جستجوی مقالات مرتبط با کلیدواژه « adult male rat » در نشریات گروه « پزشکی »

Saffron has anti-seizure, anti-depressant, anti-inflammatory, anti-tumor and memory improvement drugs. In this study, the effect of saffron hydroalcoholic extract on changes in liver-related biochemical factors following chronic vitamin A intoxication in adult male rats was investigated.

For this purpose, 48 adult male Wistar rats weighing approximately 200±20 g were divided into 6 groups of 8. Control group: did not receive any drug treatment, sham group: received only solvent extract, experimental groups 1: Hydroalcoholic extract of saffron alone received 50 mg / kg for 15 days. Experimental group 2: Vitamin A received 50,000 IU for 15 days. Experimental groups 3 and 4: First, they received 50,000 IU of vitamin A for 15 days and then received saffron hydroalcoholic extract at 50 and 80 mg / kg, respectively, for 21 days. At the end of the experiment, the body weight of the animals was measured. Blood samples were taken from the heart and albumin, bilirubin and total protein levels were measured. The results were analyzed using ANOVA and Tukey test.

The mean body weight in the groups receiving vitamin A and saffron hydroalcoholic extract together and alone did not show a significant change compared to the control and sham groups. Mean serum levels of total protein and albumin in experimental group 2 receiving vitamin A and experimental group 3 showed a significant decrease compared to control and control groups. The mean serum levels of total protein and albumin in experimental group 4 showed a significant increase compared to experimental group 2 receiving vitamin A. The mean serum level of total bilirubin in experimental group 2 receiving vitamin A and experimental group 3 showed a significant increase compared to the control and sham groups. The mean serum level of total bilirubin in experimental group 4 showed a significant decrease compared to experimental group 2 receiving vitamin A (P> 0.05).

Saffron hydroalcoholic extract has a protective effect on changes in liver-related biochemical factors following chronic vitamin A poisoning in adult male rats.

Curcumin has antioxidant and anti-inflammatory activities. The aim of this study was to investigate the protective effect of curcumin on changes in the serum concentration of LH, FSH , testosterone hormones and testicular tissue induced by methotrexate in adult male rats.

In this experimental study, 30 adult male Wistar rats with an approximate weight of 230-260 grams were divided into 6 groups of 5 each. Control group, sham group: 1 ml of distilled water as a solvent, experimental group 1: methotrexate (5 mg/kg), experimental group 2: curcumin (400 mg/kg), experimental group 3: methotrexate (5 mg/kg) + curcumin (200 mg/kg), experimental group 4 : methotrexate (5 mg/kg) + curcumin (400 mg/kg). After 28 days of treatment in the form of gavage injection, blood was taken directly from the heart to measure the serum concentration of  LH, FSH and testosterone hormones. The testicles were removed and tissue changes were examined after hematoxylin-eosin staining. The data results were analyzed based on one-way analysis of variance (ANOVA) and Tukey's test.

In the group receiving methotrexate, the level of testosterone hormone, the number of spermatogonia, spermatocytes, sperm and leydig decreased and the serum level of LH and FSH increased significantly compared to the control and sham groups. In the groups receiving methotrexate + curcumin, curcumin was able to compensate the adverse effects caused by methotrexat (P <0.05).

Curcumin can improve the changes in serum testosterone, FSH, LH concentration and testicular tissue induced by methotrexate in adult male rats.

Primidone is effective in the treatment of epilepsy. In this research the effects of primidone on LH, FSH, testosterone hormones, body weight and testicular tissue in adult male rats, was studied.

In this experimental study, 50 adult male Wistar rats, weighing approximately 180-200 g were divided into 5 groups of 10 animals. Groups: control group, Sham group and treatment at doses of 20, 40, 60 mg/kg, orally for 42 days (every other day) respectively. At the end, after determining the animal's body weight, blood samples was collected from the heart. Hormone concentrations of LH, FSH and testosterone were measured by RIA.Their testis were removed and weighed to evaluate the histological changes after hematoxylin-eosin staining.

The body weight, testis weight and leydig and sertoli cells in all experimental groups showed no significant changes compared to the control group. The plasma levels of LH and FSH in all experimental groups increase significantly compared to the control group. The plasma level of testosterone Hormone and number of  spermatogonia,primary spermatocyte and spermatid in all experimental groups decrease significantly compared to the control group (P<0.05).

primidone reduced levels of testosterone and increase levels of LH and FSH hormones.

Primidone is effective in the treatment of epilepsy. In this research, effect of primidone on liver- related biochemical factors in adult male rats was studied.

