جستجوی مقالات مرتبط با کلیدواژه « aloe vera » در نشریات گروه « دامپزشکی »

Despite the medicinal properties of Aloe vera, several active compounds found in the plant raise concerns about its safe consumption and application, particularly concerning the male reproductive system. Previous studies on the impacts of Aloe vera on male reproductive hormones were limited to testosterone and gonadotropins, with conflicting results. To date, no research has been conducted on the effect of Aloe vera on the hormones, such as inhibin B and anti-mullerian, which could shed light on the plant's effects on the male reproductive system. The aim of this experiment was to evaluate the effect of hydro-alcoholic Aloe vera gel extract on serum levels of hormones including inhibin B, gonadotropins, testosterone, and anti-mullerian hormone. For this purpose, 18 adult male rats were divided randomly into three groups (one control group and two treatment groups). The rats in the first and second experimental groups received the Aloe vera hydro-alcoholic extract at doses of 100 and 200 mg/kg, respectively, for 60 days. In the end, the levels of reproductive hormones were measured in the serum samples by the ELISA method. Treatment of rats with two doses of hydro-alcoholic Aloe vera extract (100 and 200 mg/kg) resulted in a non-significant decrease in the levels of testosterone and LH and a non-significant increase in the levels of FSH, inhibin B, and anti-mullerian hormones in adult male rats. Therefore, it was concluded that long-term consumption of hydroalcoholic extract of aloe vera gel has no significant effect on the level of pituitary-gonadal hormones in adult male rats.

Exposure to nicotine and cigarette smoke has dangerous effects on pregnant women, fetal and neonatal health, and fertility rates. Aloe vera is one of the few medicinal plants that has maintained its popularity for a long time due to its many medicinal properties. Therefore, in the present study, the protective effect of aloe vera gel on ovarian histological changes in nicotine-receiving rats was investigated. For this purpose, 32 female Wistar rats into 4 control groups (normal diet), nicotine (6 weeks nicotine at 0.2 mg / kg body weight of animals injected intra peritoneal), nicotine-aloe vera (6 weeks nicotine injection + gel) Aloe vera plant (400 mg / kg body weight of animals orally), Aloe vera group (6 weeks aloe vera gel by the above method and amount) were divided. At the end of the course, the mice were facilitated and the ovaries were transferred to a histological laboratory to prepare a tissue slide and perform hematoxylin-eosin staining. The results showed that after nicotine treatment, weight loss, ovarian weight, number of healthy and active follicles, new corpus luteum and increase in the number of atretic follicles were observed. But the use of aloe vera gel was able to improve all the microscopic complications and disorders and macroscopic changes caused by nicotine injection. Therefore, oral consumption of aloe vera gel can reduce or inhibit the harmful effects of nicotine in tobacco or drugs on the ovaries and fertility in women.

Staphylococcus aureus is an important cause of wound infection and found on the skin of normal human and animal population. Hypericum perforatum and Aloe vera have antioxidant, anti-inflammatery and antibacterial effects, so they can affect on wounds positively together. For this purpose, 40 rats were selected and randomly divided into 4 groups: negative and positive control groups and two groups that treated with Hypericum perforatum and Aloe vera ointments that containing 1 and 4 percent extracts. The wounds were pictured on 0, 3,7 and 14 days after wound creation and the wound healing rates was calculated clinically. Histopathologic stadies were performed on samples collecteal from the wounds in 3,7 and 14 days after wound creation. Finally, the data were analyzed by Anova, Kruskal wallis test. The percentage of wound healing in 3th day in two groups treated with ointment containing extract was higher than other groups. on the 14th day the clinically epithelial tissue of the two groups treated with plant extracts was better than the other two groups. Appendices of the skin like hair follicles are formed in two groups contains extracts, but in the extract of %4 was observed more better than the other three groups becase the sebaceous glands were just observed in this group and quality of epithelial tissue was more better. The results show that ointment containing %4 Aloe vera and Hypericum perforatum extracts have better healing effects and skin beauty results than %1 ointment on wounds that inoculated with Staphylococcus aureus.

Recently, studies have been conducted to use plant compounds as natural preservatives in foods. So, the objective of this study was first to evaluate antimicrobial activity of ethanolic and methanolic extracts of Aloe vera, German chamomile and Mentha piperita L cultivated in Medicinal plants farm of agricultural research institute of zabol university, against spoilage and pathogenic microorganisms associated with pasteurized milk including Pseudomonas aeruginosa, Bacillus cereus, Micrococcus luteus, listeria monocytogenes and Escherichia coli and the next stage was to investigate the impact of ethanolic extracts of aloe vera and German chamomile on the shelf life of pasteurized milk. The results indicated that Aloe vera had the most antimicrobial activity followed by German chamomile and Mentha piperita L respectively (P<0.05). In general, the ethanolic extract of studied plants was found to posses more powerful antibacterial activity than methanolic one (P<0.05). Ethanolic extracts of Aloe vera and German chamomile were evaluated as natural preservatives at concentrations of 0.15, 0.3 and 0.6 (% v/v). The results revealed that the treatments of pasteurized milk with 0.3% and 0.15% of German chamomile and also Aloe vera with a concentration of 0.3% with acceptable sensory properties had a significantly lower total microbial count and longer shelf life compared to the control sample. Therefore, this study confirmed the possibility of using of the extract of mentioned plants as a preservative in pasteurized milk besides its beneficial properties of a functional food.

