جستجوی مقالات مرتبط با کلیدواژه « لکوسیت » در نشریات گروه « پزشکی »

Oral vaccines in infectious diseases, whose agents enter the body through the mucous membrane, will induce the innate immune pathway. The neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) have been documented as biomarkers of Brucella systemic inflammation. The purpose of this study is to investigate the effect of oral administration of the Lactococcus lactis probiotic vaccine carrying Brucella abortus BLS antigen on the ratio of blood leukocytes as biological biomarkers in mice with brucellosis compared to the control group.

60 mice with brucellosis (challenged) and 60 healthy mice (non-challenged) were included in the study. The challenge group was divided into 6 subgroups and 6 types of intervention including 1) L. lactis/pNZ8148-Usp45-BLS, 2) L. lactis-pNZ8148, 3) L. lactis, 4) IRIBA Vac, 5) pNZ8148 and 6) PBS received by gastric gavage in all groups except IRIBA Vac. Serum was used to check hematological parameters. C-reactive protein (CRP) level was measured. The number of neutrophils and lymphocytes was determined using a complete blood cell count. PLR and NLR were calculated.

In this study, the level of CRP in the mice of the challenge group increased significantly compared to the non-challenge group. (P<0.0001) The mice receiving oral vaccine L. lactis/pNZ8148-Usp45-BLS were able to inhibit the infection and reduce the concentration of CRP in They were basic level. Mice with brucellosis have lower levels of NLR and PLR than the control group, and after receiving the recombinant vaccine, an increase in leukocytes was observed (P˂0.0001).

The mice of the challenge group and the non-challenge group showed a statistically significant difference in terms of PLR and NLR as well as CRP. These parameters can be useful markers in evaluating the systemic inflammation of brucellosis.

Being Overweight is a global health problem and is related to important underlying diseases such as cardiovascular disease, malignancy, diabetes, fatty liver, etc. Over the past three decades, obesity has increased globally, especially in low-and middle-income countries. Numerous studies have shown that obesity increases the risk of developing chronic and threatening diseases including diabetes, cardiovascular diseases, blood pressure, and reduces life expectancy. On the other hand, chronic inflammation around fat cells plays an important role in obesity-related diseases. Also, changes in blood parameters are associated with increased body mass and chronic inflammation in obesity, and peripheral blood cells, such as the number of platelets, neutrophils, lymphocytes, and monocytes, are related to the progression of various types of diseases and inflammatory conditions. Therefore, the purpose of this study is to find the relationship between blood count biomarkers and anthropometric indicators in the adult group according to the Tabari cohort data.

Materials and methods:

 In the present study, cross-sectional data from a population-based cohort study called "Tabari Cohort Study" collected from 2014 to 2016 were used. This study is part of a nationwide study called "Epidemiological Prospective Study Group in Iran (Persian Cohort)". In the first phase of the Tabari cohort study, 10,255 participants aged between 35-70 years from urban and rural areas of Sari city were registered. Exclusion criteria in this study were inflammatory diseases, autoimmunity, malignancy, cardiovascular diseases, transplantation, and receiving any type of immunosuppressive drug, and finally 9939 people including 4043 men and 5896 women were included in the study. Age, height, weight, anthropometric measurements (BMI, waist circumference), CBC parameters including leukocytes, platelets, neutrophils, lymphocytes, and monocytes), and platelet distribution width (PDW) were recorded from the participants. By using the histogram drawing method to check whether the variables follow the normal distribution to decide between parametric or non-parametric analysis, T-test and ANOVA statistical tests, and SPSS 21 software, the results were extracted. The significance level in this study was considered to be 0.05 or less.

Waist-to-hip ratio was associated with higher WBC (P<0.001), platelet (P<0.001), and lymphocyte percentage (P<0.001). Higher waist-to-hip ratio was associated with higher WBC (P<0.001), platelet (P<0.001), lymphocyte percentage (P<0.001), and monocyte percentage (P<0.001). Higher BMI values were associated with higher WBC (P<0.001), platelet (P<0.001), lymphocyte (P<0.001) and monocyte (P<0.001) percentages. WBC, platelet, lymphocyte, and monocyte percentage were significantly higher in people who had a higher ratio of waist circumference to height (P<0.001).

