جستجوی مقالات مرتبط با کلیدواژه « سلول‮های بنیادی مزانشیمی » در نشریات گروه « پزشکی »

Due to the increasing importance of repairing and rejuvenating different tissues, it is necessary to pay attention to the development of treatment methods to improve treatment results. Low-level lasers are currently used to repair skin tissues. The carboxytherapy method has become particularly important due to its non-invasive treatment. In this method, some carbon dioxide is injected into the tissue.
It is produced naturally by the cells of the body, but when the amount of CO2 becomes too much, it forces the body to respond. When carbon dioxide enters the body, the circulatory system in the treated area is stimulated. And then tries to control the condition using blood vessels. Due to the clinical effects of low-level laser and also the carboxytherapy method, it is necessary to study carboxy effects and simultaneously the low-level laser at the cellular level.
In this study, the proliferation and viability of Adipose Mesenchymal Stem cells (MSC) have been studied under Laser and carboxy therapy treatments to help develop more effective protocols.

Adipose Mesenchymal Stem cells were cultured in DMEM F12 + 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 mg/ml streptomycin. The cells were placed in an incubator with 5% CO2 humidified atmosphere at 37°C. MSC stem cells were irradiated by low-level laser with various doses of 2,3,5 and 7 J/cm2 and also cells were exposed to carboxy with different concentrations (with speeds and Different volumes) by Carboxy Therapy device. viability of cells were done by MTT test.

The results of this study illustrated the viability and proliferation of cells under the laser and carboxy therapy increased and at the simultaneous laser and carboxy treatment showed further increase than the control group.

Based on the findings of this study, the use of carboxytherapy as well as the simultaneous use of laser with an optimal dose can be used as an alternative method in skin repair.

Women of reproductive age may encounter challenges related to infertility or miscarriage, due to conditions such as premature ovarian failure (POF). POF, affecting approximately one percent of women under the age of 40, can lead to infertility. Current data have suggested that utilizing stem cell therapy was the most effective approach for treating POF compared to alternative options. Among the various stem cell types, mesenchymal stem cells derived from the umbilical cord (HUC-MSCs) was a promising choice for treatment due to its features such as relatively low immunogenicity, multipotent, multiple origins, cost-effectiveness, ease of production, and high efficiency.

One of the ways to treat POF is the use of mesenchymal stem cells derived from the umbilical cord, these cells have an extraordinary capacity for repair and regeneration, which helps them in repairing depleted ovaries. The results of this review article indicate that the use of HUC-MSCs can be considered as a potential treatment method in these patients.

The Mesenchymal Stem Cells (MSCs) are a population of widely used cells in cell/gene therapy which are necessary to maintain their stemness properties to guarantee their proper function in medical applications. Platinum nanoparticles are one of the materials that have various applications in the medical world. The aim of this work was to evaluate platinum nanoparticles effect on MSCs cell cycle and Oct-4 and Sox-2 expression in vitro.

In this experimental study, after the synthesis of platinum nanoparticles, their dynamic shape and size were checked by TEM and their charge by DLS test. Then, the culture of mesenchymal stem cells and their survival after treatment with platinum nanoparticles (pt) were investigated. In addition, the expression levels of Sox-2 and Oct-4 genes were evaluated in cells treated with nanoparticles. Also, the effects of platinum nanoparticles on cell cycle and colony formation in stem cells were evaluated. Statistical analyzes were performed with Graph Pad Prism version 10 software. The significance level was defined as p<0.05.

Based on the obtained results, the morphology of spherical nanoparticles and their size was 180 nm and their charge was +25.8. Cytotoxicity test showed that the effects of these nanoparticles on cells are dose and time dependent. Also, in the presence of platinum, the cell cycle of stem cells underwent many changes, especially the increase in the percentage of cells in the S phase. Real-time PCR results showed that in the presence of platinum nanoparticles, there is a decrease in Sox-2 and Oct4 gene expression, which has led to a decrease in the stemness properties of mesenchymal stem cells. Also, their clonogenic potential was also weakened.

We concluded that due to the possible negative effects of platinum nanoparticles on the stemness of MSCs, platinum nanoparticles should be used with caution in long-term medical applications. Therefore, it is suggested to reduce the dose, frequency and duration of using platinum nanoparticles as much as possible.

