جستجوی مقالات مرتبط با کلیدواژه « سگ » در نشریات گروه « دامپزشکی »

The Middle-East, known-to-be the birthplace for animal domestication, is among the few remained geographical theaters in the world where glanders-stricken solipede and human cases are still reported. In the Persian environment with some 60 years of maleination test history in its horse, mule and donkey populations, mini or midi outbreaks of glanders are seen now and then mostly in the Western and central regions. This work was aimed to characterize the phenotypic and genotypic properties of the most recently-collected isolates of Burkholderia mallei in Iran (A tiger sample and 3 horse samples). Bacterial culture, Biochemical tests, complement fixation test and Western blot, Flip 407-PCR, Bim A-PCR and also Strauss reaction tests were included in the assessment. The results obtained from experiments conducted on four isolates from Tehran, Kordan, Oshnavieh and Semirum outbreaks, displayed the expected characteristics of B. mallei. Whether our isolates of interest represent the local/regional population(s) of B. mallei, we assume further molecular epidemiology studies will enable us to address.

Canine parvovirus (CPV-2) is an important disease with high relatively prevalence. In the present study, the ultrasonographic and radiographic findings of the gastro-intestinal tract were detected in dogs suffering from hemorrhagic and non-hemorrhagic diarrhea. Thirty dogs between 2 until 11 months of age (mean= 5.43 months) were examined with confirmed canine parvovirus enteritis by immunochromatography assay (ICA). The studied dogs were categorized into three groups and each group was divided into subgroups (less than 6 months) and (above 6 months). The mean of wall thickness of stomach, small intestine and colon were recorded 1.38, 2.14 and 0.9 mm for group A1 and 1.5, 1.96 and 0.84 mm for group A2 respectively. The above parameters were detected for groups B1, B2, C1 and C2. The mean of peristaltic contractions in small intestine of different groups A1, A2, B1, B2, C1 and C2 were recorded 1.6, 1.4, 2.18, 1.5, 2.1 and 2.4 respectively. The statistical analysis showed a significant difference between groups A and B with C group (p<0.05), but the difference was not significant between groups A with B (p>0.05). The obtained results showed that hemorrhagic diarrheas (parvovirus and non-parvovirus) can decrease the number of peristaltic contractions in small intestine. Changes in echogensity were seen as hyperechoic (6 out of 30; 20%) and hypoechoic (5 out of 30; 16.67%). Although none of the above changes are pathognomonic for canine parvovirus enteritis, but the above findings in combination with other signs can be highly helpful in the treatment process.

The storage process significantly affects the quality of dog semen because the dog's semen membrane is very sensitive to this process. In addition, this process leads to the production of free radicals and, as a result, to the peroxidation of the sperm membrane. The aim of the present study was to minimize the harmful effects of free oxygen radicals by adding different concentrations of docosahexaenoic acid to dog semen at 5 °C for five days. In this study, 25 ejaculates were collected three mixed-breed dogs and diluted in a Tris-based extender. Then they were divided into 5 parts into the control groups (without antioxidants) and amounts of 1.25, 2.5, 5 and 10 ng/mL docosahexaenoic acid in the extender. The evaluations (motility, morphology, DNA integrity, viability and total antioxidant capacity) were carried out after 0, 24, 48, 72 and 120 hours during storage at cool temperatures using CASA software and a light microscope for sperm parameters and using the ELISA method for the total amount of antioxidant capacity performed. The results showed that the groups containing docosahexaenoic acid had a significant increase in the percentage of cell viability, survival and total antioxidant capacity, as well as a significant increase in DNA integrity at 24, 48, 72 and 120 hours compared to the control group groups compared to the control group. became. The proportion of sperm with normal morphology increased significantly after 120 hours compared to the control group.

Parvovirus infection is one of the deadliest viral diseases in dogs that cub and young dogs are much more involved. This disease is known as dysentery and involves the intestines and bone marrow, causing diarrhea and vomiting and reducing the body's ability to fight infection. The complications of this disease in cubs are much more severe than adult dogs. Creatinine and urea, especially creatinine, are two important indicators of kidney health. Therefore, in the present study, the effect of parvovirus on kidney was investigated by measuring these parameters.

In order to conduct this study, we selected 10 male dogs with an average age of 4-6 months with clinical and laboratory symptoms of parvovirus as the patient group. 10 seemingly healthy male dogs with an average age of 4-6 months were selected as the control group. Blood samples were taken from patients in sterile conditions before treatment. Creatinine and urea were measured by calorimetric method using spectrophotometer.

