Effect of eight weeks of forced physical activity with royal jelly consumption on depression and anxiety levels and antioxidant capacity in trimethyltin-induced Alzheimer's rats

The aim of this study was to investigate the protective role of Royal Jelly as an antioxidant on behavioral disorders of depression and anxiety caused by trimethyltytin toxin and the level of superoxide dismutase and glutathione peroxidase enzymes in Alzheimer's rats treated with trimethyltin after eight weeks of forced swimming exercise.

The present study was performed experimentally with 48 male rats; 40 of them were induced by Alzheimer's TMT. The rats were randomly divided into six groups: Healthy control, Alzheimer's control, Alzheimer- forced exercise, Alzheimer- sham, Alzheimer- forced exercise- royal jelly and Alzheimer's-Royal Jelly were divided. Forced Swimming Test (FST) for depression assessment and Elevated Plus-Maze (EPM) for anxiety assessment were used. The expression of SOD and GPX genes were evaluated by Real Time PCR technique. The 2-ΔΔCT formula was used to quantify the expression values of the target genes. Due to the abnormal distribution of some data, the non-parametric statistical method of Kruskal–Wallis was used by SPSS version 20.

Significant differences in groups with dependent variables (p <0.05) showed that swimming training with Royal Jelly reduced depression and anxiety, and swimming training did not have a significant effect on SOD and GPX. But taking Royal Jelly alone increased the SOD and GPX index.

Trimethylthine administration induced depressive and anxiety-like behaviors in rats. This volume of forced swimming training protocol for Alzheimer's rats does not seem to have a positive effect on antioxidant parameters. However, the positive effect of royal jelly consumption on increasing SOD, GPX was observed, and the use of royal jelly with antioxidant properties and the combination of forced exercise with the use of royal jelly supplement has the ability to modulate mood in the current model, and can be used as a useful method.