جستجوی مقالات مرتبط با کلیدواژه « روی سرم » در نشریات گروه « پزشکی »

Rheumatoid arthritis is a chronic autoimmune disease characterized by an inflammatory polyarthritis that preferentially affects small joints. Some factors such as old age, systolic blood pressure and LDL, ESR, GFR are effective in the occurrence of cardiovascular diseases in these patients. The present study was conducted with the aim of determining the relationship between serum uric acid level and severity of clinical symptoms in rheumatoid arthritis patients.

In this descriptive-analytical study, the required data were collected from all the files of patients referred to rheumatology clinics of Birjand University of Medical Sciences in 2019. The investigated variables included age, ESR, CRP, RF, Anti ccp, serum uric acid level and DAS28 level of the patients. Data were analyzed with the help of SPSS version 22 software and statistical tests of Mann and Tiny, Kraskal-Wallis and Spearman's correlation coefficient. The error rate was less than 0.05.

In this study, the majority of subjects (74.4%) were women and the rest were men. The average age of the patients was 47.25±14.36. The mean serum uric acid level of the patients was 6.286±1.46. According to the results, there was a significant relationship between the serum uric acid level and the clinical symptoms of the patients (P=0.001, X2=17.271).

Considering the relationship between serum uric acid level and severity of clinical symptoms in patients with rheumatoid arthritis, it seems that this index can be used as an indicator to determine the severity of clinical symptoms in these patients.

Neisseria meningitidis is a leading cause of death in children in developed countries. Meningococcus has 13 sero-groups based on the capsule antigens. Rapid diagnosis of the bacteria results in timely selection of the right anti-microbial treatment. The present study, conducted in 2019, aimed to produce a highly pure antiserum for diagnostic assays for the detection of meningococcus sero-groups.

The type of research study was research and development. Inactivated bacterial suspension was intravenously injected into white New Zealand Rabbits. Serum was collected after the last injection. Purification process was performed using precipitation, chromatography, and tangential flow filtration. The produced antiserum was used in Agglutination and ELISA assays. The purified antiserum had a purity of more than 98% at a suitable quantity. Agglutination test with the primary antigen showed 4+ result. To develop the ELISA assay, the optimized concentration of primary and biotinylated antibodies was 1 and 80 micrograms, respectively. The results of positive predictive value were 100% and negative predictive value was 96%, for the designed assay.

The purified antiserum had a purity of more than 98% at a suitable quantity. Agglutination test with the primary antigen showed 4+ result. To developed the ELISA assay, the optimized concentration of primary and biotinylated antibodies was 1 and 80 micrograms, respectively. The results of positive predictive value were 100% and Negative predictive value was 96%, for the designed assay.

Serum-based assays are recommended for the timely diagnosis of the disease since these assays are specific, sensitive, inexpensive, and rapid. The antiserum produced in the present research is highly pure. Therefore, we recommend the antiserum for the development of agglutination and ELISA assays for the detection of N. meningitides.

Since vitamin D has been shown to have an important function in the regulation of metabolic pathways, this study aimed to investigate the association between serum levels of vitamin D with a number of metabolic indicators in patients with non-alcoholic fatty liver disease (NAFLD).

This descriptive study was conducted on 122 patients with NAFLD referred to Sheikh Alreis Clinic in Tabriz, who were categorized into "vitamin D sufficient" (n=46) and "vitamin D deficient" (n=76) based on their serum vitamin D concentrations. NAFLD was diagnosed by upper abdomen ultrasonography, and the fasting serum concentrations of glucose (FBS), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c), high- density lipoprotein cholesterol (HDL-c) as well as Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) were measured as metabolic markers. Serum concentration of liver transaminase enzymes and liver steatosis severity were assessed as liver function criteria.

Serum concentrations of insulin (p=0.024), LDL-c (p<0.001), and HOMA-IR (p=0.011) were significantly higher in the NAFLD patients with vitamin D deficiency compared to the patients with sufficient vitamin D. Moreover, vitamin D deficient patients had higher NAFLD histologic severity (p=0.042) and serum concentrations of alanine aminoteransferase (ALT) compared to the NAFLD patients with vitamin D sufficiency (p=0.005). Vitamin D-deficient patients were at higher odds for having hypercholesterolemia (p=0.009) and high LDL-c (p=0.011) than ones with sufficient vitamin D status even when adjusted for confounders.

