جستجوی مقالات مرتبط با کلیدواژه « ایمونوهیستوشیمی » در نشریات گروه « دامپزشکی »

Ocular squamous cell carcinomas (OSCCs) in cattle has been studied for many years, but no definite etiology has been established. Squamous cell carcinomas (SCCs) may occur in different body parts of cattle. Depending on the location, it can cause an economic loss of varying degrees.

The aim of this study was to investigate the causes of OSCCs in the eye region of cattle.

Sixty tumoral masses taken form 60 cattle with proliferation in the eye region that were collected between the years 2012-2022 were used. These cases were admitted to our department for routine diagnosis. The tissues were diagnosed as OSCC using histopathological methods. The presence of bovine papillomavirus (BPV), one of the causative factors, was investigated using immunohistochemical and polymerase chain reaction (PCR).

Macroscopically masses were nodular or cauliflower-like and fragile and had hemorrhagic surfaces. Considering the keratin pearls, tumoral islands, and squamous differentiation, 20 out of 60 cases were classified as well, 20 as moderately, and 20 as poorly-differentiated OSCCs. 47 of the 60 cases were BPV positive using immunohistochemical methods. However, BPV nucleic acid was detected in only two cases with PCR. Only one of the cases could be sequenced. After phylogenetic analysis, virus strain was identified as BPV-1.

Our results indicated that papillomaviruses can contribute to the development of OSCCs, in both precursor lesions and also advanced stage OSCCs. We found that BPV-1 has a possible causative role; however, more studies are needed to investigate the role of other viral agents and their interaction with secondary factors.

A 4-year-old castrated male Persian cat with three years history of inflammatory and ulcerative lesions in the gingiva and periodontal areas of the right up jaw was referred to a veterinary clinic in Isfahan city of Iran. The clinical signs included gingival ulceration, oral pain, inappetence, ptyalism, halitosis, and chronic loss of weight. The condition was diagnosed clinically as Feline Chronic Gingivostomatitis. Feline Chronic Gingivostomatitis is an immune-mediated inflammatory condition and a painful, debilitating disease of cats. For confirmation of diagnosis, the lesions were sampled for histopathologic examination. Histopathologic examination revealed a chronic inflammatory reaction with infiltration of plasma cells and Lymphocytes predominantly and variable numbers of neutrophils and macrophages. The presence of immune cells in the lesions was followed up by immunohistochemistry for CD3, CD79a, and IgG. The most dominant cells in the lesions were CD79a+ and IgG+ plasma cells. The CD3+ cells incidence concerning the severity of the lesion was high as well.

Using unauthorized tissues in sausage is a common food adulteration in some parts of the world.

This study was designed to compare the accuracy of histochemical and immunohistochemical techniques for the detection of lung tissue in cooked sausage samples.

Samples with different levels of sheep lung tissues (1, 2.5, and 5%) and a control group were prepared and stained histochemically using H&E, Masson trichrome, and Periodic Acid-Schiff (PAS) stainings, and immunohistochemically using two different commercially-available antibodies of TTF1 Pan-cytokeratin.

The highest positive results of lung tissue detection were achieved in sausage samples stained with anti-TTF1 immunohistochemical staining method. Both anti-TTF1 and anti-pan-cytokeratin immunohistochemical techniques detected all contaminated sausage samples treated with 50 g/kg lung tissues. Anti-TTF1 staining method had the highest odds ratio (7.4), followed by anti-pan-cytokeratin method (6.0). Reversely, PAS staining method had the lowest odds ratio (0.21), followed by Masson trichrome method (1.7). Additionally, anti-TTF1 method had the highest (1.8-31.0) confidence intervale (95%), while PAS had the lowest (0.02-2.1). Totally, the odds ratio of lung tissue detected by immunohistochemical methods were higher than those detected by histochemical staining.

This is the first report on the comparison of histochemical and immunohistochemical techniques for lung tissue detection in cooked sausage. Anti-TTF1 immunohistochemical staining proved to be the most useful technique for the detection of unauthorized lung tissue in cooked sausages.