Degenerative joint disease, especially osteoarthritis (OA), is a global disease characterized by the destruction of articular cartilage, and subchondral bone. It is estimated that about 250 million people currently suffer from cartilage defects. So far, no definite and standard treatment method for OA has been reported. Recently, cell-based therapeutic techniques have been considered one of the best therapeutic strategies for the long-term treatment of articular cartilage diseases. However, many challenges include the large scale of cells required, thus the cell-free approaches are novel tools for cartilage defect treatment. For instance, extracellular vesicles (EVs) secreted by various cells such as MSCs are in charge of the therapeutic effects of stem cells. Therefore, recently EVs have advanced as powerful cell-free transfer tools, due to their high physicochemical strength and biocompatibility.

This study is a review study that summarizes the preclinical and clinical studies that used EV-derived from different cell sources and investigates their effectiveness in treating cartilaginous tissue lesions. Current studies used small or large animal models with experimental critical size defects in knee articular cartilage to examine the effectiveness of EVs derived from MSCs. The EVs were isolated from cell sources such as adipose-derived MSCs, Bone marrow-derived MSCs, or transgenic cells. In addition, EV isolation techniques as a main challenge in studies using EVs to treat OA, specifically described in the current study. We also showed EVs isolated from each cell have unique features such as anti-inflammatory, differentiation, and therapeutic properties. We explain recent studies that use EVs as a drug carrier such as small molecules, and microRNA bioactive factors. In addition, the isolation techniques of EVs and their characterization are other challenges that we explain.

Recent studies have shown that EVs isolated from different sources inhibit the progression of OA. Also, the results of some studies indicate the ability of EVs to repair injured cartilage. Many studies showed that in critical size defects of cartilage, the use of EVs needs scaffolds. Several studies have investigated the challenges of EV release and the required EV dose based on the size of the lesion. EVs are rapidly emerging as novel therapeutic approaches for treating cartilage lesions and OA. Despite many advances in cell therapy and promising results reported in numerous disease models, the use of cells especially genetically modified cells has limitations in regenerative medicine. It is worth noting that the use of EVs derived from stem cells or transgenic cells has no harm to the human body. As a result, therapeutic EVs have been introduced as a new therapeutic approach that does not have the same potential risks as cells.

Despite the positive results of EV in the treatment of cartilaginous lesions, it appears that the EV therapeutic barrier requires further testing in larger animal models before clinical trials. For instance, the regeneration of critical-size lesions requires the use of EVs incorporated by suitable scaffolds under dynamic conditions. Therefore, the fundamental questions to be considered are: How to use EVs as a nanoparticle instead of stem cells in combination with tissue engineering methods? What are the biological properties of EVs? What doses of EVs have the mechanistic potential for the treatment of different sizes of cartilage lesions and how EVs are stable in lesions? What is the role of EVs in the homeostasis and pathogenesis of junctions?

7SK is a long non-coding RNA that interacts with various proteins to regulate gene transcription. This study aimed to assess the impact of exosomal delivery of 7SK on viability, expression of apoptosis-related genes, and migration in the human colorectal cancer cell line HT-29.

In this experimental study, HT-29 cells were treated with exosomes derived from human umbilical cord mesenchymal stem cells loaded with 7SK (Exo-7SK). Control groups included cells treated with unloaded exosomes and untreated cells. The levels of 7SK in the cells, and expression of apoptosis-related genes were measured by real-time PCR, cell viability assessed by the MTT assay, and cell migration evaluated using the transwell assay.

Treatment of HT-29 cells with Exo-7SK resulted in increased levels of 7SK, reduced viability, downregulation of the anti-apoptotic gene BCL-2, upregulation of the apoptosis-inducing gene BAX, and decreased migration.

Exosomal delivery of 7SK can effectively decrease the viability and migration of colorectal cancer cells. Further research is warranted to explore the therapeutic potential of this approach in colorectal cancer.