Vitamin D deficiency in the patients with NAFLD was found to be associated with higher odds of an unfavorable lipid profile and NAFLD histologic severity.

Due to the role of insulin in the control of blood sugar and its widespread use,it is very important to fine-tune the amount of insulin that should reach the patient so that the patient is not exposed to the complications of non-regulation of blood sugar. Research has shown that the choice of solution and serum containers is effective in the patient's access to insulin.Our goal is to compare the degree of insulin adhesion to the walls of glass and plastic containers.

First,3 one-lit glass and plastic containers containing5%dextrose were prepared and300 mu of regular insulin was added to them.2cc albumin and plasma were added to glass and plastic containers No2-3.At time0,sampling was done from the end of the set,then the10cc/min flow was maintained and the sampling was repeated at 30-60min.Insulin concentration in the samples was assessed by insulin test kits and the data were analyzed by various tests.

At time 0,50% adhesion was observed in glass containers and75% in plastic containers (P<0.05).At 0-30,there was no significant difference in insulin levels between glass and plastic containers(P>0.05).Mean insulin decreased significantly(P<0.05)in glass containers during 60 min but increased(P>0.05)in plastic containers.In general, during one hour,the trend of changes in mean insulin in containers was significant(P<0.05).

The highest amount of wall adhesion occurs at time0,which is significantly higher in plastic containers than glass.after one hour,a decrease in insulin content occurs significantly in glass containers,while in plastic containers,the amount of insulin increases slightly.

Foxo1 is a transcription factor in insulin signaling in the liver. The function of Foxo1 is important in the ability of insulin to regulate hepatic glucose production and glucose homeostasis. The aim of this study was to determine the effect of resistance training program on Foxo1 expression in liver cells as well as glucose and insulin serum levels in male Wistar rats with type 2 diabetes.

The statistical population of this experimental study was 10-weeks old, male Wistar rats weighing 220±20 g (n=14). The studied rats were subjected to 6 weeks of high-fat diet and STZ injection for type 2 diabetes induction and then rats were randomly divided into two groups (control and resistance). The training group participated in a resistance training course for 6 weeks, 5 sessions per week, in the form of 4 sets and 5 repetitions per set, climbing a 26-step ladder to a height of one meter with a vertical slope of 80%. Finally, 48 hours after the last training session, fasting blood  glucose and insulin levels as well as Foxo1 expression level  in liver cells of both groups were measured and compared by independent t-test (a=%5).

The resistance training improved fasting blood glucose compared to control subjects (p>0.001). the serum insulin levels were significantly increased (p=0.042) and Foxo1 expression significantly decreased in resistance group when compared with control subjects (p=0.023).

Based on these data, the decrease in glucose concentration in the exercise group can probably be attributed to the decreased expression of Foxo1 in hepatocyte cells in response to resistance training.

Early detection of allograft dysfunction is important for the evaluation of postoperative outcome. The aim of this study was to evaluate the trend of changes in serum Creatinine (sCr) and Beta2-microglobulin (B2M) in kidney recipients (KRs) from living donors.

Blood samples were collected from KRs (n=39) at eight checkpoints starting at the day before transplantation. Based on serum creatinine (sCr; mg / dl) on the fifth day after transplantation (i.e. <1.70 or > 1.70), the KRs were classified into Good Early Graft Function (GEGF) and Poor Early Graft Function (PEGF) groups.  Demographic and clinical indicators of the recipients were extracted from hospital database. The sCr was measured by Jaffechr('39')s and serum sB2M was measured by ELISA methods. The statistical population was described using the median index. The difference between the two groups was evaluated with a significance level of 0.05 and the Independent T-test and relevant graphs were drawn with Excel 2016.

The distribution of GEGF and PEGF was 66.7% and 33.3%, respectively. In the GEGF, the sCr levels gradually decreased reaching a nadir at 36 hours after the surgery. In the PEGE, the slope for sCr elimination was slower than that of the GEGF patients.  In the case of sB2M, the trend for GEGF patients reached a nadir at 72 hours after the surgery. For the PEGF group, no changes were recorded for B2M during the follow-up.