The results of the present study showed that there was a significant difference between the mean of urea and creatinine in healthy dogs and parvovirus patients (P<0.05). This difference was due to the increase in the amount of these parameters in dogs with parvovirus, indicating kidney disease in dogs with parvovirus.

In general, the results of this study showed that parvovirus, in addition to the complications that have already been proved for it, can cause renal failure in dogs and the effect of this virus on dog kidney should be considered in diagnosis and treatment of this disease.

Changes in Gastric Emptying Time, are common complications that occur due to diseases, such as eating foreign body, obstruction, nervous problems and diabetes mellitus. Radiography with contrast media is one of the common methods for diagnosing gastric tract problems such as obstructions and delay in gastric emptying. This study has examined the gastrokinetic property of Domperidone by investigating its effect on gastric emptying time.

7 mixed breed dogs, after clinical examinations, were selected from around Karaj City, Alborz Province, Iran. After a week of quarantine in standard conditions, as a control group, they were subjected to a radiographic study of the stomach using barium sulfate contrast media. After another week in quarantine and the aforementioned conditions, the same dogs were studied as a test group to prevent individual differences. For this purpose, 120 minutes before the start of the radiographic study, all dogs were given Domperidone at a dose of 5 mg per dog.

The average of the start of the Gastric Emptying Time in control and test groups were 25.71 and 12.86 minutes, respectively. The average of the end of the Gastric Emptying Time in control and test groups were measured as 94.29 and 55.71 minutes, respectively. Statistical analysis comparing the averages showed that there is a significant difference between the start of the Gastric Emptying Time in the control and test groups (P<0.05); However, there was no significant difference between the end of the Gastric Emptying Time in the control and test groups (P>0.05).

Although Domperidone accelerates the start of the Gastric Emptying Time in dogs, it does not make a significant difference at the end of the Gastric Emptying Time.

A sixteen-month-old male Ghadrejani dog was referred to the veterinary clinic with a swelling mass on the first finger of the right hand. Clinical examination revealed swelling, erythema and alopecia with ulceration. CBC, radiographs of the affected area and chest, and regional lymph node examination were reported as normal. Histopathological examination revealed a subcutaneous, multilobular, unencapsulated but well-circumscribed tumor with a fingerprint pattern of spindle cells arranged around a central capillary. Immunohistochemical analysis showed that the neoplastic cells expressed vimentin and did not express α-smooth muscle actin (SMA) and S100 protein. Based on the histopathological and immunohistochemical findings, the tumor was diagnosed as canine hemangiopericytoma (CHP). This is the first report of CHP in a young indigenous Ghadrejani dog. This report demonstrates that not only can CHP occur in young dogs, but that differentiation from other soft tissue sarcomas requires not only histopathological findings but also immunohistochemical examination.

Canine parvovirus (CPV) is one of the most contagious viral agent's causing acute enteritis in young canines with high mortality rate. This study was conducted to investigate the risk factors associated with the occurrence of CPV in dogs using a matched case-control method. The target population was dogs under one year of age referred to veterinary clinics located in South, North, and Razavi Khorasan provinces. The case group (100 dogs) had clinical symptoms of CPV disease and positive PCR test, the control group (100 dogs) had no clinical symptoms, were healthy and the PCR test was negative. Statistical analysis was performed using a multivariable logistic regression model using SPSS software. Based on the obtained results, it was determined that large breeds have a higher chance of CPV than small breeds (OR = 2.56, P = 0.004). Lack of vaccination is a risk factor in the occurrence of CPV with an equal odds ratio (OR = 2.63, P = 0.002). Dogs with homemade food had a lower chance of disease (OR = 0.26, P = 0.01), and the disease was significantly higher in shelter dogs than in domestic dogs (OR = 9.89, P = 0.0001). Dogs that were in contact with other dogs also had a higher chance of developing CPV than dogs that had no contact (OR = 3.01, P = 0.001). Therefore, the awareness of the owners regarding the vaccination of dogs at the appropriate time and preventive care regarding the interactions of dogs is essential to prevent CPV.

