جستجوی مقالات مرتبط با کلیدواژه « پربیوتیک » در نشریات گروه « شیلات »

Contaminants in aquatic ecosystems cause tissue damage in fish. In some cases, the effects of pesticide degradation on non-target organisms (aquatic) are greater than on target organisms (pests), which in turn causes faster and more aquatic deaths. The use of safety stimuli seems to be essential. The aim of this study was to investigate the effect of different prebiotic levels of Pleurotus ostreatus on histological characteristics of tilapia (Oreochromis niloticus) exposed to chlorpyrifos. This research was conducted in the fall of 2019. For this purpose, 120 tilapia fry were divided into 4 treatments for 42 days. Then, 0.5 ppm concentration of chlorpyrifos was added to each group for 16 days. At the end of the period, the fish were anesthetized with clove anesthetic solution (220 mg / l) and their liver tissue and gills were isolated for histological studies.  The results showed that the treatments exposed to 0.5 ppm chlorpyrifos (poisoned control without prebiotics) had the most destructive effect on liver and gill tissue. However, combined prebiotic treatments of Pleurotus ostreatus and chlorpyrifos improved tissue damage, indicating detoxification, and a concentration of 0.2 ppt Pleurotus ostreatus had the best performance against tissue damage to the liver and gills of tilapia, indicating improved resistance.

Increasing the growth and immune system of beluga sturgeon (Huso huso) which is a valuable species is an important aim for aquaculture so The purpose of this study was to investigate the effect of pectin and L. plantarum on growth performance and growth genes of juvenile beluga sturgeon (Huso huso). Triplicate groups of beluga juveniles each (15 ± 3 g) were placed in 9 oval tanks (400) and fed with the 107 cfu g-1, 108 cfu g-1, pectin (1%), pectin (2%), 107 cfu g-1 and 1% pectin, 107 cfu g-1 and 2% pectin, 108 cfu g-1 and 1% pectin, 108 cfu g-1 and 2% pectin and control group. The trial lasted 8 weeks. At the end of experiment biometery was done and brain and liver tissue were separataed. Results showed that although pectin and L. plantarum could enhance growth factors separately,the highest FW, GW and SGR was observed in fish fed 108 cfu g-1 and 2% pectin which had significant differences with others treatments and control (P<0.05). Also the highest genes expression was in this treatment and the lowest in control. In general, pectin and L. plantarum could significantly affect growth performance in juvenile blugae

The increase of heavy metals disturbs the aquatic ecosystems by affecting the physiological, biochemical parameters and cells of the aquatic organism and causes a decrease in the immune function of fishes. The aim of this study was to determine toxic effects of silver sulfate toxicity on some blood indices of Nile tilapia fish (Oreochromis niloticus) fed with different levels of oyster mushroom (Pleurotus ostreatus) prebiotic. 120 Nile tilapia fry were fed for 6 weeks in 4 treatments with 0, 0.05, 0.1 and 0.2% of prebiotics Oyster mushrooms. At the end of feeding period, the experimental samples were exposed to silver sulfate for 14 days, and sampling was done at two times (at the end of the feeding period, at the end of the toxicity exposure) and hematological indices were measured. The results showed prebiotic alone had no significant effect on RBC, MCV, hematocrit and hemoglobin (P<0.05). However, the amount of white blood cells, MCH and MCHC significantly increased in the treatments fed with prebiotic 0.2 and exposure. In general, Oyster mushroom powder stimulated the non-specific immunity of tilapia and also reduced the destructive effects caused by silver sulfate on blood indices. In general, the amount of 0.1 and 0.2% oyster mushroom in the exposed treatments was able to improve the normal blood indices of Nile tilapia.

Intensive aquaculture and commercialization production are believed to be needed for improving fish health status and probiotics and prebiotics are candidates to achieve this goal. The aim of this study was to evaluate the single and combined effects of dietary probiotic with prebiotic on intestine histology and blood biochemical parameters of Sobaity fingerling (Sparidentex hasta). For this purpose, a total of 180 Sobaity fingerlings were prepared with an average weight of 35.14±0.8g from the Mariculture Research Station of South Iranian Aquaculture Research Center. This study was carried out in a completely randomized design with four treatments and three replications (15 fish per each replication) in fiberglass tanks with 300 liters volume. Fish were fed with feed containing without supplementation (control group), probiotic 106 CFU/g feed (treatment 1), 0.5 and 1 percent prebiotic 106 CFU/g feed (treatment 2 and 3) at satiation for a period of 8 weeks. At the end of the experiment, the intestine and blood samples were collected. The results showed that combination of dietary probiotic with prebiotic did not change intestine histology of Sobaity fish (P>0.05). At the end of the experiment, the dietary prebiotic and probiotic also did not change blood biochemical parameters (glucose, triglycerides, cholesterol, HDL, LDL and VLDL) (P>0.05). This study showed that combination of probiotic with prebiotic at the used amounts had no significant effects on intestine histology and blood biochemical parameters of Sobaity fish. However, single supplementation of probiotic causes the elevation of villi length.

