جستجوی مقالات مرتبط با کلیدواژه "لیشمانیا تروپیکا" در نشریات گروه "پزشکی"

Cutaneous leishmaniasis or leishmaniasis in Iran is caused by two species of Leishmania parasites including: L. major and L. tropica. In recent years, the disease has been reported in different parts of Kohgiluyeh and Boyer-Ahmad Province. Due to the huge differences in the type of animal reservoirs and biological carriers, identifying the causative agent can be effective in the necessary planning to control the disease. Therefore, the aim of the present study was to determine the causative species of cutaneous leishmaniasis in Kohgiluyeh and Boyer Ahmad province, Iran, by molecular

a retrospective study. Methods The present cross-sectional and retrospective descriptive study was conducted to determine the species of Leishmania, 52 samples of smears stained with Giemsa dye were collected from different laboratories in the cities of the province between 2013 and 2017 and were examined using light microscope for the presence of Leishman's body. After extracting the DNA present on the surface of slides, using specific primers LINR4 and LIN17, the species of Leishmania parasite was identified by PCR method. The data were analyzed using chi-score statistical test.

Out of 52 samples with cutaneous leishmaniasis lesions, 26 (50%) were male and 26 (50%) were female. The mean age of the participants in the study was 23.32 years (with a standard deviation of ±18.42). Out of 52 samples, parasitic amastigotes (Leishman's body) were observed in 36 cases (69.2%) by direct observation using a light microscope and 43 samples (82.7%) were positive for PCR. Infectious species in all residents of Dehdasht and Yasuj were L. major and L. tropica in Gachsaran.

the results of the present study indicated that L.major and L.tropica species were the cause of cutaneous leishmaniasis in Dehdasht, Yasuj, and Gachsaran. Therefore, further studies are suggested to identify vectors and reservoirs of cutaneous leishmaniasis parasites in Kohgiluyeh and Boyer-Ahmad province, Iran.

Leishmaniasis is a disease in tropical regions, that includes a wide range of clinical protests, from skin lesions to fatal visceral infections. A2 gene is accounted as one of the most reliable genetic factors cause visceral form. This gene is a single copy without functional expression in the species causing skin form of the disease such as L. major and L. tropica. The aim of our study is to evaluate A2 gene sequence in the strains that cause skin form of disease and compare it with other   exception strains of the Leishmania tropica strains in Iran.

Leishmania species were detected using ITS1 and ITS2. The parasites were injected into BALB/c mice and monitored of footpad inflammation in BALB/c mice. after a certain period of time the mice were killed and their visceral organs were examined for the presence of parasites.  Finally, A2 gene sequence analyzed.

A2 gene in a strain causing visceral form was different to a gel electrophoresis pattern of skin form causing strains. Also, the gel electrophoresis pattern of A2 gene in strains causing skin form was different to previous reports of cutaneous leishmaniasis.

The results of this recent experiment showed that the gel electrophoresis pattern of A2 gene in especial strains causing viseral form was similar to previous reports of visceral leishmaniasis. It seems that this gene may play an important role in the visceral ability of specific strains of Leishmania tropica.

Leishmaniasis is endemic in Iran, and Mashhad is one of the most involved cities. Glucantime, prescribed as intralesional or systemic injection, is the most common treatment for cutaneous leishmaniasis. We investigated the additive effect of topical ciprofloxacin on the intralesional injection of glucantime in treatment of cutaneous leishmaniasis.

This randomized clinical trial study was performed on patients with cutaneous leishmaniasis diagnosed by direct smear or biopsy, who referred to Imam Reza and Ghaem teaching hospitals of Mashhad University of Medical Sciences. Patients were randomly allocated into two groups to receive either topical liposomal ciprofloxacin 2% twice daily plus weekly intralesional glucantime or intralesional glucantime alone, for eight weeks. The size of induration and the rate of healing was assessed every week.

There were no significant differences between the groups in cure rate (decrease in induration size equal or more than 75%) (P = 0.410) and the time of healing (P = 0.050) 45 days after the end of treatment.

 Using topical ciprofloxacin does not have any additive effect on the conventional treatment of cutaneous leishmaniasis.

Leishmaniasis (L) has a long history in Iran with three types including: Zoonotic Cutaneous Leishmaniasis (ZCL), Anthroponotic Cutaneous Leishmaniasis (ACL) and Zoonotic Visceral Leishmaniasis (ZVL). This study aimed at epidemiological features of disease from1983 until 2012.

A retrospective cross-sectional study was conducted by using leishmaniasis surveillance data and analyzed in Excel software.

Leishmaniasis cases in Iran from 1983 to 2012 are 569164. Cutaneous Leishmaniasis cases are 566532 (99.5%), with annual average 18884 cases and the annual average incidence rate is 32 per 100,000 populations. ZVL cases are 2632(0.5%), with annual average 175.4 cases and the annual average incidence rate is 0.18 per 100,000 populations, this data have been recorded from all districts in the country.

CL and ZVL have decreased through used by control measures in recent years.

Background and

Recently، a high rate of cutaneous leishmaniasis (CL) has been reported in Torbat-e-Jam a town in Khorasan Razavi province، Iran. The aim of the present study was to investigate the epidemiological aspects of CL in this district.

This descriptive cross-sectional study was carried out in patients referring to health centers of Torbat-e-Jam، during 2009-2011. Diagnosis of CL was made by direct smear and staining was done through Giemsa. Also، specific PCR method was performed for detection of the species of Leishmania spp.

Totally 855 individuals were investigated of whom 504 (58. 9%) were found positive through direct smears. Among the subjects 291 (57. 7%) were male and 213 (42. 3%) were female. The majority (40. 5%) of the positive cases were observed in 2011. The most highly infected cases were aged 5-10 years (22. 3%) and the least rate was seen amongst those aged >40 years of age (2. 6%). Also، Leishmania spp isolated from patients were Leishmania tropica by species specific PCR.

According to the increasing trend of CL، it is believed that a new endemic focus of Anthroponotic CL (ACL) is gradually developing in Torbat-e-Jam. Humans are the main reservoir of ACL، therefore، early diagnosis and treatment among them are of great importance.

Cutaneous leishmaniasis (CL) is a prevalent disease worldwide including Iran. In Iran Leishmania tropica and Leishmania major are two causing factors of cutaneous leishmaniasis and Bam is one of the old and well-known focuses of CL. The objective of the present study was to identify the resistant isolates to meglumine antimoniate (MA) for implementation of future control measures in Bam.

This work has been conducted during 2009-2010 in the city of Bam and Kerman School of Medicine. From a total of 2126 patients with CL, 235 patients (11.1%) were resistant against MA (Glucantime) of whom 51 ones were randomly selected. Skin scrapings were taken for direct smear preparations and culture media and Nested-polymerase chain reaction (PCR) were used for species identification.

In this study, 122 males (51.9%) and 113 females (48.1%), resistant to MA were identified that shows no significant difference between the two sexes. With a significant difference most of the resistant patients were in the age group 11-21 years (29.4%), followed by ≤ 10 years (21.6%) and the lowest were in the age group≥ 55 years (5.9%). Most of the lesions were on face (55.5%), the majority had one lesion (64.5%) and 33.3% received MA intra -lesionally. According to the results of PCR, all 51 isolates were Leishmania tropica.

To our knowledge this is the first study that is carried out on the resistant patients to MA in Bam. Since the incidence of this disease and drug resistance have been increased after the earth quake of 2003, further studies to identify genetic variants of resistant isolates in order to use new alternative drugs are required.