جستجوی مقالات مرتبط با کلیدواژه « اینترلوکین-1 بتا » در نشریات گروه « پزشکی »

The prevalence of obesity is one of the most important health issues in the world. The aim of this study was to determine the effect of intermittent resistance training with different intensities on the levels of interleukin-10 (IL-10) and interleukin-1 beta (IL-1β) in obese men.

In this study, 44 obese men (20-30 years old) were assigned into four groups of control, low-intensity intermittent resistance training, moderate-intensity intermittent resistance training, and high-intensity intermittent resistance training. Subjects trained for 12 weeks, 3 sessions per week with three different intensities (80% 1RM with 10 repetitions, 60% 1RM with 13 repetitions and 40% 1RM with 20 repetitions). IL-10 and IL-1β indices were measured 48 hours before the first and 48 hours after the last resistance training session.

A significant difference was reported in IL-10 and IL-1β levels between the intermittent resistance training groups with different intensities. After the training period, IL-1β had a significant decrease in the low (P=0.001), moderate (P=0.01) and high (P=0.001) intensity intermittent resistance training groups. A significant increase in the IL-10 was observed in the resistance training groups with low (P=0.01), moderate (P=0.01) and high (P=0.001) intensity.

According to the present findings, it may recommend intermittent high and moderate intensity resistance training as an effective method for treating obesity and having a favorable effect on its indicators.

Physical inactivity is associated with low-grade systemic inflammation and increased risk of chronic diseases. Beneficial effects of caffeine on adipocytes metabolism and its anti-inflammatory properties have been reported. This study aimed to evaluate the impact of 12 weeks of combined training and caffeine on plasma levels of inflammatory and anti-inflammatory indices in obese men. 

In a quasi-experimental trial, 44 obese men were purposefully and accessibly selected and assigned into four groups each 11 samples: 1) control, 2) combined (resistance-endurance) training, 3) caffeine, and 4) combined training with caffeine. The study subjects received 6 mg of caffeine per kilogram of body weight daily for 12 weeks. The combined training program was performed for 12 weeks, 3 sessions per week. The plasma levels of inflammatory (interleukin[IL]-1β) and anti-inflammatory (IL-10) indices were measured using an ELISA kit. The obtained data were analyzed by dependent t test, 1-way ANOVA, and Tukey post hoc test at the significant level of P<0.05.

The results showed that 12 weeks of combined training, caffeine, and combined training-caffeine significantly reduced IL-1β levels and increased IL-10 in obese men at posttest compared to the pretest (P=0.001). Also, changes in IL-1β and IL-10 levels were greater in the combined training-caffeine group (P=0.001). 

 Combined training with caffeine positively affects body composition and improves inflammatory and anti-inflammatory indices in obese people.

One consequence of diabetes mellitus is increased inflammation, which is exacerbated by training. The objective of this study was to evaluate the effect of eight weeks of aerobic moderate-intensity continuous training on Interleukin-1 beta, Interleukin-13 responses in male Wistar diabetic rats.

36 diabetic rats were randomly assigned into 3 equal groups (control (C), diabetic (D), and diabetes + training (D + T)), and subjected to eight weeks of aerobic moderate- intensity continuous training intensity at the first: the10 min on 10m/min and at the final: 55min on 26m/min speed. Interleukin-1 and interleukin-13 were measured in soleus muscle tissue to examine the pro-inflammatory and anti-inflammatory indicators. ANOVA and Scheffe post hoc tests were used for data analysis.

Induction of diabetes caused a significant increase in IL-1β and IL-13 (P<0.001) and continuous exercise intervention also significantly reduced IL-1β levels in diabetic rats (P = 0.02). However, there was no significant change in IL-13 in D + T group compared to D group (P = 0.985).

According to the results of this study, the effect of aerobic exercise is probably a positive effect on reducing the pro-inflammatory index of IL-1β and no effect on the anti-inflammatory index of IL-13 in diabetic rats. However, definitive confirmation of these results requires further research in this area.

Many investigations revealed that the inflammatory process induced by cerebral ischemia/reperfusion causes brain damages and cognitive impairments. On the other hand, Menaquinone-4 (MK-4) is one of the important vitamin K2 types that has anti-inflammatory effects. Therefore, in this study, we investigated the effect of administration of MK-4 on the level of gene expression of proinflammatory cytokines following global ischemia/reperfusion in the hippocampus of male Wistar rats.