The present study is the first to explore the diagnostic value of serum B2M in KRs from living donors. This pilot study shows that B2M is capable of identifying KRs at high risk for reduced renal function. Further studies are required to evaluate the diagnostic performance of as biomarker of allograft dysfunction.

Increased Mafa expression with increased insulin secretion from pancreatic beta cells and consequent improvement in glycemic profile in type 2 diabetic patients. This study aimed to determine the effect of resistance on Mafa expression in pancreas tissue, glucose and insulin in type 2 diabetes rats.

The statistical population consisted of all male Wistar rats of the pastor inistitue of Iran, among which 14 were male rat 10 weeks old weighting 220 ± 20 g. Type 2 diabetes induced by Intraperitoneal injection of nicotinamid + STZ. Then rats divided randomly into resistance (6 weeks, 5 times/weekly, n=7) and control (n=7) groups. Fasting glucose, serum insulin and Mafa expression in Pancreas tissue of both groups were measured after lasted exercise and compared between 2 groups by independent T test.

Resistance training significantly reduced fasting glucose and significantly increased serum insulin levels compared with the control group. Resistance training also increased Mafa expression in pancreatic tissue( p>0/0001).

Based on these data, increased serum insulin in response to resistance training in T2D rats may be attributed with increased Mafa expression in pancreas tissue.

Contrast-induced nephropathy (CIN) as a common complication of administration of contrast media (CM) is a major source of hospital morbidity and mortality. The aim of this study was to determine the incidence of CIN after contrast-enhanced CT scan and identifying the related risk factors.

A cross-sectional study was carried out in 100 patients attending Sari Imam Khomeini Hospital who underwent contrast-enhanced CT scan in 2016. Predictors of CIN were evaluated in four general categories, including demographic variables, underlying diseases, concomitant medications, and laboratory findings. Data were analyzed in SPSS V20.

In this study, increase in the concentration baseline serum creatinine of more than 25% or 0.5 mg/dl within 48 hours after the injection of contrast material was considered as an indicator of the incidence of CIN. Accordingly, the incidence rate was 7%. The incidence of CIN was significantly different in patients with and without hypertension (P= 0.028) and patients taking angiotensin II receptor blocking drugs (ARBs) (P= 0.019). Compared to creatinine, BUN and eGFR were both observed to be more accurate in predicting the incidence of CIN. BUN was found to have higher sensitivity and lower specificity while eGFR showed higher specificity and lower sensitivity.

In addition to hypertension and the use of ARBs as the major risk factors for the incidence of CIN, BUN was observed to be a better predictor for CIN than eGFR. Higher Efficacy of BUN could have been due to its larger size and therefore fewer laboratory errors.

This study aimed to investigate the changes of serum zinc in term neonates who need major operation. In this study, 59 neonates who need major operation were studied. All patients underwent major surgical with high stress. The metabolic response to surgery was studied by measuring the serum zinc before and after surgery (24 hours and at discharge time postoperatively). The "repeated measure" analysis was used to test changes of plasma values of zinc over time.  The plasma values of zinc was 89.78±14.50 µg/dl after surgery, 74.95±15.86 µg/dl in 24 hours after surgery and 93.78±14.53 µg/dl at discharge. The plasma values of zinc decreased immediately after surgery that this decline continued until the first 24 hours of operation. Serum zinc concentration decreased significantly at 24 hours at discharge. No significant differences were found between the two sex groups. According to the results of this study, zinc levels in term neonates who need major operation were decreased temporary but later returned to normal. It seems that in these patients there is no need for supplementation of zinc during two weeks after operation.