Recently, studies have been conducted to use plant compounds as natural preservatives in foods. So, the objective of this study was first to evaluate antimicrobial activity of ethanolic and methanolic extracts of Aloe vera, German chamomile and Mentha piperita L cultivated in Medicinal plants farm of agricultural research institute of zabol university, against spoilage and pathogenic microorganisms associated with pasteurized milk including Pseudomonas aeruginosa, Bacillus cereus, Micrococcus luteus, listeria monocytogenes and Escherichia coli and the next stage was to investigate the impact of ethanolic extracts of aloe vera and German chamomile on the shelf life of pasteurized milk. The results indicated that Aloe vera had the most antimicrobial activity followed by German chamomile and Mentha piperita L respectively (P<0.05). In general, the ethanolic extract of studied plants was found to posses more powerful antibacterial activity than methanolic one (P<0.05). Ethanolic extracts of Aloe vera and German chamomile were evaluated as natural preservatives at concentrations of 0.15, 0.3 and 0.6 (% v/v). The results revealed that the treatments of pasteurized milk with 0.3% and 0.15% of German chamomile and also Aloe vera with a concentration of 0.3% with acceptable sensory properties had a significantly lower total microbial count and longer shelf life compared to the control sample. Therefore, this study confirmed the possibility of usingof the extract of mentioned plants as a preservative in pasteurized milk besides its beneficial properties of a functional food.

The aim of the present study was to evaluate the antimicrobial effects of aqueous, ethanol, methanol, acetone, chloroform and ethyl acetate extracts of Aloe vera leaves on two fish infectious bacteria, Vibrio cholerae and Listeria monocytogenes. The Aloe vera extracts obtained through rotary apparatus and V. cholerae and L. monocytogenes provided from Iranian fungi and bacterial collection. Minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of the extracts were determined using dilution in well method. Moreover, the diameters of inhibition zones was determined through disk diffusion method. Results of this study showed while aqueous extract of Aloe vera had inhibitory and lethal effects on L. monocytogenes, but have no influence on V. cholerae. The most inhibitory and lethal characteristics of studied extracts on both bacteria was due to chloroform solvent, while had more influence on L. monocytogenes. Chloroform extract tend its inhibitory property even in the most diluted form. Like inhibitory properties, the biggest diameters of inhibition zones was from chloroform extract against L. monocytogenes. Acording to edibility of Aloe vera, its availability and reasonable value, it is possible to use Aloe vera as an alternative for conventional antibiotics and chemical disinfectants in aquaculture rearing pools.

Article 6, Volume 13, Issue 2 - Issue Serial Number 55, Summer 2017, Page 48-57 XML PDF (477 K)
Document Type: Research Paper
DOI: 10.22055/ivj.2017.28887.1550
Abstract
Aloe vera has many applications in traditional medicine .In present study, the hypoglycemic effects of the Aloe vera and its protective effects on the adrenal gland on diabetic rats were studied. 50 mature male rats (2-3 months old) and weighing 200-250 were divided randomly into 5 groups (10 rats in each groups) as following: Group I (control), has not received any treatment. Group II (Diabetic) which received 65 mg/kg Streptozotocin (IP). and rats with blood glucose more than 250 mg/dL were considered as diabetic. Group III, diabetic rats which received the Aloe vera gel with dose of 400 mg/kg daily. Group IV, diabetic rats which received insulin in 10 units/rat. Group V,received Aloe vera gel only. On days 15 and 30 of experiment, blood glucose of all groups were measured. Animals were euthanized with ether. Then, tissues samples were collected from adrenal glands, and fixed in 10% neutral buffered formalin solution. The sections were made using routine paraffin embedding method and stained by haematoxylin-eosin (H & E). Histomorphometrical parameters were evaluated using Digital Dino-Lite lens and Dino capture software 1. The results showed that thickness of fasciculata layer and the size of the spongiocyte were increased significantly in diabetic rat (P≤0. 05) but the thickness of fasciculata layer was decreased significantly in diabetic rats which received Aloe vera and insulin (P≤0. 05). The blood glucose was decreased significantly in diabetic rats. Conclusion, Aloe vera can be has beneficial and protective effects on adrenal glands in diabetic rats.