With the emergence of SARS CoV-2, we have witnessed numerous deaths and injuries. Due to the possibility of emergence of new strains, there is a need to improve treatment approaches. There are reports on the application of placenta-derived mesenchymal stem cells (p-MSCs) as a therapeutic approach. In this study, we investigated the therapeutic effects of these cells in patients with COVID-19.

Nine patients with severe and critical COVID-19 and severe inflammatory condition admitted to the ICU were candidates for cell therapy. For each subject, three 1 x 106 injections of p-MSC (cell/kg) were administered intravenously. The clinical manifestations of the patients and the occurrence of potential early complications as well as the levels of blood and inflammatory and anti-inflammatory parameters before and after cell therapy were investigated.

All treated patients tolerated the p-MSC injection and no acute side-effect was reported for the treatment. The PaO2/FiO2 level increased significantly in the patients after cell therapy. The analysis of the blood, inflammatory and anti-inflammatory parameters of the patients before and after cell therapy showed a significant decrease in the level of RBC and CRP, D-dimer, IL-6 and TNF-α and an increase in platelets and IL-10. The changes in WBC, lymphocytes, neutrophils, monocytes, Hb and PT and biochemical parameters in the serum, fibrinogen coagulation, PH, PCO2, Po2, HCO3 and O2 sat (%) were not statistically significant.

Based on the results, three intravenous injections of p-MSCs in severe and acute patients improved their inflammatory condition and clinical symptoms and had a significant effect on their inflammatory and blood parameters including RBC and platelet.

Premature Ovarian Insufficiency (POI) refers to the loss of ovarian function before the age of 40. This condition can be attributed to various factors including X chromosome abnormalities, autoimmune disorders, and chemotherapy drugs. Hormone therapy is a commonly used treatment for POI, but due to side effects and low fertility rates, alternative treatment options are needed. In recent years, stem cell transplantation has emerged as a promising treatment approach, offering hope for improving and restoring ovarian function. Stem cells possess the unique ability of self-renewal and regeneration, making them potentially effective in addressing ovarian failure and subsequent infertility. Different types of stem cells have been investigated for the treatment of POI, including mesenchymal stem cells (MSCs), stem cells from extraembryonic tissues, induced pluripotent stem cells (iPSCs), and ovarian stem cells. This article aims to provide an overview of the causes and treatment options for Premature Ovarian Insufficiency, with a particular focus on stem cell therapy as suggested by previous studies. Corresponding Author: Hossein Kalarestaghy, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.

Background and Objectives The use of stem cells for skin wound healing is still facing serious challenges.This study aims to determine the effectiveness of human adipose tissue-derived stem cells (ADSCs) sprayin histologically improving skin wounds in male diabetic rats.Subjects and Methods In this experimental study, mesenchymal stem cells were isolated from adiposetissue and cultured and characterized by evaluating the expression of specific CD markers. Microbialcontamination in samples was assessed before treatment. Then, 18 male Wistar rats were dividedinto control and treatment groups. Diabetes was induced by a single injection of streptozotocin. Then,wounds with a diameter of 0.8 cm were created in the back of the rats. The ADSCs were sprayed on thewound area in the treatment group. Wound healing was assessed on days 7, 14, and 21 after treatmentusing visual observation and histopathological examination. The data were analyzed using ANOVA.Results Wound closure was accelerated on days 7, 14 and 21 in the treatment group compared to thecontrol group. Histological examination showed that the skin thickness, angiogenesis, and epithelializationwere higher in the treatment group on days 7, 14, and 21 compared to the control group.Conclusion The spray of ADSCs on the wound area can accelerate the healing process and can be usedfor skin wound repair.

The purpose of this study is to evaluate the efficiency of electrospun scaffolds fabricated of poly (Lactic-co-glycolic acid) (PLGA) was coated by laminin, which are used as conduits to axon growth.

