جستجوی مقالات مرتبط با کلیدواژه « تخمدان » در نشریات گروه « دامپزشکی »

Protection from reproductive damage is essential in chemotherapy medicines for cancer patients. This study aims to examine the effect of curcumin on the structure of the ovary of mice after treatment with goserelin and cyclophosphamide. One hundred and ten BALB/C mice with 3 regular consecutive periods of the estrous cycle were divided into 11 groups of 10 each. No medicine was used in the control group. The treatment groups were as follows: 1) cyclophosphamide, 2 to 5) cyclophosphamide with curcumin with a dose of 100, 200, 300, and 400 mg/kg, respectively, 6) goserelin, 7 to 10) goserelin together with curcumin with a dose 100, 200, 300, 400 mg/kg, respectively. The luteinizing hormone (LH) and follicle-stimulating hormone (FSH) of serums were evaluated using ELISA. Morphologic and morphometric of ovaries were assessed. The total number of follicles, primary, secondary, periantral, and antral follicles, in the goserelin and cyclophosphamide group, was significantly reduced compared with the control group (P<0.05). Cyclophosphamide and goserelin with different doses of curcumin showed a significant increase in the total number of follicles, primary, periantral, and antral follicles compared to the group treated with cyclophosphamide and goserelin alone (P<0.05). Curcumin (200, 300, and 400 mg/kg) and cyclophosphamide, compared to the cyclophosphamide group, significantly increased the quality of zona pellucida (P<0.05). Cyclophosphamide and goserelin caused a significant decrease in FSH and LH (P<0.05). Cyclophosphamide with different doses of curcumin showed a significant increase in LH compared to the group treated with cyclophosphamide alone (P<0.05). Goserelin with a 400 mg/kg curcumin dose significantly increased LH compared to goserelin alone (P<0.05). The amount of FSH in the cyclophosphamide groups with curcumin increased compared considerably to cyclophosphamide alone (P<0.05). The groups of goserelin with curcumin showed a significant increase in FSH compared to those of goserelin alone (P<0.05). Curcumin can protect the reproductive system of mice from the damage caused by the administration of cyclophosphamide and goserelin.

Exposure to nicotine and cigarette smoke has dangerous effects on pregnant women, fetal and neonatal health, and fertility rates. Aloe vera is one of the few medicinal plants that has maintained its popularity for a long time due to its many medicinal properties. Therefore, in the present study, the protective effect of aloe vera gel on ovarian histological changes in nicotine-receiving rats was investigated. For this purpose, 32 female Wistar rats into 4 control groups (normal diet), nicotine (6 weeks nicotine at 0.2 mg / kg body weight of animals injected intra peritoneal), nicotine-aloe vera (6 weeks nicotine injection + gel) Aloe vera plant (400 mg / kg body weight of animals orally), Aloe vera group (6 weeks aloe vera gel by the above method and amount) were divided. At the end of the course, the mice were facilitated and the ovaries were transferred to a histological laboratory to prepare a tissue slide and perform hematoxylin-eosin staining. The results showed that after nicotine treatment, weight loss, ovarian weight, number of healthy and active follicles, new corpus luteum and increase in the number of atretic follicles were observed. But the use of aloe vera gel was able to improve all the microscopic complications and disorders and macroscopic changes caused by nicotine injection. Therefore, oral consumption of aloe vera gel can reduce or inhibit the harmful effects of nicotine in tobacco or drugs on the ovaries and fertility in women.

Effect of nicotine on male and female mammal fertility capability. Nicotine decrease reproduction and cause infertility in each sex. It causes disorder in luteal phase by inhibition of release progesterone. In present study cellular effects of nicotine were studied on rat ovary by histochemichal methods.  Fifteen rats were selected as three groups which each group contained five animals. Control group, once treatment samples received low dose nicotine (0.2 Mg/Kg) daily and second treatment samples received high dose nicotine (0.4 Mg/Kg) daily during twenty one days. injection was given intraperitoneally. rats were killed by carbon dioxide and ovaries were removed and frozen, and then were sectioned by cry microtome with thickness 10 µm were prepared. stained by Oil-Red-O, Sudan Black B and PAS method. And were examined by light microscope. Results appeared that, in low dose group follicular fluid, some of the growing follicles, and cystic follicles were PAS positive. All of the positive effects were observed in high dose group too but accuracy of follicle degeneracies was more in this group. Obtained results appeared many Oil-Red-O positive areas in treated ovaries that it guidance cell degeneration and unused cell energy source. Many distributed Oil-Red-o and PAS Positive area in treated group appeared that nicotine cause cellular degeneration. Therefore degenerative effects of nicotine on ovary can be  cause infertility in female animals.

