جستجوی مقالات مرتبط با کلیدواژه « بقای سلولی » در نشریات گروه « پزشکی »

Amygdalin is a cyanogenic compound naturally present in apricot, peach, bitter almond, plum and apple seeds. The aim of this study was to evaluate the effect of amygdalin on cell survival and Malondialdehyde (MDA) level in the SK-BR-3 breast cancer cell line and to compare these effects on the healthy MRC-5 cell line.

Breast cancer cell line SK-BR-3 and healthy cell line MRC-5 were used in this study. To study the effect of amygdalin on the survival of these two cell lines and on the surface of MDA, concentrations of 2.5, 5, 10, 20, 40 and 80 mg/ml of amygdalin were used. Cell survival was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and MDA level was assessed by thiobarbituric acid and spectrophotometric method. Statistical comparison of the mean between the groups was performed using a one-way analysis of variance (ANOVA) followed by a post hoc Tukey's test.

Based on the results of the present study, amygdalin in a dose-dependent manner significantly reduced SK-BR-3 tumor cell survival and also significantly increased MDA levels in this cell line (P <0.05), but it there were no statistically significant effect on the normal survival of MRC-5 cells and MDA levels (P >0.05).

Amygdalin kills SK-BR-3 breast cancer cells by increasing the level of MDA in these cells.

Intracellular reactive oxygen species (ROS) play an important role in cancer stem cell (CSC) function. Hesperetin (Hst) is a flavonoid that has been shown to affect cellular ROS level. The goal of this study was to investigate the effect of Hst on the level of ROS in gastric CSCs (GCSCs). MTT assay was used to evaluate cell survival. Cellular ROS level was measured using 2′,7′-dichlorofluorescin diacetate by flow cytometry. A significant decrease in the survival of GCSCs treated with Hst was observed. Hst decreased ROS level to about 47% and 40% at concentrations of 200 and 400 mM, respectively. It can be concluded that Hst reduces the survival and ROS level of GCSCs and may be influences the function of these cells such as proliferation and differentiation as well as response to chemotherapy by affecting cellular ROS level.

Mesenchymal stem cells are pluripotent stromal cells which are capable of differentiating into different cell lines. Nowadays, umbilical cord Wharton’s jelly (UC-WJ) are increasingly used as sources of stem cells. Studies show that scaffolds can affect the differentiation of stem cells to different cells and cause higher cell viability and proliferation as well. The present study aimed to evaluate the adhesion and viability of WJ-MSCs to PLA/Wax scaffold.

PLA/Wax scaffold was prepared using electrospinning method. Adhesion and viability of MSCs on this scaffold was investigated using scanning electron microscope (SEM) and MTT assay respectively.

SEM results showed that the fibers were homogeneous, uniform, and free of beads with high quality property, and adding wax to PLA significantly reduced the diameter of the nanofibers. These studies confirmed that the cells were attached to the scaffold in large numbers and with appropriate size. The results of MTT show good biocompatibility of the scaffold made with the cells and a significant increase in the survival rate of mesenchymal cells was observed during the period.

In conclusion, using PLA/Wax scaffold has promoted the attachment, survival and proliferation of the cells and has the potential to be an important candidate for developing the efficiency of 3D-cultures in order to cure diseases.

Glioblastoma tumors are the most invasive brain tumors with the origin of neural tissue in the brain. Because of the low success rate of treatment for this type of tumor, the need for a search for new therapies is justified. Glacium flavum is widely used in the pharmaceutical industry due to its richness in alkaloids. In this study, the toxic effects of hydroalcoholic extract of Glacium flavum on glioblastoma cell line (C118) and neuronal neurosphere (NCs) were investigated.
First, The extract of Glacium flavum has been distilled. Neuronal precursor cells from cortex of 17-day-old pregnant mouse fetus were extracted by enzymatic digestion. Then, at concentrations of 100, 300, 500 and 1000 µg/ml of Glacium flavum, the cells were treated at 24 and 72 hours intervals. After treatment, the viability of the cells was evaluated by MTT method and morphology of the cells by DAPI staining.
The experimented cells were morphologically modified after treatment with Ic50 concentration of Glacium flavum. The evaluation of cell viability by MTT showed that Glacium flavum significantly reduced the viability of C118 cells as well as neurosphere (NCs) in both 72 and 24 hours intervals. The severity of Glacium flavum cytotoxicity on glioblastoma cells was higher than neurospheres and showed a significant difference (P <0.05). Also, the quantitative results of the cell viability indicated that concentrations of 300 and 500 µg/ml of the extract of Glacium flavum has been as the IC50 concentration for C118 cells and NCs respectively.

