On the other hand, Sirtun1 (SIRT1) and Peroxisome Proliferator-Activated Receptor-Gamma Coactivator-1alpha (PGC-1α) are known factors and related proteins involved in skeletal muscle atrophy. Therefore, the present study aimed to investigate the effect of aerobic training with resveratrol consumption on SIRT1 and PGC-1α levels in the gastrocnemius muscles of rats after spinal cord injury.

36 male Wistar rats aged eight weeks were randomly placed in 4 groups including control, resveratrol, training, and resveratrol+training (each group n=9). After anesthesia, an incision was made in the midline of the back and over the vertebral ridges. The muscles and lamina of the T9 vertebra were removed without damaging the dura mater, Spinal cord injury was caused by dropping a ten-gram weight from a height of 25 mm on the spinal cord in the T10 segment. After confirmation of SCI, a Resveratrol supplement with a dose of 10 mg/kg was injected intraperitoneally and daily (every morning for 4 weeks), and the rats of other groups were injected with the same amount of saline. The aerobic training was carried out with the help of the weight support system for 4 weeks, 5 sessions per week, each session was 58 minutes and the intensity was 20 m/min. 48 hours after the last training session, the gastrocnemius muscle of right leg of all rats was removed. The SIRT1 and PGC-1α levels in the gastrocnemius muscle were measured by the ELISA method. To analyze the data, one-way analysis of variance and LSD post hoc tests were used at the significance level of P<0.05.

SIRT1 levels in the gastrocnemius muscle of resveratrol and resveratrol+training groups were significantly higher than the control group (P-value respectively 0.023 and 0.007), but despite the increase in the level of this index in the training group compared to the control group, no significant difference was observed (P=0.399). SIRT1 levels in gastrocnemius muscle of resveratrol+training groups were significantly higher than training group (P=0.038). PGC-1α levels in gastrocnemius muscle of training and resveratrol+ training groups were significantly higher than control group (P-value respectively 0.024 and 0.007), but despite the increase in the level of this index in the resveratrol group compared to the control group, no significant difference was observed (P=0.449). PGC-1α levels in gastrocnemius muscle of resveratrol+training groups were significantly higher than reveratrol group (P=0.023).

These results show the positive effect of resveratrol consumption, on SIRT1 level, as well as aerobic exercise on PGC-1α level of the biceps muscle in rats with SCI. It has been pointed out that the increase in SIRT1 expression and the subsequent increase in PGC-1α leads to an increase in the number of myonuclei, which improves the recovery process after injury and plays an active role in muscle hypertrophy by upregulating and reducing catabolic processes. herefore, it appears that a combination of aerobic training and resveratrol supplementation can mitigate muscle atrophy caused by spinal cord injury in muscles below the level of injury by influencing the levels of muscle SIRT1 and PGC-1α

Sports activity increases the expression of PGC-1α, the regulatory factor of mitochondrial biogenesis. This study aimed to investigate the effect of eight weeks of Moderate-intensity Continuous Training (MICT) and High-intensity Intermittent Training (HIIT), along with sodium citrate supplementation, on PGC-1α expression in male Wistar rats.

Forty rats were placed in the following five groups: Control, MICT, MICT+sodium citrate, HIIT, and HIIT+sodium citrate. The continuous training group ran for eight weeks and five sessions per week with an average intensity of 70% of the maximum running speed for 15-30 minutes. The interval training group ran for 12 minutes with an intensity range of 50% and 90%. In addition to exercise, the exercise+supplement groups received 15 mmol/l sodium citrate supplement daily. The expression of PGC-1α in the soleus muscle was measured by the western blot method. Data analysis was done using one-way analysis of variance and Tukey's post hoc test.

The PGC-1α protein expression increased in both the training groups and the training+supplement groups compared to the control group (P=0.001). Furthermore, adding sodium citrate could significantly increase the PGC-1α expression in the MICTgroup+supplement compared to the MICT group (P=0.005). However, the HIIT had a higher effect on PGC-1α expression than the MICT group.