In this research, 20 adult male Wistar rats (250-300 g) were randomly selected in 5 experimental groups and studied: control (intact), sham (surgery without carotid artery occlusion), ischemia/reperfusion, ischemia/reperfusion + intraperitoneal (i.p.) injection of DMSO as MK-4 solvent, treatment (ischemia/reperfusion + i.p. injection of MK-4). For induction ischemic model, common carotid occlusion was performed for 20 minutes. In the treatment group i.p. injection of 200 mg/kg MK-4 was done 20 minutes after obstruction (immediately and 2 hours after reperfusion). 24 hours after reperfusion, mRNA expression level of TNF-α, IL-1β and IL-6 were assessed.

I.p. administration of MK-4 could significantly decrease mRNA expression level of TNF-α (p < 1.15), IL-1β and IL-6 (p < 0.001) induced by ischemia/reperfusion.

The findings of this study show that MK-4 administration following cerebral ischemia/reperfusion could diminish the expression of the pro-inflammatory factors in the hippocampus and maybe cause neuroprotective effects. Received.

Increased inflammation is a consequence of diabetes. Accordingly, the use of herbal medicine as a solution has been suggested. The objective of this study was to evaluate the effect of two months of chia supplementation in conjunction with inflammatory and anti-inflammatory responses in male Wistar diabetic rats.

36 diabetic rats were randomly assigned to 3 equal groups (control group, diabetes group, diabetes + chia group) and were subjected to 8 weeks of supplementation. Interleukin-1 and interleukin-13 were measured in soleus muscle tissue to examine the inflammatory and anti-inflammatory indicators. ANOVA and Tukeychr('39')s post hoc test were used for data analysis.

Although chia supplementation had aggravating effects on IL-1ß compared with the diabetic group, this effect was not statistically significant (p = 0.99). Also, IL-13 protein expression increased significantly in the diabetic control group by about 51% (p = 0.001), and in the diabetic group with chia supplementation by about 50% (p= 0.001) compared to the healthy control group. There was no significant difference between the two groups.

It seems that two months of chia supplementation with this dose and period of consumption could not have a positive effect on anti-inflammatory factors in male diabetic rats. Confirmation of this finding requires further research in this area.

Obesity is associated with chronic inflammation in obese subjects, which leads to an increase of inflammatory cytokines. This study aimed to evaluate the effect of different intensity circuit resistance training on levels of interleukin 1 beta and interleukin-10 in obese men.

In a semi-experimental trial during May to August 2020, 44 obese men from Tehran city were selected and randomly divided into 4 groups including 1) control (n=11), 2) low-intensity circuit resistance training (n=11), 3) Moderate intensity circuit resistance training (n=11) and 4) High-intensity circuit resistance training (n=11). Resistance training was performed in different intensities including1) High-intensity circuit resistance training: three sets of 10 repetitions with 80% 1RM 2) Moderate intensity circuit resistance: three sets of 13 repetitions with 60% 1RM and 3) low-intensity circuit resistance training: three sets of 20 repetitions with 40% 1RM, three sessions per week for 12 weeks. Also, the control group had their daily life during the 12-week research period and were prohibited from participating in regular exercise. Serum interleukin-1 beta (IL-1β) and interleukin-10 (IL-10) levels were measured using an ELISA kit. Data were analyzed with covariance analysis at P<0.05.

Twelve weeks of low, Moderate and high intensity circuit resistance training significantly decreased IL-1β levels (P=0.001) and increased IL-10 levels in obese men (P=0.001). The decrease of IL-1β was significant in the high-intensity training group compared to low intensity (P=0.009) and moderate-intensity training groups (P=0.046). Also, the increase in IL-10 levels was significant in the high-intensity training group compared to the low-intensity (P=0.002) and the moderate intensity training group compared to the low intensity training group (P=0.004).

According to our findings, resistance training has a positive effect on inflammatory and anti-inflammatory factors in obese men, and high-intensity circular resistance training had more benefits.