The results showed that the relationship between anthropometric indicators and blood findings in favor of inflammatory conditions and obesity can affect indicators related to whole blood count.

Nowadays, standard compression stockings are used to correct or balance thrombosis indicators in patients with deep vein thrombosis (DVT). The purpose of this study was to compare the effect of aerobic exercises combined with elastic pressure of the lower limbs on leukocytes, neutrophils, monocytes and lymphocytes in people with DVT.

30 middle-aged men (51 ± 4 Years) with DVT were randomly divided into three groups: 1) Aerobic training, 2) Aerobic exercise with elastic pressure, and 3) Control group. Aerobic training was performed for 8 weeks of running at an intensity range of 60-70% heart rate reserve. In the group of aerobic training + elastic pressure, elastic socks were used to create elastic pressure during training. Fasting levels of neutrophils, monocytes, lymphocytes and leukocytes were measured on 2 occasions: 24 before and 48 hours after the training period. Analysis of variance test was used to compare between-group changes and paired t-test was used to determine intra-group changes.

The results showed that aerobic + stretching training decreased leukocytes, lymphocytes, neutrophils and monocytes (p = 0.001), but the changes in the aerobic and control groups in none of Factors were not significant.

Using elastic compression stockings during regular aerobic exercises in people with DVT, through reducing and balancing pro-coagulation factors including leukocytes and its types including lymphocytes, monocytes and neutrophils leads to a decrease in the blood coagulation process among people with DVT.

The immune system acts as the body's defense mechanism against invasion, protecting people through a variety of cellular and molecular mechanisms. The aim of this study was to investigate the effect of intense intermittent exercise and fenugreek supplementation on some immune system indices in overweight and obese women.

In this semi-experimental study (RCT), (age range, 25±5 years and BMI, 30±2.5kg/m2), were selected purposefully and Using Power software, they were divided into four groups of exercise + fenugreek supplement(n=12), exercise + placebo(n=12), fenugreek supplement(n=12) and placebo(n=12). The exercise group + fenugreek supplement and exercise + placebo performed the shuttle thigh test at maximum speed for 6 weeks, 3 sessions per week. The fenugreek and placebo supplement group did not participate in any sport program. Blood samples (for assessment of Leukocytes, lymphocytes and immunoglobulins levels) were collected before, immediately after training. Data were analyzed using Shapro wick test, one-way analysis of variance, dependent t-test and Scheffe post hoc at a significant level (p< 0.05).

In this study, IGM, IGA and lymphocyte levels changed meaning and IgG and leukocyte levels increased significantly after 6 weeks of intense intermittent training and fenugreek supplementation (p <0.05).

Based on the results of the current study, High -intensity interval training and fenugreek supplementation affects the levels of immunoglobulins, lymphocytes and leukocytes.

In aged women, elevated levels of some proinflammatory markers are risk factors for the development of diseases. The present study investigated the effect of 12-week walking at two different intensities on inflammatory factors in elderly woman.

Thirty untrained elderly women (60—75 years) randomly divided into three groups. Moderate tempo walking group (MTWG, n=11), Brisk walking group (BWG, n=10), and the control group (CG, n=9). The MTWG, walked at 50—55% maximum heart rate reserve (HRRmax) and the BWG walked at %70-75 HRRmax. Both walking groups trained 12 weeks, 3 sessions per week, starting from 30 to 60 minutes and the CG remained untrained. Maximal oxygen consumption (VO2max), body composition, leukocyte counts (WBC) and plasma levels of Tumor necrosis factor-alpha (TNF-α), were measured before and after the study period. To analyze the data, oneway analysis of variance, Scheffe test and dependent t-test was used (P≤0.05).