The aim of this study was to investigate the effects of apple pomace (Malus pumila) derived pectin (APDP) on rainbow trout (Oncorhynchus mykiss). Therefore, 300 rainbow trout with an average weight of 3.56 ± 0.007 were fed diets containing 0.5, 1 and 2% pectin per kg of diet and the control group was fed a diet without pectin for 6 weeks. At the end of the experiment, hematological and biochemical indices were evaluated based on standard formulas and means were analyzed using Tukey test. Based on the results obtained from hematological indices, the treatment fed with 0.5 and 1% of pectin showed the highest levels of hematocrit, hemoglobin and numbers of white blood cells significantly different from those of control treatment (p < 0.05). In terms of other blood indices (erythrocytes, MCV and MCH), no significant difference was found between the experimental treatments and control group (P>0.05). Feeding fish with pectin-containing diets significantly increased albumin index and decreased cholesterol, glucose and cortisol indices (p < 0.05). In case of total protein, globulin and triglyceride, no significant difference was observed between different levels of pectin and control group (P>0.05). Therefore, it can be concluded that the use of pectin in the diet of rainbow trout leads to the improvement of some hematological and biochemical indices

This study aimed to investigate the effect of different levels of prebiotic Pleurotus ostreatus on hematological indices of Nile tilapia, Oreochromis niloticus exposed to chlorpyrifos toxin.A total of120 fish were distributed for 42 days in 4 treatments (each with 3 replicates), including control (1), no prebiotic fungi, (2) food containing 0.05, (3) food containing 0.1 and (4) food contained 0.2% prebiotic. Then, 0.5 ppm of Chlorpyrifos was added to each group for 16 days and their blood parameters were examined. Based on the results, prebiotic alone had no significant effect on erythrocyte count, mean cell volume (MCV), hematocrit, hemoglobin, white blood cell (p < /em><0.05) but exposure to Chlorpyrifos and prebiotic in combination increased the mentioned factors compared to the control and prebiotic treatments alone. However, the levels of white blood cells and erythrocytes, hemoglobin, hematocrit, and MCHC were higher than the other treatments in the combination of 0.2 and 0.1% prebiotic and Chlorpyrifos. It is concluded that the 0.1 and 0.2% prebiotic level could have the best effect on the blood indices of Nile tilapia.

The objective of this research was to investigate the effects of three commercial prebiotics A-Max, Celmanax and Ultra (each of 0.5, 1 and 1.5 g/kg diets) on growth, feed performance and blood biochemical compounds of beluga juvenile.

900 fish with average weight of 21.5± 0.89 gr feeding was performed in 9 treatments (which on with three replicate) for 60 days. Control treatment was without supplementation. Fish were fed to satiation triple daily of 5% body weight. At the end of this period, the feed and growth performance were measured.

There was a significantly higher final body weight in ultra 0.5 (90.00± 21.78 g) compared with control (37.50± 9.60 g). The highest and lowest of food conversion were in treatments control and ultra (0.5), respectively. The results showed that the highest Fat and protein efficiency ratio was obtained in Ultra 0.5 (2.19± 0.77 and 7.37± 2.60) and lowest in control. Generally, the best treatments were in groups: Celmanax (dose of 0.5), A-Max (dose of 1.5) and Ultra (0.5). In addition, after determining the proper dose, blood samples were collected fish in the above-mentioned treatments. Based on the results of blood biochemistry parameters, there were no significant difference AST, ALP and ALT activity, so that the highest amount was obtained in the control. There were no significant differences in protein and cortisol experiment groups.

The results showed that the use of prebiotics in juvenile sturgeon diet has positive effects on feed performance and blood biochemical parameters.

