جستجوی مقالات مرتبط با کلیدواژه "antibacterial activity" در نشریات گروه "محیط زیست"

Wound infection can cause delayed wound healing, and due to the emergence of antibiotic resistance, treatment of wound infection has become less effective using conventional antibiotics. This has led to the search for an alternative treatment method, which ethnomedicine has proven to be promising. Thus, the antibacterial activity of Senna occidentalis leaf extract was evaluated against isolated bacteria from the wound of patients attending Kwararafa Hospital Wukari, Taraba State, Nigeria. A total of three (3) wounds were randomly swabbed. Bacteria were isolated and identified using standard microbiological procedures. The disk diffusion method was used to assess the antibacterial activity of the aqueous and methanolic extracts of Senna occidentalis leaf extract at different concentrations (20 mg/ml, 40 mg/ml, 60 mg/ml, 80 mg/ml, and 100 mg/ml). The bacteria pathogens isolated and identified are Staphylococcus spp. 3(37.5%), Klebsiella pneumonia 2(25%), Escherichia coli 2 (25%), and Pseudomonas aeruginosa 1(12.5%). The aqueous extract was not effective, while the methanolic leaf extract of the plant displayed promising antibacterial activity at all concentrations against all the bacterial isolates, with the highest zones of inhibition recorded at 100mg/mL, measuring 16±5.0mm, 18±5.0 mm, 20±5.0 mm, and 15±0.0 mm, for Staphylococcus spp., Klebsiella pneumonia, Escherichia coli, and Pseudomonas aeruginosa respectively. The result of this study supports the traditional use of Senna occidentalis in the treatment of wound infections.

This study investigated the antibacterial activity of a lectin-like bacteriocin extracted from Pseudomonas putidaisolated from various soil samples in Baghdad. Thirteen new isolates of P. putidawere identified using morphological, physiological, and molecular methods. Real-time PCR identified three isolates carrying the bacteriocin gene. Antibiotic sensitivity was tested on pathogenic bacteria, and one isolate from each species exhibiting broad-spectrum resistance was selected. The crude bacteriocin from 13 isolates showed antimicrobial activity against six pathogenic bacterial species, with five isolates exhibiting inhibition zones. Purification resulted in a bacteriocin with a total protein concentration of ~4669μg/ml and an apparent molecular mass of ≤11 kDa. HPLC confirmed the molecular mass. This study quantified the expression of P. putidabacteriocin genes, characterized as putadicin, with potential applications in human medicine for burn treatment in Iraq.

The objective of the present study was to assess the antibacterial and antibiofilm activities of Pediocin, a bacteriocin extracted from cheese, versus a subset of bacteria linked to foodborne diseases. The agar well diffusion method was used to evaluate the antibacterial activity of Pediocin. The results showed that Pediocin had a potent bactericidal effect against all the tested bacterial strains. The crystal violet staining method was used to assess Pediocin's antibiofilm efficacy, and the results showed that Pediocin significantly inhibited the development of biofilms by the tested bacterial strains. Investigating the effects of temperature, pH, and medium make-up improved Pediocin output. It was discovered that a temperature of 30 °C, a pH of 6.5, and a medium made up of tryptone, yeast extract, and glucose were the ideal conditions for the creation of pediocin. Pediocin demonstrated significant antibacterial activity against all the tested bacterial strains, with the highest activity against Staphylococcus aureus, Streptococcus pyogenes and Helicobacter pylori. The minimum inhibitory concentration (MIC) values of Pediocin against the tested bacterial strains varied from 0.5 to 32 µg/mL. Among them, the lowest MIC was observed against S. aureus, P. aeruginosa, and K. pneumoniae. Similarly, the minimum bactericidal concentration (MBC) values ranged from 2 to 64 µg/mL, with the lowest MBC observed against S. aureus, P. aeruginosa, and K. pneumoniae. The large-scale manufacture of Pediocin, which may be utilized as a natural food preservative to stop the growth of pathogenic bacteria and lower the frequency of foodborne diseases will be made easier by the optimized production circumstances. The potential of Pediocin for food preservation and the security of its use require more research.

Antimicrobial resistance in Klebsiella pneumoniae has been on the increase over the years Therefore, numerous endeavors have been carried out so as to discover another active plant combination as a suitable substitute for antibiotics. This is a Experiment of the activities of antibacteria from the extract from Rumexa alveoli against ebsiella pneumonia.