Finally, it can be concluded that natural products such as Glacium flavum with its antioxidant and anti-inflammatory pharmacological and biochemical properties and as regards it has less site effects on non-cancerous cells so, can be effective in treating glioblastoma tumors.

Industrialization of communities has resulted in a rise in cadmium accumulation in the plants, and also organs of human and animals. Kidney is the main target organ for cadmium accumulation and toxicity. The aim of this study was to investigate the effects of montelukast on cadmium toxicity in renal cells.

This experimental study was performed on human embryonic kidney cells of HEK-293 class. The study included six groups. Group one was our control group. Group 2:  treated with different concentrations of cadmium chloride; group 3: treated with different concentrations of montelukast; group 4: exposed to cadmium chloride IC50 for 24 hours, then treated with montelukast at therapeutic concentration for 24 and 72 hours; group 5: treated with montelukast at therapeutic concentration for 24 hours followed by cadmium chloride IC50 for 24 and 72 hours; group 6: simultaneously exposed to cadmium chloride IC50 and montelukast at therapeutic concentration for 24 and 72 hours. Cell viability and changes in the cell nuclei were determined at specific times by MTT test and DAPI staining, respectively. Finally, the data were statistically analyzed by ANOVA.

Montelukast administration resulted in a significant increase in the viability of cadmium exposed kidney cells. The results of MTT test and DAPI staining showed that treatment with montelukast decreased cadmium induced cell deformation and led to significant improvement of the damaged cells (p<0.05).

Considering the beneficial effects of montelukast on renal cells, it can be recommended for prevention or treatment of cadmium toxicity.

Evaluation of cell viability is momentous in pharmacologic and oncological research. Cell viability evaluation determines cell sensitivity and consequently treatment outcome. Various methods are available to determine cell survival. Each of these methods evaluates different endpoints. Accordingly, determining the correlation between these methods is important. In this study, in order to determine the viability of human anaplastic thyroid cancer cell line, the sensitivity of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, trypan blue test and clonogenic assay were compared. This experimental study was performed in the Cellular and Molecular Research Center at Guilan University of Medical Sciences, Rasht, Iran from October 2016 to March 2017. The human anaplastic thyroid cancer cell line was cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS). The cultured cells were treated with melatonin, for 24 hours. Then, the viability of the cells was evaluated by MTT assay, trypan blue test and clonogenic assay. Furthermore, plating efficiency and surviving fraction were used in order to draw survival curve in the clonogenic assay. The concentration of melatonin at IC50 point was 4.794±0.117 millimolar (mM) in MTT assay, 4.375±0.894 mM in trypan blue test and 2.246±0.326 mM in clonogenic assay. Comparing the IC50 values of these test revealed that C50 values obtained from MTT assay and trypan blue test had no significant difference (P=0.6446), while there was a significant difference between IC50 values obtained from MTT and clonogenic assays (P=0.0032). Moreover, the IC50 values obtained from trypan blue test and clonogenic assay were also significantly different (P=0.0078). The results of the regression analysis of cell viability were shown a linear, positive and significant correlation between these three methods and MTT assay and trypan blue test showed higher correlation (r=0.99, P<0.001). Based on our results, all these methods were effective to identify cytotoxicity in human anaplastic thyroid cancer cell line, while MTT assay and trypan blue test were more sensitive than clonogenic assay.

