جستجوی مقالات مرتبط با کلیدواژه « Zinc oxide nanoparticles » در نشریات گروه « پزشکی »

Microbial infectious diseases are one of the serious health problems that have attracted the attention of the public as a threat to human health all over the world.Zinc oxide nanoparticles due to their various properties such as antibacterial, antifungal, and anticancer are of great interest.

The antimicrobial activityof synthesized un-doped and silver and magnesium-doped zinc oxide nanoparticles with Salvadora persicaextract against different strains of Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa,and Candida albicanswas investigated through two methods of broth diffusion and the minimum microbial inhibitory concentration (MIC).

According to results, MIC of un-doped and silver and magnesium-doped zinc oxide nanoparticles showed 160, 80, 40, and 40 μg/mL on Staphylococcus epidermidis, and 80, 40, 40, and 40 μg/mL on Staphylococcus aureus. These values on Pseudomonas aeruginosawere obtained ˃5120, 640, 320, and 320 μg/mL.

The results of the broth dilution method on Staphylococcus epidermidisand Staphylococcus aureusshowed the presence of silver has a very good inhibition effect on these bacteria. In Pseudomonas aeruginosaand Candida albicansfungus, it has been observed that by increasing the amount of silver and magnesium doping, the inhibitory rate of nanoparticles on Gram-negative bacteria and fungi has increased

Diabetes is a recurrent disease defined as a result of a lack of insulin or resistance to its full capacity. The present study aimed to assess the effect of ginger-zinc oxide nanocomposites on HbA1c and fasting blood sugar (FBS) indexes in diabetic rats.

In this investigation, zinc oxide nanoparticles were synthesized based on the sol-gel method, and ginger extract was obtained using the soxhlet method. Thereafter, the nanocomposite of nanoparticles-ginger extract was synthesized, and the nanoparticles were identified by confirmatory tests. Diabetes was induced in male Wistar rats with a mean weight (250±20 g) by intraperitoneal injection of streptozotocin (60 mg/kg body weight), and the necessary treatments were then given by gavage to the mice for 30 days. At the end of the treatment period, hemoglobin A1c (HbA1c) and fasting blood sugar (FBS) were measured from the collected blood samples.

The results of this research demonstrated that among all the treatments, nanocomposite extract and zinc oxide nanoparticles in low concentration (100+10 mg/kg) have a high ability to reduce blood sugar indicators (HbA1c and FBS). This ability was statistically significant compared to other treated groups (P<0.05).

As evidenced by the results obtained, the nanocomposite of ginger extract + zinc oxide in the concentration (100 +10 mg/kg) was a suitable option for controlling and reducing blood sugar in mice. Furthermore, considering the physiological similarity, the mentioned nanocomposite is recommended as a supplement to lower blood sugar in diabetic people.

 Zinc oxide nanoparticles are one of the nanoscale materials that by inducing the production of reactive oxygen species, DNA damage and apoptosis may have cytotoxic effects on the reproductive system and endanger male fertility. In the present study, we investigated the antioxidant effect of N-acetylcysteine on sperm and biochemical parameters in mice treated with zinc oxide nanoparticles.

24 adult male NMRI mice with average weight (35.45 ± 2.11 g) were randomly divided into four groups (n = 6) of control, zinc oxide nanoparticles (50 mg/kg), N-acetylcysteine (150 mg/kg) and N-acetylcysteine + zinc oxide nanoparticles and treated intraperitoneally for 28 days. Then the mice were anesthetized and their left epididymis was used to evaluate sperm parameters (number, motility and viability). In addition, serum levels of testosterone, LH and FSH, malondialdehyde and total antioxidant capacity were measured. Using SPSS software, data were analyzed by one-way analysis of variance and Tukey's test. Differences among mean values were considered significant at p<0.05.

We observed a significant decrease in the number, percentage of progressive motility and sperm viability, total antioxidant capacity, serum levels of testosterone, LH and FSH in the zinc oxide nanoparticles group compared to those in the control group (p<0.001). While the plasma malondialdehyde level showed a significant increase (p<0.001). Simultaneous treatment with N-acetylcysteine and zinc oxide nanoparticles improved the above parameters significantly. In N-acetylcysteine group, no significant difference was observed in these parameters compared to the control group (p<0.05).

These findings showed that acetylcysteine has a protective role against the destructive effects of zinc oxide nanoparticles on sperm parameters and biochemical factors by reducing oxidative stress, increasing the level of total antioxidant capacity and testosterone levels.