Campylobacter bacteria is the main cause of campylobacteriosis disease in human and animals. Pet animals include dogs and cats may be carriers of contamination and transmitted bacteria without clinical symptoms.considering the increasing desire of people to keep dogs and cats at home,the purpose of this research was to determine the frequency of campylobacter infection in pets and their owners. In this research,122 feces samples of pets and owners analyzed with microbial and molecular methods. In the microbial method,the frequency of campylobacter infection in cats and dogs was derermined to be8 and 12% respectively, and the molecular method,determined to be 36 and 44%. In the owners of pets,the frequency of infection was determined to be 20 and 32%respectively,while campylobacter infection was not observed in people unrelated to pets.Campylobacter jejuni was the most common species in all case of campylobacter infection.the result of the chi square test(pearson test)showed a significant relationship between the infection of pets and infection oftheir owners(p<0.01 ).Finally,due to the significant frequency of campylobacter infection in pets and considering the role they can play for the health of human societies periodic test of very important.

Reports in the literature indicate that species of Hepatozoon canis blood parasites are common in Iranian dog populations. In this study, we aimed to investigate the prevalence of H. canis infections and their phylogenetic relationships in dogs from an animal shelter in Guilan province using polymerase chain reaction (PCR) and sequence analysis.A total of 20 blood samples were subjected to PCR to amplify a fragment of 562 bp from the 18S rRNA gene of Hepatozoonspp. The PCR results showed that the Hepatozoon infection rate in the dogs was 10%. (2/20). Sequence analysis revealed that the H. canis found in this study was 100% matched to H. canisin in the NCBI GenBank database, which originated from a dog in Tehran (accession number KX880505) and a golden jackal in Romania (accession number KX712126). In the phylogenetic analysis, these three sequences are grouped in the same clade. This was the first study of dogs from Guilan province using molecular phylogenetic methods. There is a need for more comprehensive studies to determine the infection status in Iran.

Brucellosis caused by Brucella melitensis is one of the important health and economic diseases in Iran is very important to pay attention to it as a zoonotic disease. The aim of the study was to investigate B. melitensis infection in a kennel dog and also to compare different laboratory methods to diagnose Brucella melitensis infection in dogs. In this study, the whole blood samples (for PCR and culture), vaginal swabs samples (for PCR tests) and serum samples (for Rose Bengal, Wright and 2 mercaptoethanol tests) were obtained from 14 bitches at a kennel, in which a bitch had been aborted due to Brucella melitensis infection. Also, the whole blood sample and semen sample were obtained from the only male dog of this kennel for PCR test. Finally, the data obtained from these tests were analyzed by calculating the sensitivity and specificity as well as the Kappa coefficient. Bacteriological culture results of all blood samples were negative. The PCR results of seven blood samples (%50) and eight vaginal swab samples (%57.1) obtained from 14 bitches were positive. By using PCR primers, B. melitensis were identified in all positive samples. In the serological test, out of 14 sera obtained from bitches for the Rose Bengal screening test, eight positive samples were observed, but only one positive sample was observed in Wright and 2 mercaptoethanol tests. The results showed that the sensitivity and specificity of PCR test in vaginal swab samples was 100% and there is an excellent performance (kappa coefficient = 1) between the results of the Rose Bengal test and PCR of vaginal swab samples in the diagnosis of Brucella melitensis in dogs. In dogs suspected of brucellosis, at first it is recommended to take a sample of the animal's blood to perform the Rose Bengal test, and if the result is positive, to confirm the diagnosis and to determine the strain of Brucella, then a vaginal swab sample should be taken to perform the PCR test.

Yersinia enterocolitica is one of the most important pathogens transmitted through the digestive tract of pets (dogs and cats) to human. This bacterium is the causative agent of enterocolitis in human. The present study was performed on one hundred dogs (fifty clinically healthy and fifty affected to diarrhea) referred to Veterinary Hospital of Shahid Chamran University of Ahvaz. All characteristics of dogs such as age, gender, breed, gastrointestinal status for diarrhea and characteristic of diarrhea (heamorrhagic), history of raw meat consumption and dog origin were recorded. Then, two stool specimens were taken using sterile glove and swab of the rectum area. The samples were also examined for direct culture and enriched culture (in the buffered peptone water at temperature of 4° C). In cases that the grown colonies were suspected to Yersinia, initial tests (catalase, oxidase and gram staining) were performed and identified with biochemical tests. The probable isolates of Yersinia enterocolitica were recognized by PCR technique and then foxA gene was used to identify bacterium and determine the virulence with ail gene (pathogenicity). Three suspected isolates from one hundred samples (one case of healthy dogs and two cases of diarrheic animals) were included in the results of direct culture. Results of enriched culture were composed of six suspected isolates from one hundred cases (three out of healthy dogs and three other cases of diarrheic animals). Finally, out of the nine suspected isolates to Yersinia, only one sample was confirmed for Yersinia enterocolitica that was negative for ail virulence gene. The positive sample was belonged to a healthy, male and ten-months-old Pit bull terrier imported dog that was fed with raw meat. The results of this study showed that the dogs of this area cannot be considered as a potential reservoir for transmission of Yersinia enterocolitica. Further studies are necessary to determine the status of the disease, with more emphasis on the imported and rural dogs.