Phenolic compounds form an important category of antioxidants that have important biological activities, such as anticancer, antifungal, antibacterial and antiviral effects. In this study, the anticancer effects of Gallic acid and P-Coumaric acid were studied on MDA-MB-231 human breast cancer cell line. In this experimental study, human breast cancer cells of MDA-MB-231 (104 cells) were treated with Gallic acid, P-Coumaric Acid in different concentrations (0, 10, 25, 50 and 100 μg / ml) for 24 hours. The cell viability of treated cells was determined using MTT assay and invert light microscopy. The cell viability of treated cell with 100 μg/ml Gallic cell is about 16%, and in the case of para-coumaric acid at the similar concentration, the cell viability is about 83%. The morphological study confirmed the results of MTT using an inverted light microscope. The inhibitory effect of Gallic acid in MDA-MB-231 human breast cancer cells is higher in comparison to P-Coumaric acid at the similar concentration. According to the study, this effect could be due to the difference in the number of hydroxyl groups on these compounds

MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] is a sensitive and accurate method to determine survival fraction of irradiated cancer cells. The aim of present study was to evaluate the radiosensitivity of cancer cells X-ray Irradiation in comparison to normal cells using the MTT assay.
Four cancer cell lines were used, the mouse breast 4T1 cells, the mouse fibroblast L929 cells, the human gastric AGS cells, and the human dermal fibroblasts (HDF) cells. For each cell line, MTT assay was carried out after irradiation to 0, 2, 4, and 6 Gy. For MTT assay, the relationship between absorbed dose and cell number, optimal seeding of cell number, and optimal timing of assay were determined. Then, MTT assay was performed when the irradiated cells had regained exponential growth, or when the non-irradiated cells had undergone doubling times.
Findings: With increasing radiation dose, the mortality of the cells increased, and radiation blocked cell growth.
The response of different cells to irradiation was different. MTT assay may successfully be used, and also may distinguish cell responses to different photon energies. MTT assay was undertaken with optimal assay conditions, and showed the sensitivity of cells to irradiation with regard to the plating efficiency of each cell line, and doubling time at least.

Head and neck cell carcinoma cell lines (HN-5) are considered as an appropriate preclinical model for new therapeutic purposes for this type of cancer. Unlike other cancers, Various primary and metastatic HN5 cell lines are available. The aim of this study was to investigate the anti-cancer effect of nano zinc oxide (ZnO) on viability of malignant HN5 cell line.
In this experimental study, HN5 cell line was cultured in DMEM medium (containing 10% FBS and penicillin/streptomycin) at 37°C. Then, the effect of different concentrations of ZnO on these cells, were assessed by MTT and DAPI staining. The results were evaluated with completely randomized block design ANOVA.
In this study, some concentrations of ZnO were effective in cytotoxicity of HN5 cancer cells. Also, 300μg/ml was the concentration of the studied compound that reduced the highest percentage of cell viability.
The results of this study showed that ZnO has ability to induce cytotoxicity in HN5 cancer cell line in higher concentrations (about 300µg/ml), that these findings These findings provide a new perspective on the use nanoparticles in the cancer chemotherapy.

In order to enhance in vivo stem cell viability and considering similar anti-inflammatory and anti-apoptotic effects of human adipose derived stem cells (hADSCs) and astaxanthin (AST) and their ability to cross the blood-brain barrier, we investigated the effects of (AST) on hADSCs proliferation and viability to provide a supplement for cell based therapy in MS patients.
After isolation of hADSCs and assessment of CD markers, they were cultured in DMEM-F12 medium in the presence of AST at various concentrations (1, 5, and 10 ng/ml) for 72 h. Finally, we assessed cell proliferation and cell viability by MTT and Tryphan Blue methods.
The results revealed that a high percentage of hADSCs expressed CD90 and CD44 markers and a low percentage of them expressed hematopoietic cell markers. In addition, in the group cultured in the presence of 5 ng/ml AST the mean percentage of cell viability increased significantly compared to other groups (p = 0.04). Tryphan Blue results also revealed significant effect of AST on stem cell proliferation and culture of these cells in the presence of 5 ng/ml of AST, led to significant increase in the mean percentage of cell count compared to the results of the control group (p = 0.03).
Astaxanthin can increase hADSCs proliferation and survival and this agent can be used in the cell-based therapies in MS patients.