Aim of this study was to investigate the protective effect of pre-treatment of bacteria pediococcus acidilactici and oligosaccharide raffinose on carcass composition of Goldfish (carassius auratus) in exposure to nano silver. Accordingly, 250 fishe with a average weighing of 26.3±0.18 fed for 6 weeks in four treatments and each treatment with three replications including diet without food supplement (control 1), probiotic diet of bacteria with a concentration of 107 colonies per gram (treatment 2), food containing probiotic raffinose (1 g/kg) and synbiotic (treatment 4). After the end of the feeding period, 50% of the nano-silver concentration (0.5 mg / L) was added to the treatments for 14 days. At the end of the 14-day exposure, blood and carcass sampling was performed. Analysis of the data showed that there was no significant difference between moisture content, ash, protein and carcass fat (P <0.05). According to the results obtained from the tested indices, Rafinose supplementation had the greatest effect on carcass fat. Among the supplements, the synbiotic food with effect on carcass protein, carcass ash and carcass moisture, among food supplements has more effect.

Prebiotics are non-digestive food ingredients which beneficially affect the host by selectively stimulating the growth and activity of health-promoting bacteria in the intestinal tract. This experiment was conducted to evaluate the effect of dietary supplementation of galactooilgosaccharide on the growth performances, survival and several of innate immunity parameters of rainbow trout (Oncorhynchus mykiss) larvae for 60 days. In this experiment, 360 of larvae (with average weight of 0.13±0.01g) in 4 treatments and 3 replicates (n=30 in each replicate) in a completely randomized design was divided and fed with diets containing 0, 1, 2 and 3 percent galactooligosaccharide respectively. The present results showed that highest WG, SGR and PER were observed in the diet containing 2 and 3 percent of dietary galactoologosaccharide which had a significant difference compared with other treatments (p

Improved growth, nutritional efficiency and resistance aganist diseases are among the most important goals of the aquaculture industry. Sustainable aquaculture development requires the use of novel techniques mainly including the use of probiotics to optimize the physiochemical factrors of rearing environment and improving the animal performance. In this regard, the isolation of biologically active bacteria from the intestines of farmed fish and the use of commercial products of such beneficial bacteria as an important strategy for biocontrol and bio-enhancement of aquatic animal performance would play a very important role in achieving aforementioned objectives of the industry. However, using ingredients which are not of nutritional importance to aquatic animal per se (prebiotics), while are beneficial to probiotic bacteria (synbiotics) can be effective in improving immune system of aquatics.

This experiment was conducted to evaluate the effect of A-Max Ultra prebiotic at five concentrations of 0 (control), 0.3 (T1), 0.5 (T2), 0.7(T3) and 1(T4) g.kg-1 on growth parameters, feed utilization, survival rate and resistance against the challenge tests of common carp larvae were compared for 60 days. For this purpose 600 Common carp F1 larvae with average mean weight of 1.3±0.273g and (±SD) were obtained and transferred to the laboratory. After 14 days adaptation to the laboratory conditions the fish were randomly divided into five treatments with three replicates (four experimental treatment and the control). The results revealed that, the highest of Growth parameters were observed in the diet containing 0.5 and 0.7 g.kg-1 A-Max Ultra prebiotic supplement and the highest of feed indices were observed in the diet containing 0.3 g.kg-1 prebiotic supplement that a significant difference was observed between treatments and the control treatment (P

The aim of this study was to evaluate the effect of mannan oligosaccharide (MOS; activeMOS®) on haematological and biochemical parameters of Rainbow trout (Oncorhynchus mykiss) juveniles. Fish fed during 60 days with a basal diet supplemented with 1، 2. 5 and 4 g/kg g kg-1 MOS. Blood samples were collected from caudal vein of 36 apparently healthy fish at the end of trial and Data was analysed by regression analysis and pearson correlation test. There were no significant differences in serum enzymes activity between treatments (P>0. 05). There were no differences in the glucose، cholesterol، triglyceride، uric acid، total protein and bilirobine between treatments (P>0. 05). but the level of WBC significantly higher in the group fed 1g/kg g kg-1 MOS than other groups (P<0. 05). A non significant elevation of haematocrit، haemoglobin level and lymphocyte was found in the fish fed diet 1 g/kg g kg-1mannan oligosaccharide (P>0. 05). The results indicate that dietary administration of mannan oligosaccharide at the level of 1 g/kg g kg-1 can positively influence on some blood parameters of juvenile rainbow trout.