Menopause is the most critical period with unfavorable changes in anthropometric and metabolic parameters which is associated with Cardiovascular disease risk. Metabolic syndrome has a key role in causing cardiovascular disease and has been a major problem in many countries. According to various studies, the increased ferritin level is associated with metabolic syndrome and those with higher ferritin levels are at more risk of metabolic syndrome. Therefore, due to the high prevalence of metabolic syndrome in Iran, this study was performed with aim to investigate the relationship between serum ferritin levels and metabolic syndrome. This cross-sectional study was conducted on 165 menopausal women referred to the 17 Shahrivar health center and health center No. 8 of Ahvaz in 2013. Demographic information questionnaire was completed for the subjects; for the evaluation of the components of metabolic syndrome, blood pressure was measured twice with interval of 10 minutes after a break and their mean was considered. Waist circumference (WC) was measured. HDL, triglycerides, cholesterol, serum ferritin and FBS tests were done after 12 hours of night fasting on serum of patients. Data were analyzed using SPSS software (version 19) and Chi-square test, ANOVA, Pearson and Spearman correlation coefficients. P In this study, 58 patients (35.2%) had metabolic syndrome. The mean serum ferritin levels in women with metabolic syndrome were 34.53 ± 25.45 and in women without metabolic syndrome was 33.56± 23.12. There was a significant relationship between body mass index, waist circumference, blood glucose, plasma triglyceride, lipid profile and metabolic syndrome (p<0.05). But no significant relationship was found between metabolic syndrome and age, metabolic syndrome and serum ferritin (p>0.05). There is no significant relationship between serum ferritin levels and metabolic syndrome.

The aim of this study was to investigate the effect of six weeks of Pilates training with Cuminum Extract consumption on lipid profiles and insulin resistance index in overweight and obese women.   In this quasi-experimental study, 48 women with an average weight of 91.4 ± 4.7 kg and body mass index of 32.0 ± 3.3 kg/m2, were randomly divided into the four groups (n=12) of Pilates training, Pilates training and cumin, Cumin and control. Then, the experimental group performed 3, 40 minute Pilates training sessions a week for six weeks. 10 cc Cumin extract was consumed before each meal. Data analysis was done using paired t-test to compare intra-group changes and ANOVA for between group differences.   There was a significant decrease in serum triglyceride, cholesterol, LDL and insulin resistance and also a significant increase in serum HDL levels in both Pilates training and Pilates training + cumin regimen groups. Glucose level decreased significantly only in the Pilates and cumin group.   It seems that participation in Pilates training plus cumin, can improve lipid profiles and insulin resistance in obese and overweight people.

  Mortality of hemodialysis patients is still high, which can be estimated using the Charlson Comorbidity Index (CCI) and serum albumin. This prospective cohort study was conducted on adult chronic hemodialysis patients (n=532) from 9 hemodialysis (HD) facilities in September 2012. The obtained data included demographic factors and laboratory data, which consisted of serum albumin and patients’ comorbidities at the beginning of study, as well as the causes of mortality, hospitalization, and termination of hemodialysis in a 28-month follow-up period. Furthermore, one- and two-year survival of patients was evaluated by CCI and the age of patients was estimated by serum albumin≥ 3.5 g/dL and serum albumin< 3.5 g/dL. The mean age of patients was 56±15.4 years. The participants consisted of 57% men, 41% diabetes, and 43% ischemic heart disease. The mean of dialysis vintage was 44.6±49.1 months. The mean values were 10.6±1.50 g/dl, 3.90±0.35 g/dl, and 1.31±0.21 for serum hemoglobin, serum albumin, and dialysis treatment adequacy (Kt/V; i.e., K=Clearance Dialyzer, T=Time, V=Volume of Distribution of Urea), respectively. A total of 161 (30%) patients passed away (17 per 100 patient a year), and the most common causes of mortality were cardiovascular diseases (42%) and infections (25%). In Cox proportional hazard model, hazard ratio (HR) of death was 1.03 year (95% CI: 1.01-1.05; P=0.007), for serum albumin (g/dL) was 0.21 (95% CI: 0.11-0.40; P<0.001), and for CCI was 1.75 (95% CI: 1.59-1.94; P<0.001). One-year survival probability for patients in the group A (serum albumin≥ 3.5 g/dL) with CCI scores 2-3, 4-5, 6-7, and ≥ 8 were 96%, 92%, 81%, and 64%, respectively. However, these numbers for patients in group B (serum albumin< 3.5 g/dL) were 86%, 75%, 60%, and 42%, respectively. Two-year survival probability for patients in group A with CCI scores 2-3, 4-5, 6-7, and ≥ 8 were 94%, 89%, 73%, and 36%, respectively. Furthermore, the obtained results for patients in group B were 86%, 45%, 39% and 22%, respectively. Charlson Comorbidity Index along with serum albumin and age are appropriate variables to predict one- and two-year survival of HD patients. Therefore, there is a need to take appropriate measures for high risk patients to improve their health status