The purpose of this study was to identify and compare echocardiographic, radiographic and blood findings of dogs with uremic pericardial effusion. The study was conducted on 6 terrier dog. After examination, the dogs were equally divided into two groups: patient (uremic pericardial effusion) and control (without any cardiac disorders). All dogs were recorded to evaluate echocardiography, radiography, kidney biochemistry and blood factors. The results of radiography showed that the shadow of the heart of all patient dogs is round and large. There is a statistically significant difference between the echocardiographic factors measured in the two factors of ejection fraction and the pericardial fluid between groups (Respectively P=0.05 and 0.001). The average hematocrit, hemoglobin concentration and MCV in the patient group were significantly lower than the control group (Respectively P=0.04, 0.01 and 0.01). Also, the Mean of Urea and BUN in the patient group was significantly higher than the control group (P=0.02). In addition, the findings revealed that there is a positive and significant statistical correlation between the amount of fluid accumulation in the pericardium and Urea and BUN (P=0.04). Therefore, it can be concluded from the results of this study that, firstly, echocardiography is a suitable tool for diagnosing pericardial effusion disease, and secondly, in the severe state of changes in renal enzymes, the amount of fluid accumulation in the pleural membrane is higher.

Pheochromocytomas are tumors of the adrenal medulla that affect chromaffin cells. These tumors are not common in dogs but may occur in older dogs with no specific breed noted. They can be solitary or bilateral, functionally active or inactive, benign or malignant. They are malignant if have long-distance metastasis to other organs such as spleen, lung, regional lymph nodes, bone, and CNS. A 9 -9-year-old spayed female Shih Tzu dog was present at Small Animal Hospital with a history of generalized weakness, anorexia, and depression. In ultrasonography, unilateral enlargement of the left adrenal gland was seen and by surgery, the left adrenal gland was removed.Cytologic evaluation by Giemsa staining showed neoplastic polygonal cells with anisokaryosis.
Histopathologic study on tissue specimen revealed a large mass composed of polygonal, uniform vacuolated cells with granular red cytoplasm. Pigmented granules containing hemosiderin and melanin were present in neoplastic cells.
According to diagnostic ultrasonographic and pathologic findings, this tumor was diagnosed as pheochromocytoma. This clinical report aims to report the diagnosis and successful treatment of pheochromocytoma tumor by surgical method in a 9-year-old Shih Tzu dog and to describe the pathological features of this rare mass.

The bacterium Capnocytophaga canimorsus is a relatively newly recognized gram-negative, facultative, slow-growing bacillus that forms part of the normal oral flora of dogs and cats. Considering the pathogenicity of this bacterium in humans, determining its prevalence is very important for public health as well as the health of dog owners.

This study aims to investigate the prevalence of Capnocytophaga canimorsus in the normal oral flora of healthy dogs.

After taking samples from the saliva of 32 healthy dogs without oral, dental or digestive diseases at different ages, breeds, and sexes, they were placed in a test tube containing 10 mL of sterile peptone water with sterile plastic brushes, and immediately sent to the bacteriology laboratory under sterile conditions. The samples were cultured on a chocolate agar medium containing 5 % defibrinated sheep blood. Then, all the samples were kept in a greenhouse for 48 hours at a temperature of 37 °C and under anaerobic conditions. Using a loop, the grown pink colonies were isolated and to confirm the identification of the isolates, polymerase chain reaction (PCR) test was used in three main steps: Gene extraction, PCR reaction, and electrophoresis.

Out of 32 saliva samples, four positive cases of Capnocytophaga canimorsus bacteria were identified by PCR diagnostic method.