One of the major causes of death in the world is cancer and therefore any study in the field of cancer biology is of great importance. Head and neck cancers represent approximately 2-5% of neoplasms which is higher in some countries. The most appropriate therapy for various cancers is identifying effective and efficient ways that contribute to initiation of apoptosis. Cyclophosphamide is an alkylating agent that stops the replication of DNA and then, it stops the cell proliferation and viability. Therefore, cyclophosphamide is used to treat various types of cancer. In this study we evaluate the cytotoxic effects of cyclophosphamide on viability of (head and neck cancer cells) HN5 cell line and compare it with fibroblast cells as noncancerous cells.
This experimental study was done in cell and developmental laboratory in faculty of science, Shahid Chamran University of Ahvaz in Spring of 2016. HN5 cell line and embryonic fibroblast cells were grown in DMEM supplemented with 10% fetal bovine serum (FBS), penicillin/streptomycin (100 U/ml, 100 µg/ml) at 37 °C, then the effects of different concentrations of cyclophosphamide on cell viability was evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. 4',6-diamidino-2-phenylindole (DAPI) staining was performed to determine the proportion of apoptotic cells by manually counting pyknotic nuclei. According to standard procedures from day 13 embryos of outbred strains naval medical research institute (NMRI), fibroblast cells were isolated. In this study HN5 cell line and fibroblasts were exposed to cytostatics for 72 hours.
Various concentrations of cyclophosphamide were effective in cytotoxicity of HN5 cancer and fibroblast cells. A significant cytotoxicity was observed with the examined concentration of 1 µg/ml of cyclophosphamide with 50% in 3th day and P Totally cyclophosphamide had low toxicity effects on both of the cell lines but the toxicity effects of cyclophosphamide on HN5 were significantly greater than fibroblast cells. These results indicate that cyclophosphamide can be a potential anticancer agent.

Mesenchymal Stem Cells (MSCs) are the ideal cell source for transplantation. But different stresses during MSCs in vitro expansion lead to decreased survival rate after transplantation. Therefore, applying practical strategies to enhance their viability in stressful microenvironment is quite necessary. This study aimed to survey effects of HIF-1&alpha-Nrf2-engineered-MSCs secretome on MSCs survival under different stress conditions. Recombinant pcDNA3.1-Nrf2 and pcDNA3.1-HIF-1&alpha were transfected and co-transfected into umbilical cord MSCs (UC-MSCs) using FUGENE HD transfection reagent. After 72 hrs, expression of Nrf2 and HIF-1&alpha were verified by RT-PCR. Different cell groups were exposed to hypoxic, serum deprived and oxidative stress conditions. The HIF-1&alpha-Nrf2-engineered-MSCs secretome was harvested and concentrated. Then, UC-MSCs were cultured in presence of this secretome and their viability was assayed using trypan blue exclusion dye and WST-1 flowing by induction of the same stress conditions. HIF-1&alpha-Nrf2-engineered-MSCs expressed Nrf2 and HIF-1&alpha. HIF-1&alpha-Nrf2-engineered-MSCs indicated a higher survival rate (84.5 ± 5.5%) compared with the control group (55.3 ± 4%). Moreover, the survival rate of UC-MSCs cultured with HIF-1&alpha-Nrf2-engineered-MSCs secretome was 81.6 ± 6% and it was 57.9 ± 4.3% for the control group under the same stress conditions. HIF-1&alpha-Nrf2-engineered-MSCs secretome protects MSCs against oxidative, serum deprived and hypoxic stress conditions.

Background and Recently studies have shown that main compound (Cyclotides) of Viola odorata has an inhibitory effect against proliferation of cancer cells. Also, melatonin reduces size and growth of tumor cells. The main purpose of this study was to compare the inhibitory effect of Viola odorata and melatonin on MDA-MB-468 cells proliferation. In this exprimental study, triple negative human breast cancer cells (MDA-MB-468) were cultured in 96-well plates and incubated with different concentrations of Viola odorata hydroalcoholic extract and melatonin for 24h. Cell viability was measured using MTT assay. In the in vivo study Balb/c mice (6 group, n=6) received subcutaneous injection of 100 µl of cell suspension (4T1 cells) in the left hind flank. The animals were treated with different concentrations of Viola odorata extract (50, 150, 250, and mg/kg) and melatonin (40 mg/kg) for three weeks. Tumor volume changes were measured weekly and weight changes were measured at day 21. The in vitro study showed that different concentrations (30, 40, 60, 80,100,150, and 200 µg/ml) of Viola odorata significantly decreased cell survival in cancer cells (P<0.05). Also, melatonin at concentration of 1mM significantly decreased cell viability (P<0.05). The in vivo study found that melatonin (40 mg/kg) and Viola odorata at 250 mg/ml concentration inhibited increasing of tumor volume after 21 days compared with control group. Treatment with melatonin and Viola odorata extract was found effective in reducing cell survival and tumor volume.