48 adult male Wistar rats were used in this study. These rats were divided into 6 groups, including a healthy control group, and in the other five groups, in addition to 10 mm damage to the sciatic nerve, various treatments were performed, including the Poly (L-lactide-co-glycolide) conduit group coated by laminin and filled with culture medium, PLGA conduit group coated by laminin and filled with rat adipose tissue-derived stem cells, PLGA conduit group coated by laminin containing gold nanoparticles and Brain Derived Neurotrophic Factor (BDNF) encapsulated in chitosan, PLGA conduit group coated by laminin containing gold nanoparticles and BDNF encapsulated in chitosan, which filled with rat adipose tissue-derived stem cells, or in the group Sixth, PLGA conduit group coated by laminin containing gold nanoparticles and BDNF encapsulated in chitosan, which filled with rat adipose tissue-derived stem cells suspended in alginate hydrogel. 12 weeks after implantation of conduits to the 10 mm defect of rat sciatic nerve, nerve regeneration and functional recovery were evaluated by behavioral tests and electrophysiology tests.

Three months post-operatively, the results of sensory analyses in all animals showed that sensory reinnervation on their foot returned almost completely, regardless of their experimental group. Also, different degrees of increase in CMAP (Compound Muscle Action Potential) amplitude were observed in all groups. However, their increase was significantly lower than the control group, while the latency in the groups containing nanoparticles and cells suspended in alginate did not show a significant difference compared to the control group.

The findings showed that electrophysiological and sensory tests can be effective methods to evaluate the peripheral nerve regeneration.

Endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) have useful effects in treatment of sepsis, but the effects of co-injection of EPC and MSC for the treatment of sepsis have not yet been investigated. This study investigated the therapeutic effect of MSC+EPC in reducing the systemic inflammatory response in lipopolysaccharide (LPS) induced sepsis in mice.

In this experimental study, mouse bone marrow MSCs and EPCs were isolated and their surface markers were investigated using flow cytometry. Four groups of mice (n= 12 per group) were included in the study. The control group had no treatment, group II received LPS intraperitoneally, group III received PBS buffer after LPS inoculation, and group IV received EPCs+MSCs after LPS inoculation. The serum and tissue levels of IL-1β, TNF-α, IL-6, and IL-10, and the serum levels of CRP and hepatic enzymes were determined by commercial kits. One-way ANOVA and post hoc test were used for comparison between groups.

The surface markers of MSCs and EPCs were confirmed by flow cytometry. The co-injection of MSC+EPC significantly decreased the levels of pro-inflammatory cytokines (P<0.001), increased the concentration of anti-inflammatory cytokines (P<0.001), and increased the survival rate (P<0.01). Mice treated with MSC+EPC represented a significant decrease in liver enzymes (P<0.05), pulmonary edema (P<0.01), and CRP level (P<0.05) compared to the mice with LPS-induced sepsis.

Co-injection of MSC and EPC leads to a reduction of the inflammatory response. This study provides promising results for the treatment of sepsis.

The fetus is the source of many stem cells that have great potential in repairing and regenerating different tissues and can differentiate into various types of cells including osteoblast and adipocyte. However, less attention has been paid to the isolation and differentiation ability of these cells in the culture medium. Therefore, the present study aimed to determine the isolation method, proliferation, and differentiation of fetal mesenchymal stem cells (fMSCs) derived from rat fetuses.

In this experimental study, fMSCs were isolated from 15-day-old rat fetuses using trypsin enzymatic digestion and they were cultured up to 3th passage. Then, the fMSCs were characterized by flow cytometry and differentiated into osteoblast and adipocyte cells. Cell proliferation and expression of osteogenesis- and adipogenesis-related genes were investigated in differentiated cells.

Cell surface markers of fMSCs at passage 3 were positive for CD105 and CD90 and negative for CD45 and CD34. These cells exhibited a fibroblast-like morphology. These cells had calcium deposition in osteogenic differentiation and accumulation of fat droplets in adipogenic differentiation. The osteogenesis-related genes including osteonectin, ALP, BMP-6, and Runx-2, and adipogenesis-related genes such as PPARγ and CREBBP were expressed in osteogenic and adipogenic differentiation cultures, respectively.

According to the findings of the present study, it seems that mesenchymal stem cells isolated from rat fetuses have the potential to proliferate and differentiate into osteoblast or adipogenic lineages.

Cerebral palsy is a movement disorder syndrome in early childhood. Signs and symptoms vary among people and over time but include poor coordination, stiff muscles, and weak muscles. Some affected children can achieve near-normal adult lives with appropriate treatment. In recent years, transplantation of human mesenchymal stem cells (hMSC) has become a promising therapeutic strategy for CP. Every year, a lot of costs are spent on the treatment and management of this disease. The purpose of this study is to investigate the safety and effectiveness of this method on CP.