This study was performed on the standard strain of Klebsiella pneumonia and several clinical strains. The aqueous and ethanol extracts of Rumex alveollatus; formulated by the Soxhlet apparatus. The activities of the antibacterial of the extracts were analyzed with the use of the disk-diffusion method. Furthermore, the minimal-inhibitory-concentration (MIC) and the MBC were examined by the micro dilution method. The database collected by SPSS recent software was evaluated.

Inhibitory zone of ethanol (500 mg/ml) on standard and clinical strains of Klebsiella pneumoniae were 18, 17, 17,15,16, and 16 mm, respectively, and also aqueous extract did not have an antimicrobial effect. MIC and MBC results for ethanol extracts were 125 and 250 mg/ml, respectively.

The antibacterial activities of the Rumex alveollatus ethanol and aqueous extract tested against bacteria was good . Thus, the findingss of this research can be a beneficial report concerning their active role in infection control.

The study for new antibiotics is of great importance in investigating programs around the worldwide for pharmaceutical, industrial and agricultural applications. Streptomyces like filamentous soil bacteria are used as an essential biological tool for their ability to producing a wide range of new secondary metabolites such as antibiotics. Identification and isolation of new species seemed to be important in the presentation of significantly adequate antibiotics, because antibiotic resistance infectious diseases are the second leading cause of death worldwide, inducing research and development of new antibiotics. Therefore, in this study, we aimed to isolate and characterize novel strains of Streptomyces spp. with high antibiotic production ability.

Soil samples were collected randomly from primitive soils of Urmia, West Azerbaijan province, from Iranin 2019. The isolates of Streptomyces spp. were carried out in a specific culture medium. Their primary and secondary antibacterial activity againstgram-positive bacteria Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus, alsogram-negative Escherichia coli was checked out. Finally, the antibacterial properties of strains based on 16S rRNA sequencing were analyzed by MEGA X software.

Totally, 150 colonies were isolated from four soil collected samples. In the primary screening of 10 isolates, insulated antibacterial activity and in the secondary screening, 3 examples were selected. The microorganisms showed antibacterial activity. Sequencing of the 16S rRNA gene from C-B1-12, D-D3-7, and C-Y2-2 isolates showed similarity to Streptomyces indiaensis.

The results of this study indicated that there are new isolates in the soil samples of West Azerbaijan province that are capable of producing new antibacterial agents.

The present study was carried out to determine the potential antibacterial effect of essential oil of Origanumvulgare against antibiotic resistant E.coli. In this study, the essential oil of Origanumvulgare obtained by hydrodistillation was analyzed by gas chromatography coupled to mass spectrometry (GC-MS) in order to determine their chemical composition. The minimum inhibitory concentrations were investigated to characterize the antimicrobial activities of this essential oil. The results in tables 1 showed that essential oil of Origanumvulgare had inhibitory effect against most isolated plates. The least MIC value of essential oil of Origanumvulgare was 0.62 mg/ml and the highest MBC value of essential oil of Origanumvulgare were 5 mg/ml and 10mg/ml. The present studies confirm the use of this essential oil as antibacterial agent. Further research is required to evaluate the practical values of therapeutic applications.

Vibrio cholerae is a human pathogen that causes mild to severe diarrheal illnesses and has major public health significance. The aim of this study was to investigate the effects of antimicrobial activity of the gold nanoparticles on Vibrio cholera. Gold and Silver nanoparticles are chemically synthesized. Standard strain of Vibrio cholerawas cultured in a nutrient broth. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was determined by micro dilution.

The aim study antibacterial effects of silver nanoparticles against resistant strains of E.coli bacteria.12 strains of E. coli strains arising urinary infection were isolated from hospitalized patient in zabolhospitals. Minimum inhibitory concentration of winter cherry were determined by dilution method in various concentration on bacteria. The highest MIC values was found to be 100ppm against one E.coli and the least MIC values was observed in 12.5 ppm. This work, integrates nanotechnology and bacteriology, leading to possible advances in the formulation of new types of bactericides.