Today induced pluripotent stem cells (iPS) have been recognized as a new and good cell source for cell therapy. In recent years, research in the field of cell therapy and tissue engineering is widely developed. Today there is a potential for the preparation of various types of scaffolds for different cells. In this study, in order to increase surface adhesion properties and cell survival, using of suitable concentration of gelatin modified nanofiber scaffold polylactic acid. Different scaffolds were fabricated by an electrospinning technique and cells morphology and cells viability were evaluated by using a scanning electron microscopy and MTT assay respectively. The results of this study demonstrated that the modified PLA/gelatin scaffold is a more appropriate model for the adhesion, proliferation and cell survival of iPS cells. PLA/gelatin scaffold cause significantly increases in cell attention and cell survival. These findings are an important step in the use of appropriate three dimensional culture in order to treat a variety of diseases.

Breast cancer is one of the leading causes of death in women worldwide. Conventional treatments use cytotoxic drugs which have high numbers of side effects. Currently pharmacologists are searching for novel drugs with fewer side effects and maximum efficiency as breast cancer treatment. The aim of the current study is to clarify the cytotoxicity effect of the recombinant outer membrane inflammatory protein (oipA) of Helicobacter pylori (H. pylori) on a breast cancer cell line.

We purified recombinant H. pylori oipA by Ni–NTA affinity chromatography. Breast cancer cells (4T1) were treated with different concentrations of recombinant oipA for various lengths of time. Cell viability was evaluated by the viability assay (MTT test).

SDS-PAGE analysis showed the expression of an approximately 34000 dalton protein. Statistical analysis showed oipA toxic effects on 4T1 cells at a concentration of 250 µg/ml after 24 h.

These findings suggested that oipA had a direct toxic effect on a breast cancer cell line (4T1) in vitro. The oipA protein might be a new tool for future therapeutic strategies in cancer immunotherapy.

Sulfur mustard (SM) is an alkylating and blistering agent that has readily reacts with a wide range of cellular macromolecules including DNA، RNA and protein. In this study، the effect of SM on cells viability and DNA fragmentation in the human fibroblastic cells was investigated. the HF2FF human skin fibroblast cell line were exposed to various concentrations of SM (180-1000 mM) and then incubated for 24 hours at standard condition. Then، the effect of SM was investigated by measuring percent of viable cells using gentian violet dye assays and DNA fragmentation by agarose gels electrophoresis and diphenylamine reaction. Viability of the cells exposure to 180، 300 and 300 mM SM was 65، 42 and 16%، respectively. DNA fragmentation was increased and represented a smear pattern on agarose gel electrophoresis after exposure to higher concentrations of SM (>180 mM). The effect of SM on viability and DNA damage is dose- dependent. At higher of SM concentrations (>180 µM)، SM alkylates DNA، leading to DNA strand breaks and the nature of the DNA fragments produced suggested that necrotic form of cell death.

Hesperidin is the most common flavonoid in citrus fruit and possesses anticancer proapoptotic and antiproliferative properties for some tumor cells. The present study assayed the effects of hesperidin in the presence and absence of insulin on the survival and apoptosis of Nalm-6 cells، an acute lymphoblastic leukemia cell line that is resistant to chemotherapy. Nalm-6 cells were cultured in RPMI 1640 supplemented with 10% FBS and 1% penicillin and streptomycin. The cytotoxic and apoptotic effects of two concentrations of hesperidin (25 and 50 µM) in the presence or absence of insulin (100 nM) and the cytotoxic and apoptotic effects of wortmannin (10 nM) were assayed using MTT assay and the cell death detection ELISA kit، respectively. Hesperidin decreased the survival rate of Nalm-6 cells in a time dependent manner. After 48 h، cell survival for the 25 and 50 µM concentrations of hesperidin decreased 16. 57± 7. 75% and 29. 33 ± 3. 6%، respectively، in the absence of insulin، and 16. 5 ± 6. 81% and 28. 27 ± 8. 52%، respectively، in the presence of insulin in comparison with the control group (p < 0. 05). Hesperidin also increased apoptotic death in Nalm-6 cells at 25 and 50 µM concentrations 6 and 2. 84 fold in the absence of insulin and 5. 98 and 2. 17 fold in the presence of insulin in comparison to the control group (p < 0. 05). These results indicate the anti-cancer effects of hesperidin by inducing apoptosis and inhibiting cell proliferation in Nalm-6 cells in the presence and absence of insulin. Hesperidin can be suggested as a natural chemo-preventative agent in conjunction with other chemotherapy agents in the treatment of cancer.

