Physico-chemical properties and characterization of chitosan extracted from Penaeus monodon wastes via two different conventional extraction method

The aim of present study was to evaluate the effect of different extraction methods on physicochemical properties and characterization of chitosan extracted from waste of black tiger shrimp (P. monodon). For this purpose, the extracted chitin from by-products of shrimp processing plant was deacetylated to chitosan, which was soluble in dilute acid. Structural and physicochemical properties of chitosan were evaluated, with FTIR (Fourier infrared spectroscopy) and XRD (X-ray diffraction), and in addition the amount of ash, solubility and degree of deacetylation were evaluated. Crystallization index (%) of chitosan obtained by two different conventional methods was ~ 34% and 36%, respectively. The results showed that the degree of chitosan deacetylation obtained by conventional methods (Hydrocholoric acid and acetic acid) were 94% and 89%, respectively, which did not show much difference. The presence of amine groups in the synthesized chitosan was confirmed by the FTIR spectrum.