With the expansion of industrial processes, heavy metal pollution for example lead has become a serious problem. they have the ability to pass through organs and causes them to malfunction. One of the target organs of lead is ovarian tissue. Lead is also able to cross the blood-placental barrier and transferred to the fetus. It is also possible to pass lead through milk to the newborn. This study was performed to investigate the effect of low dose mother's lead Consumption on the development of the ovaries using stereology technique in rats as animal models. In the present study, Wistar rats were randomly divided into five groups. The groups included the Control group and 4 experimental groups of Pre-pregnancy, Pregnancy, Lactation and Pre-pregnancy-pregnancy-lactation. The experimental groups received 0.2% lead acetate, with 0.5cc glacial acetic acid– through drinking water. Acetate consumption was in such a way that in the pre-pregnancy group 30 days before mating, in the pregnancy group during 21 days of pregnancy, in the lactation group during 21 days of lactation, and in the pre-pregnancy-lactation group from 30 days before mating to the end of lactation they received lead acetate through drinking water. On the 65th day after the birth, all infants were killed in the laboratory and left ovarian specimens were collected for stereological studies. Examination of the results showed that the total volume of ovary, cortex, medulla, interstitial tissue and total volume of adult reproductive follicles were not significantly different between the experimental groups of pre-pregnancy, pregnancy, lactation and pre-pregnancy, pregnancy-lactation and the control group. Examination of the corpus luteum volume results showed that the pre-pregnancy group had a significant decrease compared to the pregnancy group. The present study showed that exposure of mothers to low-dose lead acetate reduces the volume of corpus luteum, especially during pre-pregnancy.

The laying hen possesses a single functional ovary, which undergoes ovulation at a high rate during its lifespan. In hens, spontaneous tumors of the ovary and, less frequently, of the oviduct, are common. More than 80% of commercial laying hens develop ovarian tumors by the time they are 2 years of age. Ovarian tumors usually don't become apparent until their growth is well advanced. The bird may have a history of egg binding or oviductual prolapse. They may also have problems associated with egg laying, such as increased infections, soft-shelled, shell-less and other abnormal eggs, and generalized signs of lethargy/depression. There is no available cure for ovarian tumors in hens. Treatment options are aimed at stopping egg production and to minimize risk of future egg-related infections such as egg yolk peritonitis. These include hormonal implants or, as a last resort, "spaying". There are also a number of different natural herbs which have shown some benefit. It showed that hens fed a flaxseed-enriched diet for one year experienced a significant reduction in late-stage ovarian tumors. Reproductive tumors can reduce egg production rates and lead to an overall decline in general health in the laying hen. Usually viral agents such as: herpes and retro viruses cause tumors in birds. Most researches on avian tumors has been done in viral agents cases, because of their economic importance and because these studies serve as a model for cancer studies in women. Ovarian epithelial cancer is the fifth leading cause of cancer deaths and the most deadly reproductive tract cancer in women. Domestic poultry is the most appropriate animal model for studying ovarian cancer in women. No other animal develops spontaneous ovarian tumors at comparable rates to the chicken which can exceed 35% depending on the genetic background (i.e., strain, age, and number of eggs produced by the flock. Moreover, the pathology and progression of the disease resembles that in humans in several respects. Leiomyoma and oviduct adenocarcinoma are the most common tumors of the reproductive tract in laying hens. Oviduct adenocarcinomas usually originate from the magnum of the oviduct, and occasionally arise from the infundibulum and uterus. Adenocarcinomas in poultry are among the groups of unknown etiology tumors, but a relationship between high egg production and the prevalence of oviduct tumors has been shown. Neoplastic cells are shed from tumors in the oviduct into the abdominal cavity. They implant on the ovary, pancreas, and other viscera and produce multiple, hard, yellow nodules.