This article is a systematic review. At first, a search strategy was written and performed in Scopus, PubMed, and Google Scholar databases(The search was conducted from March 14 to March 28, 2021), and the inclusion and exclusion criteria were determined. Study inclusion criteria: review studies, cohort studies, clinical trial studies (randomized and non-randomized), control case, and retrospective , exclusion criteria: non-English articles and studying on animals. After removing duplicate articles, two authors independently reviewed the studies according to the inclusion and exclusion criteria. Disagreements among the authors were resolved through discussion.

In total, 9236 articles were found in the initial search, after reading the titles, of 37 articles, 21 articles were selected in the abstract stage and 18 articles remained in the full-text stage. We finally found 18 articles that showed that using stem cell technology as a scientific method could improve sick patients’ quality of life and movement defects.

According to the available evidence and limited studies, stem cell technology can be safeand cost-effective in improving CP patients, but there is insufficient evidence. On the other hand, there are many studies confirming the effectiveness of these cells in the treatment of movement impairment. In conclusion, stem cells may have a very promising future. Finally, stem cell technology combined with innovative biotechnologies may soon bring promising results to patients.

Knee osteoarthritis is associated with damage to ligaments and skeletal muscles around the knee joint. Since inhibiting of atrophy can help to improve this disease; therefore, this study aimed to determine the effect of mesenchymal stem cell (MSCs), hyaluronic acid (HA) and aerobic exercise on the expression of myonectin and myogenin genes and the production of CD44 protein in the quadriceps muscle tissue of osteoarthritis rats.

Forty-five adult male rats aged 6-8 weeks were placed in 9 groups of 5 including: healthy control, patient control, MSCs, HA, aerobic exercise, MSCs+HA, exercise+HA, exercise+MSCs and exercise+MSCs+HA. Osteoarthritis was induced surgically. Aerobic exercise was done for 8 weeks, 3 sessions a week and at a speed of 16m/min. MSCs were injected at the rate of 1×106cells/kg and HA at a concentration of 10mg/ml. Forty-eight hours after completing the protocol, the quadriceps muscle tissue was isolated and the expression of myonectin and myogenin genes was measured by Real-Time PCR and the expression of CD44 protein was measured by immunohistochemistry method.

The gene expression of myonectin, myogenin, and CD44 protein expression was significantly increased in aerobic exercise, HA and MSCs groups alone (P=0.001) and CD44 in exercise+HA (P=0.024). Also, myogenin in exercise+MSCs group (P=0.037) and myonectin in exercise+MSCs, exercise+HA (P=0.001) and MSCs+HA (P=0.012) groups increased significantly.

Aerobic exercise, HA and MSCs alone and exercise+MSCs synergistically led to the inhibition of muscle atrophy by increasing the activity of myokines; but exercise+HA play a role in muscle building in osteoarthritis rats.

Stem cells and tissue engineering have shown great potential in the treatment of periodontal diseases since their introduction. Mesenchymal stem cells are undifferentiated cells that can differentiate into different tissues. The aim of this study was to evaluate the effectiveness of different types of these cells in the treatment of periodontal defects.

Mesenchymal stem cells exist around different tissues and can be obtained and used from different intraoral or extraoral sources for periodontal tissue regeneration procedures. Among extracellular stem cells, bone marrow stem cells followed by adipose tissue stem cells have shown promising results regarding the ability of these cells to regenerate the periodontium. Among intraoral sources, periodontal ligament mesenchymal stem cells and then dental pulp cells have shown more results and ability compared to other cell types to regenerate periodontal tissue. Periodontal ligament stem cells have also been able to generate sharpey fibers in periodontal regeneration procedures in various studies.

Mesenchymal stem cells have shown promising results for use in periodontal tissue regeneration processes. However, due to the limited clinical and preclinical studies performed so far, more studies are needed for assessing the clinical use of these generative of cells.