Antibiotic resistant to Antimicrobial agent is one of the most important concern in hospitals, which can cause lots of costs, treatment fails and mortality rates. Acinetobacter baumannii is a Gram-negative bacterial pathogen notorious for causing serious nosocomial infections that resist antibiotic therapy. The purpose of current study was to define the evolution of antimicrobial impact of flower extract of Hibiscus sabdariffalagainstAcinetobacterbaumannii. Hibiscus tea plant after collecting dried and then milled, their extraction was performed using vacuum from the center (Rotary). The Twelve strains of Acinetobacterbaumannii were isolated from patients in the city of Zabol. Minimum inhibitory concentrations and MBC of the extract was determined on bacteria in different concentrations by dilution in the wells. The result revealed that the levels of MIC range were from 6.25 to 25ppm. The highest MIC value was 6.25 ppm against A. Baumannii and Acinetobacterbaumannii were resistance to 4 of the agent including nalidixic acid(100%), penicillin(100%), tetracyclin(58.3%), amikacin(83.3%). In important human pathogens, drug resistance is increasing according to the results of this study may be proposed that this plant can be used as a drug. It can be a good way to replace herbs with chemical drugs.

The purpose of this study was to examine the evolution of antimicrobial activity of flower extract of Hibiscus sabdariffal against antibiotic-resistant Escherichia coli and Staphylococcus aureus that isolates from the urinary tract infection by microtiterplate method. All 42 strains 30 E.coli and 12 S. aureus isolated from urine culture of hospitalized patients in Zabol ( Zabol, south-eastern Iran) suffered from urinary tract infections during the years 2011- 2012 were evaluated and the extract of Hibiscus sabdariffalobtained by rotary and the minimum inhibitory concentrations were investigated to characterize the antimicrobial activities of this extract. The result show that E. coli were resistance to 4 of the agent and more resistance to tetracyclin(63.3%), erythromycin(56.6%) and cefixime(40 %), S.aureus  more sensitive were antibiotic resistance to vancomycin and cefixime. The highest MIC values was found to be 20mg/ml against two E.coli.  The leas MIC values was found to 1.25 mg/ml against three S.aureus.

In this study, Invitro evaluation of some Iranian plants against Staphylococcus aureus Isolation of Urinary Tract Infection by microtiterplate method. All 12 strains of Staphylococcus aureus isolated from urine culture of hospitalized patients during the years 2012- 2013. Minimum Inhibitory Concentration (MIC) of plant extract and essential oilagainst bacteria were determined using micro dilution broth method at six different concentrations. The result showed that the antibiotic susceptibility of S.aureus isolates was evaluated for 6 antimicrobial.Antibiotic susceptibility of S.aureus isolates was evaluated for 6 antimicrobial and more resistance were to oxacillin(83.3%), ceftazidime(66.6%) and penicillin(50%). The highest MIC values of extract were found to be 2.5 mg/ml against S.aureus andone of MIC value for S.aureus was 0.62mg/ml. This study also confirm the antimicrobial potential of investigated plants and their usefulness in treatment of resistance microorganisms gram-positive.

The aim of this study was to evaluate the antimicrobial activities of Trachyspermum ammi, essential oil against different kinds of microorganisms by microtiter plate method. All 36 isolates of (12 Klebsiella pneumoniae, 12 E.coli and 12 Staphylococcus aureus) isolated from urine culture of hospitalized patients (Amir Al-Momenin Hospital, Zabol, south-eastern Iran) suffered from urinary tract infections were evaluated. In this study, the essential oil of Trachyspermum ammi obtained by hydrodistillation for 2.5-3 h using a Clevenger-type apparatus and the minimum inhibitory concentrations were determined to characterize the antimicrobial activities of this essential oil. The results showed E. coli isolates were resistance to 4 of the antibiotics including ceftazidime (50%) cefixime (41.6%), tetracyclin(75%), erythromycin(58.3%). However k. pneumonia isolates were resistance to 3 of the agent including ceftazidime(33.3%) ,cefixime(58.3%), erythromycin(75%) and S.aureus isolates  were resistance to 6 of the agent including cefixime (33.3%), trimethoprim-sulfamethoxazol(41.66%), penicillin(50%), oxacillin(3.3%), ceftazidime(66.6%) and vancomycin(8.3%)  and the MIC value was also determined against all the tested bacteria. The highest MIC values of essential oil were determined 100ppm against E. coli and highest MIC value for K.pneumoniae was 250ppm. In conclusion, it seems that Trachyspermum ammi essential oil could inhibit the growth of all of the tested bacteria.