For this purpose, 40 adult male wistar rats, weighing approximately 180-200 g were divided into 5 groups of 8 animals. Groups: control group, Sham group and treatment at doses of 20 ,40 and 60 mg/kg, orally for 42 days (every other day) respectively. At the end of the experiment , liver weight were measured in all groups and then the blood sample was taken from heart and serum levels of albumin, total protein and total bilirubin were measured. The results were analyzed using ANOVA and tukey test statistical analysis.

the results show that liver weight in experimental groups had not a significant difference compared to control group. Serum level albumin and total protein in experimental groups had a significant decrease compared to control group. Serum level total bilirubin in experimental groups had a significant increase compared to control group (p≤0.05).

Primidone decrease serum levels of albumin and total protein and increase serum level of total bilirubin.

Primidone is effective in the treatment of epilepsy. In this research, effect of primidone on levels of liver enzymes and liver tissue change in adult male rats was studied.

For this purpose, 40 adult male wistar rats, weighing approximately 180-200 g were divided into 5 groups of 8 animals. Groups: control group, Sham group and treatment at doses of 20, 40, 60 mg/kg, orally for 42 days (every other day) respectively. At the end of the experiment , body weight were measured in all groups and then the blood sample was taken from heart and serum levels of AST, ALT, ALP and GGT enzymes were measured. Furthermore, The pathological changes of the livers were studied after hematoxylin-eosin staining. The results were analyzed using ANOVA and T-test statistical analysis.

the results show that body weight in experimental groups had not a significant difference compared to control group. Serum level in AST, ALT, ALP, GGT in experimental groups had a significant increase compared to control group (p≤0/05). In tissue samples in experimental groups which were provided a higher level of necrosis was observed.

Primidone causes increase serum levels of AST, ALT, ALP, GGT and liver necrosis.

Food restriction (FR) affects on depression and anxiety behavior. Previous studies have pointed to the role of sulpiride in the treatment of depression and anxiety. The purpose of this study was to determine the role of dopaminergic system and food restriction on depression and anxiety. Forty two adult male Wistar rats (180-220g) were divided into 7 groups including: control, sham, 25%, 50%, 75% FR, 75% FR with sulpiride and sulpiride(50µg/rat). The treatment period was 21 days. Day 19 surgery and cannulation was performed and Sulpiride was injected intracrebroventricularly (icv) on the 21st day. Depression and anxiety tests (Tail suspension test and Elevated Plus Maze), was taken from the rats on the 21st day. Data were analyzed by ANOVA test.  Ethical points were observed according to the declaration of Helsinki and acts of Bioethics Committee at Urmia University (Ref No: AECVU-180-2018). Mobility time in FR groups and sulpiride compared to control and in 75% with sulpiride compared to 75% had significant increase. immobility time in FR and sulpiride had significant decrease compared to control. Open arm entry in 75% FR had significantly decreased compared to control and in 75% FR with sulpiride had significantly increased compared to 75%. Open arm spend time in 75% FR with sulpiride had significant increase compared to 75%. Motor activity was reduced in FR groups and injection sulpiride. It seems that sulpride and FR play a role in improving
semi-depression circumstances. Sulpiride also play a role in controlling the anxiety behavior induced by FR.

Taraxacum Officinale is a medical plant with antioxidant benefit effects. The aim of the present study is to evaluate the effect of Taraxacum Officinale alcoholic extract on serum thyroid hormone levels in adult male rat.
Material and

In this experimental study,45 adult male wistar rats were approximate average weight body of 200-250 gr were divided in to 5 groups of 9. Control ,sham ,and three experimental groups which received 50,100,150mg/kg of Taraxacum Officinale alcoholic extract by used gavage method. After this period (14 days) blood sample were collected and after measuring the amount of thyroid hormones of T3,T4,TSH hormones, data were analyzed by using SPSS software , ANOVA and t-test.

The results showed that body weight average and serum level of T4 hormone in receiving maximum dose of Taraxacum Officinale alcoholic extract showed significant decrease comparing to control group. T3 serum level in experimental groups receiving Taraxacum Officinale alcoholic extract didn't show any significant difference comparing to control group. TSH serum level in experimental groups receiving Taraxacum Officinale alcoholic extract showed significant decrease comparing to control group (p<0.05).

The Taraxacum Officinale alcoholic extract reduced serum thyroid hormone levels in adult male rat

The liver has important role in the metabolism of chemical drugs and plasma protein synthesis. Abilizol is used in the treatment of schizophrenia and bipolar maniac disorders. In this study the effect of abilizol have been observed on serum levels of liver enzymes, total protein, albumin and liver tissue changes.
In this experimental study 50 adult male rats from wistar race were divided in to 5 groups of 10.The experimental groups which received 5, 10 and 15 mg/kg of abilizol drug orally for 28 days. The control group which received nothings and the sham group which received solvent. At the end of this Period, blood samples were taken from all groups. ALT (Alanine Transaminase), AST (Aspartate Transaminase), ALP (Alkaline phosphatase), total protein and albumin were measured. Also histological changes of liver studied.
No significant differences were observed in levels of ALT , ALP and albumin enzymes between experimental and control groups. In experimental group received 15mg/kg abilizol drug showed a significant decrease in level of AST in compared with control group. In experimental group received 15mg/kg abilizol drug showed a significant decrease in level of total protein in compared with control group (p≤0.05). Density of liver tissue in all experimental groups received abilizol drug decrease significantly in compared with control group.
Results show that abilizol is a cause of liver parenchymal cell failure and decreases liver enzymes levels.