The purpose of this study was to investigate the effect of concurrent training on pre-inflammatory (IL-1β, TNF-α) and anti-inflammatory (IL-10) cytokines gene expression in women with type2 diabetes.

18-patients (age30-38, >130 glycemic index) were selected, randomly, and divided into control (n=6) the concurrent training(diabetes, n=6) and the concurrent training (healthy, n=6)  groups. The concurrent training protocol consisted of 3 sessions resistance training per week, 8sets with 80% one maximum repetition( and the endurance training preformed with 30- minutes running (3sets×10 minutes) on a treadmill with 70-80 maximum heart rate(70-80 MHR), immediately. The leukocyte’s IL-1β, TNF-α and IL-10 genes expression determined by the Real time-PCR technique. The quantitative expression of the cytokines gene was calculated using the 2-ΔΔ CT method. The between- groups differences in variables were determined by independent t-test (permutation test) through REST software and independent one-way ANOVA.

The results showed that IL-1β, TNF-α mRNA genes expression reduced significantly after the concurrent training in both the training groups in comparison to the control group (P<0.05). In addition, the results also showed that IL-10 mRNA gene expression was not expressed in leukocytes after the concurrent training in both training groups in comparison to the control group (P>0.05).

In conclusion, the results suggest that the concurrent training modulate IL-1β and TNF-α mRNA genes expression significantly in diabetic women but could not change IL-10 genes expression. This type of exercise training seems to be more effective in reducing the expression of pro-inflammatory cytokines than in enhancing anti-inflammatory cytokines.

Type 2 diabetes mellitus (T2DM) is an inflammatory disease with alterations in immune system. Remarkably, cytokine imbalance plays an important role in pathogenesis of T2DM. The purpose of this study was to determine the serum levels of TNF-α, IL-1β and IL-10 and the effects of sitagliptin on the level of these cytokines in patients with T2DM.

Blood samples were collected from 60 T2DM patients and 30 healthy control subjects (HCs). T2DM patients were divided into two subgroups based on their treatment; with sitagliptin treatment (100 mg/day, n=30) for 8 months and without sitagliptin treatment (n=30). The serum levels of TNF-α, IL-1β and IL-10 cytokines were assessed using ELISA method. ANOVA with posthoc Bonferroni were applied to statistical analysis.

The serum level of TNF-α was significantly higher in T2DM patients without sitagliptin compared to HCs (p=0.002). Whereas, the serum level of TNF-α was significantly lower in patients with sitagliptin compared to patients without sitagliptin (p=0.01). The serum level of IL-10 showed a significant reduction in patients without sitagliptin compared to HCs (p=0.003). On the other hand, a significant elevation was observed in serum level of IL-10 between T2DM patients with and without sitagliptin (p=0.002). Considerably, no significant difference was observed in serum level of IL-1β between T2DM patients with and without sitagliptin and also in comparison to HCs.

Sitagliptin treatment significantly decreased the level of pro-inflammatory TNF-α and increased anti-inflammatory IL-10 in T2DM patients. It seems that sitagliptin had an anti-inflammatory effect on immune system of the patients.

Exercise-related stress changes the amount of cytokines produced. Dietary supplements can accelerate the muscle recovery process. Q10 is one of the most effective and nutritional supplements that is prescribed to reduce the inflammatory-oxidative damage. Therefore, this study aimed to investigate the effect of Q10 supplementation and recovery in water on serum levels of IL-6 and IL-1Beta in active girls following eccentric exercises.

In this quasi-experimental study, 32 active female students aged 19-21 years old were randomly assigned to four groups of 8 people: placebo, exercise + recovery, exercise + Q10 and exercise + recovery + Q10. The protocol was 60-minutes sessions of eccentric exercises three days a week for 3 weeks. Blood samples were taken at three points during the study: before the intervention, after eccentric exercises and after recovery.

The use of Q10 supplementation and recovery in water following eccentric exercises caused a significant difference in serum levels of IL-6 in the exercise + recovery, exercise + Q10 and exercise + recovery + Q10 groups compared to the placebo group (P=0.035). However, no significant change was observed in the levels of interleukin-1 beta in the exercise + recovery, exercise + Q10 and exercise + recovery + Q10 compared to the placebo group (P= 0.349).