Diabetes is a metabolic disease that is associated with hyperglycemia and infertility. Previous studies indicate that aloe vera may positively affect the blood glucose and fertility.
the present study was carried out to evaluate effect of aloe vera on serum oxidant/antioxidant activity and reproductive hormones following experimental diabetes.
sixty adults male wistar rats were divided to 5 groups. control group(A) was kept without treatment. Group(E) only received aloe vera gel(400 mg/kg-orally). Experimental diabete mellitus was induced in 3 groups of rats by streptozotocin( 65 mg/kg-Ip). One diabetic group was kept without treatment(B). Another diabetic group received aloe vera gel(400 mg/kg-orally)(C) and another received insulin(10 units)(D). Aloe vera gel and insulin was administration for 30 days then, the rats were anesthetized and blood collected. The amount of malondialdehid(MDA), anti oxidant activity(AOA), glutathion peroxidase activity(GPX), thiol protein(TSH), testosterone, LH and FSH was determined in serum.
level of testosterone was significantly decreased while amount of MDA, TSH, GPX and AOA was significantly increased in non-treated diabetic rats. Aloe vera increased antioxidant defense.
Aloe vera improves antioxidant activity and reduces diabetic complications.

Despite the increasing use of Aloe vera in aquaculture, toxicity and possible side effects have not been studied in rainbow trout. The aim of this study was to investigate the possibility of some tissue change in farmed rainbow trout (Oncorhynchusmykiss) fed with feed containing 1% of hydro-alcoholic extract of Aloe vera. Six hundred pieces’ healthy fish, average means 13 grams were randomly allocate into 2 groups, including Aloe vera extract-treated (1%) and control (placebo 1%), each of triplicates. Their feed has been previously prepared. At the end of the tenth week, 2 fish pieces was caught in each replicate. After anesthesia, the fish were autopsied. The liver, intestine, kidney and gills of fishes were removed. Specimens were fixed in 10% formalin, stained by using Hematoxylin and Eosin (H&E). Finally, prepared tissues were evaluated by optical microscopy. The results were no shown any changes in intestinal histology in the Aloe vera extract-treated group. However, melanomacrophage centers development was observed in liver and kidney in some fish's Aloe vera extract-treated. Such limited tissue changes were natural reaction of studied tissues to the foreign matter (xenobiotics). Some of the gill specimens in both groups showed epithelial cells hyperplasia in primary gill lamella, which is no related to medicinal treatment. It was possibility concern to environmental conditions and water quality. Based on the above findings, it seems that the selected dose of Aloe vera extract has not been detrimental to vital organs of fish in this research study.

Although the Aloe vera plant is well known for its anti-inflammatory, anti-viral, anti-bacterial and immunostimulant properties, but it is more known because this herb has a role in wound healing and freshness in human skin. However, Aloe­ vera has not been used for wound healing in aquaculture. This study was carried out to evaluate the healing effect of Aloe vera on the surgical wounds in fish skin. For this purpose, 150 common carps (120-150 g) were divided into following 5 groups. Fish were anesthetized with MS222 (Tricaine methane sulfonate) and then skin wounds with 5mm diameter and full thickness and circular shape were created by surgical punch biopsy on the left side of each fish. In order to study the healing effect of Aloe vera extract following treatments were applied: group 1 received no treatment (control group), group 2 received a permanent floating in 3% Aloe vera extract, group 3 received oral treatment, through diet containing 5% Aloe vera extract, group 4 received a short time bath containing 10% Aloe vera extract (10±1 min) and group 5 received a permanent floating in 0.5 mg/lit malachite green. Tissue samples were taken from the skin wounds at day 7th, 14th and 21st from six fish in each time, fixed in 10% formalin and then processed through routine histological methods.The results showed that epithelialization, regeneration of injured subepidermal compartment, formation of granulation tissue, angiogenesis and formation of scales were better in group 4 than the other groups. In day 21st, formation of normal epidermis, dermis and scale were more completed in group 4 compared to the others. On the basis of these results, it can be concluded that short time bath with Aloe vera has positive effects on the wound healing in fish. However, permanent floating of fish in Aloe vera may alter water quality and food intake and indirectly has negative effects on wound healing. In order to obtain a more solid results further research is recommended.

Retina is a part of the central nervous system derived from the neuroectoderm and made up of 5 layers. In this study، the changes in the thickness of the retinal layers as a consequence of diabetes and effect of Aloe Vera gel extract in male rats were assessed. Thirty Sprague Dawley adult male rats (175 ± 25 g) in two age groups (4 and 8 weeks) were divided into 6 groups of 5 as control، diabetic and diabetic receiving 400 mg/kg Aloe Vera extract. Diabetes was induced by IP injection of 50 mg/kg of STZ (streptozotocin). The animals were weighed and their blood sugar was measured by glucometer before STZ administration and 24 h thereafter. Animals were anesthetized with sodium thiopental (40 mg/kg) via IP injection. After removal of eyes on both sides، retina was dissected out precisely and fixed in 4% glutaraldehyde، post fixed in osmium tetroxid 1%، dehydrated and then embedded in TAAB resin. Thin sections (1 μm) were stained with toluidine blue stain and viewed under light microscope. Ten slides were prepared from each animal. The results revealed reduction of the blood glucose levels and body weight in treated rats in comparison to diabetic groups. The thickness of neural retina and its layers were different as well. In the group treated with Aloe Vera the thickness of retina and its layers retained their normal histologic structures.