Investigation the Relationship Between bla OXA Genes and Antibiotic Resistance of Acinetobacter Strains Isolated from Treatment Centers of Alborz Province in 2017

Due to the spread of infections caused by broad-spectrum beta-lactamase-producing actinobacteria in recent years in our country, this study was conducted to determine the pattern of antibiotic resistance and to determine the prevalence of blaoxa23 and blaoxa10 genes in Acinetobacter strains isolated from patients admitted to centers The treatment was taken.

Using specific culture media and biochemical tests, 75 clinical isolates of Acinetobacter were collected from patients hospitalized in Alborz province. 70 isolates were identified as Acinetobacter baumanni by biochemical and molecular methods. Disk diffusion method was used to determine the antibiotic identity of isolates according to the CLSI instruction. In this study, 14 antibiotic disks were used. Finally, polymerase chain reaction (PCR) was used to investigate the prevalence of bla oxa23 and bla oxa10 genes in antibiotic resistance using specific primers. The blaoxa51 gene, which is characterized by Acinetobacter Bumanni, was used to confirm the isolation of the isolates.

  The results showed that 100% resistance to ciprofloxacin, sulfumetoxazole, ceftriaxone, cefotaxime, cefoxitine, imipenem, meropenem and piperacillin - tazobactam antibiotics and the least resistance to colistine (0%), tobramycin (78.5% ), Ampicillin Sulbactam (92.8%), Levofloxacin, cefepime and ceftazidime, each of which was 98.5%. The results of PCR to detect strains containing resistance genes showed that the prevalence of blaoxa23 and blaoxa10 genes was 90% and 61%, respectively. The blaoxa51 gene, which is characterized by Acinetobacter Bumanni, was found in 100% of isolates.

The present study suggests that Acinetobacter Berumani bacteria is highly prevalent in patients admitted to treatment centers of Alborz province. Regarding the identification of blaoxa 23 and bla oxa10 genes in Acinetobacter and relationship its between Antibiotic resistance, it is recommended to revise the pattern of antibiotic use and the criteria for controlling infectious diseases.