Q10 supplementation along with a vigorous eccentric exercise program had beneficial effects on interleukin-6 inflammatory factor. Therefore, due to its antioxidant properties, Q10 supplementation is recommended as a non-pharmacological approach to helping muscle soreness in active individuals. However, this supplement does not result in reduction of some inflammatory factors, including interleukin-1 beta.

Air pollution PM exposure associated with increased inflammation and decreased cellular immune function. The innate immune system is one of the first lines of defense against inhaled air pollution and is characterized by activation of lung key signaling pathways. One pathway is initiated by the activation of toll-like receptors (TLRs) that associated with airway cells culminates in the translocation of nuclear factor-kappa B and other transcription factors to the nucleus, and therefore initiation of altered signaling of pro-inflammatory mediators such as IL-1β and TNF-α. The purpose of this study was to investigate the effect of 4 weeks of aerobic training after long-term inhalation of black carbon black particulate matter on IL-β lung tissue in male rats.
The present study was conducted experimentally in October and November 2015 at the Sport Physiology Laboratory of Tarbiat Modares University of Tehran. 12 adult male Wistar rats (279.29±26.97) were divided to two groups: A: Daily, two hours inhalation of carbon black PM10, n=6), B: 60 minutes aerobic exercise activity, 50 percent average peak velocity, 15 minutes after inhalation of carbon black PM10. The gene expression of IL-1β was analyzed in lung tissue by real-time-PCR and Pfaffl formula. In order to determine the significant differences between groups independent t-test were used.
Although, there were no significant differences among the A and B groups, however, the mean of relative gene expression of IL-1β in group B was slightly lower than group A.
Four weeks regular aerobic exercise activity did not worsen lung tissue inflammation after long-term inhalation of carbon black PM10. It seems regular exercise training mitigate pulmonary inflammatory responses and in part, decrease the risk of pulmonary diseases.

Periodontal diseases are multifactorial conditions that are induced in the presence of bacteria and under the influence of environmental and genetic factors. Management of environmental factors might help prevent such diseases. The aim of this study was to investigate the relationship between interleukin-1α، β gene polymorphism and chronic periodontitis (CP). This case-control study included 36 subjects with chronic periodontitis referred to Periodontology Department of Khorasgan Dental School and 36 periodontally healthy controls، with an ethnic origin of Isfahani Iranian race. Extracted DNA from peripheral blood was used to evaluate interleukin-1α، β gene polymorphism. Data were analyzed using chi-squared and Fisher’s exact tests (α=0. 05). There was a significant association between IL-1α -889، 2/2 genotype and CP and between IL-1α -889 allele and periodontal health (p value = 0. 03). There was no significant association between IL-1β +3954 genotype and CP (p value = 0. 14). There was no significant association between IL-1β +3954 alleles and CP (p value = 0. 06). Based on the results of the present study، association between IL-1α -889 polymorphism in the population presented here confirms this gene as a risk factor for CP. However، the lack of any association between IL-1β +3954 polymorphism and CP makes it dubious to use this gene as a marker of susceptibility to CP.

Statement of Problem: Periodontitis is a chronic multi-factorial infectious disease,characterized by irreversible destruction of collagen fibers and other matrix constituents of the gingival tissues, periodontal ligament and resorption of the alveolar bone around the teeth with formation of periodontal pocket. Cytokines such as IL-1β are one of the components of host’s immune system and seem to play an important role in periodontitis The aim of this study was to determine the concentration of IL-1β as a per-inflammatory cytokine in the saliva of periodontally involved patients (generalized aggressive periodontitis and mild to moderate periodontitis) and subjects with normal periodontium. In this experimental study, unstimulated saliva of 24 patients with mild to moderate chronic periodontitis, 15 patients with aggressive periodontitis, and 23 subjects with healthy periodontium was collected. The concentration of IL-1β was measured in the saliva samples by ELISA. Mann-Whitney test was used for analysis of data. The results of this study showed that there was a significant difference between mean level of IL-1ß in generalized aggressive periodontitis vs. control groups and chronic mild to moderate periodontitis vs. control groups (p <0.05). The findings of the present study showed that the mean concentration of IL-1ß in the saliva of periodontally involved patients was greater than that of healthy subjects and this cytokine can be agood marker for determining the status of periodontal tissues.