VO2max, increased significantly in BWG. Body weights, percent body fat (p =0.000), body mass index (p = 0.003) significantly decreased, and percent muscle (p = 0.000) increased in both exercise groups. Percent visceral fat (p = 0.028) significantly decreased in BWG. Also, BWG showed a significant reduction in WBC counts compared to baseline levels (p = 0.004). There were no significant differences in the plasma levels of TNF-α between the groups.

The results showed that regular walking program can affect body composition in elderly women, however, it may not be effective in reducing systemic inflammatory markers.

Milk thistle has been used for centuries as a herbal medicine for the treatment of different diseases. The aim of this study was to investigate the effects of different doses of short-term milk thistle extract supplementation on the inflammatory markers response in active male induced by one-session aerobic exercise.
For this purpose, Twenty-seven active male with VO2max 45-50 ml.kg-1.min, after completing consent forms, in a quasi-experimental and double-blind design were divided in three homogeneous groups of 9 subjects; supplement (Silymarin: 5 and 10 mg.kg1.day) and placebo (Dextrose: 5 mg.kg-1.day). After 14-day supplementation, all subjects were participated in a single aerobic exercise include; running on the treadmill at the 0% slope for 30 min with the intensity of 70-75% HR reserve. Blood samples were taken at three phases (baseline, after supplementation period and 24 hour after the exercise). Data were analyzed using repeated-measure 3×3 ANOVA, Bonferroni and independent t-test at α≤0.05.
The results showed that a single bout of aerobic exercise causes a significantly increased 24-hour inflammatory indices in all groups (P=0.001). However, in comparison to the placebo group, silymarin ingestion groups cause to attenuate the inflammatory markers response (CRP & PBMC) for placebo, 5 and 10 mg.kg-1.day of silymarin, respectively; P=0.023 and P=0.014) following one-session aerobic exercise.
Based on the present findings, it can be concluded that the short-term of silymarin supplementation in dose-dependent response together with aerobic exercise could reduce the inflammatory markers in active males.

The studies has been shown that insufficient recovery between sessions cause immune suppression. The aim of this study was to investigate the effect of repeated bouts resistance and endurance exercise on blood leukocytes, lymphocytes, neutrophils, monocytes count and plasma cortisol levels in athlete girls. 19 athlete girls (ages, 20- 25 yr), volunteers participated in the study, in two phase and two separate days performed two experimental protocols. At first, subject performed two bouts of resistance exercise (8 exercise with 65% 1RM) at 09:00 and 15:00. After one week rest, subject performed two bouts of endurance exercise (60 min cycling on 65% HR Reserve) at same time. Blood samples were taken before and after each bout and 1 hour after second bout of exercise. Statistical analysis was performed using a Kolmogroph-Smirnoff, independent t-test and ANOVA with repeated measure. Repeated bouts endurance exercise significantly increased blood leukocytes, neutrophils, monocytes count and plasma cortisol levels, but of repeated bouts resistance exercise only significantly increased blood lymphocytes count (P˂0.05). Comparison of two type exercise indicate only significantly difference in blood monocytes count (P˂0.05). This results has been shown that no significantly difference between repeated bouts of resistance and endurance exercise in a day, on immunological variables and cortisol hormone.

Immunohematological reactions to stressful situations have been interested of many investigators. The aim of this study was to investigate the effect of an intense anaerobic workout session on some parameters of athletes. In this analytic descreptive cross-sectional study، 20 professional athletes in volleyball field with mean age 5 ± 25 years were invited purposefully to study. In this study، 30 second Wingate test was used to assess anaerobic power. Samples of peripheral blood before، immediately after and two hours after exercise were collected and levels immunohematological indices were determined. The results of this study showed that immunohematological cells such as leukocytes، lymphocytes، neutrophils، erythrocytes and platelets counts before، after and two hours after practice، have changed significantly (P <0. 01). Results showed that one session of intense anaerobic exercise has significant effect on immunohematological indices of athletes.