In patients with beta – thalassemia major, iron overload and oxidative stress cause metabolic disorders, especially in the liver. Green tea may be beneficial in reducing the complications of this disease due to the iron chelation and antioxidant properties. Therefore, this study was conducted to evaluate the effect of green tea on liver enzymes and serum lipid profiles in these patients. This randomized clinical trial was performed on 52 patients with beta – thalassemia major in two groups of controls and intervention (26 people in each group). Patients in the intervention group received 3 cups of green tea for 8 weeks and the control group consumed the same amount of boiled water each day for the same period. The assessment of dietary intake, AST and ALT, total cholesterol, triglyceride, LDL-C and HDL-C, and height and weight measurements were performed at the beginning and the end of the study. FINDINGS: Significant decrease in the levels of ALT enzymes (p<0.0001, 38.8±4.8 U/L) and AST (p<0.007, 39.53±11.01 U/L) and serum triglyceride (p<0.0001, 135.03±31.82 mg/dl) compared to baseline values (42.53±5.26 U/L, 43.46±9.7 U/L, 150.35±33.34 mg/dl) was observed in the green tea group. The consumption of green tea resulted in a significant reduction in total serum cholesterol levels at the end of the study in the intervention group compared with the control group (p=0.006). The results of this study showed that green tea consumption reduced levels of liver enzymes, triglycerides and especially total serum cholesterol in patients with beta – thalassemia major.

Ritalin has properties similar to amphetamines and is therefore used arbitrarily. The purpose of this study was to investigate the effect of ritalin on liver histology and some liver enzymes in streptozotocin-safe and diabetic rats.
This experimental study was conducted in September 2012 at Islamic Azad University, Damghan Branch, Iran. In this research, 80 male rats were divided into 8 groups of 10 rats, which included: control group consisting of healthy rats and experimental groups 1, 2 and 3 (healthy긫媖), which ritalin was taken as daily gavage 2.5 mg/kg, as well as control group (diabetic) and experimental group 4, 5 and 6 (diabetic긫媖) after 2 months of diabetic ritalin at doses of 2.5 and 5 mg/kg as daily gavages up to 30 days. At the end of the prescribed day, the rats were anesthetized and after sampling from the heart, samples were taken from the liver and samples were delivered to the laboratory.
Significant decrease in albumin levels of experimental groups compared to control group (P Oral consumption of ritalin caused a disturbance in the balance of liver enzymes and elevated serum albumin levels in healthy and diabetic rats. In the experimental groups (healthy ritalin) and (diabetic긫媖), the higher the dose of the drug, the increased levels of liver enzymes as compared to the diabetic group. Severe degrees of tissue alteration are observed in the group (diabetic긫媖). The texture of the tissue in the group (diabetic긫媖) disappeared and appeared in the texture of the disintegration.

Chronic blood transfusions are effective in preventing both initial and recurrent strokes in patients with sickle cell disease (SCD). Individuals with high iron burden are at significant risk for end organ damage and death due to cardiac and liver complications. For this reason patients receiving chronic blood transfusions are placed on the iron chelator desferrioxamine. Monitoring body iron content is critical for clinical management of patients with iron overload. The objective of this study was to evaluate if there is a correlation between serum ferritin level with the liver and heart MRI results.
Subjects and This study was a cross-sectional study based on the demographic and the patient’s laboratory and imaging results including age, sex ,serum ferritin, hemoglobin, heart and liver MRI T2*. Analysis after determining the sample size required to estimate the correlation between ferritin or hemoglobin using re-sampling method .To detect the correlation between serum ferritin and MRIT2* heart or liver in sickle cell disease the Pearson and Spearman correlation coefficient was used.
Although, there was no significant correlation between hemoglobin and heart and liver MRI T2*, but there was significantly inverse correlation between serum ferritin and heart and liver MRI T2* in sickle cell anemia patients.
To better evaluate the iron overload of liver and heart in sickle cell anemia patients, measurement of serum ferritin or MRI T2* is suggested and can be used instead of each other in some circumstances.