Background and Ducrosiaanethifolia is an aromatic medicinal plant native to Iran, and has been used in traditional medicine for controlling infection, reducing anxiety and pain. Since analgesic effect of this plant has not been studied experimentally, the aim of the present research is investigating the analgesic effect of Dc. essential oil. In this experimental study, 48 adult Wistar male rats were examined. Rats were divided randomly into 6 groups (n= 8) including: control group, morphine group and Dc. essential oil group (0.06, 0.125, 0.25 and 0.5 ml/kg, IP). Antinociceptive effects of drugs were assessed using hot plate apparatus. The results were analyzed using SPSS using one-way analysis of variance (ANOVA) followed by post hoc Tukey. Differences were considered significant at p< 0.05. The Dc. essential oil (0.5 ml/kg) significantly decreased sensitivity to painin comparison with control group. Latency to onset of pain significantly increasedby the Dc. essential oil (0.125 ml/kg) 60 and 120 minutes after injection compared with control group. Also, the Dc. essential oil (0.25ml/kg) reduced pain 120 minutes after injection in comparison with control group. Based on the findings of present study, the Dc. essential oil has analgesic properties and this plant can lead to decreased sensitivity to pain at some doses in the hot plate model of pain.

Stress has different effects on the living organisms. In this study the effect of immobilization stress the HPG (hypothalamic - pituitary - gonad axis) hormones secretion and the number of spermatogonia was studied in adult male rats. 30 male rats were divided into 3 groups. The Control group: who were not exposed to any kind of stress، and two experimental 7 and 14 day groups which respectively received 2 hours of immobilized stress over 7 and 14 day stress periods، blood samples were collected directly from the heart of the animals، Hormones GnRH، LH and testosterone levels were measured by ELISA. The number of spermatogonia in the testes of adult male rats were counted. We used ANOVA and Duncan''s test results in p ≤0. 05 for statistic analyze test. The results showed that immobilization stress significantly decreased concentrations of GnRH and testosterone hormones compared with the control group. Also the 14-day immobilization stress caused a significant reduction in spermatogonia cells. The results of this study indicate that the inhibitory effect of immobilization stress on the HPG axis may also cause negative effects on the spermatogenesis process.

Among the trophic factors in CNS, the role of neurotrophins for their multiple actions is more pronounced. The purpose of this study was to determine the effect of two weeks light aerobic running on protein levels of Brain Derived Neurotrophic Factor (BDNF) and the specific receptor of TrkB, relying on the more transparent role of exercise on synaptic and neurogenerative modification.

This experimental study was conducted with the animal model. Twelve adult male wistar rats, 8 weeks of age, were selected as subjects (with mean body weight of 200-225 gr). The animals were randomly divided into 2 groups of control (n=6) and runner (n=6). In runner group, animals were allowed to run on treadmill at a speed of 12 m/min daily for 30 minutes for 2 weeks. Twenty four hours after the last session of exercise, the animals were sacrificed and the hippocampus of both sides of hemisphere removed. Changes in protein levels were determined with ELISA technique.

Statistical analysis by independent sample t test showed that between the runner and control groups there was a significant difference (p≤0.05) statistically and running significantly increased the protein levels of BDNF and TrkB in the hippocampus of rats.

Increase in these factors shows the effect of exercise as a positive moderating factor in the growth and survival of neuronal and synaptic plasticity.

Transcription factors (TF) and microRNAs, are the largest families of transacting gene regulatory molecules in multicellular organisms. Our goal was to examine the effect of aerobic running on the expression of miR-124 and RE1-silencing TF in the hippocampus of adult male Wistar rats. A total of twelve 8-week-old adult male Wistar rats with a mean body weight of 200-225 g were selected as subjects. Following 1 week of familiarization, the animals were randomly divided into two groups of test (n=6) and control (n=6). In the test group, animals were forced to run on a treadmill, at a speed of 25 m/min for 30 minutes per day, for 14 consecutive days. The animals were sacrificed 24 hours after the last exercise session, and the hippocampi were removed from both sides of the brain hemispheres. Changes in the expression of miR-124 and RE1-silencing TF were analyzed using the quantitative RT-PCR technique. Statistical analysis by independent sample t-test, showed that there was a significant difference between the exercise and control groups (P≤0.05), and while exercise significantly elevated the expression of miR-124, it reduced the expression of RE1-silencing TF. Our findings show that forced aerobic running at a speed of 25 m/min could lead to positive changes in mechanisms involved in exercise-induced neurogenesis.