Despite the effectiveness of both training methods, sodium citrate supplementation before HIIT could have more synergistic effects in increasing the expression of PGC-1α.

It has been shown that dysfunction in mitochondrial biogenesis of the skeletal muscles induced by elderly can cause various diseases. The aim of this study was to assess effects of eight weeks of high-intensity interval training (HIIT) with hawthorn (Haw) supplementation on PGC-1α and TFAM in extensor digitorum longus (EDL) muscles of elderly male rats.

In this experimental study, 36 elderly rats were randomly assigned into the following groups of control (CON), HIIT and Haw as well a combination of the two latter ones (COM, HIIT and Haw). The HIIT program consisted of 6 to 9 1-min bouts of running on a treadmill at a speed of 16 to 25 m/min with 1-m rests between the intervals. During the intervention, rats in Haw and COM groups were fed (roughly 100 mg/kg/body weight) via gavage. The PGC-1α and TFAM proteins in EDL muscles were assessed using western blot method.

The PGC-1α protein in COM group was significantly higher than that in CON (p = 0.0001), HIIT (p = 0.008) and Haw (p = 0.0001) groups and was higher in HIIT (p = 0.0001) and Haw (p = 0.0001) groups than CON group. The TFAM protein increased significantly in COM group, compared to CON (p = 0.0001), HIIT (p = 0.014) and Haw (p = 0.0001) groups. Furthermore, this protein was higher in HIIT group, compared to CON (p = 0.0001) and Haw (p = 0.0001) groups. 

These findings have indicated that combination of HIIT and Haw can cause greater increases in mitochondrial biogenesis proteins.

HIIT exercises play a role in increasing the expression of peroxisome proliferator-activated receptor gamma co-activator alpha (PGC-1α) and nuclear factor erythroid-related factor 2 (Nrf2). The aim of the present study was to determine the effect of high-intensity interval training with sodium citrate on the expression of PGC-1α and Nrf2 in the soleus muscle of rats.

In this interventional study, 24 three-month-old white male rats were divided into three groups of control, HIIT exercises, and HIIT exercises + citrate supplement, and were matched according to their weight. Rats in two groups of HIIT exercises and HIIT exercises + citrate supplement performed interval training for 8 weeks and 5 days each week with 50% amplitude and 90% maximum intensity, respectively, in the form of running on a treadmill. In addition to exercising, the rats in the HIIT + citrate supplement group received 15 mmol/L of sodium citrate supplement as a solution in water. The expression of PGC-1α and Nrf2 was measured in soleus muscle by western blotting method. Data analysis was done using one-way analysis of variance and Tukey's post hoc test in SPSS software at a significance level of 0.05. Also, Cohen's D effect size was used to compare groups.

The results showed that there was a significant difference in the expression of PGC-1α and Nrf2 protein in two groups of HIIT exercises and HIIT exercises + citrate supplement compared to the control group (p=0.001). Also, the addition of sodium citrate might have a significant effect on HIIT exercises + citrate supplement group compared to the HIIT exercises (p=0.001).

HIIT training increases the expression of PGC-1α and Nrf2. Also, sodium citrate supplementation before HIIT exercises can have a double effect in increasing the expression of PGC-1α and Nrf2.

Hyperglycemia causes disorders of cardiovascular system, kidneys, retina, lens of the eyes, skin, and central and peripheral nervous system. Since two indicators PGC-1α and Atf2 are involved in mitochondrial biogenesis, therefore, this study was designed and implemented to evaluate the effect of 6 weeks’ endurance training with quercetin supplementation on the expression of mitochondrial PGC-1α and Atf2 genes in the hippocampus of diabetic male rats.