Given that Capnocytophaga canimorsus bacterium is present in the oral flora of healthy ​​dogs, dog owners should have sufficient and favorable knowledge about this bacterium and related diseases. The PCR method can be used to detect this bacterium.

Toxocara canis is a zoonotic disease that commonly infects canids. Mammals and birds are sometimes infected with this disease as paratenic hosts. It can also cause accidental infection in humans. The increase in the number of stray dogs, the expansion of urban gardens, and the proximity of dogs to humans increase the risk of human infection with Toxocara canis.

This study aims to determine the prevalence of Toxocara canis infection in dogs and foxes in Zanjan province, Iran.

A total of 484 fecal samples of stray dogs (n=355), rescue dogs (n=49), guard dogs (n=50), and foxes (n=30) in Zanjan were randomly collected from June 2021 to February 2022. The microscopic examination was done following formalin-ethyl acetate sedimentation procedures. Finally, the PCR method was used to confirm the presence of Toxocara canis in positive samples.

Microscopic study revealed that, out of 484 samples, 21 (4.3%) were positive for Toxocara/ Toxascaris eggs. Between these positive samples of dogs and foxes, only 6 samples from dog feces were confirmed as a Toxocara canis infection by the PCR method.

There is an increase in the prevalence of Toxocara canis infection in stray dogs in Zanjan, Iran. Given the presence of dogs in parks and residential areas, there is a risk of human infection with Toxocara canis, emphasizing the importance of adhering to treatment and prevention protocols in dealing with stray dogs.

Babesiosis is classified as blood parasitic disease that is caused by genus Babesia. This parasite is transmitted by hard ticks and blood transfusion and causes fever, anemia, hemoglobinuria and jaundice. Study on babesiosis is important in terms of its public health concerns. This study was performed to evaluate serum changes of thyroid function indices [Anti-Thyroglobulin (a-Tg), Anti–Thyroid Peroxidase (a-Tpo), Thyroglobulin (Tg), Triiodothyronine (T3), Tetraiodothyronine (T4)], Cystatin C and sphingosine 1-phosphate in dogs with babesiosis. For this purpose, 5 ml of blood were taken from the cephalic vein of 35 dogs with babesiosis in several small animal clinics (in Tehran, Tabriz and Mashhad) which were diagnosed based on clinical signs and laboratory tests and the same number of healthy dogs (35) with consent of their owners and after serum preparation, thyroid function parameters (a-Tg, a-Tpo, Tg, T3, T4) Cystatin C and sphingosine 1 phosphate were measured by specific ELISA kits and evaluated statistically with SPSS version 17. The results indicated significant increase (p≤0.01) in all of the measured parameters in comparison with the healthy group (control group). Based on the ROC statistical test, the obtained results indicate that due to the high sensitivity of the method used in measuring the indicators of thyroid function (except tetraiodo-thyronine) and cystatin C, it is possible to use the serum values of the mentioned indicators (Tg, T3, a-Tg, a-Tpo) and cystatin C as possible potential biomarkers for the diagnosis of canine babesiosis.

Temporomandibular joint (TMJ) is created by the connection between the mandibular condylar process and the mandibular fossa of the temporal bone in all mammalian species. This is a synovial joint and has a fibrocartilage disc. The joint disorders affect mastication, movements of TMJ, and mandibular functions. TMJ imaging can be evaluated by radiography; however, CT imaging is preferred, since the images have no superimposition and present more details of different structures and therefore show a more accurate evaluation of TMJ.

This study aimed to provide accurate statistics on TMJ disorders and to evaluate their prevalence based on CT-scan findings in dogs and cats.

In this study, 265 cases of cats and dogs (102 cats and 163 dogs) were evaluated. A total of 59 cases (38 cats and 21 dogs) had temporomandibular joint disorders. In this study, information on sex, breed, and age regarding TMJ disorders were described in detail.

In the evaluated CT images, osteoarthritis, fracture, dislocation (luxation and subluxation), ankylosis, osteochondrosis, dysplasia, and osteomyelitis were observed.

This study indicated that although the total number of dogs was larger than that of cats, the incidence of temporomandibular joint disorders was higher in cats than in dogs. The most common disorders in cats and dogs were fractures, followed by dislocations (luxation and subluxation), and then osteoarthritis exhibited the highest frequency. In the next rank of frequency in cats, ankylosis and then osteomyelitis were observed. Additionally, osteochondrosis and dysplasia and then ankylosis were observed in dogs in the next ranks.