The present communication, reports a metastatic adenocarcinoma in an 80 week old commercial layer chicken in second-cycle egg production in Mazandaran province, Iran. Eight dead layer hens were referred to division of avian diseases. On necropsy examination, mesentery and serosal surface of intestine was studded with grayish white nodules. Ovary contained varying sizes of multiple single to lobulated firm, cauliflower-like, grayish white nodules. Also, yellowish transparent ascites were seen in some cases. Tissue samples were taken and placed in 10% neutral buffered formalin, then, were placed in cassettes, dehydrated in ethanol, cleared with xylene, and embedded in paraffin wax. Sections were made at 5 µm thickness on glass microscope slides and stained with standard haematoxylin and eosin (H&E) and then examined by light microscopy.

Microscopically, cuboidal neoplastic cells were arranged in acinar and tubular pattern in the serosal layer of intestine. The cells appeared as variable sized cuboidal cells with indistinct cell borders, eosinophilic granular cytoplasm, round nuclei, indistinct nucleoli and fine chromatin granules. Intestine nodules showed neoplastic cells and structures similar to those of the ovary tumor. These adenocarcinomas were encountered in 80-week-old White Leghorn hens, which tallies with the reviewed published findings that ovarian and oviductal adenocarcinomas are among the most frequently encountered tumors in older White Leghorn hens. Laying hens are the only animals that develop spontaneous ovarian cancer similar to humans. In the present case, according to histological appearance of the growth in the ovary, the tumor was diagnosed as an ovarian adenocarcinoma with metastasis on the whole of intestines. In this study, ascites was observed in the abdominal cavity of birds; the tumor obstructed the lymph flow and thus could lead to ascites. Prolonged egg production period may be one of the important causes of involvement with this type of tumor. Continuous secretion of gonadotropins can over-stimulate estrogen-sensitive epithelial cells in the oviduct and cause tumor cells to develop.

Neoplastic diseases, regardless of their cause or the organ affected, have economically impact; therefore, it is important to report these cases.Keywords: Layer chicken, Ovary, Intestine, Adenocarcinoma, Metastasis.

This study aimed to investigate the gonadal tissue structure and sexual hormones in the yellowfin seabream (Acanthopagrus latus) and sole (Euryglossa orientalis) collected from the Mussa creek. For this purpose, about 1200 fish (10fish/species/station) were collected from five selected stations in Mussa creek including Petrochemical, Gafari, Majidieh, Ghazaleh and Zangi stations  and Genaveh Port station (as control). After the biometrical assay, bleeding was conducted from the fish caudal vine and blood samples were moved to heparinized microtubes and serum was separated after centrifuging. Also, tissue samples were taken from the ovary and fixed in formalin. Tissue samples passed through a routine histological process and the tissue sections were prepared and stained with H&E. later, histological sections were studied under light microscope and microphotographs were prepared using Dino lite with Dinocapture software. The steroid hormones including 17-βestradiol (E2) and testosterone (T) were measured using the radioimmuneassay (RIA) method and gonadotropin hormones (GTHI and GTHII) were also measured by IRMA method. The most follicles observed in the ovarian samples of both species from different stations were in previtellogenic stages including chromatin-nucleolus, prenucleolus and cortical alveoli follicles. Also an increase in the interstitial connective tissue and atretic follicles were recorded in the ovarian samples from some stations especially Petrochemical station. The results showed that the level of steroids (E2 and T) was lower in fish from the Petrochemical station than others, while GTHs reached the highest level in these fish. Generally, the most alterations in gonadal tissue structure in both fish species were recorded in the petrochemical station, probably due to waste waters released into the water in this station from various petrochemical and color alkali industries surrounded this station.