Application of herbals subst-ances has been prevalent in the treatment of neoplasm diseases such as cancers. Laven-der has different biological activates and aqueous extracts of the plant has been shown promising future in the treatment of cancer neurodegenerative diseases. The lavender plant and its derivative substances were prepared from the herbarium of Shahid Beheshti University. Fibroblast cells were cultured at RPMI 1640 medium (containing 20% fetal bovine serum and 5% CO2 gas at 37ºC) to achieve appropriate cell numbers. Microscopic studies were performed in the presents of 100 μgml-1 of the extract. MTT assay was applied for cell survival determi-nation in the present of different concentrations of the extract from 0 to 100 μgml-1. Viability test and morphological studies indicated that the population of fibr-oblast cells decreased significantly at the concentrations of 0 to 100 μgml-1 of the lavender extract. Discussion & It could con-clude that application of the aqueous extract of lavender should be carry out under certa-in consideration due to its adverse side effects that might accompany with its eff-ective dosages in cancer therapy. In addi-tion، in vivo studies would be crucial to confirm cytotoxic effects on cancer and normal cells.

Colon cancer is the second lethal cancer after lung cancer. Due to the existence of many side effects and problems in the ways of cancer treatment arising from surgery، chemotherapy or radiotherapy at the present، the need for such ways to be replaced and new drugs is prominent. Usage of herbal essential oils as drug contributes to a major part of traditional and Islamic medicine. This project aims at studying of anticancer activity of Cuminum cyminum essential oils on human colon cancer cell line (SW742). Essential oils are provided by distillation method and their cytotoxicity effects on SW742 cell line were investigated. Human fibroblast cell was used as healthy and control cells. The cells were cultured and different concentrations of each essential oil were induced to cells. After 48 h incubation، cell survival with MTT assay was studied via the statistical methods (software SPSS)، averages and standard deviations were achieved and diagrams were drawn. Findings indicate that essential oil affects on cell survival by two different ways; evaporation part reduces the cell growth enormously of both cancer and fibroblast cells. Soluble part، on the other hand، stimulates cell growth of fibroblast cells، while reduces cell growth of cancer cells. Analytical data provide considerable information about pharmaceutical properties of this plant. Even though، more evaluations are needed to investigate the exact effects of this plant.

Cell-titanium interactions are crucial to the clinical success of bone and dental implants. Mechanical or chemical surface treatment can help the cell attachment to implant surface. The purpose of this study was to investigate the effect of two distinct surface treatment(sandblasted and acid-etched) on fibroblasts cell attachment and viability. In this experimental study the fibroblasts behavior was analyzed on three different titanium surfaces: sand blasted(SB), Acid-Etched (AE)and pure commercial titanium(PCT), in three groups,(N=10). Scanning electron microscopy (SEM) showed distinct microtopographies. Cell morphology and initial attachment were evaluated by SEM. Cell viability was measured by means of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The number of viable cell counts were compared using one way ANOVA Test. SEM observation revealed drastic differences in surface microtopography, with a higher cell density on (SB)than (AE)and(PCT). Cell attachment were much better in the sand-blasted group than others. Cell viability recorded by the sand-blasted group(1.28±0.58) was significantly better than acid-ethed (0.95±0.23) and pure commercial titanium(1.67±0.17).(P < 0.05) Implant surface treatments influence the attachment and viability of fibroblasts(L-929), and sand-blasted treatment seems to be the most favorable surface to compare with acid-etched and and pure commercial titanium surface.