The present study was an experimental study with a post-test design with a control group that was performed by laboratory method. In the present study, 35 Wistar rats were divided into 5 groups (7 in each) including: healthy control, quercetin supplement, control diabetic, exercise diabetic, and diabetic quercetin supplement. 15 mg of quercetin per kg of body weight were injected to two experimental groups (diabetic exercise supplement and supplement) each day after diabetes induction. The protocol used in the present study was 6 weeks’ moderate intensity endurance training (55-50% of maximum oxygen consumption). Hippocampal tissue was taken surgically 24 hours after the last training session to evaluate the expression of PGC-1α and Atf2 genes. One-way analysis of variance with Tukey post-hoc test (with a significance level of P <0.05) was used to examine the data.

Results showed that the expression of PGC-1α and Atf2 genes after 6 weeks of intervention, increased significantly in the diabetic supplement training group compared to the diabetic control, training diabetes, and quercetin supplementation groups (P<0.05). The diabetic exercise group had a significant increase compared to the diabetes control group for both of the genes (P <0.05).

It seems that 6 weeks’ endurance training alone and in combination with quercetin supplementation has a positive effect on mitochondrial PGC-1α and Atf2 gene expression, and increases them in the diabetic patients.

High intensity interval training can promote PGC-1α and TFAM key regulator of mitochondrial biogenesis. Then the purpose of this investigation is to examine the effect of high intensity interval training whit supplementation of sodium citrate on PGC-1α and TFAM expression in soleus muscle of Wistar rats.

24 Wistar rats were randomly divided into 3 groups of control, training and training with sodium citrate supplementation. Both training and training with sodium citrate supplementation groups performed high intensity interval training for 8 weeks, five days a week, with the intensity of 50% to 90% maximum running speed on rodent treadmill. The training with supplement group were treated with a dose (15 mmol/L) of sodium citrate by drinking water per day for 8 weeks. Statistical evaluations of the experiments were investigated by applying One-Way ANOVA and Tukey’s post hoc analysis with a statistical level of P < 0.05. Cohen’s d also was calculated to compare groups.

Findings indicated significant differences in PGC-1α and TFAM protein level in both training and training with supplement groups compared to control group (P = 0.001). Results about sodium citrate supplementation also showed significant differences in training with supplementation group compared to training group (P = 0.005).

It seems sodium citrate supplementation can induce expression of PGC-1α and TFAM and increases level of PGC-1α and TFAM proteins.

Diabetic myopathy is one of the major problems in people with type2 diabetes that knowing its mechanisms can be helpful in controlling and preventing this disease. PAX7 and PGC-1α are two proteins involved in the renewal and metabolism of carbohydrates in skeletal muscle. The aim of this study was to evaluate the effect of 8weeks of resistance training under hypoxia on the content of PAX7 and PGC-1α proteins in the horseshoe muscle of type 2 diabetic rats.

In this study, 40 male Wistar rats, 10weeks after induction of type2 diabetes, were divided into five groups: healthy control (HC), diabetic control (DC), resistance training (RT), and resistance training in hypoxia (RT-HPX) and hypoxia group (HPX) were divided. Resistance exercises were performed for 8 weeks, 5 sessions per week, in the groups of resistance training and resistance training in hypoxia. The intensity of the exercises started from 30% of the weight of the rats initial and reached 100% of their weight until the end of the training. Resistance training in hypoxia. Hypoxia tent with14.4%oxygen was used to create hypoxia. 48hours after training, tissue samples were taken from horseshoe muscle and evaluated to measure the concentration of PAX7 and PGC-1α proteins.

The results showed that there is a significant difference (P=0.0001) between the research groups in both PAX7 and PGC-1α proteins. Induction of diabetes led to a significant decrease in PAX7, but the hypoxia resistance training group was not significantly different from the healthy control group (P=0.451). PGC1-α protein levels were also significantly decreased in the diabetes induction group compared to the control group (P=0.01), but training in hypoxia increased its levels to more than the healthy control group (P=0.0001).