Effect of dietary prebiotic mannan oligosaccharide and β-1،3 glucan on growth performance، body composition and immunity response of rainbow trout (Oncorhynchus mykiss) were investigated for a six week culture period. Four feeding strategies were set، including feeding prebiotic-free diet continuously (control)، feeding dietary prebiotic continuously (T1)، feeding dietary alternately (one week prebiotic+one week control diet) (T2) and 2 Day prebiotic+5 Day control diet (3). The experiment carried out in 500 liters Plastic tanks. 32 juveniles rainbow trout with initially average weight 19. 6 ± 0. 06 were stocked in tanks and fed up a day. The results showed no significance difference was observed on growth performance and feeding indexs (P>0. 05). There was significant differences in composition carcass (P<0. 05). The highest protein content was observed in the control treatment and maximum fat was observed in treatment 2. There were significant differences in the serum lysozyme activity (P<0. 05). Continuous administration of prebiotic mannan oligosaccharide and β-1،3-glucan showed significantly higher serum lysozyme activity (53. 89) than those in the control group (16. 88). The result indicated that continuously applying mannan oligosaccharide and β-1،3- glucan into the diet compare to discontinuous administration caused improved growth performance and immunity response for rainbow trout.

Current use of prebiotic as indigestible feed additive improves health statues of host، instead of drug and chemical material in aquaculture. The aim of this experiment was to investigate the effect of 2g. kg-1 prebiotic immunogene on hematological، biochemical and some serum enzymes parameter of rainbow trout after 7 weeks feeding. Hundred and twenty rainbow trout (81. 65 ± 1. 49 g) After checking the health status and acclimatized to laboratory condition for 10 days، they were equally stocked in six fiberglass tanks (1000 L) assigned to two levels (0 and 2 g kg-1) of Immunogen (commercial prebiotic، ICC Co; USA) with three replicates. During the experimental trial (7 weeks) fish were hand-fed (2% of BW) twice daily (09:00 and 17:00). At the end of trial the blood was collected using a hypodermic syringe from the caudal blood vessels and was used to evaluate the hematological and biochemical parameter. The results of this experiment indicated that some hematological parameter such as RBC، Hb، MCH، MCV and MCHC were not affected by immunogen (p>0. 05)، but the Hct and WBC were increased in rainbow trout immunogen (P 0. 05). Considering serum biochemical parameter it was observed that in the group fed with prebiotic immunogen increased the level of glucose (P<0. 05)، Although the level of triglyceride and serum total protein were not affected، but the level of serum cholesterol was increased in group fed with prebiotic immunogen (P>0. 05). The results indicated that administration of immunogen no effect on ALT، AST، LDH and ALP levels. These results indicate that fish blood parameters and serum biochemical profiles could be affected by immunogen، which should be taken into account in future studies.

The aim of this study was to evaluate the effect of mannan oligosaccharide and β-1,3 glucan (TechnoMos®) on hematological and biochemical parameters of rainbow trout (Oncorhynchus mykiss) juveniles.The fish fed during 55 days with a basal diet supplemented with 1.5, 3 and 4.5 g/kg TechnoMos®. Blood samples were collected from caudal vein of 84 apparently healthy fish (average weight 40.09±1.32 g) at the end of trial. There were no significant differences in serum enzymes activity like ALT, AST and LDH levels among treatments (P>0.05). While ALP was decreased significantly at 4.5 g/kg TechnoMos® supplementation (P<0.05). The result showed that haematocrit and haemaglobin levels were elevated significantly in 3 g/kg TechnoMos® (P<0.05). The levels of albumin, total protein and cholesterol were significantly higher in the group fed 1.5 g/kg TechnoMos® than other groups (P<0.05). The results indicated that the prebiotic mannan oligosaccharide and β-1, 3 glucan could influence some blood parameters in rainbow trout juveniles

The aim of the present study was to investigate the effects of dietary oligofructose (1, 2 and 3%) on blood profiles of beluga Huso huso juveniles (18.77±0.76g). After 7 weeks feeding on experimental diets hematological parameters, metabolic products (cholesterol, glucose and total protein) and serum enzymes (lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase) were measured. Compared to the control group (0% oligfructose), oligofructose had no effects on red blood cell counts (RBC), mean corposcular volume (MCV), mean cellular hemoglobin concentration (MCH) or mean cell hemoglobin concentration (MCHC) (P>0.05). However, hematocrit (Hct), hemoglobin concentration (Hb) and leucocyte counts (WBC) were significantly higher in fish fed 2% oligofructose (P<0.05). Although serum glucose and total protein remained unaffected, cholesterol was significantly lower in the 2% oligofructose group (P<0.05). Our results showed oligofructose had no significant effects on serum lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase (P>0.05). These results indicate an immunomodulatory effect and the potential to reduce serum cholesterol levels in beluga sturgeon.