A two-year-old queen was referred to the surgery section of veterinary hospital with lateral recumbency position and the primary diagnosis of dystocia. The queen was highly lethargic and dehydrated, with a pale mucous membrane, a rectal temperature of 37.3 °C, and no vaginal discharge during first inspection. The primary examination revealed a big mass in the abdominal cavity, which was followed by other diagnostic investigations. Due to a huge and encroaching tumor and the patient's owner's refusal to accept any further therapies, the queen was euthanized during an exploratory celiotomy. This report describes a large Hemangiopericytoma (HPCy) in a domestic short-haired queen diagnosed based on physical examination, radiography, ultrasonography, histomorphology, and immunohistochemical reactivity. The results of pathology revealed a whorls around blood vessels and the "fingerprint" pattern of tumor with negative S-100 immunohistochemistry staining.   In dogs, HPCy is a common neoplasm, but it is a seldom finding in cats. There is no previous report of HPCy in the abdominal cavity as observed in the presented case to the authors' knowledge.

This study aimed to investigate the effect of water temperature on the shrimp hepatopancreas of Litopenaeus vannamei by immunohistochemical method. In this research, a total of 50 shrimp with an average length of 23 ± 1 cm and an average weight of 28 ± 0.5 gr were accidentally caught from shrimp farms of the Choebdeh Abadan from Khuzestan province in two different seasons of the year. The shrimp were sampled for the histological and structural study of the hepatopancreas. After killing the shrimp by physical method, the hepatopancreas of each shrimp was transferred to Davidson's solution. The tissue samples were processed by routine histological procedure, using ascending ethanol for dehydration, xylene for clearing, and paraffin impregnation. 4-6 µm sections were taken and stained with hematoxylin-eosin and TUNEL immunohistochemistry technique. Finally, slides were studied by a light microscope equipped with a Dino-lite lens and Dino-capture software. The results indicated that the hepatopancreas structure is a tubular organ that can be affected by water temperature. In the warm season, the diameter of the tubules was smaller than in the cold season (p≤0.05). There was a significant difference in the percentage of apoptosis between the tubule-forming cells in the warm and cold seasons.

Dermatofibroma is a common skin benign cutaneous tumor with many variants based on alterations in the morphology and composition of its diverse elements. The cause of dermatofibroma is unknown, it was his- torically attributed to being a reactive response to some traumatic lesion to the skin which is associated with itching and pain. This tumor usually appears on the limbs, especially the end extremities, in young adults. In the present study, a middle-aged Persian leopard was accidentally killed because of a car incident in the Golestan National Park, Iran. Following necropsy, skin mass on the right toe was observed and after sam- pling was transferred to the laboratory. After routine histopathological and immunohistochemical processes, Dermatofibroma was diagnosed microscopically with non-capsulated, circumscribed, hypocellular dermal nodule in a spiral pattern. According to the results of this study immunohistochemical staining revealed positivity for CD34. There is limited information about the Dermatofibroma in leopards. To our knowledge, this is the first report of Dermatofibroma in the Persian leopard.

Among the birds, chicken was the model used in the majority of studies on germ cells and other birds like quail, turkey and pheasant had some interest but ostrich has been lacking from these researches.

The present study was conducted to confirm the presence of ostrich primordial germ cells (PGCs) in the embryonic blood and to determine the appropriate time for having them in the blood.

Embryos were extracted in embryonic day (E) 6-12, their blood was obtained and the rest of the embryos were processed for histological examinations. Staining by periodic acid-Schiff (PAS) method and immunohistochemistry (IHC) using stage-specific embryonic antigen 1 (SSEA1) and stage-specific embryonic antigen 4 (SSEA4) antibodies (Abs) were used for identification of the PGCs.

While the blood circulating ostrich PGCs were SSEA1-negative and SSEA4-positive, in the presumptive gonads PGCs showed negative immunoreactivity for both Abs. Although the ostrich PGCs were PAS-positive in both blood and presumptive gonad, their PAS-positive contents reduced during development from E 10 to E 12.