Hypoxia, resistance training and combination of resistance training in hypoxia increased the amounts of PAX7 and PGC-1α proteins. Therefore, resistance training and temporary and inactive hypoxia exposure can be considered as a suggested solution to improve the indicators related to type2 diabetes in humans.

Nicotine alters the expression of various genes in the heart. PINK-1(PTEN-induced kinase1) is the major regulator of cellular mitophagy. Moreover, Parkin is a protein that plays a key role in the process of ubiquitination. Also, PGC-1ὰ (peroxisome proliferator-activated receptor gamma coactivator 1-alpha) is the main regulator of mitochondrial biogenesis. On the other hand, exercise has many positive physiological effects on patients suffering from heart failure. In this study, we aimed to investigate the effect of short endurance training on the expression of parkin, PINK1 and PGC- 1ὰ genes in the heart of nicotine-sensitive rats.

in this study, male Wistar rats weighing approximately 180 to 200 gr were used. The animals received nicotine at dose of 0.21 mg/kg intraperitoneally. Real time PCR technique was used to evaluate the expression of genes.

The results showed that nicotine decreased the expression of PGC-1ὰ gene (p<0.05) and had no effect on other genes (p>0.05). Short-term endurance training slightly increased the expression of all genes that was not statistically significant.

It seems that short-term exercise can reduce the pro–apoptotic and stimulant effects of oxidative stress induced by nicotine. In addition, long-term exercise may potentially induce a significant positive effect on mitophagy-related genes.

Myocardial infarction is an acute condition of myocardial necrosis. So, this study aimed the effect of high intensity interval training with curcumin supplementation on mitochondrial cardiomyocyte PGC-1α and ERR-α gene expression rats.

32 male rats were randomly divided into 4 groups of HIIT, supplement, control and HIIT with supplement training in the two groups of high intensity and HIIT with supplement included performing 8 weeks of 4-minute activity with an intensity of 85-90% VO2max. Curcumin was also used at 100 mg / kg. Mitochondrial PGC-1α and ERR-α gene expression was obtained by real-time PCR. Data were analyzed by ANOVA and Shapiro-Wilk at P≤0.05.

One-way analysis of variance test showed a significant difference in the expression of mitochondrial cardiomyocyte PGC-1α and ERR-α genes in male myocardial infarction mice in the study groups (P=0.001). The results of Tukey test showed a significant increase in PGC-1α and ERR-α gene expression in the HIIT and HIIT groups with supplementation compared to the control and supplement groups (P=0.001). There was no significant difference between the control and complement groups.

The results of this study showed that interval training alone and in interaction with curcumin increases the expression of ERRα and PGC-1α in the heart tissue of male stroke model mice. It seems that interval training and consumption of curcumin can be considered a good way to increase mitochondrial biogenesis and improve heart function.

Exercise Training and selenium is an effective way to strengthen the immune system. The purpose of this research is the effect of interval training and nano selenium supplementation on the expression of testicular HSP70 and PGC-1α genes in dexamethasone induced rats. 40 Wistar rats aged 10 weeks were randomly divided into 5 groups of 8 (healthy, under dexamethasone induction (patient), patient + interval training, patient + Nano selenium supplement, patient + interval training + consumption Nano selenium supplement) were divided. The exercise program consisted of 5-12 repetitions of 1-minute running on the rodent treadmill with an intensity of 24-30 m/min, equivalent to 75-100% of maximal oxygen consumption, with active rest intervals of 75 seconds. These exercises were performed six days a week for four weeks. The prepared selenium nanoparticle solution, 100 mg in 250 nm size, was given to mice by gavage every other day. Three-way analysis of variance was used to determine the difference in variables between groups at a significant level (P<0.05). All statistical calculations were done using Excel and SPSS version 25 software. The results obtained from the research showed that interval training led to a significant increase in HSP70 (P<0.05). Also, the expression of PGC-1α gene in the group of Nano selenium supplementation and interval training led to a significant increase (P<0.05). According to the present study, it seems that interval training and Nano selenium supplementation leads to a significant increase in HSP70 and PGC-1α, which plays an important role in improving the immune function of patients with weak immune system.