Several studies have demonstrated that the levels of inflammatory markers in healthy women are under the influence of menstrual cycle changes. The aim of this study was to compare blood levels of inflammatory markers in women with appendicitis in different phases of menstrual cycle.

In this case-control study, 70 female and 12 male patients with appendicitis, and 61 healthy women were enrolled based on inclusion and exclusion criteria. Inflammatory markers, such as leukocyte count, CRP, ESR, and TNF-α were measured and compared using student t-test and one-way ANOVA based on different phases of menstrual cycle.

There were no significant changes in the ESR, CRP, and TNF-α concentrations and the number of peripheral blood leukocytes in different phases of the menstrual cycle in women with appendicitis. The mean number of leukocytes and CRP concentrations were significantly higher in patients with appendicitis compared with the control group.

The findings show that there are not significant differences in the inflammatory markers in women with appendicitis during the different phases of menstrual cycles. It seems that day to day variation of sexual hormones in the menstrual cycle have led to very different conclusions about the change of inflammatory markers in different phases of menstrual cycle. Therefore, studies investigating inflammatory markers in women with acute appendicitis based on day of menstrual cycle, time of sampling in the day, and severity of appendicitis are suggested.

Stress of vigorous exercise may cause disorders in body immune system and appearance of acute and chronic inflammation. Caffeine supplementation prior to exercise can be effective on body immune response. The purpose of this study was to evaluate the short-term caffeine supplementation on some immune and stress index responses in non-active men after an exhaustive aerobic exercise. Twenty non-active male students were randomly divided into supplementation and placebo groups. Exercise test was an exhaustive treadmill test (Bruce test). In a double-blind study one hour prior to the main test، the supplementation group received caffeine (6 mg/BW) and the control group received placebo at the same time. The serum cortisol، leukocyte and eHsp72 levels were measured before and immediately after the exercise test. Data analysis showed that significant increments in lymphocyte، cortisol and Hsp72 concentrations in placebo group was higher than the supplementation group (P<0. 05). Moreover، the mean alterations in these variables in the placebo group was significantly greater than the supplementation group (P<0. 05). The caffeine supplementation prior to the short-term exhaustive aerobic exercise may have positive effect on the immune system.

Both exercise and unusual environments cause changes in the immune system function. The aim of this study was to compare the effect of exercise on the total numbers of blood Leukocytes and platelets of the athletes in cold, warm and normal temperature conditions. In this clinical trial, ten young male endurance athletes conducted the same exercise (treadmill running) for an hour at the intensity of %60 VO2 max in three normal, cold and warm temperature conditions. The number of Leukocytes, neutrophils, lymphocytes, monocytes and platelets were counted before, immediately and two hours after exercise (during recovery). The collected data were analyzed using the repeated measure and post hoc Bonferroni tests. The total number of leukocytes increased significantly after exercise, in all circumstances (p<0.0001). The number of neutrophils, only in warm temperatures, and lymphocytes, in cold temperatures, didn’t increased significantly (p>0.05). During the rest period (recovery), the number of monocytes and lymphocytes decreased significantly in cold, normal and warm environments (p< 0.05) while decreases in the number of leukocyte, exception with neutrophils, was not significant in the warm environment (p<0.05). Exercise in cold, normal and warm environments caused stimulation and aggregation of immune cells. However, the exercise in warm environment increased the number of blood immune cells and also delayed the immune system in reaching the initial condition during the rest period, after the exercise.

Stress of vigorous exercise may be causes of disorders in body immune system and appearance of acute and chronic inflammation. Caffeine supplementation prior to exercise can be effective on body immune response. The purpose of this study was the evaluation of short-term caffeine supplementation on some immune and stress index responses in non-active men after an exhaustive aerobic exercise. 20 non-active male students randomly divided in supplementation and placebo groups. Exercise test was an exhaustive treadmill test. One hour prior to main test supplementation group consumes caffeine and control group receive placebo at the same time. Blood samples were collected before and immediately after exercise test. After supplying serum; Cortisol, leukocyte and serum eHsp72 concentrations were determined using ELISA method. Paired and independent t student test was used for evaluation of inter and between group differences respectively. Data analysis showed that significant increments in lymphocyte, cortisol and Hsp72 concentrations in placebo group was higher than supp group (P<0.05). Also Mean of variation in these variables in placebo group was significantly greater than supp group (P<0.05). Based on study results caffeine supplementation prior to short-term exhaustive aerobic exercise has positive effect on innate immune system and body defensive apparatus.