Nowadays, the use of metals such as silver, copper, gold, and aluminum in various forms including nanoparticles as antimicrobial and bactericidal compounds has become popular. The present study aimed to evaluate the effect of zinc nanoparticles on ‌‌‌‌Pseudomonas aeruginosa (ATCC 27853).

In the current experimental study, zinc nanoparticles prepared from Nano Pars Co. ‌‌‌‌Pseudomonas aeruginosa ATCC 27853 was used as the indicator microorganism. The antibacterial activity of nano-particles was compared with benzalkonium using agar diffusion (disk diffusion) and dilution methods. Data were statistically analyzed using SPSS software, version 22 (IBM, Armonk, NY, USA) running one way ANOVA and Tukey test.

The results showed that the addition of zinc nanoparticles to the benzalkonium chloride solvent increased the diameter of the inhibitory zone of Pseudomonas spp. from 9±1 to 19.66 ± 3.05 )P-value =0.001). The MIC and MBC for zinc nanoparticles were 0.15 and 0.24 mg/ml, respectively, while the MIC and MBC for nanoparticles of zinc+benzalconium chloride 10% were 0.03 and 0.04, respectively. This indicates the excellent antibacterial activity of nanoparticles in combination with benzalkonium chloride solvent against Pseudomonas spp.

It seems that the stock of nanoparticles with benzalkonium chloride solvent has bactericidal or growth inhibitory effect against ‌‌‌‌Pseudomonas aeruginosa in different concentrations and has rapid bactericidal effect in concentrations higher than MIC concentration. Zinc oxide nanoparticles can be used to prevent or help treat ‌‌‌‌Pseudomonas aeruginosa infections.

With the increase in the production of nanoparticles, human beings are exposed to its potential dangers. Research has shown the antioxidant effects of zinc oxide at low doses, but long-term exposure to high doses has pro-oxidant effects and influences the function of the male reproductive system, spermatogenesis and fertility. The present study was conducted to investigate the effect of N-acetyl cysteine on testicular tissue changes and spermatogenesis process in male mice treated with zinc oxide nanoparticles.

In this experimental study, 24 adult male mice were assigned to groups of control (1 ml/kg saline), zinc oxide nanoparticles (50 mg/kg), N-acetyl cysteine (150 mg/kg) and N-acetyl cysteine + zinc oxide nanoparticles. In all groups, intraperitoneal injections were performed daily for 28 days. At the end of the treatment, mean weight and volume of testicles, length and diameter of seminiferous tubules, height of germinal epithelium and number of germ cells were evaluated.

In the group of zinc oxide nanoparticles, a significant decrease was observed in volume (38.86±1.48), length (0.89±0.09), diameter (162.69±1.03), and height of germinal epithelium (41.06±1.73) of the seminiferous tubules, number of spermatogonia (4.15±0.11) ×106, spermatocytes (21.45±0.83) ×106, round spermatid (22.31±0.47) ×106, elongated spermatid (21.74±0.76) ×106 and Sertoli cell (3.08±0.10) ×106 and a significant increase was observed in interstitial tissue volume (18.04±1.84) compared to the control group (p<0.001). In the concurrent treatment group, N-acetyl cysteine improved the above parameters compared to the group of zinc oxide nanoparticles (p<0.01). These parameters were not significantly different in the N-acetyl cysteine group compared to the control group.

Based on the results of this study, N-acetyl cysteine was able to prevent the damage caused by zinc oxide nanoparticles on testicular tissue and spermatogenesis.

Ethylbenzene is a volatile organic compound used in many industries, including oil and gas, oil colored and insecticides. Due to the toxic effects of this chemical substance, control and elimination of this vapor is necessary. Photocatalytic degradation is a possible method to remove organic compounds from air. This study was performed to determine the efficiency of photocatalytic removal of ethylbenzene vapor using ZnO nanoparticles immobilized on modified natural zeolite. 

Natural zeolite was first modified with hydrochloric acid and then with diphenyl dichlorosilane. Next, zinc oxide nanoparticles were stabilized on the zeolites. Dynamic air flow and different concentrations of ethyl benzene (25, 50, 100 and 200 ppm) were produced and the removal efficiency of ethylbenzene vapor was investigated using UV/MZe/ZnO. The temperature and relative humidity were set at 25±2°c and 35%. The surface and volume of the pores of the bed were determined by the BET method and surface structure was determined by Scanning Electron Microscope (SEM) and X-Ray Diffraction (XRD).

Evaluations for BET showed the specific surface areas decreased by increasing the amount of ZnO. XRD analysis and SEM images showed that zeolite structure was stabled and nanoparticles was successfully stabilized on Ze. The results showed that the highest removal efficiency (50.8%) by the process of UV/MZe/ZnO at concentration 25 ppm.