Cardiovascular disease is a major cause of death in patients with type 1 diabetes, and cardiovascular risk remains high even in patients with type 1 diabetes with good metabolic control. The aim of this study was to evaluate the effect of six weeks of aerobic exercise on inflammation and damage indicators of heart tissue in type 1 diabetic male rats.

In this experimental study, 19 male Wistar rats (mean weight 200-250 g) were randomly divided into four groups: aerobic training, sham, control and healthy. In this study, induction of type 1 diabetes was performed by injecting a single dose of streptozotocin dissolved in sodium citrate buffer intraperitoneally. Aerobic exercise program was performed with intensity of 50-60% VO2max, 5 days a week for 6 weeks. After anesthesia, an autopsy was performed and left ventricle of the heart was removed. Levels of Tumour necrosis factor α (TNF-α), Peroxisome proliferator-activated receptor-γ coactivator (PGC-1α) and Creatine kinase (CK) in rat heart tissue were measured by Western blotting. Data were analyzed by One-way ANOVA and Tukey post hoc test at the P<0.05.

  The results showed that six weeks of aerobic training led to significant decrease in TNF-α and CK and significant increase in PGC-1α of the heart tissue in type 1 diabetic rats (P<0.001).

  According to the results, it seems that aerobic training can help improve the inflammation and damage indicators of heart in type 1 diabetes.

 Due to the uncertainty of the impact of thyme plant on endurance sport performance, despite its high antioxidant property, this study aims to assess the effect of aerobic exercise combined with thyme extract supplementation on the PGC-1α gene expression in male rats.

 This experimental study was conducted on 40 adult male Wistar rats randomly classified into five groups of 8 including control, sham, aerobic exercise, thyme extract (400 mg per kg per day), and aerobic exercise + thyme extract. The rats began their exercise on a treadmill for 10 minutes a day with a speed of 10 m/min and a slope of 10%. Speed and duration of exercise gradually increased over the next two weeks until they reached 27 m/min and one hour per day, respectively. The real-time Polymerase Chain Reaction (PCR) method was used to investigate the PGC-1α gene expression extracted from the soleus muscle of rats.

 There was a significant difference in PGC-1α gene expression after intervention between the combined group and control and sham groups (Mean=1.01±0.0306; F=4.238, P=0.05). However, the PGC-1α gene expression rate in aerobic exercise group and supplementation group was no significantly different compared to control and sham groups (Mean=1.01±0.0306; F=0.00001, P=0.005).

 It seems that thyme extract supplementation along with aerobic exercise increases the PGC-1α gene expression.

Azoospermia is defined as the absence of sperm from semen. Spermatogenesis may be highly sensitive to compounds that interfere with mitochondrial energy metabolism and control of cellular respiration. The aim of this study was to evaluate the expression of SIRT1 and PGC-1α genes in testicular tissue of azoospermic rats treated with busulfan after a period of swimming training.

In this experimental study, 20 adult male wistar rats were randomly divided into four groups of healthy control: sham, azoospermia, exercise + azoospermia after azoospermia model was created. Exercise + azoospermia group, one month after azoospermia, performed low-intensity swimming exercises for 8 weeks, five days a week for 30 minutes each day. One-way analysis of variance was used to analyze the data.

The results showed that azoospermia group significantly reduced the expression level of SIRT1 and PGC-1α genes compared to healthy control group and 8 weeks of low intensity aerobic training caused a significant increase in Azoospermia + exercise group compared to azoospermia group. Has been.

In general, the results of the present study indicate that regular aerobic exercise such as low-intensity swimming in controlling the effects of infertility through the maintenance and development of mitochondrial biogenesis helps to improve the process of spermatogenesis.