Nursing is one of professions that employees are at risk of fatigue and work related musculoskeletal disorders, because of high physical workload and high job stress. Connecting serum set to serum solution is one of the repetitive tasks for nurses in their working times and it may cause pain and discomfort in their hand. The aim of this study was to design and ergonomic evaluation of a tool for connecting serum set to serum solution.
Material and This experimental- interventional study conducted on 12 nurses (6 men and 6 women) in 3 phases. First phase was assessment of manual connecting of serum set to the bag by nurses, second phase was design and manufacturing of serum set connector and third was ergonomic assessment of the manufactured tool. In first and third phases, amount of perceived exertion by nurses was assessed using the Burg scale (CR10), and electromyography assessment for hand muscles activity was conducted during connecting serum set. Data of first and third phases compared with statistical tests.
Mean score of perceived effort during manual connection of serum set was 5±1/2 (of 10) and while mechanical connection with designed tool was 2.3±0/49. There was significant difference between activity in 5 muscles (Flexor Digit Comonis, Flexor Carpi Radial, Biceps, Triceps and Deltoid) in manually and mechanically connection mode (P-value Use of serum set connector reduced the perceived effort and activity of hand and wrist muscles. This device can be used as an ergonomic tool for nurses to easing the inserting the serum set to serum solution

Artificial sweeteners are chemical or natural substances used in foods and beverages. The aim of this study was to compare the glycemic control status before and after the use of artificial sweeteners with conventional nutrition in type 2 diabetic patients.
This quasi-experimental study was performed on 30 patients with type 2 diabetes (10 males and 20 females) whom referred to the endocrinology clinic in Tabriz in west of Iran. At the beginning of the study, patients were asked to refrain from using dietary supplements containing artificial sweeteners for three months and continue their usual diabetic diet. Then, each patient was received nutritional supplements containing artificial sweeteners including sugar, special biscuits and candy under a dietitian for three months.
Mean glucose level of fasting serum and serum glucose 2 hours after meals in patients during the second trimester increased significantly with the use of artificial sweeteners compared to the first three months (P The use of artificial sweeteners in diabetic patients may result serum glucose increase.

Diabetes mellitus is a metabolic disorder that is characterized by hyperglycemia resulting from defects in insulin secretion and action, or even both of them. Proveskia abrotanoides has anti-bacterial, anti-parasitic, antioxidant, anti-inflammatory, and analgesic effects. This study was done to evaluate the effect of hydro-alcoholic extract of Proveskia abrotanoides on blood glucose and liver enzymes level in streptozotocin-induced diabetic rats.
In this experimental study, 60 male Wistar rats were randomly allocated into including healthy control, healthy received Glibenclamide, healthy -treated with 150, 300 and 600 mg/kg/bw of Proveskia abrotanoides extract, diabetic control, diabetic treated with 150, 300 and 600 mg/kg/bw of extract, positive control (diabetic treated with the Glibenclamide). After the treatments, the blood samples were taken from the animals and the level of blood glucose and liver enzymes including ALT, AST, and ALP were measured. Finally, the effect of hydro-alcoholic extract of Proveskia abrotanoides was compared with Glibenclamide as a conventional drug.
The results showed a significant increase in liver enzymes (ALT, AST, ALP) in hyperglycemic rats compared to the healthy controls (P The hydro-alcoholic extract of Proveskia abrotanoides reduces liver enzymes and blood glucose level in streptozotocin-induced diabetic rats.

The relationship between dietary patterns and esophageal cancer has not been documented yet. However, recent studies have shown that the Western dietary pattern is associated with an increased risk of esophageal cancer. The current study was performed a comparative evaluation of the food habits and serum nitrate level in patients with esophageal cancer and healthy individuals in Gorgan city.
In a case-control study, 44 patients with esophageal cancer (the case group) and 44 healthy subjects (control group) were selected for one year in Gorgan city. Anthropometric factors were measured, and then demographic and dietary data were recorded using general and food frequency questionnaires (FFQs), respectively. Fasting blood samples from both groups were collected to measure serum nitrate levels. Data were analyzed by independent t-test, Chi-square, Fisher, and Monte Carlo tests.
the mean weight, body mass index (BMI) and serum nitrate levels were statistically lower in the case group than in control group (p Consumption of fast foods, hot tea and saturated fats may be associated with esophageal cancer. Serum nitrate levels were lower in patients than control group, probably indicating the need for further research in this field.