The result of this study showed that the Ze/ZnO catalyst may be an applicable and hopeful method to removal of ethylbenzene from air flow under UV irradiation

The presence of drugs in water and wastewater sources poses risks to humans and the environment. These materials are not completely eliminated by conventional water and wastewater treatment methods. This study aimed to investigate the photocatalytic removal of acetaminophen from aqueous solutions by the advanced UV/H2O2/ZnO oxidation method.

In this experimental analytical study, zinc oxide and hydrogen peroxide were used in a 500 ml reactor under UV-C irradiation to remove acetaminophen. The effects of initial parameters of acetaminophen, solution pH, contact time, hydrogen peroxide concentration, and zinc oxide nanoparticle concentration were investigated. Experiments were designed and analyzed using the CCD method by Design Expert 10 software.

Themaximum removal efficiency of acetaminophen was obtained as 94% at pH 7, contact time 8 min, the initial contaminant concentration 62.5 mg/l, catalyst dose 0.0275 g, and H2O2 concentration 2 ml. By changing the time to 1 and 15 minutes, the removal rate decreased by 26 and 76%, respectively, and by increasing the catalyst dose from 0.0275 g to 0.05 g, the removal rate increased by 99%.

In this study, the efficiency decreased with increasing contaminant concentration. The removal efficiency was higher in neutral conditions than in acidic and alkaline conditions. The photocatalytic process (UV/H2O2/ZnO) is highly potent for removing acetaminophen from aqueous solutions.

Biofilms are a community of bacteria on surfaces coated with extracellular polymeric materials, and one strategy to remove biofilms is to use nanoparticles. The aim of this study was to synthesize green zinc oxide (ZnO) nanoparticles and determine its anti-biofilm effects on biofilm gene expression in multidrug-resistant Klebsiella pneumoniae strains.

In this in vitro study, ZnO nanoparticles were synthesized by green method using zinc nitrate solution and Typha latifolia extract. Its physical and chemical properties were confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and FTIR (Fourier-transform infrared spectroscopy) methods. Its antimicrobial and anti-biofilm effects against multidrug-resistant Klebsiella pneumoniae isolates were investigated using minimum inhibitory concentration and plate microtiter methods, respectively. Finally, the effects of ZnO nanoparticles on the expression of wzm biofilm gene were investigated using Real Time PCR.

The synthesized ZnO nanoparticles had a spherical morphology and had a size of less than 100 nm. The results also showed that the synthesized ZnO nanoparticles had significant antimicrobial and anti-biofilm effects against multidrug-resistant K. pneumoniae strains and these nanoparticles significantly reduced the expression of wzm biofilm gene (p<0.05).

The findings of this study showed that the synthesized ZnO nanoparticles have significant antimicrobial and anti-biofilm effects and in the future, with further studies, these nanoparticles can be used as an anti-biofilm candidate.

Resistance of oral pathogenic bacteria to chlorhexidine mouthwash is increasing; therefore, researchers are looking for a replacement of chlorhexidine with suitable herbal mouthwash. This study aimed to investigate the antimicrobial effect of hydroalcoholic extract of Plantago major (a native strain of Khorasan, Iran) with and without zinc oxide nanoparticles on Streptococcus mutans.

In this in vitro study, the samples were tested in two groups of hydroalcoholic extract of Plantago major leaves with and without zinc oxide nanoparticles. To determine the minimum bactericidal concentration and minimal inhibitory concentration, agar diffusion and broth dilution methods were applied, respectively. A concentration of 500 ppm zinc oxide nanoparticles with a 0.4 nm diameter was used continuously in all stages of the experiment. The materials used in this study consisted of a standard strain of Streptococcus mutans (ATCC 25175), chlorhexidine 0.2% as a positive control, and distilled water as a negative control.

The mean diameter of the growth inhibition zone was obtained as 15±1 mm for the hydroalcoholic extract of Plantago major leaves with zinc oxide nanoparticles at a concentration of 1.56 mg/ml. The same extract at the same concentration, however without zinc oxide nanoparticles, showed the mean diameter of growth inhibition zone of 7.67±0.57 mm. The results of the t-test were indicative of a significant difference between the two groups (P-value<0.001).

In this study, the antimicrobial effect of Plantago major leaves extract with or without zinc oxide nanoparticles was well established. This research is peculiar since it revealed the effectiveness of low concentrations of this plant in killing Streptococcus mutans.