E 9, when the hind limb buds appear, is the best time for detecting PGCs in the ostrich embryonic blood.

Diseases affecting the respiratory tract of sheep and goats are one of the most important factors which limit production of these species on a world-wide basis. The main goal of this study was to determine Adenovirus (AdV) antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic goats, using immunohistochemistry (IHC) staining method. For this purpose, the lungs of 402 goats, which were raised in various farms in the Garmsar district and surrounding areas and were brought to the local abattoir for slaughtering between April and September 2016, were examined. Macroscopic pneumonia findings were detected in different lobes particularly in the apical and cardiac lobes of the lungs of 26 goats (%6.46). The rates of mild, moderate and severe consolidations observed in the pneumonic lungs were 59.8%, 26.3% and 11.6%, respectively. Pneumonias were microscopically classified in goats as interstitial pneumonia (n=15) (57.69%), suppurative bronchopneumonia (n=4) (15.38%), bronchointerstitial pneumonia (n=3) (11.53%), and parasitic pneumonia (n=4) (15.38%). A total of 22 pneumonic lungs, excluding parasitic pneumonia, examination with immunohistochemistry (IH) in terms of AdV antigen, were considered. AdV antigen was determined to be (13.63 %) (n=3) by the immunohistochemistry (IHC) method. In conclusion, the presence of viral antigen in lung tissues of goats may indicate that natural pneumonia may be induced by AdV or possibly other species-specific AdVs. Moreover, it is suggested that AdV might have a role in predisposing this species to secondary bacterial infections.

The aim of this study was to find a proper method for improvement of ischemic condition in the rat hind limb and also to observe the efficacy of cell engraftment with alginate/gelatin three-dimensional scaffolds. Eighteen male Wistar rats weighing 200 to 250 g were randomly divided into three groups (n = 6) including a) ischemia group; in which femoral artery was removed after ligation at the distance of 5 mm, b) scaffold group; in which hydrogel scaffold was added to the site of transected femoral artery and c) test group; in which in addition to hydrogel scaffold, mast cells (MCs) were also added (1 × 106 cells). Analysis of capillary density, artery diameter, histomorphometric parameters and immunohistochemistry in transected location were done on day 14 after femoral artery transection. The average number of blood capillary was significantly higher in the test group than other groups. Also, the average number of medium and large blood vessels was significantly higher in the test group compared to ischemia and scaffold groups. Application of MCs through the use of hydrogel scaffolds (alginate/gelatin) can be considered as a new approach in the application of stem cells for therapeutic angiogenesis under ischemic conditions which can improve the angiogenesis process in patients with peripheral artery diseases.

Respiratory tract infections caused by some viruses with cattle origin have been demonstrated in sheep and goats. The main goal of this study was to determine Bovine Herpes virus type 1 BHV1antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic sheep, using immunohistochemistry (IHC) staining method. For this purpose, the lungs of 4079 sheep, which were raised in various farms in the Garmsar district and surrounding areas and were brought to the local abattoir for slaughtering between April and September 2016, were examined. Macroscopic pneumonia findings were detected in different lobes particularly in the apical and cardiac lobes of the lungs of259 sheep (6.35%). The rates of mild, moderate and severe consolidations observed in the pneumonic lungs were 59.8%, 26.3 % and 11.6 %, respectively. Pneumonias were microscopically classified in sheep as interstitial pneumonia (49.8%), suppurative bronchopneumonia (15.7%), bronchointerstitial pneumonia (11.1 %), and parasitic pneumonia (14.3%). A total of 220 pneumonic lungs, excluding parasitic pneumonia, examination with immunohistochemistry (IH) in terms of BHV1 antigen, were considered. BHV1 antigen was determined to be 8.63 % by the immunohistochemistry (IHC) method. In conclusion, the presence of viral antigen in lung tissues of sheep may indicate that natural pneumonia may be induced by BHV1 or possibly other species-specific herpesviruses. Moreover, it is suggested that sheep might have a role in the transmission of this virus to cattle.