The effects of lead toxicity, even at low levels, have several disabilities, such as weakness, impaired ability and intellectual behavior of children, loss of hearing and vision, reduced sperm quality and fertility in women. On the other hand, treatments by medicinal herbs such as wheat germ, which is rich in antioxidants such as E, thiamine, zinc, phenolic acids, alkylaryrosuronquids, aminophenols, and aminobenzoic acids, can be considered. Take up 30 adult male Wistar rats (240 ± 20 g) in 6 groups: control (without treatment), 10 mg lead, hydroalcoholic extract of wheat germ with doses of 100 and 200 mg, lead in conjunction with hydroalcoholic extract of wheat germ with Doses of 100 and 200 mg were divided. On day 36, blood samples were taken and the antioxidant properties of the LH and FSH hormones and estrogen levels were evaluated. Rat weight and ovarian weight decreased in the induction group with a dose of 10 mg. Also, the concentration of estrogen, LH and FSH and antioxidant activity in the lead group decreased with different amounts, and in the hydroalcoholic extract group, wheat germ increased.

Hydroalcoholic extract of wheat germ has the ability to compensate for lead-induced lesions, which depends on the dose of prescription.

The purpose of the current study is the histomorphologic survey of Litopenaeus vannamei shrimps ovarian development is different stages of the reproductive cycle.30 female L. vannamei shrimps from ChoebdehsCultivation and Breeding Center in Abadan city were examined. Sampling was done in the spring (May 2013). The samples were transfered to the histology lab of Khorramshahr University of Marine Science and Technology. After tissue passage, tissue sections were made from them and were stained. The results of this investigation showed that the L. vannamei shrimps have asynchronous ovary, also four stages of ovarian development can be identified in this species of shrimp. In addition, stages of follicular development were divided into five stages including chromatin nucleolus, peri nucleolus, cortical alveolus, primary vitellogenesis and final vitellogenesis. Cortical alveolus stage was rarely observed. It seems that in ovary of Litopenaeus vannamei, peri nucleolus follicles quickly entered to first stage of vitellogenesis. It was also shown that ovaries can be divided into four phases morphologically.

The most common metal used in the body is iron and since it may produce ROS, it could potentially be a dangerous substance.
Regarding the side effects of nanomaterials such as nano iron particles and possibility of environmental contamination including air pollution with nanoscale iron particle, this study was designed to compare the effects of conventional iron oxide with iron oxide nanoparticles, on certain indexes of ovarian tissue in a rat experimental model.
This animal model was carried out in 5 groups of female rats, including control, iron oxide (15 mg) and iron oxide nanoparticles (5, 15 and 30 mg). The drugs were intraperitoneally injected daily for 16 days. On the seventeenth day the rats were euthanized by chloroform. Ovarian tissue was removed, and histological changes and iron accumulation were assessed by special staining and light microscopy.
According to our findings, folliculogenesis was decreased in all groups receiving iron. The number of corpus luteum in the groups receiving different doses of nanoparticle was reduced and the number of atretic follicles was significantly increased in all groups compared to the control group.
In conclusion, probably iron nanoparticles with impaired cellular oxidative pathways, reduces the number of follicles and corpus luteum and increase atretic follicles by producing oxygen free radicals and destructing microfilaments. This can cause a negative effect on the fertility of female rats.

An infertility cause in women is diabetes. One of the organs that diabetes has adverse effects on its structure and function is ovary. Nano zinc oxide (nZnO) is the one of zinc derivatives that has been mentioned as a potent anti-diabetic agent in recent studies. Moreover, zinc is an important element in fertility. Thus, at the present study nZnO effects on ovary in diabetic rats has been compared with zinc oxide. Fourty adult female wistar rats were randomly divided into 5 groups as control, sham (normal saline), diabetic (STZ=60 mg/kg, IP), Diabetic receiving ZnO (30 mg/kg) and diabetic receiving nZnO (30 mg/kg). After treatment for 4 weeks, every animal blood glucose and body weight were measured. Following euthanasia, the ovaries were isolated and floated in appropriate fixative. Five µm sections were prepared as routine histotechnique and stained and studied by light microscopy. Diabetes reduced primordial follicles and corpus luteum numbers (P