Cardiovascular diseases are often the result of vessel occlusion by atherosclerosis and quantitative disturbances such as increased blood cholesterol and other plasma lipid levels, have important role in its pathogenesis. The most important cause of infectious diseases and its progress is insufficiency of immune system and especially insufficient activation of leukocytes. Therefore an increase in the number of leukocytes and augmentation of immune system can lead to prevention of infectious diseases. The aim of this research was to evaluate the effect of oral lemon peel essential oil on the level of blood lipids and differential leukocyte counts. In this research, one class of adult Wistar male rats were used (n=6), including control group that were fed with enough food and water, sham group and experimental groups (1,2,3) which were fed with %2 solution of cholesterol in oleic acid by gavage for five weeks. Simultaneously, lemon peel essential oil 25µLit/Kg, 50µLit/Kg and 100µLit/Kg were given to the first, second, and third experimental groups respectively, but sham group received saline solution. At the end of this period, blood samples were obtained from the heart ventricles of the rats to measure plasma lipids by enzymatic method, in control, sham and experimental groups. Blood smears were prepared to assess differential leukocyte counts. Data were introduced into SPSS (ver12) software and analyzed by means of Kruskul-walis and mann-whitney U statistical tests. The results of this research showed that the effect of low doses of lemon peel essential oil was slight on decreasing the level of cholesterol and LDL in the experimental group in comparison to sham group (p<0.05) and had no significant effect on the level of triglyceride (p>0.05). However, moderate and high doses of the oil showed significant effects on decreasing cholesterol (p<0.01), LDL (p<0.01) and triglyceride levels (p<0.01). None of these doses resulted in significant effect on the plasma HDL (p>0.05). The study revealed that three doses of essential oil had significant effects on increasing the number of leukocytes (p<0.001). It is concluded that lemon peel essential oil probably can prevent atherosclerosis and decrease incidence of cardiovascular diseases by decreasing plasma lipid levels. In addition, it is likely lemon peel essential oil prevents infectious diseases and cancer by increasing the number of leukocytes and percentage of blood lymphocytes which result in augmentation of immune system.

Background and An important factor involved in infertility is reactive oxygen species (ROS). ROS can damage sperm DNA, and involve lipid per-oxidation. ROS elevation is under the influence of leukocyte activation. The objective of this study was to evaluate the level of ROS as well as leukocyte, in normozoospermic (NO) and ologoasthenoteratozoospermic (OAT) ejaculates. The study population consisted of 75 individuals who were referred to the Research and Clinical Center, regarding infertility in Yazd, for semen analysis. Fifty (50) out of Seventy-Five (75) men were NO, and the remaining was OAT. ROS was measured with laminator, while leukocyte concentration was analyzed with the ENDTZ test The results showed that ROS levels in OAT was significantly higher compared with NO (1253.49±200.95 vs. 75.64±149.52 P=0.00). Furthermore, men with OAT were divided into 2 groups, with sperm morphology and motility >5% and <5%. In group >5%, ROS level was significantly higher than the group with <5% (3627.55±407.79 vs. 81.29±100.48 P=0.007). In addition, leukocyte concentration in NO was 0.07±0.22x106 while it was 0.12±0.20x106 in OAT samples P=0.35). The results indicate although ROS is present in normal seminal samples, it is significantly higher in OAT. This shows the vital role of antioxidants, which may improve the sperm quality. Further clinical studies will pinpoint the antioxidant capacity in improving the seminal contents.