Carbon tetrachloride (CCI4) is used as a chemical intermediate in industries. It can be converted into toxic reactive products of trichloromethyl radical under the influence of cytochrome P450 enzymes, and cause tissue damage, including liver damage, through oxidative stress. Liver transplantation is an effective treatment for liver failure but is limited due to the shortage of organ donors.

This study aims to evaluate the effect of human mesenchymal stem cell-conditioned medium (hMSC-CM) on the oxidative stress induced by CCI4 in rats.

Twenty-one adult male Wistar rats matched for weight were randomly allocated into three groups of 7: control, treatment A (receiving CCI4), and treatment B (receiving CCI4+hMSC-CM). A single dose of normal saline was injected into the rats in the control group. Treatment group A and B received intraperitoneal injection of CCI4 along with olive oil with a ratio of 1:1. Twenty-four hours after CCI4 injection, the rats in group B were treated by injection of hMSC-CM for three consecutive days. Forty-eight hours after the injection of hMSC-CM, blood samples were collected from all rats. Oxidative stress markers including total antioxidant capacity (TAC) and malondialdehyde (MDA) were measured and the data were analyzed using one-way analysis of variance in SPSS software, version 25.

There was no statistically significant difference in TAC and MDA levels between different groups (P>0.05). The highest levels of TAC was observed in treatment group B, and the highest level of MDA was observed in treatment group A. 

The hMSC-CM has no significant effect on the oxidative stress in the liver. It is possible to achieve significant results by increasing the duration of treatment.

Fibromyalgia (FM) is a chronic pain syndrome affected by the hippocampal function. Mesenchymal stem cells-conditioned medium (MSC-CMs) has shown antioxidant and neuroprotective potentials in treating various diseases. The present study aimed to investigate the efficacy of MSC-CMs on the thermal treatment of reserpine-induced FM and evaluate the concentration changes of tumor necrosis factor-alpha (TNF-α) in the hippocampus of rats under these conditions.

220 g. Animals received reserpine (1 mg/kg) in the FM groups, and subcutaneous injection of normal saline in other groups, once a day for 3 consecutive days. Then, the rats received MSC-CM (0.3 and 0.5 mg/ml protein content, intraperitoneally) once a week for three weeks. The thermal pain threshold of mice was evaluated using tail flick and hot plate tests at the beginning and end of the experiments. TNF-α concentration in the hippocampus was determined by an ELISA kit at the end of the experimental period.

The thermal pain threshold of rats in the FM group decreased significantly (P<0.01) in both pain tests compared to that of the control group. A significant increase (P<0.01) was observed in the level of TNF-α in the hippocampus of rats in the FM group compared to that of the control group. Treatment with MSC-CMs significantly reduced reserpine-induced pain and hippocampal TNF-a concentration despite the effect of fresh and conditioned medium on control rats.

Treatment with MSC-CMs could control thermal pain in the rat model of reserpine-induced fibromyalgia by controlling the TNF-a level in the hippocampus.

Neurodegenerative diseases lead to the death of neurons and dysfunction of the nervous system. Bone marrow-derived mesenchymal stem cells (BM-MSCs) can be used in the regeneration of neuronal cells. Curcumin is the active ingredient of Curcuma longa. The purpose of this study was to examine neurogenerative induction effects of curcumin on the BM-MSCs.

The MSCs were extracted from rats. The cytotoxic effects of curcumin on the MSCs were evaluated by MTT assay. For the purpose of evaluating the induction of neuron differentiation, BM-MSCs were cultured with pre-induction medium for 24 h and then in inductive medium containing curcumin or BHA and DMSO (positive control). The results were evaluated by examining cell morphology via inverted microscope.

The results of the MTT test showed that curcumin (1, 5 and 10 μM) did not affect the growth rate of MSC cells. The results of induction of neuron differentiation showed that the differentiation of BM-MSCs cells was visible from the second hour under treatment with curcumin. While, during the fourth hour onwards, the BM-MSCs were transformed into spherical neurosphere masses like those that were treated in the positive control group.

Curcumin with a suitable inducing effect on the differentiation of MSC into neural progenitor cells can be considered as a potential agent for further studies to introduce an effective drug in the treatment of neurodegenerative diseases.