Red hot pepper is a plant that belongs to the Solanaceae family and is known as Capsicum annuum. Capsaicin is the active ingredient of cayenne pepper. Ghrelin is a hormone, which consists of polypeptide structure. Ghrelin also contributes to growth hormone secretion, energy balance, food intake and body weight regulator. The aim of this study was the localization and expression of ghrelin in the ovaries of rats treated with capsaicin during the postnatal development. Ninety female Sprague-Dawley rats (21 d) were used. The rats were randomly divided into 3 groups (n=30 each) as pubertal, post pubertal and adult. Each group was subdivided into three groups. The first subgroup (control) was given no injections. The second subgroup (vehicle) received only 0.3 cc solvent and the third subgroup (experiment) received subcutaneous injection of equal volume of capsaicin (1 mg/kg/d) for 42, 56, and 70 days. Ghrelin immunoreactivity was determined in ovarian follicular granulosa cells, interstitial cells and corpus luteal cells. A ghrelin immunopositive reaction located in the cytoplasm of cells in all groups. These results indicate that prolonged administration of low dose capsaicin does not affect ghrelin expression. However, follicular atresia was seen in lower rate in capsaicin treated group in comparison to other groups.

Maturity of silver carp (Hypophthalmichthys molitrix) ovary takes place at 2-8 years old and it is depends on photoperiod and environmental water temperature. The aim of this study was to identify macroscopical and microscopical structure of silver carp ovary in two categories of body weight, total body length and at 2 and 4 years old in Khuzestan province climatic conditions. 20 silver carp ovaries were studied in two groups: Group 1(G1): 10 fish with total body length 46 ±1.33 Cm and weight 1050±0.09 grand approximately 2 years old. Group 2(G1): 10 fish with total body length 86±3.64 Cm and weight 5460±800 gr and approximately 4 years old. For the histometrical studies, 5-6 µ sections were made by routine paraffin embedding method and stained by H&E and PAS. Microscopic results showed that ovaries of G1 covered by a thin loose connective capsule which numerous of egg-bearingtrabecula extended from capsule into ovaries. These trabecula contained primary germ cells, oogonium, chromatin nocleolus, perinocleolus and cortical alveolus follicles. Ovaries of G1 do not have yolk and mature follicles. But in ovaries of G2, growth and mature follicles were observed and mature follicles were most follicles. Histometrical results showed that there are not a significant differences in diameter of follicles, oocytes and oocytes nucleolus in G1 and 2. The maximum and minimum diameter of follicles were seen in mature and chromatin nucleolus follicles respectively. The Zona plucida thickness was increased from corticalalveolus (1.53±1.11 mm) to mature follicles (6.88±0.21 mm) significantly (p<0.05). The most interesting finding of this study is that in Khuzestan Province climate, the ovary of silver carp fish in G1, with average weight 1050 ±0.09 gr and total length 46±1.33 Cm and approximately of 2 years old is immature but ovary of G2 with average weight of 5460± 800 gr and total length 86±3.64 Cm and approximately 4 years old is mature.

Ovary and ovarian bursa of twelve female reproductive organ، six samples from each immature and mature buffaloes were collected from slaughterhouse. All samples were transferred to anatomy department of veterinary college، and they studied morphologically and morphometrically، length، wide، and thickness of ovaries، deep and wide of opening of ovarian bursa were measured. Additionally، dimension of corpus luteum and weight of ovaries along with corpus luteum were measured. This study revealed that ovaries in buffaloes are oval shape and it is briefly flattened in two sides، and surfaces of ovaries were smooth and shinny، and corpus luteum has meaty appearance too. Dimension of right ovaries without corpus luteum were more than the left ovaries. Dimension and weight of corpus luteum on the right ovaries were more than the left ovaries. Deep and wide of the right ovarian bursa was more than the left ovarian bursa، and in overall، dimension of ovarian bursa was larger than the ovaries. Difference of dimension and weight of ovaries in immature and mature buffaloes were high significant (P<0. 01)، and in overall، dimension of ovaries in immature buffaloes were 1. 54 cm length، 0. 87 cm wide، 0. 67 cm thickness، and in mature buffaloes were 2. 08 cm length، 1. 31 cm wide، 1. 07cm thickness، and weight of the ovaries in immature buffaloes were 1. 25gr، and in adult buffaloes were 3. 24gr.